CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE, ebioscience Catalog Number Product data sheet

Transcription

1 Website: thermofisher.com/ebioscience Customer Service (US): thermofisher.com/contactus CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE, ebioscience Catalog Number Product data sheet Details Size 100 µg Host/Isotope Class Type Clone Conjugate Form Concentration Purification Storage buffer Contains Storage Conditions Rat / IgG2a, lambda Monoclonal Antibody MIH5 PE Liquid 0.2 mg/ml Affinity chromatography PBS, ph 7.2, with 0.1% gelatin 0.09% sodium azide 4 C, store in dark, DO NOT FREEZE! Species Reactivity Tested species reactivity Mouse Published species reactivity Mouse, Not Applicable Tested Applications Dilution * Flow Cytometry (Flow) 0.06 µg/test Published Applications Flow Cytometry (Flow) See 22 publications below Neutralization (Neu) See 1 publications below Functional Assay (FN) See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kda and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody. Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis. Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Background/Target Information Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

2 Product Images For CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE, ebioscience CD274 (PD-L1, B7- H1) Antibody ( ) in Flow Staining of C57Bl/6 splenocytes with Anti- Mouse CD3e APC (Product # ) and 0.03 µg of Rat IgG2a K Isotype Control PE (Product # ) (left) or 0.03 µg of Anti-Mouse CD274 (B7-H1) PE (right). Cells in the lymphocyte gate were used for analysis.

3 PubMed References For CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE, ebioscience 22 Flow Cytometry References was used in Flow cytometry/cell sorting to determine whether loss of Stard7 expression promotes allergic responses, showing that haploinsufficiency for Stard7 is associated with enhanced allergic responses. Mouse / 1:100 Journal of immunology (Baltimore, Md. : 1950) (Jun 2015; 194: 5635) "Haploinsufficiency for Stard7 is associated with enhanced allergic responses in lung and skin." Author(s):Yang L,Lewkowich I,Apsley K,Fritz JM,Wills-Karp M,Weaver TE PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the role of the brain in innate immunological signals, showing rapid linkage of innate signals and adaptive immunity by the brain-fat axis. Nature immunology (May 2015; 16: 525) "Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis." Author(s):Kim MS,Yan J,Wu W,Zhang G,Zhang Y,Cai D PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the role of GF-3-expressing CD4+CD25(-)LAG3+ Tregs in humoral immune responses. Nature communications (Feb 2015; 6: null) "TGF-3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses." Author(s):Okamura T,Sumitomo S,Morita K,Iwasaki Y,Inoue M,Nakachi S,Komai T,Shoda H,Miyazaki J,Fujio K,Yamamoto K PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the mechanisms by which HIV-1 induces upregulation of coinhibitory molecules, showing that the HIV-1 Tat protein induces PD-L1 expression on dendritic cells. Journal of virology (Jun 2014; 88: 6672) "HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and tolllike receptor 4-mediated mechanisms." Author(s):Planès R,BenMohamed L,Leghmari K,Delobel P,Izopet J,Bahraoui E PubMed Article URL: was used in Flow cytometry/cell sorting to reveal a novel role for oxidised lipids in the regulation of crosspresentation. Journal of immunology (Baltimore, Md. : 1950) (Mar 2014; 192: 2920) "Oxidized lipids block antigen cross-presentation by dendritic cells in cancer." Author(s):Ramakrishnan R,Tyurin VA,Tuyrin VA,Veglia F,Condamine T,Amoscato A,Mohammadyani D,Johnson JJ,Zhang LM, Klein-Seetharaman J,Celis E,Kagan VE,Gabrilovich DI PubMed Article URL: was used in Flow cytometry/cell sorting to study Kupffer cell-mediated potentiation of liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1. Journal of leukocyte biology (Nov 2013; 94: 963) "Kupffer cells potentiate liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1." Author(s):Hutchins NA,Wang F,Wang Y,Chung CS,Ayala A PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate a novel function by which alternatively activated macrophages control large extracellular parasites. Infection and immunity (Sep 2013; 81: 3346) "Human and mouse macrophages collaborate with neutrophils to kill larval Strongyloides stercoralis." Author(s):Bonne-Année S,Kerepesi LA,Hess JA,O'Connell AE,Lok JB,Nolan TJ,Abraham D PubMed Article URL: was used in Flow cytometry/cell sorting to examine the phenotype of TSLP-conditioned bone marrow dendritic cells of apolipoprotein E-deficient mice and their capacity to induce the differentiation of Tregs. Journal of the American Heart Association (Aug 2013; 2: null) "Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice." Author(s):Yu K,Zhu P,Dong Q,Zhong Y,Zhu Z,Lin Y,Huang Y,Meng K,Ji Q,Yi G,Zhang W,Wu B,Mao Y,Cheng P,Zhao X,Mao X,Zeng Q PubMed Article URL:

