Supplemental Figure S1
|
|
|
- Virgil Bates
- 5 years ago
- Views:
Transcription
1 Supplemental Figure S1 Children from Nandi (low pathogen burden area) and Kisumu (high pathogen burden area) have significantly different pathogen-specific serological profiles. Serum antibody titers for Plasmodium falciparum (Pf) malaria, EBV, schistosomiasis, were measured at a 4 year interval (toddlers to school-age) by suspension bead-based multiplex assay. Antibodies to vaccine antigens, i.e. measles and Tetanus toxoid were measured as controls. Nandi (low pathogen), n = 33; Kisumu (high pathogen), n = 31. Antibody titers (IgG) specific for Pf (HRP1, MSP1-FVO, CSP), Measles virus (Edmonston vaccine strain), Clostridium tetani (Tetanus), Schistosoma mansoni worm antigen protein (SWAP) and EBV
2 (EAD, ZEBRA, VCA, EBNA1) were measured using multiplex conjugated-bead suspension assay. Data on the boxplot (median and 95% IQR) displays the relative amount of antibody titers in toddlers [a] and school-age children [b] from Nandi (white bar) and Kisumu (grey bar). While open circles represent outliers, computed two-tailed unpaired t-test with Welch s correction p-values are displayed on each graph (* P < 0.05, *** P < 0.001, **** P < ).
3 Supplemental Figure S2 CD3 + CD8 dim T cells are predominantly TCRab and not TCRgd T cells Peripheral blood mononuclear cells (PBMCs) were stained with fluorescently labeled antibodies and analyzed with a multiparameter flow cytometer. (A) Representative bivariate plot displaying the gating strategy for CD3 + CD8 dim T cells from school-age children living in low pathogen (n = 4) and high pathogen (n = 4) burden areas. (B) Boxplot (median + 95%
4 IQR) displaying cumulative proportions of CD8 dim T cells expressing TCRab and TCRgd. (C) Boxplot (median + 95% IQR) showing cumulative proportions of CD8 dim T cells expressing surface CD56. Data generated from one experiment. Black dots represent individual patients. (D) Representative bivariate of CD3 + CD8 dim T cells from North-American (n = 4) and African adults (n = 4) PBMCs. (E) Boxplot (median + 95% IQR) displaying cumulative proportions of CD8 dim T cells expressing TCRab and TCRgd. (F) Boxplot (median + 95% IQR) displaying cumulative proportions of CD8 dim T cells expressing surface CD56. Black dots represent individual patients. Outliers are depicted as red dots on the graph. Data generated from one experiment.
5 Supplemental Figure S3 Flow cytometry validation of differentially expressed targets identified via RNA-Seq between CD8 bright and CD8 dim TEM cells Peripheral blood mononuclear cells (PBMCs) were from Nandi (n = 7) and Kisumu (n = 7) school-age children were stained with fluorescent antibodies and analyzed with a multiparameter flow cytometer. (A) Representative histograms of antibody staining on unstimulated T EM CD8 + T cells. (D) Boxplot (median and 95% IQR) of MFI from (A). Welch s two-tailed t test p-values are displayed (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < ).
6 Supplemental Figure S4 Children from Nandi and Kisumu have comparable proportions of CD8+ T cell subsets expressing Tim-3 Peripheral blood mononuclear cells (PBMCs) were stained with fluorescently labeled antibodies. Boxplot (median and 95% IQR) displaying the proportion of CD8 dim and CD8 bright T cell subsets expressing Tim-3 from toddlers and school-age children from Nandi (low pathogen burden, N = 14) and Kisumu (high pathogen burden, N = 15). There was no
7 statistical significance between CD8+ T cell subsets expressing Tim-3 based on pathogen burden after Welch s t-test correction however there was a trend for Tim-3 expression to be lost as children aged. Data cumulated from 9 independent experiments.
Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12
1 Supplementary Data Figure legends Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 serum levels measured by multiplex ELISA (Luminex) in FL patients before
HD1 (FLU) HD2 (EBV) HD2 (FLU)
ramer staining + anti-pe beads ramer staining a HD1 (FLU) HD2 (EBV) HD2 (FLU).73.11.56.46.24 1.12 b CD127 + c CD127 + d CD127 - e CD127 - PD1 - PD1 + PD1 + PD1-1 1 1 1 %CD127 + PD1-8 6 4 2 + anti-pe %CD127
Supplementary Figure 1. BMS enhances human T cell activation in vitro in a
Supplementary Figure 1. BMS98662 enhances human T cell activation in vitro in a concentration-dependent manner. Jurkat T cells were activated with anti-cd3 and anti-cd28 antibody in the presence of titrated
x Lymphocyte count /µl CD8+ count/µl 800 Calculated
% Lymphocyte in CBC A. 50 40 30 20 10 Lymphocyte count /µl B. x10 3 2.5 1.5 C. 50 D. 1000 % CD3+CD8+ Cells 40 30 20 Calculated CD8+ count/µl 800 600 400 200 10 0 #61 #63 #64 #65 #68 #71 #72 #75 Figure
<20 <20 <20 < <20 <20 <20 <20. Mock
Cross-Lineage Neutralization PRNT 80 Titers Asian Asian West African Indian Ocean Group NHP Strain 181/25 Strain 99659 Strain 37997 Strain LR 142590 80 80 20 40 EILV/CHIKV 150844 640 640 160 320 Mock 150849
Supplementary Figure 1
Supplementary Figure 1 Identification of IFN-γ-producing CD8 + and CD4 + T cells with naive phenotype by alternative gating and sample-processing strategies. a. Contour 5% probability plots show definition
IgG3 regulates tissue-like memory B cells in HIV-infected individuals
SUPPLEMENTARY INFORMATION Articles https://doi.org/10.1038/s41590-018-0180-5 In the format provided by the authors and unedited. IgG3 regulates tissue-like memory B cells in HIV-infected individuals Lela
Kelly A. Curtis, M. Susan Kennedy, Kevin Delaney, Man Charurat, and S. Michele Owen
Kelly A. Curtis, M. Susan Kennedy, Kevin Delaney, Man Charurat, and S. Michele Owen Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA Advantages: Obvious evolution
Therapeutic PD L1 and LAG 3 blockade rapidly clears established blood stage Plasmodium infection
Supplementary Information Therapeutic PD L1 and LAG 3 blockade rapidly clears established blood stage Plasmodium infection Noah S. Butler, Jacqueline Moebius, Lecia L. Pewe, Boubacar Traore, Ogobara K.
5 Cell recognition and the immune system Exam-style questions. AQA Biology. 1 Figure 1 shows a phagocyte attracted to a bacterium.
1 Figure 1 shows a phagocyte attracted to a bacterium. Figure 1 Describe how the phagocyte destroys the bacterium. (5 marks) 2 A child was given two vaccinations consisting of antigens from the virus which
Supplementary Figure 1. Double-staining immunofluorescence analysis of invasive colon and breast cancers. Specimens from invasive ductal breast
Supplementary Figure 1. Double-staining immunofluorescence analysis of invasive colon and breast cancers. Specimens from invasive ductal breast carcinoma (a) and colon adenocarcinoma (b) were staining
Nature Protocols: doi: /nprot Supplementary Figure 1
Supplementary Figure 1 Traditional electronic gating strategy for analysing cell death based on A5-FITC and 7-AAD. a, Flow cytometry analysis showing the traditional two-stage electronic gating strategy
ankylosing spondylitis Department of Clinical Immunology, Xijing Hospital, The Fourth Military
Functional defects in CD4 + CD25 high FoxP3 + regulatory cells in ankylosing spondylitis Huifang Guo 1, 2, 3, Ming Zheng 1, 2, 3, Kui Zhang 1, 3, Fengfan Yang 1, 3, Xin Zhang 1, 3, Qing Han 1, 3, Zhi-Nan
Understandable Statistics
Understandable Statistics correlated to the Advanced Placement Program Course Description for Statistics Prepared for Alabama CC2 6/2003 2003 Understandable Statistics 2003 correlated to the Advanced Placement
Figure S1. Gating strategy used in NK cells and γδ T lymphocytes coculture An example of flow cytometry analysis shows the gating of NK cells and γδ
Figure S1. Gating strategy used in NK cells and γδ T lymphocytes coculture An example of flow cytometry analysis shows the gating of NK cells and γδ T lymphocytes used in all NK activation and cytotoxicity
Nature Immunology doi: /ni.2771
Supplementary Figure 1. Lymphadenopathy, mitogen response, effector cells, and serum Ig assessment. (a) Computerized Tomography (CT) images demonstrating lymphadenopathy (arrows) in patient F.II.1 and
THE IMMUNE SYSTEM Chapter 40 (Miller & Levine)
THE IMMUNE SYSTEM Chapter 40 (Miller & Levine) 40 1 Infectious Disease A. How Diseases Are Spread 1. Vectors animals that carry disease-causing organisms from person to person (ticks, mosquitos, other
Supplementary Figure 1. ALVAC-protein vaccines and macaque immunization. (A) Maximum likelihood
Supplementary Figure 1. ALVAC-protein vaccines and macaque immunization. (A) Maximum likelihood tree illustrating CRF01_AE gp120 protein sequence relationships between 107 Envs sampled in the RV144 trial
Supplementary Data. Treg phenotype
Supplementary Data Additional Experiment An additional experiment was performed using cryopreserved peripheral blood mononuclear cells (PBMC) derived from five renal cell carcinoma (RCC) patients [see
Supplementary Figure 1
Supplementary Figure 1 FMO control (no HLA-DR antibody) FMO control (no HLA-DR antibody) Supplementary Figure 1. Gating strategy for HLA-DR+ T-cells. A gate was drawn around lymphocytes based on cell size
Fluorochrome Panel 1 Panel 2 Panel 3 Panel 4 Panel 5 CTLA-4 CTLA-4 CD15 CD3 FITC. Bio) PD-1 (MIH4, BD) ICOS (C398.4A, Biolegend) PD-L1 (MIH1, BD)
Additional file : Table S. Antibodies used for panel stain to identify peripheral immune cell subsets. Panel : PD- signaling; Panel : CD + T cells, CD + T cells, B cells; Panel : Tregs; Panel :, -T, cdc,
Th17 and Th17/Treg ratio at early HIV infection associate with protective HIV-specific CD8 + T-cell responses and disease progression
Th17 and Th17/Treg ratio at early HIV infection associate with protective HIV-specific CD8 T-cell responses and disease progression Juliana Falivene 1, Yanina Ghiglione 1, Natalia Laufer 1,3, María Eugenia
Supplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
Supplementary Figure 1. EBV + SMT show latency type III. (a) P2.1 immunohistochemistry of the EBV proteins LMP1 (negative), LMP2A (scattered
Supplementary Figure 1. EBV + SMT show latency type III. (a) P2.1 immunohistochemistry of the EBV proteins LMP1 (negative), LMP2A (scattered positive), EBNA2 (partially positive), ZEBRA (negative), of
Online Appendix Material and Methods: Pancreatic RNA isolation and quantitative real-time (q)rt-pcr. Mice were fasted overnight and killed 1 hour (h)
Online Appendix Material and Methods: Pancreatic RNA isolation and quantitative real-time (q)rt-pcr. Mice were fasted overnight and killed 1 hour (h) after feeding. A small slice (~5-1 mm 3 ) was taken
Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4
Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4 Ankita Garg, Rodney Trout and Stephen A. Spector,,* Department
Supplemental Information. Aryl Hydrocarbon Receptor Controls. Monocyte Differentiation. into Dendritic Cells versus Macrophages
Immunity, Volume 47 Supplemental Information Aryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages Christel Goudot, Alice Coillard, Alexandra-Chloé Villani,
LIAISON Measles IgG The fully automated solution for quantitative antibody detection
LIAISON Measles IgG The fully automated solution for quantitative antibody detection FOR OUTSIDE THE US AND CANADA ONLY LIAISON Measles IgG Number of tests 100 Key assay features Method Assay range Solid
a Beckman Coulter Life Sciences: White Paper
a Beckman Coulter Life Sciences: White Paper An 8-color DuraClone IM panel for detection of Human blood dendritic cells by flow cytometry Nathalie Dupas 1, Snehita Sattiraju 2, Neha Girish 2, Murthy Pendyala
Pearson r = P (one-tailed) = n = 9
8F4-Specific Lysis, % 1 UPN1 UPN3 8 UPN7 6 Pearson r =.69 UPN2 UPN5 P (one-tailed) =.192 4 UPN8 n = 9 2 UPN9 UPN4 UPN6 5 1 15 2 25 8 8F4, % Max MFI Supplementary Figure S1. AML samples UPN1-UPN9 show variable
Eosinophils are required. for the maintenance of plasma cells in the bone marrow
Eosinophils are required for the maintenance of plasma cells in the bone marrow Van Trung Chu, Anja Fröhlich, Gudrun Steinhauser, Tobias Scheel, Toralf Roch, Simon Fillatreau, James J. Lee, Max Löhning
Supplementary Fig. 1: Ex vivo tetramer enrichment with anti-c-myc beads
Supplementary Fig. 1: Ex vivo tetramer enrichment with anti-c-myc beads Representative example of comparative ex vivo tetramer enrichment performed in three independent experiments with either conventional
AbSeq on the BD Rhapsody system: Exploration of single-cell gene regulation by simultaneous digital mrna and protein quantification
BD AbSeq on the BD Rhapsody system: Exploration of single-cell gene regulation by simultaneous digital mrna and protein quantification Overview of BD AbSeq antibody-oligonucleotide conjugates. High-throughput
Disease: any change, other than an injury, that disrupts homeostasis. Pathogen: disease-causing agent such as bacteria, virus, etc.
The Immune System Disease: any change, other than an injury, that disrupts homeostasis. Pathogen: disease-causing agent such as bacteria, virus, etc. Infectious disease- caused by pathogen Germ theory
Supplementary Materials for
www.sciencemag.org/content/348/6241/aaa825/suppl/dc1 Supplementary Materials for A mucosal vaccine against Chlamydia trachomatis generates two waves of protective memory T cells Georg Stary,* Andrew Olive,
Vaccine Delivery and TLR Ligands Influence the Quality of T cell Responses in NHP. Robert A. Seder, M.D. Vaccine Research Center NIAID, NIH
Vaccine Delivery and TLR Ligands Influence the Quality of T cell Responses in NHP Robert A. Seder, M.D. Vaccine Research Center NIAID, NIH Vaccines Against HIV, Malaria and Tuberculosis Will Require Neutralizing
ASTARTE IN ACTION. Using a Recall Antigen Assay as a Tool for Understanding Immunity CASE STUDY
ASTARTE IN ACTION Using a Recall Antigen Assay as a Tool for Understanding Immunity CASE STUDY Introduction While there is no industry-accepted protocol for measuring the functionality of peripheral blood
Nature Medicine: doi: /nm.2109
HIV 1 Infects Multipotent Progenitor Cells Causing Cell Death and Establishing Latent Cellular Reservoirs Christoph C. Carter, Adewunmi Onafuwa Nuga, Lucy A. M c Namara, James Riddell IV, Dale Bixby, Michael
VUmc Basispresentatie
Clinical diagnostic cytometry Gerrit J Schuurhuis Dept of Hematology VU University Medical Center Amsterdam, Netherlands Use of immunophenotyping at diagnosis to trace residual disease after therapy 1.
SDC (Supplemental Digital Content) Figure S1. Percentages of Granzyme B expressing CD8 + T lymphocytes
Figure S1. Percentages of Granzyme expressing D8 + T lymphocytes 13.5% 1.4%.6% 77% 21% 24% 76% 8 8 Granzyme + (%) 6 4 2 Granzyme + (%) 6 4 2 D8 + Tnaive Tcm Tem Temra D8 + D8 + D28 + D8 + D28 null We analyzed
Supplemental Information. Checkpoint Blockade Immunotherapy. Induces Dynamic Changes. in PD-1 CD8 + Tumor-Infiltrating T Cells
Immunity, Volume 50 Supplemental Information Checkpoint Blockade Immunotherapy Induces Dynamic Changes in PD-1 CD8 + Tumor-Infiltrating T Cells Sema Kurtulus, Asaf Madi, Giulia Escobar, Max Klapholz, Jackson
Nature Immunology: doi: /ni Supplementary Figure 1. Transcriptional program of the TE and MP CD8 + T cell subsets.
Supplementary Figure 1 Transcriptional program of the TE and MP CD8 + T cell subsets. (a) Comparison of gene expression of TE and MP CD8 + T cell subsets by microarray. Genes that are 1.5-fold upregulated
Human and mouse T cell regulation mediated by soluble CD52 interaction with Siglec-10. Esther Bandala-Sanchez, Yuxia Zhang, Simone Reinwald,
Human and mouse T cell regulation mediated by soluble CD52 interaction with Siglec-1 Esther Bandala-Sanchez, Yuxia Zhang, Simone Reinwald, James A. Dromey, Bo Han Lee, Junyan Qian, Ralph M Böhmer and Leonard
Supplementary Figure 1. Immune profiles of untreated and PD-1 blockade resistant EGFR and Kras mouse lung tumors (a) Total lung weight of untreated
1 Supplementary Figure 1. Immune profiles of untreated and PD-1 blockade resistant EGFR and Kras mouse lung tumors (a) Total lung weight of untreated (U) EGFR TL mice (n=7), Kras mice (n=7), PD-1 blockade
Malaria parasite vaccine development Strategies & Targets
Malaria parasite vaccine development Strategies & Targets Tulane University Ahmed Aly Most malaria disease deaths are among children and pregnant women A child or a pregnant woman dies of malaria nearly
MHC MULTIMER PROFICIENCY PANEL 2017
MHC MULTIMER PROFICIENCY PANEL 2017 August 2017 CONTACT Charlotte Halgreen ProficiencyPanel@immudex.com FOR MORE INFORMATION www.proficiencypanel.com MHC MULTIMER PROFICIENCY PANEL 2017 This report summarizes
ELISA Range. VIROTECH Diagnostics GmbH. Lot independent. Reagent. System. IgG-Conjugate. Substrate. IgM-Conjugate. Washing Solution.
Quelle: www.fotolia.com ELISA Range IgG-Conjugate IgM-Conjugate IgA-Conjugate Lot independent Reagent System Stop Solution Substrate Washing Solution Serum Dilution Dilution Buffer Incubation Time Cerebrospinal
SUPPLEMENT Supplementary Figure 1: (A) (B)
SUPPLEMENT Supplementary Figure 1: CD4 + naïve effector T cells (CD4 effector) were labeled with CFSE, stimulated with α-cd2/cd3/cd28 coated beads (at 2 beads/cell) and cultured alone or cocultured with
and follicular helper T cells is Egr2-dependent. (a) Diagrammatic representation of the
Supplementary Figure 1. LAG3 + Treg-mediated regulation of germinal center B cells and follicular helper T cells is Egr2-dependent. (a) Diagrammatic representation of the experimental protocol for the
CHAPTER 3 Describing Relationships
CHAPTER 3 Describing Relationships 3.1 Scatterplots and Correlation The Practice of Statistics, 5th Edition Starnes, Tabor, Yates, Moore Bedford Freeman Worth Publishers Reading Quiz 3.1 True/False 1.
MATERIALS AND METHODS. Neutralizing antibodies specific to mouse Dll1, Dll4, J1 and J2 were prepared as described. 1,2 All
MATERIALS AND METHODS Antibodies (Abs), flow cytometry analysis and cell lines Neutralizing antibodies specific to mouse Dll1, Dll4, J1 and J2 were prepared as described. 1,2 All other antibodies used
Identification and Characterization of CD4 T cells actively transcribing HIV RNA in Peripheral Blood
Dale and Betty Bumpers Vaccine Research Center National Institute of Allergy and Infectious Diseases National Institutes of Health Identification and Characterization of CD4 T cells actively transcribing
In vitro human regulatory T cell expansion
- 1 - Human CD4 + CD25 + CD127 dim/- regulatory T cell Workflow isolation, in vitro expansion and analysis In vitro human regulatory T cell expansion Introduction Regulatory T (Treg) cells are a subpopulation
Dual Targeting Nanoparticle Stimulates the Immune
Dual Targeting Nanoparticle Stimulates the Immune System to Inhibit Tumor Growth Alyssa K. Kosmides, John-William Sidhom, Andrew Fraser, Catherine A. Bessell, Jonathan P. Schneck * Supplemental Figure
In vitro human regulatory T cell suppression assay
Human CD4 + CD25 + regulatory T cell isolation, in vitro suppression assay and analysis In vitro human regulatory T cell suppression assay Introduction Regulatory T (Treg) cells are a subpopulation of
Understanding Poor Vaccine Responses: Transcriptomics of Vaccine Failure
Final Report - Summer Research Project 1 Introduction Understanding Poor Vaccine Responses: Transcriptomics of Vaccine Failure Katherine Miller, Year 3, Dalhousie University (Supervisor: Dr. Lisa Barrett,
M I C R O B I O L O G Y
ninth edition TORTORA FUNKE CASE M I C R O B I O L O G Y a n i n t r o d u c t i o n 18 Practical Applications of Immunology PowerPoint Lecture Slide Presentation prepared by Christine L. Case Vaccine
PD-1 and its ligand PD-L1 are progressively up-regulated on CD4 and CD8 T-cells. in HIV-2 infection irrespective of the presence of viremia
Tendeiro et al_ Supplemental Digital ontent_page of PD and its ligand PDL are progressively upregulated on D4 and D8 Tcells in infection irrespective of the presence of viremia Supplemental Digital ontent
Supplemental materials
Supplemental materials 1 Supplemental Fig. 1 Immunogram This immunogram summarizes patient clinical data and immune parameters at corresponding time points for Patient IMF-32. The top panel illustrates
CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads (Miltenyi Biotec) for depletion of CD25 +
Supplements Supplemental Materials and Methods Depletion of CD25 + T-cells from PBMC. Fresh or HD precultured PBMC were stained with the conjugate CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads
ILC1 and ILC3 isolation and culture Following cell sorting, we confirmed that the recovered cells belonged to the ILC1, ILC2 and
Supplementary Methods and isolation and culture Following cell sorting, we confirmed that the recovered cells belonged to the, ILC2 and subsets. For this purpose we performed intracellular flow cytometry
Nature Immunology: doi: /ni Supplementary Figure 1. Gene expression profile of CD4 + T cells and CTL responses in Bcl6-deficient mice.
Supplementary Figure 1 Gene expression profile of CD4 + T cells and CTL responses in Bcl6-deficient mice. (a) Gene expression profile in the resting CD4 + T cells were analyzed by an Affymetrix microarray
activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows
Supplemental Data Supplemental Figure 1 compares CXCR4 expression in untreated CD8 + T cells, following activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows the
Supplementary Figure 1. Enhanced detection of CTLA-4 on the surface of HIV-specific
SUPPLEMENTARY FIGURE LEGEND Supplementary Figure 1. Enhanced detection of CTLA-4 on the surface of HIV-specific CD4 + T cells correlates with intracellular CTLA-4 levels. (a) Comparative CTLA-4 levels
pplementary Figur Supplementary Figure 1. a.
pplementary Figur Supplementary Figure 1. a. Quantification by RT-qPCR of YFV-17D and YFV-17D pol- (+) RNA in the supernatant of cultured Huh7.5 cells following viral RNA electroporation of respective
Supplementary Figure 1. Using DNA barcode-labeled MHC multimers to generate TCR fingerprints
Supplementary Figure 1 Using DNA barcode-labeled MHC multimers to generate TCR fingerprints (a) Schematic overview of the workflow behind a TCR fingerprint. Each peptide position of the original peptide
Supplementary Figure 1 Chemokine and chemokine receptor expression during muscle regeneration (a) Analysis of CR3CR1 mrna expression by real time-pcr
Supplementary Figure 1 Chemokine and chemokine receptor expression during muscle regeneration (a) Analysis of CR3CR1 mrna expression by real time-pcr at day 0, 1, 4, 10 and 21 post- muscle injury. (b)
Malaria and Helminth. Ayola Akim ADEGNIKA CERMEL; Lambaréné, Gabon
Malaria and Helminth Ayola Akim ADEGNIKA CERMEL; Lambaréné, Gabon aadegnika@cermel.org OVERVIEW Objectives Background Hypothesis Litterature review Malaria and Helminthiasis in Gabon Epidemiology vaccinology
supplemental Figure 1
supplemental Figure 1 A T cell T1 anti-ny-eso-117-16/hla-a*:1 CDζ CH/CH scfv B T cell T1 anti-ny-eso-117-16/hla-a*:1 CDζ CH/CH scfv C T cell BW1/6 anti-cea CDζ CH/CH scfv supplemental Figure 1.79.9.87
Supplementary Figure 1.
Supplementary Figure 1. Female Pro-ins2 -/- mice at 5-6 weeks of age were either inoculated i.p. with a single dose of CVB4 (1x10 5 PFU/mouse) or PBS and treated with αgalcer or control vehicle. On day
Increased IL-12 induced STAT-4 signaling in CD8 T cells. from aged mice
Increased IL-2 induced STAT-4 signaling in CD8 T cells from aged mice Erin Rottinghaus * Abstract: Aging is associated with poor immune function leading to increased susceptibility to infectious diseases
Serological markers to measure recent changes in malaria at population level in Cambodia
DOI 10.1186/s12936-016-1576-z Malaria Journal RESEARCH Open Access Serological markers to measure recent changes in malaria at population level in Cambodia Karen Kerkhof 1,2*, Vincent Sluydts 1,3, Laura
Progressive Telomere Shortening of Epstein-Barr Virus Specific Memory T Cells during HIV Infection: Contributor to Exhaustion?
BRIEF REPORT Progressive Telomere Shortening of Epstein-Barr Virus Specific Memory T Cells during HIV Infection: Contributor to Exhaustion? Debbie van Baarle, 1 Nening M. Nanlohy, 1 Sigrid Otto, 1 Fiona
The Dubbo Infection Outcomes Study
The Dubbo Infection Outcomes Study Determinants of protracted illness after acute infection Dubbo Infection Outcomes Study Three parallel cohorts Epstein-Barr virus: - infectious mononucleosis - established
MHC MULTIMER PROFICIENCY PANEL 2015
MHC MULTIMER PROFICIENCY PANEL 2015 July 2015 CONTACT Charlotte Halgreen ProficiencyPanel@immudex.com FOR MORE INFORMATION www.proficiencypanel.com MHC MULTIMER PROFICIENCY PANEL 2015 This report summarizes
Intermediate monocytes in ANCA vasculitis: increased surface. expression of ANCA autoantigens and IL-1β secretion in response. to anti-mpo antibodies
Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-mpo antibodies Eóin C. O Brien 1, Wayel H. Abdulahad 2, Abraham Rutgers
SUPPLEMENTAL MATERIAL. Number of patients 14
SUPPLEMENTAL MATERIAL Supplemental Table 1 Number of patients 14 Age, years 54.9 ± 10.0 Female gender, n (%) 6 (42.9) Diabetes, n (%) 2 (14.3) History of hypertension, n (%) 5 (35.7) Ever smoker, n (%)
Supplemental Table 1. Primer sequences for transcript analysis
Supplemental Table 1. Primer sequences for transcript analysis Primer Sequence (5 3 ) Primer Sequence (5 3 ) Mmp2 Forward CCCGTGTGGCCCTC Mmp15 Forward CGGGGCTGGCT Reverse GCTCTCCCGGTTTC Reverse CCTGGTGTGCCTGCTC
Defective STAT1 activation associated with impaired IFN-g production in NK and T lymphocytes from metastatic melanoma patients treated with IL-2
Defective STAT1 activation associated with impaired IFN-g production in NK and T lymphocytes from metastatic melanoma patients treated with IL-2 SUPPLEMENTARY FIGURES AND TABLES Supplementary Figure S1:
Technical Resources. BD Immunocytometry Systems. FastImmune Intracellular Cytokine Staining Procedures
FastImmune Intracellular Cytokine Staining Procedures BD has developed protocols for the detection of intracellular cytokines in activated lymphocytes and in activated monocytes. The procedures have been
MINUTE TO WIN IT: NAMING THE PRESIDENTS OF THE UNITED STATES
MINUTE TO WIN IT: NAMING THE PRESIDENTS OF THE UNITED STATES THE PRESIDENTS OF THE UNITED STATES Project: Focus on the Presidents of the United States Objective: See how many Presidents of the United States
Vaccinology 101 for Fellows
Vaccinology 101 for Fellows Meg Fisher, MD Medical Director, The Children s Hospital Monmouth Medical Center An affiliate of the Saint Barnabas Health Care System Long Branch, NJ Disclosures I have no
Suppl Video: Tumor cells (green) and monocytes (white) are seeded on a confluent endothelial
Supplementary Information Häuselmann et al. Monocyte induction of E-selectin-mediated endothelial activation releases VE-cadherin junctions to promote tumor cell extravasation in the metastasis cascade
Detailed step-by-step operating procedures for NK cell and CTL degranulation assays
Supplemental methods Detailed step-by-step operating procedures for NK cell and CTL degranulation assays Materials PBMC isolated from patients, relatives and healthy donors as control K562 cells (ATCC,
Pathogens, Antibodies, and Vaccines
STO-138 Pathogens, Antibodies, and Vaccines Part 1: Modeling Pathogens and Antibodies Three dangerous diseases: Pertussis (whooping cough) is caused by Bordetella pertussis bacteria Diphtheria is caused
Identify two variables. Classify them as explanatory or response and quantitative or explanatory.
OLI Module 2 - Examining Relationships Objective Summarize and describe the distribution of a categorical variable in context. Generate and interpret several different graphical displays of the distribution
A step-by-step approach to build and analyze a multicolor panel
Analyze A step-by-step approach to build and analyze a multicolor panel For Research Use Only. Not for use in diagnostic or therapeutic procedures. Alexa Fluor is a registered trademark of Life Technologies
Seroprevalence of Tetanus Antibody in the Thai Population: A National Survey
ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY (2007) 25: 219-223 Seroprevalence of Tetanus Antibody in the Thai Population: A National Survey Pantipa Chatchatee 1, Susheera Chatproedprai 1, Porpit Warinsathien
Rapid Flow Cytometry Method for Quantitation of LFA-1-Adhesive T Cells
CLINICAL AND VACCINE IMMUNOLOGY, Mar. 2006, p. 403 408 Vol. 13, No. 3 1556-6811/06/$08.00 0 doi:10.1128/cvi.13.3.403 408.2006 Rapid Flow Cytometry Method for Quantitation of LFA-1-Adhesive T Cells Brian
EBV Infection and Immunity. Andrew Hislop Institute for Cancer Studies University of Birmingham
EBV Infection and Immunity Andrew Hislop Institute for Cancer Studies University of Birmingham EBV Introduction Large ds DNA virus Spread by saliva contact Lifelong infection Predominantly B-lymphotropic
Supplementary Figure 1. Ex vivo IFNγ production by Tregs. Nature Medicine doi: /nm % CD127. Empty SSC 98.79% CD25 CD45RA.
SSC CD25 1.8% CD127 Empty 98.79% FSC CD45RA CD45RA Foxp3 %IFNγ + cells 4 3 2 1 + IL-12 P =.3 IFNγ p=.9 %IL-4+ cells 3 2 1 IL-4 P =.4 c %IL-1 + cells IFNγ 4 3 2 1 Control Foxp3 IL-1 P =.41.64 4.76 MS 2.96
Supplemental Information. Systems Scale Interactive Exploration. Reveals Quantitative and Qualitative Differences
Immunity, Volume 38 Supplemental Information Systems Scale Interactive Exploration Reveals Quantitative and Qualitative Differences in Response to Influenza and Pneumococcal Vaccines Gerlinde Obermoser,
ImageStream cytometer analysis. Cells were cultured as described above in vented-cap
ImageStream cytometer analysis. Cells were cultured as described above in vented-cap polypropylene tubes, stained with αcd66b-fitc, αm-dc8-pe and αcd56-pe-cy5.5 mabs, washed and fixed with 4 % (w/v) paraformaldehyde.
TITLE: Fusions of Breast Carcinoma and Dendritic Cells as a Vaccine for the Treatment of Metatastic Breast Cancer
AD Award Number: DAMD17-03-1-0487 TITLE: Fusions of Breast Carcinoma and Dendritic Cells as a Vaccine for the Treatment of Metatastic Breast Cancer PRINCIPAL INVESTIGATOR: Donald Kufe, M.D. CONTRACTING
Difference in Cytokine Production and Cell Activation between Adenoidal Lymphocytes and Peripheral Blood Lymphocytes of Children with Otitis Media
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Sept. 2005, p. 1130 1134 Vol. 12, No. 9 1071-412X/05/$08.00 0 doi:10.1128/cdli.12.9.1130 1134.2005 Copyright 2005, American Society for Microbiology. All
PREPARATION STANDARD MATERIAL HELD AT CODE WHO/BS DOCUMENT. 1st Reference Reagent, 2009
WHO International Biological Reference Preparations Held and Distributed by the WHO International Laboratories for Biological Standards Acellular pertussis vaccine for potency assay by modified mouse challenge
Supporting Information Table of Contents
Supporting Information Table of Contents Supporting Information Figure 1 Page 2 Supporting Information Figure 2 Page 4 Supporting Information Figure 3 Page 5 Supporting Information Figure 4 Page 6 Supporting
Ministry of Health, Screening and Vaccination Requirements from 1 January 2019
Ministry of Health, Screening and Vaccination Requirements from 1 January 2019 Mumps, Measles and Rubella (MMR) All students should be immune or vaccinated. Documented proof of vaccination (2-dose series);
Supplementary Table; Supplementary Figures and legends S1-S21; Supplementary Materials and Methods
Silva et al. PTEN posttranslational inactivation and hyperactivation of the PI3K/Akt pathway sustain primary T cell leukemia viability Supplementary Table; Supplementary Figures and legends S1-S21; Supplementary
Muse Assays for Cell Analysis
Muse Assays for Cell Analysis Multiple Assay Outputs for Cell Analysis Cell Health Cell Signalling Immunology Muse Count & Viability Kit Muse Cell Cycle Kit Muse Annexin V & Dead Cell Kit Muse Caspase