CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE, ebioscience Catalog Number Product data sheet

Transcription

1 Website: thermofisher.com/ebioscience Customer Service (US): thermofisher.com/contactus CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE, ebioscience Catalog Number Product data sheet Details Size 50 µg Host/Isotope Class Type Clone Conjugate Form Concentration Purification Storage buffer Contains Storage Conditions Mouse / IgG2a, kappa Monoclonal Antibody HIS51 PE Liquid 0.2 mg/ml Affinity chromatography PBS, ph 7.2, with 0.1% gelatin 0.09% sodium azide 4 C, store in dark, DO NOT FREEZE! Species Reactivity Species reactivity Mouse, Rat Published species Rat, Mouse, Human, Not Applicable Tested Applications Dilution * Flow Cytometry (Flow) 0.06 µg/test Published Applications Flow Cytometry (Flow) See 18 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells. Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Background/Target Information CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

2 Product Images For CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE, ebioscience CD90.1 (Thy-1.1) Antibody ( ) in Flow Staining of rat splenocytes with Mouse IgG2a kappa Isotype Control FITC (Product # ) (left, open histogram) or Mouse IgG2a kappa Isotype Control PE (Product # ) (right, open histogram) and Anti-Mouse/Rat CD90.1 (Thy- 1.1) FITC (Product # ) (left, filled histogram) or 0.03 µg of Anti-Mouse/Rat CD90.1 (Thy-1.1) PE (right, filled histogram). Total viable cells were used for analysis.

3 PubMed References For CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE, ebioscience 18 Flow Cytometry References Species / Dilution Summary was used in Flow cytometry/cell sorting to demonstrate that basophils play an important role for protective immunity against helminths and orchestrate chronic allergic inflammation. Rat / 1:1000 Immunity (Sep 2010; 33: 364) "Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths." Author(s):Ohnmacht C,Schwartz C,Panzer M,Schiedewitz I,Naumann R,Voehringer D PubMed Article URL: was used in Flow cytometry/cell sorting to define a critical role for the FL/IFN-I/Flt3 axis in plasmacytoid dendritic cell differentiation from common lymphoid progenitors. The Journal of experimental medicine (Nov 2013; 210: 2515) "A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors." Author(s):Chen YL,Chen TT,Pai LM,Wesoly J,Bluyssen HA,Lee CK PubMed Article URL: was used in Flow cytometry/cell sorting to develop efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats. Molecular medicine reports (Jul 2015; 12: 913) "Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats." Author(s):Li YQ,Tang Y,Fu R,Meng QH,Zhou X,Ling ZM,Cheng X,Tian SW,Wang GJ,Liu XG,Zhou LH PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate that Oct-4+ pluripotent stem cells (VSELs) are mobilized from the bone marrow into the peripheral blood after acute myocardial infarction. Journal of molecular and cellular cardiology (May 2008; 44: 865) "Bone marrow-derived pluripotent very small embryonic-like stem cells (VSELs) are mobilized after acute myocardial infarction." Author(s):Zuba-Surma EK,Kucia M,Dawn B,Guo Y,Ratajczak MZ,Bolli R PubMed Article URL: was used in Flow cytometry/cell sorting to show that vitiligo generates self antigen required for mounting persistent and protective memory CD8+ T cell responses to melanoma. The Journal of clinical investigation (May 2011; 121: 1797) "Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma." Author(s):Byrne KT,Côté AL,Zhang P,Steinberg SM,Guo Y,Allie R,Zhang W,Ernstoff MS,Usherwood EJ,Turk MJ PubMed Article URL: was used in Flow cytometry/cell sorting to study how IL-2 function is markedly inhibited by binding to soluble IL- 2Ralpha. Proceedings of the National Academy of Sciences of the United States of America (Jun 2006; 103: 9166) "Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}." Author(s):Rubinstein MP,Kovar M,Purton JF,Cho JH,Boyman O,Surh CD,Sprent J PubMed Article URL: was used in Flow cytometry to identify an essential role of DC cholesterol efflux pathways in maintaining immune tolerance. Cell metabolism (Jun 2017; 25: 1294) "Cholesterol Accumulation in Dendritic Cells Links the Inflammasome to Acquired Immunity." Author(s):Westerterp M,Gautier EL,Ganda A,Molusky MM,Wang W,Fotakis P,Wang N,Randolph GJ,D'Agati VD,Yvan- Charvet L,Tall AR PubMed Article URL:

4 was used in Flow cytometry/cell sorting to demonstrate that ongoing CD8 T-cell-mediated autoimmune vitiligo is a weakly immunogenic event perpetuated by long-lived T-cells. Journal of immunology (Baltimore, Md. : 1950) (Feb 2014; 192: 1433) "Autoimmune vitiligo does not require the ongoing priming of naive CD8 T cells for disease progression or associated protection against melanoma." Author(s):Byrne KT,Zhang P,Steinberg SM,Turk MJ PubMed Article URL: was used in Flow cytometry/cell sorting to study the Notch-Delta interactions that promote T cell development. The Journal of experimental medicine (Feb 2007; 204: 331) "Hierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation." Author(s):Besseyrias V,Fiorini E,Strobl LJ,Zimber-Strobl U,Dumortier A,Koch U,Arcangeli ML,Ezine S,Macdonald HR,Radtke F PubMed Article URL: was used in Flow cytometry/cell sorting to study whether cytotoxic T lymphocytes targeting multiple antigens are more effective for preventing tumour escape than those targeting a single antigen. Human gene therapy (Oct 2012; 23: 1054) "Adoptive transfer of cytotoxic T lymphocytes targeting two different antigens limits antigen loss and tumor escape." Author(s):Kaluza KM,Kottke T,Diaz RM,Rommelfanger D,Thompson J,Vile R PubMed Article URL: was used in Flow cytometry/cell sorting to study the role of DRAK2 in thymocyte selection and lymphoid maturation, and demonstrate that DRAK2 transduces non-apoptotic signals during thymocyte differentiation. International immunology (Nov 2005; 17: 1379) "DRAK2, a lymphoid-enriched DAP kinase, regulates the TCR activation threshold during thymocyte selection." Author(s):Friedrich ML,Wen BG,Bain G,Kee BL,Katayama C,Murre C,Hedrick SM,Walsh CM PubMed Article URL: was used in Flow cytometry/cell sorting to suggest that the presence of anti-vectorial immunity impacts the rate of differentiation of vector-induced naive CD8 T cells. Journal of immunology (Baltimore, Md. : 1950) (Sep 2011; 187: 2923) "The impact of pre-existing memory on differentiation of newly recruited naive CD8 T cells." Author(s):Martin MD,Wirth TC,Lauer P,Harty JT,Badovinac VP PubMed Article URL: was used in Flow cytometry/cell sorting to describe a novel form of homeostatic proliferation that occurs when naive T cells encounter raised levels of IL-2 and IL-15 in vivo. The Journal of experimental medicine (Aug 2007; 204: 1787) "An intense form of homeostatic proliferation of naive CD8+ cells driven by IL-2." Author(s):Cho JH,Boyman O,Kim HO,Hahm B,Rubinstein MP,Ramsey C,Kim DM,Surh CD,Sprent J PubMed Article URL: was used in Flow cytometry/cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis. Cytometry. Part A : the journal of the International Society for Analytical Cytology (Apr 2008; 73: 351) "Characterization of fibroblasts responsible for cartilage destruction in arthritis." Author(s):Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U PubMed Article URL: was used in Flow cytometry/cell sorting to support further use of CD4 T cell depletion therapy for inducing long-lived immunity to cancer. PloS one (Mar 2012; 6: null) "Protective CD8 memory T cell responses to mouse melanoma are generated in the absence of CD4 T cell help." Author(s):Côté AL,Byrne KT,Steinberg SM,Zhang P,Turk MJ PubMed Article URL:

5 was used in Flow cytometry/cell sorting to study how a targeted Calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment. Mouse / 1:200 Nature communications (May 2017; 8: null) "Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment." Author(s):Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M PubMed Article URL: was used in Flow cytometry/cell sorting to demonstrate that Smad2 and Smad3 are necessary for IL-9 production from T cells in an OVA-induced asthma model. Journal of immunology (Baltimore, Md. : 1950) (Sep 2013; 191: 2360) "Smad2/3 and IRF4 play a cooperative role in IL-9-producing T cell induction." Author(s):Tamiya T,Ichiyama K,Kotani H,Fukaya T,Sekiya T,Shichita T,Honma K,Yui K,Matsuyama T,Nakao T,Fukuyama S, Inoue H,Nomura M,Yoshimura A PubMed Article URL: was used in Flow cytometry/cell sorting to assess a novel approach for betaherpesvirus vaccination. Journal of virology (Aug 2010; 84: 7730) "A spread-deficient cytomegalovirus for assessment of first-target cells in vaccination." Author(s):Mohr CA,Arapovic J,Mühlbach H,Panzer M,Weyn A,Dölken L,Krmpotic A,Voehringer D,Ruzsics Z,Koszinowski U, Sacher T PubMed Article URL: