LABORATORY EXERCISES FOR MALE REPRODUCTIVE SYSTEM

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Wilfrid Anderson
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1 LABORATORY EXERCISES FOR MALE REPRODUCTIVE SYSTEM

these cells are visible adjacent to the limiting membrane of the seminiferous tubules THE SERTOLI CELLS (SUSTENTACULAR CELLS) EXTEND

2 Slide #101 (1096). Testis, rat. sustentacular ( Sertoli ) cells Nuclei of Sustentacular cells Leydig cells Spermatogonia Spermatocytes Spermatids pale nuclei of these cells are visible adjacent to the limiting membrane of the seminiferous tubules THE SERTOLI CELLS (SUSTENTACULAR CELLS) EXTEND THE FULL THICKNESS OF THE WALL OF THE SEMINIFEROUS TUBULE. IN OTHER WORDS, THE SERTOLI CELLS EXTEND FROM THE BASEMENT MEMBRANE TO THE LUMEN OF THE SEMINIFEROUS TUBULE. THE FUNCTION OF THE SERTOLI CELL IS SUPPORT FOR THE DEVELOPING GERM CELLS.

3 Horse Sperm This EM at

4 Slide #101 (1096). Testis, rat. Nuclei of sustentacular or Sertoli cells Myoid cell nucleus Spermatogonia Spermatocytes Spermatids Spermatocyte seminiferous tubule lumen Leydig cells

WHEN A SPERMATOGOIUM CELL IS SIGNALED TO UNDERGO MEIOSIS INSTEAD OF MITOSIS, THE CELL HAS REPLICATED ITS DNA TO BECOME 4N AND HAS

5 Slide #101 (1096). Testis, rat. seminiferous tubule spermatids Meiotic figures (metaphase plates) Primary S. Secondary S. spermatid Myoid cell Spermatocytes Sustentacular cell nuclei or Sertoli cells Leydig cells Spermatogonia Secondary spermatocytes WHEN A SPERMATOGOIUM CELL IS SIGNALED TO UNDERGO MEIOSIS INSTEAD OF MITOSIS, THE CELL HAS REPLICATED ITS DNA TO BECOME 4N AND HAS ENTERED INTO THE FIRST PHASE OF MEIOSIS BUT NOT YET DIVIDED, SO THAT IT IS A DIPLOID 4N CELL, WHICH IS CALLED A PRIMARY (1 ) SPERMATOCYTE.

Mitosis Meiosis Meiotic Metaphase I base

blue stain 19680 http://viewer.serenusview.

6 Mitosis Meiosis Meiotic Metaphase I base Mitotic metaphase Mitotic metaphase Meiotic Metaphase II Meiotic metaphase I Secondary spermatocytes lumen Human testis Epon toluidine blue stain e0ced c4-f9239ff75af7

7 SEMINIFEROUS TUBULES COMPOSED OF: SPERMATIDS Primary SPERMATOCYTES SERTOLI CELLS MYOID CELL nucleus GERM CELLS SPERMATOGONIA, SPERMATOCYTES, SPERMATIDS Human testis

8 human

9 Slide #107 (F a). Testis, epididymis, ductus deferens (tail of epididymis), cat. seminiferous tubules rete testis and mediastinal testis Testis epididymis Leydig cells produce TESTOSTERONE interstitial tissue between the seminiferous tubules is quite thin. ductus deferens (tail of epididymis)

10 Slide #107 (F a). Testis, epididymis, ductus deferens (tail of epididymis), cat. CCT capsule s proper TUNICA ALBUGINIA Leydig cells produce TESTOSTERONE The interstitial tissue between the seminiferous tubules is quite thin

Slide #107 (F3-405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.

11 Slide #107 (F a). Testis, epididymis, ductus deferens (tail of epididymis), cat. Sertoli cells; spermatogonia; primary spermatocytes; and early and late spermatids spermatogonia; Secondary spermatocytes

12 Golgi phase Cap phase Elongation phase Maturation phase

sustentacular ( Sertoli ) cells; LEYDIG (INTERSTITIAL) CELLS ARE LARGE, ROUND TO OVAL

13 DEMO SLIDE BOX 100 Testis, stallion. Sertoli cells; spermatogonia; primary spermatocytes; late spermatids Leydig cells sustentacular ( Sertoli ) cells; LEYDIG (INTERSTITIAL) CELLS ARE LARGE, ROUND TO OVAL CELLS WITH ACIDOPHILIC BUT OFTEN PALELY STAINING CYTOPLASM. SOMETIMES THESE CELLS CAN APPEAR FOAMY DUE TO THE FACT THAT THEY ARE STEROID PRODUCING CELLS.

14 DEMO SLIDE BOX 99 Testis, newborn kitten. Nuclei of sustentacular cells epididymis rete testis and mediastinal testis gonocytes

15 DEMO SLIDE BOX 226 (PM-172 b). Testis, epididymis, ductus deferens, pig (immature). epidermidis rete testis and mediastinal testis Dividine gonocytes gonocytes Nuclei of sustentacular ( Sertoli ) cells;

tall Sertoli cells where germ cells would have been located as indicated in the drawing of Sertoli cells Slide

16 DEMO SLIDE BOX 187 (935). Testis, boar. DEGENERATION and loss of germ cells in the seminiferous tubules; however, it illustrates the open spaces between tall Sertoli cells where germ cells would have been located as indicated in the drawing of Sertoli cells Slide #107 Normal cat testis IF THE MALE IS A BILATERAL CRYPTORCHID THEN HE WILL BE INFERTILE; IF THE MALE IS A UNILATERAL CRYPTORCHID THEN HE likely WILL HAVE REDUCED FERTILITY. Cryptorchid testis

17 DEMO SLIDE BOX 189 (985) -Epididymis, goat. ductuli efferentes. ductuli aberrantes vestigial mesonephric tubules ductus epididymidis.

18 DEMO SLIDE BOX 224 (641). Epididymis, boar. ductuli efferentes ductus epididymidis,

19 DEMO SLIDE BOX 101 Ductus deferens, stallion. thick tunica muscularis epithelium and lamina propria/submucosa smooth muscle

20 Slide #119 (Ed c). Ampulla, donkey. proteinaceous material In lumen of the ampulla. CCT and smooth muscle. simple or stratified cuboidal epithelium

21 DEMO SLIDE BOX 102 (998.) Ampulla of ductus deferens, stallion. simple or stratified cuboidal epithelium Smooth muscle

22 Slide #126 (Dog ). Prostate, dog. urethral lumen. TRANSITIONAL simple cuboidal or simple columnar (like the prostate) and doesn t have stereocilia.

23 DEMO SLIDE BOX 105 Prostate gland, dog. fibromuscular tissue Urethra with transitional epithelium small ampullae of the ductus deferens

24 DEMO SLIDE BOX 103 Prostate gland (pars disseminata), bull principal duct of the vesicular glands or ductus deferens; urethra SKELETAL MUSCLE, WHICH IS CALLED THE URETHRALIS MUSCLE OR THE EXTERNAL URETHRAL SPHINCTER

25 Slide #131 (946) -- Bulbourethral gland, boar.

26 Slide #137 (Ed B). Vesicular gland, stallion. seminal vesicles because they are hollow, blind sacs vesicular glands or seminal vesicles

27 DEMO SLIDE BOX 191 (991) Bulbourethral gland, goat. mucous character skeletal muscle fibers of the bulbospongiosus muscle.

(of the penis CCT) urethra -TRANSITIONAL PRIMARILY SMOOTH MUSCLE ( YOU MAY SEE A

28 DEMO SLIDE BOXES 107 Penis, dog. pectiniform septum - separates the paired corpora cavernosum tunica albuginea (of the penis CCT) urethra -TRANSITIONAL PRIMARILY SMOOTH MUSCLE ( YOU MAY SEE A FEW SKELETAL MUSCL FIBERS MIXED IN WITH THE SMOOTH MUSCLE ESPECIALLY PROXIMALLY dorsal penile artery, vein & nerve- MUSCULAR ARTERY

29 DEMO SLIDE BOXES 108 Penis, dog.

30 DEMO SLIDE 193 (1086). Penis, dog. the glans penis and includes part of the prepuce internal lamina of the prepuce preputial cavity skin of the penis pars longa glandis - peripherally located erectile tissue urethra corpus spongiosum - erectile tissue surrounding the urethra os penis

31 DEMO SLIDE BOX 192 (1054). Penis, bull calf. corpus cavernosum penis, urethra, corpus spongiosum. skin of the penis and internal lamina of the prepuce are fused at this location

32 Slide #56 (Pm1-166C-B1). Penis, boar (immature). corpus cavernosum penis, the urethra, and the corpus spongiosum. nerve, collagenous connective tissue (CCT), large vein, arterioles, venules, capillaries. nerve Small muscular artery, Unilocular adipose Tissue (white fat),

33 277 Human Penis transitional epithelium and surrounding spongy cavernous of penal urethra corpus cavernosum penis urethra nerve Transitional epithelium of urethra skin Corpus spongiosum Tunica albuginea (of the penis, CCT) Smooth muscle Corpus spongiosum

MALE REPRODUCTIVE SYSTEM

MALE REPRODUCTIVE SYSTEM The male reproductive system consists of primary sex organs (testes) and secondary or accessory sex organs. The secondary organs consist of a series of genital ducts (ductules