4 was used in Flow cytometry/cell sorting to demonstrate that PD-L1 knockdown on KCs is central in determining the outcome of liver viral infections, and they represent a new class of gene therapy. Molecular therapy. Nucleic acids (Feb 2013; 2: null) "Lipidoid Nanoparticles Containing PD-L1 sirna Delivered In Vivo Enter Kupffer Cells and Enhance NK and CD8(+) T Cell-mediated Hepatic Antiviral Immunity." Author(s):Dolina JS,Sung SS,Novobrantseva TI,Nguyen TM,Hahn YS PubMed Article URL: was used in Flow cytometry/cell sorting to provide new insights into the development of sirna-based therapeutics for the treatment of T1D. Molecular therapy : the journal of the American Society of Gene Therapy (Dec 2012; 20: 2315) "Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes." Author(s):Leconet W,Petit P,Peraldi-Roux S,Bresson D PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the molecular mechanisms of PD-1-mediated suppression of T cell activation, showing that it inhibits T cell receptor signalling via SHP2. The Journal of experimental medicine (Jun 2012; 209: 1201) "Programmed cell death 1 forms negative costimulatory microclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2." Author(s):Yokosuka T,Takamatsu M,Kobayashi-Imanishi W,Hashimoto-Tane A,Azuma M,Saito T PubMed Article URL: was used in Flow cytometry/cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules. European journal of microbiology and immunology (Jun 2012; 2: 112) "Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function." Author(s):Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D PubMed Article URL: was used in Flow cytometry/cell sorting to study the regulation of CD4 T cell cytokine release during viral lung disease, by OX40 and PD-L2 expression on inflammatory dendritic cells. Journal of immunology (Baltimore, Md. : 1950) (Feb 2012; 188: 1647) "OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease." Author(s):Wythe SE,Dodd JS,Openshaw PJ,Schwarze J PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the function and mechanism of action of PD-L1 in experimental sepsis in mice. Critical care (London, England) (Mar 2011; 14: null) "PD-L1 blockade improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction." Author(s):Zhang Y,Zhou Y,Lou J,Li J,Bo L,Zhu K,Wan X,Deng X,Cai Z PubMed Article URL: was used in Flow cytometry/cell sorting to suggest that blockade of the PD-1/PD-L1 is not likely to be efficient at restoring exhausted T-cell responses in aged hosts. Aging cell (Oct 2010; 9: 785) "Partial restoration of T-cell function in aged mice by in vitro blockade of the PD-1/ PD-L1 pathway." Author(s):Lages CS,Lewkowich I,Sproles A,Wills-Karp M,Chougnet C PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the mechanisms by which the retina protects itself from CD4 T- cell-mediated immune damage in retinal immune responses. Infection and immunity (Aug 2010; 78: 3484) "CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1." Author(s):Charles E,Joshi S,Ash JD,Fox BA,Farris AD,Bzik DJ,Lang ML,Blader IJ PubMed Article URL:

5 was used in Flow cytometry/cell sorting to investigate CD11b(+)Gr-1(+) cells found in epithelial ovarian cancerbearing mice, which are induced by ovarian cancer cells and undergo antigen-specific immunity and cross-priming. Not Applicable / Not Cited 1 Neutralization References Journal of immunology (Baltimore, Md. : 1950) (Jun 2010; 184: 6151) "Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells." Author(s):Tomihara K,Guo M,Shin T,Sun X,Ludwig SM,Brumlik MJ,Zhang B,Curiel TJ,Shin T PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the roles of PD-L1 and PD-L2 in regulating invariant natural killer T cell mediated airway hyperreactivity in a mouse model of asthma. Mucosal immunology (Jan 2010; 3: 81) "PD-L1 and PD-L2 modulate airway inflammation and inkt-cell-dependent airway hyperreactivity in opposing directions." Author(s):Akbari O,Stock P,Singh AK,Lombardi V,Lee WL,Freeman GJ,Sharpe AH,Umetsu DT,Dekruyff RH PubMed Article URL: was used in Flow cytometry/cell sorting to study the dynamics of lung macrophage activation in response to helminth infection. Journal of leukocyte biology (Dec 2008; 84: 1422) "Dynamics of lung macrophage activation in response to helminth infection." Author(s):Siracusa MC,Reece JJ,Urban JF,Scott AL PubMed Article URL: Cell research (Aug 2008; 18: 846) "A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1." Author(s):Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N PubMed Article URL: was used in Flow cytometry/cell sorting to identify 2 distinct MDSC subfractions with clear morphologic, molecular, and functional differences. Blood (Apr 2008; 111: 4233) "Identification of discrete tumor-induced myeloid-derived suppressor cell subpopulations with distinct T cellsuppressive activity." Author(s):Movahedi K,Guilliams M,Van den Bossche J,Van den Bergh R,Gysemans C,Beschin A,De Baetselier P,Van Ginderachter JA PubMed Article URL: was used in Flow cytometry/cell sorting to study the blockade of PD-1/B7-H1 interaction to restore effector CD8+ T cell responses in a hepatitis C virus core murine model. Journal of immunology (Baltimore, Md. : 1950) (Apr 2008; 180: 4875) "Blockade of PD-1/B7-H1 interaction restores effector CD8+ T cell responses in a hepatitis C virus core murine model." Author(s):Lukens JR,Cruise MW,Lassen MG,Hahn YS PubMed Article URL: was used in Blocking experiments to investigate the role of B7-H1 in the conversion of normal cells to tumour cells. 1 Functional Assay References Not Applicable / Not Cited Cancer research (Feb 2011; 71: 1235) "B7-H1 overexpression regulates epithelial-mesenchymal transition and accelerates carcinogenesis in skin." Author(s):Cao Y,Zhang L,Kamimura Y,Ritprajak P,Hashiguchi M,Hirose S,Azuma M PubMed Article URL: Blood (Mar 2005; 105: 2428) "NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells." Author(s):Saudemont A,Jouy N,Hetuin D,Quesnel B PubMed Article URL: