CD86 (B7-2) Monoclonal Antibody (PO3.1), PE, ebioscience Catalog Number Product data sheet

Transcription

1 Website: thermofisher.com/ebioscience Customer Service (US): thermofisher.com/contactus CD86 (B7-2) Monoclonal Antibody (PO3.1), PE, ebioscience Catalog Number Product data sheet Details Size 100 µg Host/Isotope Class Type Clone Conjugate Form Concentration Purification Storage buffer Contains Storage Conditions Rat / IgG2b, kappa Monoclonal Antibody PO3.1 PE Liquid 0.2 mg/ml Affinity chromatography PBS, ph 7.2, with 0.1% gelatin 0.09% sodium azide 4 C, store in dark, DO NOT FREEZE! Species Reactivity Species reactivity Mouse Published species Mouse, Not Applicable Tested Applications Dilution * Flow Cytometry (Flow) µg/test Published Applications Flow Cytometry (Flow) See 18 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information Description: The PO3.1 monoclonal antibody reacts with mouse CD86, an ~80 kda surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response. Applications Reported: The PO3.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: The PO3.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 10^5 to 10^8 cells /test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Background/Target Information CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

2 Product Images For CD86 (B7-2) Monoclonal Antibody (PO3.1), PE, ebioscience CD86 (B7-2) Antibody ( ) in Flow Surface staining of LPS-stimulated splenocytes with Rat IgG2b kappa Isotype Control PE (Product # ) (open histogram) and Anti-Mouse CD86 (B7-2) PE (filled histogram). Total viable cells were used for analysis.

3 PubMed References For CD86 (B7-2) Monoclonal Antibody (PO3.1), PE, ebioscience 18 Flow Cytometry References Species / Dilution Summary was used in flow cytometry to study cancer-testis antigen expression in a mouse model of prostate cancer The Prostate (Mar 2017; 77: 361) "Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model." Author(s):Sulek JE,Robinson SP,Petrossian AA,Zhou S,Goliadze E,Manjili MH,Toor A,Guruli G PubMed Article URL: was used in Flow cytometry/cell sorting to evaluate the effects of immunisation with a synthetic hexasaccharide conjugate, with and without an adjuvant, on TLR activation, dendritic cell maturation, and cytokine production in mice. Frontiers in immunology (Jul 2016; 7: null) "The Effect of a BSA Conjugate of a Synthetic Hexasaccharide Related to the Fragment of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 on the Activation of Innate and Adaptive Immune Responses." Author(s):Akhmatova NK,Kurbatova EA,Akhmatov EA,Egorova NB,Logunov DY,Gening ML,Sukhova EV,Yashunsky DV, Tsvetkov YE,Nifantiev NE PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the effect of BCG-TB1860 on both the adaptive and innate immune responses. PLoS pathogens (Jun 2014; 10: null) "The glycosylated Rv1860 protein of Mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG." Author(s):Satchidanandam V,Kumar N,Jumani RS,Challu V,Elangovan S,Khan NA PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate that immune modulation by calcitriol may be a potentially valuable therapeutic approach against atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology (Dec 2010; 30: 2495) "Oral administration of an active form of vitamin D3 (calcitriol) decreases atherosclerosis in mice by inducing regulatory T cells and immature dendritic cells with tolerogenic functions." Author(s):Takeda M,Yamashita T,Sasaki N,Nakajima K,Kita T,Shinohara M,Ishida T,Hirata K PubMed Article URL: was used in Flow cytometry/cell sorting to evaluate the capacity of mesenchymal stromal cell-derived extracellular vesicles to rescue murine marrow hematopoietic cells damage following exposure to radiation, showing that damage could be reversed using both murine and human MSC-EVs. Leukemia (Nov 2016; 30: 2221) "Mesenchymal stromal cell-derived extracellular vesicles rescue radiation damage to murine marrow hematopoietic cells." Author(s):Wen S,Dooner M,Cheng Y,Papa E,Del Tatto M,Pereira M,Deng Y,Goldberg L,Aliotta J,Chatterjee D,Stewart C, Carpanetto A,Collino F,Bruno S,Camussi G,Quesenberry P PubMed Article URL: was used in Flow cytometry/cell sorting to examine the phenotype of TSLP-conditioned bone marrow dendritic cells of apolipoprotein E-deficient mice and their capacity to induce the differentiation of Tregs. Journal of the American Heart Association (Aug 2013; 2: null) "Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice." Author(s):Yu K,Zhu P,Dong Q,Zhong Y,Zhu Z,Lin Y,Huang Y,Meng K,Ji Q,Yi G,Zhang W,Wu B,Mao Y,Cheng P,Zhao X,Mao X,Zeng Q PubMed Article URL: was used in Flow cytometry/cell sorting to create a model to investigate the mechanisms involved in chronic uremiaassociated immunodeficiency and immunization of chronic kidney disease patient problems. Comparative medicine (Aug 2015; 65: 308) "Effect of Chronic Uremia on the Cell Surface Expression of B7 Family Costimulatory Molecules in an HLA-A2 Transgenic Mouse Model of Chronic Kidney Disease." Author(s):Makidon PE,Smith DM,Groom Ii JV,Cao Z,Landers JJ,Baker JR PubMed Article URL:

4 was used in Flow cytometry/cell sorting to investigate how type-2 immunity is affected by STAT5 transcription factor in dendritic cells. Nature immunology (Apr 2013; 14: 364) "The transcription factor STAT5 is critical in dendritic cells for the development of TH2 but not TH1 responses." Author(s):Bell BD,Kitajima M,Larson RP,Stoklasek TA,Dang K,Sakamoto K,Wagner KU,Kaplan DH,Reizis B,Hennighausen L, Ziegler SF PubMed Article URL: was used in Flow cytometry/cell sorting to develop a mouse model of debridement to evaluate its effectiveness in the treatment of Bacillus anthracis soft tissue infections. PloS one (Jul 2012; 7: null) "Debridement increases survival in a mouse model of subcutaneous anthrax." Author(s):Weiner ZP,Boyer AE,Gallegos-Candela M,Cardani AN,Barr JR,Glomski IJ PubMed Article URL: was used in Flow cytometry to evaluate the targeting of vaccine antigen to dendritic cells via Clec9A for efficient induction of cellular immunity. Journal of immunology (Baltimore, Md. : 1950) (Jul 2011; 187: 842) "Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype." Author(s):Lahoud MH,Ahmet F,Kitsoulis S,Wan SS,Vremec D,Lee CN,Phipson B,Shi W,Smyth GK,Lew AM,Kato Y,Mueller SN,Davey GM,Heath WR,Shortman K,Caminschi I PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate that location of tumour, and not simply the tumour itself, has a definitive role in regulating immune effectors. Immunity, inflammation and disease (Mar 2017; 5: 85) "Location of tumor affects local and distant immune cell type and number." Author(s):Hensel JA,Khattar V,Ashton R,Lee C,Siegal GP,Ponnazhagan S PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the effect of embryonic stem cell-derived dendritic cells on autoimmunity, showing that suppression of Th1-mediated autoimmunity occurs in NOD and EAE mice. PloS one (Dec 2015; 9: null) "Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells." Author(s):Ikeda T,Hirata S,Takamatsu K,Haruta M,Tsukamoto H,Ito T,Uchino M,Ando Y,Nagafuchi S,Nishimura Y,Senju S PubMed Article URL: was used in Flow cytometry/cell sorting to investigate whether human ipscs (hipscs) have the ability to enhance specific immune responses against a HIV-1 antigen in a xenogenic mouse model. Frontiers in microbiology (Jul 2011; 2: null) "Vaccination with Human Induced Pluripotent Stem Cells Creates an Antigen-Specific Immune Response Against HIV-1 gp160." Author(s):Yoshizaki S,Nishi M,Kondo A,Kojima Y,Yamamoto N,Ryo A PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate a role for TSLP in a Th2 model of contact hypersensitivity in mice. Journal of immunology (Baltimore, Md. : 1950) (Mar 2010; 184: 2974) "Dibutyl phthalate-induced thymic stromal lymphopoietin is required for Th2 contact hypersensitivity responses." Author(s):Larson RP,Zimmerli SC,Comeau MR,Itano A,Omori M,Iseki M,Hauser C,Ziegler SF PubMed Article URL: was used in Flow cytometry/cell sorting to suggest that La parasites, especially in their intracellular forms, have evolved unique strategies to actively down-regulate early innate signalling events. Molecular immunology (Jul 2008; 45: 3371) "Down-regulation of dendritic cell signaling pathways by Leishmania amazonensis amastigotes." Author(s):Xin L,Li K,Soong L PubMed Article URL:

5 was used in Flow cytometry/cell sorting to investigate the effect of the administration of EPA on the regression of atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology (Sep 2011; 31: 1963) "Orally administered eicosapentaenoic acid induces rapid regression of atherosclerosis via modulating the phenotype of dendritic cells in LDL receptor-deficient mice." Author(s):Nakajima K,Yamashita T,Kita T,Takeda M,Sasaki N,Kasahara K,Shinohara M,Rikitake Y,Ishida T,Yokoyama M, Hirata K PubMed Article URL: was used in Flow cytometry/cell sorting to analyse the cellular expression pattern and functional properties of Siglec- H. Blood (May 2006; 107: 3600) "Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors." Author(s):Zhang J,Raper A,Sugita N,Hingorani R,Salio M,Palmowski MJ,Cerundolo V,Crocker PR PubMed Article URL: was used in Flow cytometry/cell sorting to determine that although mesenchymal stem cells possess immunosuppressive properties, they may not be immunoprivileged. Journal of tissue engineering (May 2014; 5: null) "Immunogenicity of undifferentiated and differentiated allogeneic mouse mesenchymal stem cells." Author(s):Mukonoweshuro B,Brown CJ,Fisher J,Ingham E PubMed Article URL: