CD86 (B7-2) Monoclonal Antibody (IT2.2), PE, ebioscience Catalog Number Product data sheet

Transcription

1 Website: thermofisher.com/ebioscience Customer Service (US): thermofisher.com/contactus CD86 (B7-2) Monoclonal Antibody (IT2.2), PE, ebioscience Catalog Number Product data sheet Details Size Host/Isotope Class Type Clone Conjugate Form Concentration Purification Storage buffer Contains Storage Conditions 25 Tests Mouse / IgG2b, kappa Monoclonal Antibody IT2.2 PE Liquid 5 µl/test Affinity chromatography PBS, ph 7.2, with 0.1% gelatin, 0.2% BSA 0.09% sodium azide 4 C, store in dark, DO NOT FREEZE! Species Reactivity Species reactivity Published species Human Tested Applications Dilution * Flow Cytometry (Flow) Published Applications Flow Cytometry (Flow) Immunocytochemistry (ICC) Immunofluorescence (IF) Hamster, Human, Mouse, Not Applicable 5 µl (0.5 µg)/test See 18 publications below See 1 publications below See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kda surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response. Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µl (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Background/Target Information CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

2 Product Images For CD86 (B7-2) Monoclonal Antibody (IT2.2), PE, ebioscience CD86 (B7-2) Antibody ( ) in Flow Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control PE (Product # ) (blue histogram) or Anti-Human CD86 (B7-2) PE (purple histogram). Cells in the monocyte gate were used for analysis.

3 PubMed References For CD86 (B7-2) Monoclonal Antibody (IT2.2), PE, ebioscience 18 Flow Cytometry References was used in Flow cytometry/cell sorting to determine the mechanisms behind the activation of monocytes by reverse signalling through the CD137 ligand. Human / 1:200 FASEB journal : official publication of the Federation of American Societies for Experimental Biology (Aug 2013; 27: 2957) "Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling." Author(s):Moh MC,Lorenzini PA,Gullo C,Schwarz H PubMed Article URL: was used in Flow cytometry/cell sorting to assess the role of TLR6 in the immune status of umbilical cord mesenchymal stem cells, by stimulation with the TLR6 agonist MALP-2. Experimental and therapeutic medicine (Dec 2017; 14: 5540) "MALP-2, an agonist of TLR6, promotes the immune status without affecting the differentiation capacity of umbilical cord mesenchymal stem cells." Author(s):Wu X,Xu L,Shen Y,Yu N,Zhang Y,Guo T PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients. BioMed research international (Jul 2015; 2014: null) "Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients." Author(s):Lopes DM,Fernandes JS,Cardoso TM,Bafica AM,Oliveira SC,Carvalho EM,Araujo MI,Cardoso LS PubMed Article URL: was used in Flow cytometry/cell sorting to show that monocytes stimulated with Brugia malayi microfilarial lysate develop a defined regulatory phenotype. PLoS neglected tropical diseases (Oct 2014; 8: null) "Brugia malayi microfilariae induce a regulatory monocyte/macrophage phenotype that suppresses innate and adaptive immune responses." Author(s):O'Regan NL,Steinfelder S,Venugopal G,Rao GB,Lucius R,Srikantam A,Hartmann S PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate that PSGL-1 present on the cell surfaces of monocytederived dendritic cells mediates enterovirus 71 binding and facilitates further infection. Virulence (Oct 2016; 6: 802) "Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death." Author(s):Ren XX,Li C,Xiong SD,Huang Z,Wang JH,Wang HB PubMed Article URL: was used in Flow cytometry/cell sorting to explore the use of oligonucleotide sequences for therapeutic purposes in situations where inflammation is enhanced by TLR9. International immunology (Mar 2011; 23: 203) "Optimal oligonucleotide sequences for TLR9 inhibitory activity in human cells: lack of correlation with TLR9 binding." Author(s):Ashman RF,Goeken JA,Latz E,Lenert P PubMed Article URL: was used in Flow cytometry/cell sorting to examine whether the interaction of genetically modified Francisella tularensis live vaccine strains with human antigen-presenting cells correlates with vaccine effectiveness. PloS one (Jun 2012; 7: null) "A Francisella tularensis live vaccine strain that improves stimulation of antigen-presenting cells does not enhance vaccine efficacy." Author(s):Schmitt DM,O'Dee DM,Horzempa J,Carlson PE,Russo BC,Bales JM,Brown MJ,Nau GJ PubMed Article URL:

4 was used in Flow cytometry/cell sorting to investigate whether the immunoregulatory receptors immunoglobulin-like transcript 3 and 4 mediate the therapeutic effect of interferon beta in multiple sclerosis. Mouse / Not Cited Human / 1:100 Mouse / Not Cited PloS one (Sep 2015; 9: null) "Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients." Author(s):Waschbisch A,Sanderson N,Krumbholz M,Vlad G,Theil D,Schwab S,Mäurer M,Derfuss T PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the the possible role of vitamin D deficiency as a mechanism of increased atherosclerosis in T2DM patients. The Journal of biological chemistry (Nov 2012; 287: 38482) "Vitamin D suppression of endoplasmic reticulum stress promotes an antiatherogenic monocyte/macrophage phenotype in type 2 diabetic patients." Author(s):Riek AE,Oh J,Sprague JE,Timpson A,de las Fuentes L,Bernal-Mizrachi L,Schechtman KB,Bernal-Mizrachi C PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the consequences of inhibiting lysine-specific demethylase 1 in a panel of cell lines representing all acute myelogenous leukaemia subtypes. Cancer research (Apr 2016; 76: 1975) "Pharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes." Author(s):McGrath JP,Williamson KE,Balasubramanian S,Odate S,Arora S,Hatton C,Edwards TM,O'Brien T,Magnuson S, Stokoe D,Daniels DL,Bryant BM,Trojer P PubMed Article URL: was used in Flow cytometry/cell sorting to describe Leishmania major infection in humanised mice for the first time, characterising disease development and the induction of the human immune response. PLoS neglected tropical diseases (Nov 2012; 6: null) "Leishmania major infection in humanized mice induces systemic infection and provokes a nonprotective human immune response." Author(s):Wege AK,Florian C,Ernst W,Zimara N,Schleicher U,Hanses F,Schmid M,Ritter U PubMed Article URL: was used in Flow cytometry/cell sorting to describe a novel platform that enables the interrogation and screening of APC responses to TLR ligands. Journal of immunological methods (Jul 2008; 336: 183) "Polychromatic flow cytometric high-throughput assay to analyze the innate immune response to Toll-like receptor stimulation." Author(s):Jansen K,Blimkie D,Furlong J,Hajjar A,Rein-Weston A,Crabtree J,Reikie B,Wilson C,Kollmann T PubMed Article URL: was used in Flow cytometry/cell sorting to study the role of CX3CR1+ mononuclear phagocytes in the development of colitis and disease progression following Citrobacter rodentium infection. The Journal of experimental medicine (Jul 2014; 211: 1571) "CXCR1 mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22." Author(s):Longman RS,Diehl GE,Victorio DA,Huh JR,Galan C,Miraldi ER,Swaminath A,Bonneau R,Scherl EJ,Littman DR PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate that DC interaction with HIV-1 has been exploited by P. marneffei for viral amplification. PloS one (Mar 2012; 6: null) "Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells." Author(s):Qin Y,Li Y,Liu W,Tian R,Guo Q,Li S,Li H,Zhang D,Zheng Y,Wu L,Lan K,Wang J PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the functional interplay between M-CSF and IL-32 acting on macrophages. Journal of immunology (Baltimore, Md. : 1950) (Jun 2014; 192: 5083) "M-CSF inhibits anti-hiv-1 activity of IL-32, but they enhance M2-like phenotypes of macrophages." Author(s):Osman A,Bhuyan F,Hashimoto M,Nasser H,Maekawa T,Suzu S PubMed Article URL:

5 was used in Flow cytometry/cell sorting to determine that dendritic cells may mediate or regulate skin inflammation by releasing PGD2 in response to various stimuli. The American journal of pathology (Jan 2010; 176: 227) "Dendritic cells express hematopoietic prostaglandin D synthase and function as a source of prostaglandin D2 in the skin." Author(s):Shimura C,Satoh T,Igawa K,Aritake K,Urade Y,Nakamura M,Yokozeki H PubMed Article URL: was used in Flow cytometry/cell sorting to prove CTLLA-4-antigen fusion enhances anti-caries DNA vaccines. Hamster / Not Cited 1 Immunocytochemistry References Human / 1:5 Acta pharmacologica Sinica (Aug 2007; 28: 1236) "Enhanced efficacy of CTLA-4 fusion anti-caries DNA vaccines in gnotobiotic hamsters." Author(s):Zhang F,Li YH,Fan MW,Jia R,Xu QA,Guo JH,Yu F,Tian QW PubMed Article URL: was used in Flow cytometry/cell sorting to suggest that CD80 and CD86 activate T cells in IgAN, CD80/CD86 expressions correlated with renal function at the time of renal biopsy, and monocyte/macrophages and tubular epithelial cells act as APC. Kidney international (Mar 2004; 65: 888) "Clinical significance of costimulatory molecules CD80/CD86 expression in IgA nephropathy." Author(s):Wu Q,Jinde K,Endoh M,Sakai H PubMed Article URL: 1 Immunofluorescence References was used in Immunocytochemistry to investigate the function of pentraxin-3 in macrophages in vitro. Biochemistry and biophysics reports (Mar 2016; 5: 290) "Pentraxin-3 regulates the inflammatory activity of macrophages." Author(s):Shiraki A,Kotooka N,Komoda H,Hirase T,Oyama JI,Node K PubMed Article URL: was used in Immunocytochemistry to investigate the function of pentraxin-3 in macrophages in vitro. Human / 1:5 Biochemistry and biophysics reports (Mar 2016; 5: 290) "Pentraxin-3 regulates the inflammatory activity of macrophages." Author(s):Shiraki A,Kotooka N,Komoda H,Hirase T,Oyama JI,Node K PubMed Article URL: