CURRICULUM VITAE DR. SHYAM BABU PRASAD
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1 CURRICULUM VITAE DR. SHYAM BABU PRASAD (Assistant Professor) Department of Microbiology, Central University of Rajasthan, Dist-Ajmer, (Rajasthan) Mobile No EDUCATIONAL QUALIFICATIONS: Ph.D. (2013), Awarded in Molecular Biology and Biotechnology, thesis entitled PI3K/AKT signaling pathway: Molecular Genetic and Epigenetic studies in Cervical Cancer from Department of Molecular & Human Genetics, Banaras Hindu University, Varanasi, India. Master of Science (M.Sc.) in Molecular Biology and Biotechnology ( ) Department of Molecular Biology and Biotechnolgy, Tezpur University, Assam, India: CGPA 7.54/10. Bachelor of Science (B.Sc.) in Chemistry Honors ( ): First Class from J. P. University, Chapra, India. RESEARCH PUBLICATIONS: 1. Prasad SB and Bharti AC: Relevance STAT3 signaling and Epithelial Mesenchymal Transition (EMT) phenotype in progression of Cervical Cancer Metastasis (Under preparation). 2. Prasad SB, Das M, Yadav SS, Kumari S, Pandey LK, Singh S, Pradhan S, Narayan G Prevalence of high risk HPV types in cervical cancer patients of eastern Uttar Pradesh, India". International Journal of Gynecological Pathology (Under review). 3. Kumari S, Prasad SB, Yadav SS, Kumar M, Nath G, Khana A, Dixit VK, Puneet, Singh S, Narayan G (2016). Cyclin D1 and Cyclin E2 are Differentially Expressed in Gastric Cancer. Medical Oncology 33(5):40.doi: /s (IF 2.63). [PMID: ] 4. Yadav SS, Prasad SB, Prasad CB, Pandey LK, Pradhan S, Singh S,, Narayan G (2016). CXCL12 is a key regulator in tumor microenvironment of cervical cancer: an in vitro study. Clinical & Experimental Metastasis; DOI /s (IF 3.49). [PMID: ] 1
2 5. Das M, Prasad SB, Yadav SS, Modi A, Singh S, Pradhan S, Narayan G (2015): HPV type specific response of cervical cancer cells to cisplatin after silencing replication licensing factor MCM4. Tumor Biology; DOI: /s (IF: 3.61). [ PMID: ] 6. Prasad SB, Das M, Yadav SS, Kumari S, Pandey LK, Singh S, Pradhan S, Narayan G (2015): PI3K/AKT pathway mediated regulation of p27kip1 is associated with cell cycle arrest and apoptosis in cervical cancer. Cellular Oncology; 38(3):215-25, doi: /s x (IF: 3.03). [ PMID: ] 7. Yadav SS, Prasad SB, Das M, Kumari S, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G (2015): Staurosporine inhibits the stoma cell induced expression of MMPs and tumor promoting events in cervical cancer cell lines. Life Science; 133:21-8, doi: /j.lfs , (IF: 2.70). [ PMID: ] 8. Chaturmohta A, Dixit R, Narayan G, Gupta P, Prasad SB, Yadav SS and Shukla VK (2015): Do Expression Profiles of Cytokines VEGF, TNF- α, IL-1β, IL-6 and IL-8 Correlate with Gallbladder Cancer?. Journal of Cancer Science and Clinical Oncology (Volume 1 Issue 3), ISSN: Shantanu L, Arun K, Prasad SB, Bharti AC, Veena K (2015): Folic Acid and Trastuzumab Functionalized Redox Responsive Polymersomes for Intracellular Doxorubicin Delivery in Breast Cancer. Biomacromolecules; 16(6): doi: /acs.biomac.5b0024, (IF: 5.75). [ PMID: ] 10. Prasad SB, Das M, Yadav SS, Govardhan HB, Pandey LK, Singh S, Pradhan S, Narayan G (2014): Down Regulation of FOXO1 Promotes Cell Proliferation in Cervical Cancer. Journal of Cancer; 5(8): doi: /jca.6554, (IF: 3.27). [ PMID: ] 11. Yadav SS, Prasad SB, Das M, Kumari S, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G (2014): Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer. BioMed Research International; doi: /2014/581403, (IF: 2.88). [ PMID: ] 12. Kumari S, Mishra SP, Rahul, Prasad SB, Yadav SS, Das M, Kumar M, Nath G, Khana A, Dixit VK,, Puneet, Singh S, Narayan G (2014): Differential expression of COX-2: A potential marker for clinical phenotypes of Gastric Cancer. International Journal of Advanced Research (IF: 4.58). 13. Das M, Prasad SB, Yadav SS, Govardhan HB, Pandey LK, Singh S,Pradhan S, Narayan G (2013): Over Expression of Minichromosome Maintenance Genes is Clinically Correlated to Cervical Carcinogenesis. PLoS One; 8(7):e doi: /journal.pone ; (IF: 3.73). [ PMID: ] 2
3 14. Singh M, Sharma NK, Prasad SB, Yadav SS, Narayan G, Rai AK (2013): Freshwater cyanobacterium Anabaena doliolum transformed with ApGSMT-DMT exhibited enhanced salt tolerance and protection to nitrogenase activity, but changed its behavior to halophily. Microbiology, 159(Pt 3): doi: /mic , (IF: 2.85). [PMID: ] 15. Prasad SB, Narayan G (2012): A new insight of GSK3B regulation: implications in cancer therapy. Journal of Scientific Research, Banaras Hindu University; 56: Singh S, Prasad SB, Yadav SS, Agrawal NK, Narayan G (2012): Association of common variants of CDKN2A/2B rs (C/T) and WFS1 rs (C/G) with type 2 diabetes mellitus in Indian population of eastern Uttar Pradesh. Journal of Diabetes and Metabolism; 3:9. (IF: 1.77). 17. Ramteke A, Prasad SB, Kumar S, Borah S, Das RK (2007). Leaf Extract of Phlogacanthus Tubiflorus Nees. Modulates Glutathione level in CCl 4 treated Chicken Lymphocytes. J. Assam Sc. Soc.; 47, Book Chapter: 1. Ramteke A, Prasad SB, Kumar S, Borah S, Das RK (2007). Anti-oxidative effects of Physalis minima Linn leaf extracts. Advances in Life Sciences, Studium Press, Texas, USA, TECHNICAL EXPERTISE: Cell culture Techniques Cell culture: Cell lines maintenance, handling various human cancer and normal cell lines and primary culture set up from tumor biopsies samples. MTT assay and Cell proliferation: Treatment of cell with the various drugs and dose dependent inhibition of cell viability, and cell proliferation was performed. Flow cytometry: Flow cytometric analysis for immunophenotyping by staining membrane associated and intra cellular proteins. Apoptosis assay: Acquisition and analysis of apoptotic cells by Flow cytometer against drug treatment using AnnexinV/PI staining procedure. Cell cycle analysis: Staining of drug treated cells with PI. Cells were analyzed with a fluorescence activated cell sorter analysis (FACS), and data was analyzed by FACS Caliber software (BD, USA). Microscopy: Preparation of slides for Immunofluorescence and Fluorescence microscopy. 3
4 Gene silencing: Transfection and sirna mediated gene silencing. DNA/RNA/PCR Techniques: DNA related techniques: Genomic DNA and plasmid DNA isolation, Agarose gel electrophoresis and RNA isolation, Reverse Transcriptase-PCR and Real Time PCR. HPV screening and Genotyping using PCR from Biopsy samples of cervical cancer Primer Deigning: To check expression profiles of the genes, polymorphism, and Methyl specific PCR primer using online available software tools. MSP (Methyl specific-pcr): To study of Promoter Hypermethylation of Tumor suppressor gene, Bisulfite Conversion of the genomic DNA was performed. RFLP: Restriction digestion of DNA and Agarose gel electrophoresis to check polymorphism. Comet assay (single-cell gel electrophoresis): To check the extent of DNA damage. Protein Techniques: Western Blotting: Native and SDS-PAGE, Western blot analysis (full length and Phospho specific immunodetection of the protein), Immunoprecipitation of the protein. Immunohistochemistry: Preparation of tissue block and slides of tissue section to check the expression and localization of the protein. Spectroscopic Techniques: Quantification of Protein by Spectrophotometer and expression of protein by ELISA Microplate Reader. INTERNATIONAL/NATIONAL CONFERENCES/SYMPOSIA ATTENDED: Abstract published: 1. Prasad SB and Bharti AC: Blocking STAT3 signaling abrogates epithelial mesenchymal transition in Cervical Cancer cells. 34 th Annual Convention of Indian Association for Cancer Research Cancer Research: From Bench to Bedside held at Jaipur, from February Kumari S, Prasad SB, Yadav SS, Puneet, Singh S, Narayan G: Clinical correlation of Cyclin D1 and Cyclin E2 expression in Gastric Carcinoma.. 34 th Annual Convention of 4
5 Indian Association for Cancer Research Cancer Research: From Bench to Bedside held at Jaipur, from February Arusha Modi, Prasad SB, Saeidian AH, Yadav SS, Das M, Pandey LK, Singh S, Pradhan S, Narayan G: GSK3β mediated regulation of cell proliferation in Cervical Cancer. 33 rd Annual Convention of Indian Association for Cancer Research: Discovery, Innovation and Translation in Cancer research. Jointly Organized by Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram and Srinivasa Ramanujan Institute for Basic Sciences, Kottayam February, Prasad C, Prasad SB, Yadav SS, Das M, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G: PARP inhibition revert Cisplatin resistance in cervical cancer. 33 rd Annual Convention of Indian Association for Cancer Research: Discovery, Innovation and Translation in Cancer research. Jointly Organized by Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram and Srinivasa Ramanujan Institute for Basic Sciences, Kottayam February, Narayan G, Yadav SS, Prasad SB, Das M, Kumari S, Swain PK, Pandey LK, Singh S, Pradhan S. Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer. 33 rd Annual Convention of Indian Association for Cancer Research: Discovery, Innovation and Translation in Cancer research. Jointly Organized by Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram and Srinivasa Ramanujan Institute for Basic Sciences, Kottayam February, Sunita S, Yadav SS, Prasad SB, Das M, Pandey LK, Pradhan S, Narayan G. Antitumor activity of staurosporine in cervical cancer cell lines. 33 rd Annual Convention of Indian Association for Cancer Research: Discovery, Innovation and Translation in Cancer research. Jointly Organized by Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram and Srinivasa Ramanujan Institute for Basic Sciences, Kottayam February, Prasad SB, Das M, Yadav SS, Pandey LK, Singh S, Pradhan S, Narayan G: Inhibition of PI3K/AKT pathway induces apoptosis and cell cycle arrest in the cervical cancer. 32 nd Annual Convention of Indian Association for Cancer Research Emerging Trends in Cancer Research: Road to Prevention and Cure and an International Symposium on Infection and Cancer held at ACBR, University of Delhi, North Campus from February, Kumari S, Prasad SB, Yadav SS, Das M, Mishra SP, Kumar M, Puneet, Singh S, Narayan G: Expression of COX-2 is clinically correlated to the progression of gastric carcinoma. 32 nd Annual Convention of Indian Association for Cancer Research Emerging Trends in Cancer Research: Road to Prevention and Cure and an International Symposium on Infection and Cancer held at ACBR, University of Delhi, North Campus from February,
6 9. Das M, Prasad SB, Yadav SS, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G: Enhanced expression of MCM4 predicts altered replication licensing in cervical cancer cells. 32 nd Annual Convention of Indian Association for Cancer Research Emerging Trends in Cancer Research: Road to Prevention and Cure and an International Symposium on Infection and Cancer held at ACBR, University of Delhi, North Campus from February, Prasad SB, Yadav SS, Das M, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G: PI3K/AKT signaling mediated regulation of p27 in cervical cancer progression. International Symposium on Developmental and Complex Disorders & XXXVIII Annual Conference of the Indian Society of Human Genetics, Genomics and Community Health held at BHU,Varanasi from Nov, Yadav SS, Prasad SB, Das M, Pandey LK, Singh S, Pradhan S, Narayan G: Epigenetic Regulation of SDF-1α and its Receptor CXCR4 in Cervical Carcinogenesis. International Symposium on Developmental and Complex Disorders & XXXVIII Annual Conference of the Indian Society of Human Genetics, Genomics and Community Health held at BHU,Varanasi from Nov, Das M, Prasad SB, Yadav SS, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G: Over expression of Minichromosome Maintenance genes is clinically correlated to Cervical Carcinogenesis. International Symposium on Developmental and Complex Disorders & XXXVIII Annual Conference of the Indian Society of Human Genetics, Genomics and Community Health held at BHU,Varanasi from Nov, Kumari S, Prasad SB, Yadav SS, Das M, Rahul, Kumar M, Puneet, Singh S, Narayan G: Over expression of p53 and p21 is correlated to progression of Gastric Carcinoma. International Symposium on Developmental and Complex Disorders & XXXVIII Annual Conference of the Indian Society of Human Genetics, Genomics and Community Health held at BHU,Varanasi from Nov, Prasad C, Prasad SB, Yadav SS, Das M, Swain PK, Pandey LK, Singh S, Pradhan S, Narayan G: DNA Damage Induced Expression of Cell Cycle Check Point Kinases and PARP contributes to cell survival in cervical cancer. International Symposium on Developmental and Complex Disorders & XXXVIII Annual Conference of the Indian Society of Human Genetics, Genomics and Community Health held at BHU,Varanasi from Nov, Prasad SB, Yadav SS, Das M, Goverdhan HB, Pandey LK, Singh S, Pradhan S, Narayan G: Inhibition of PI3K/AKT pathway activates FOXO1 transcription factor leading to growth arrest and apoptosis in cervical cancer. International conference on Genes, Genetics & Genomics: Today & Tomorrow- Human Concerns & XXXVII Annual conference of the Indian Society of Human Genetics, Panjab University, Chandigarh, India,. Poster Abstract No. PP 171,
7 16. Singh S, Prasad SB, Yadav SS, Agrawal NK, Narayan G: Genetic studies of Type 2 Diabetes mellitus in population of eastern Uttar Pradesh: Study of E23K polymorphism in KCNJ11 gene. International Conference on Genes, Genetics &Genomics: Today & Tomorrow- Human Concerns & XXXVII Annual conference of the Indian Society of Human Genetics, Panjab University, Chandigarh, India, Poster Abstract No. PP 068, Yadav SS, Prasad SB, Das M, Goverdhan HB, Pandey LK, Singh S, Pradhan S, Narayan G: SLIT2/ROBO1 signalling axis regulates the cancer promoting events through CXCR4/CXCL12 axis in cervical carcinogenesis. International conference on Genes, Genetics &Genomics: Today & Tomorrow- Human Concerns & XXXVII Annual conference of the Indian Society of Human Genetics, Panjab University, Chandigarh, India, Poster Abstract No. PP 172, Kumari S, Prasad SB, Yadav SS, Das M, Prakash S, Singh S, Puneet, Narayan G: COX-2 expression correlates with clinicopathology of gastric carcinoma. International conference on Genes, Genetics & Genomics: Today & Tomorrow- Human Concerns & XXXVII Annual conference of the Indian Society of Human Genetics, Panjab University, Chandigarh, India, Poster Abstract No. PP 173, Singh S, Prasad SB, Yadav SS, Agrawal NK, Narayan G: Association of genetic variants of CDKN2A/2B-rs and WFS1-rs loci with Type 2 Diabetes in populations of Eastern Uttar Pradesh, India. International conference on genomics, genetic diseases and diagnostics & XXXVI Annual conference of the Indian society of human genetics, Manipal University, Manipal, India, Abstract No. P Prasad SB, Yadav SS, Das M, Goverdhan HB, Pandey LK, Singh S, Pradhan S, Narayan G: Study of the role of PI3K/PTEN/AKT signaling pathway mediated regulation of the FOXO1 in cervical cancer. XXXIV All India Cell Biology Conference, Bose Institute, Kolkata, India, Poster Abstract No. 3, Yadav SS, Prasad SB, Das M, Goverdhan HB, Pandey LK, Singh S, Pradhan S, Narayan G: Chemokine receptor CXCR4 is epigenetically regulated in the cancer of cervix uteri. XXXIV All India Cell Biology Conference, Bose Institute, Kolkata, India, Poster Abstract No. 35, Yadav S, Prasad SB, Yadav SS, Agrawal NK, Narayan G, Singh S: Association of TCF7L2 rs (G/T) and PPARγ rs (C/G) gene polymorphism to Type 2 Diabetes mellitus in eastern Uttar Pradesh population. XXXIV All India Cell Biology Conference, Bose Institute, Kolkata, India, Poster Abstract No. 17, Das M, Prasad SB, Yadav SS, Goverdhan HB, Pandey LK, Singh S, Pradhan S, Narayan G: Comparative analysis of the mrna Expression of Minichromosome 7
8 maintenance genes in cervical cancer. XXXIV All India Cell Biology Conference, Bose Institute, Kolkata, India, Poster Abstract No. 33, Participated in Conferences/symposia: 2 nd International Symposium: Translational Cancer Research Cancer Research: From Bench to Bedside Organized by Delhi State Cancer Institute in collaboration with UT MD ADERSON CANCERCENTR Houston, USA held at New Delhi, 01 December A Symposium on Population Genetics and Chromatin dynamics organized by Cytogenetics Section, Department of Zoology, Banaras Hindu University, Varanasi, India. January 22-23, A Symposium DNA Repair, Genomics Instability and Cancer organized by Department of Zoology, Banaras Hindu University, Varanasi, India. March 4-5, International Conferences on Functional Genomics: Proteomics and Challenges organized by Cytogenetics Section, Department of Zoology, Banaras Hindu University, Varanasi, India. October 2-4, XXXII All India Cell Biology Conference (AICBC) & Symposia on Stem Cells and Pattern Formation organized by MACS-Agharkar Research Institute, Pune, India. December 4-6, National Conference on Genomics, Proteomics & System Biology at J.N. Tata Auditorium, IISc Campus, Bangalore. October 1-3 rd, XXXI All India Cell Biology Conference (AICBC) & Symposium on Stem Cells: Application and Prospects organized by Department of Zoology, Banaras Hindu University, Varanasi, India. December 14-16, 2007 TEACHING & RESEARCH EXPERIENCES: 1. Currently working as Assistant Professor in the Department of Microbiology at Central University of Rajasthan, (From Aug-2015 to till date). 2. ICMR-Post-Doctoral Fellow (ICMR-PDF), at Institute of Cytology and Preventive Oncology, Noida in ICMR awarded project entitled Role of GSK3β and STAT3 signaling in regulation of Epithelial Mesenchymal Transition in Cervical Cancer. (April-2014-Aug-2015). 3. ICMR-Senior Research Fellow (ICMR-SRF), in the Department of Molecular and Human Genetics (BHU), Varanasi in ICMR awarded project entitled Study of the PI3K/AKT signaling pathway mediated regulation of apoptotic and cell cycle associated genes in Cervical Cancer. (July April-2014). 4. DBT-Junior Research Fellow (DBT-JRF) in the Department of Molecular and Human Genetics (BHU), Varanasi in Department of Biotechnology Govt. of India 8
9 funded project entitled Molecular pathology of cervical carcinogenesis (Mar-2008 December 2011). 5. M.Sc. Dissertation in the Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India, Project entitled Study of antioxidant properties of different plant extract in CCl4- treated mononuclear cells isolated from chicken blood. (January 2006-June- 2006). TRAINING COURSE/WORKSHOP ATTENDED: 1. Attended one week National workshop on Mathematical Modelling and Simulation, organized by Department of Mathematics, Central University Rajasthan, India during March 14-18, A Workshop on Faculty Development Programme on Research and Data analysis using SPSS, Organized by Department of Statistics, SAP (DRS-I), DST-CIMS, Banaras Hindu University, India and SPSS South Asia during January, A Workshop on QPCR Methods, Applications and Technologies Organized by TATAA Biocenter, Sweden & Polyclone Bioservices, at JN Tata auditorium, IISc. Bangalore, India on 1 st October, MEMBERSHIP, CERTIFICATION AND AWARDS/SCHOLSHIP: 1. ICMR-Post-Doctoral Research Fellowship (ICMR-PDF), awarded by Indian Council of medical Research (I.C.M.R.), New Delhi, Govt. of India (March-2014). 2. ICMR-Senior Research Fellowship (ICMR-SRF), awarded by Indian Council of Medical Research (ICMR), New Delhi, Govt. of India (July-2012). 3. Qualified Graduate Aptitude Test for Engineering (GATE) with percentile (All India Rank-346, Score-405) (February-2005). 4. Postgraduate merit Scholarship was provided by Department of Biotechnology Government of India ( ). 5. Certificate Course in Yogic practices for Better Living, Yoga Sadhana Kendra, Banaras Hindu University (BHU), Varanasi, India. 6. Awarded with Silver medal for second position in 4x100 Meters Rely (Men). 7. Two years ( ) Membership for Indian society of Cell Biology Conference (AICBC). Membership for Indian Society of Human Genetics for the year
10 PERSONAL INFORMATION: Gender Male Language known English and Hindi Nationality Indian Marital status Married Date of birth 30 th November 1979 REFEREE: 1. Prof. Gopeshwar Narayan (Professor & Head) Department of Molecular and Human Genetics Banaras Hindu University Varanasi (UP) , India Phone: Prof. Satyajit Pradhan Professor Department of Radiotherapy & Radiation Medicine Institute of Medical Sciences Banaras Hindu University Varanasi (UP) , India Phone: Dr. Alok Chandra Bharti, Ph.D., INSA Young Scientist 2009, Professor, Molecular Oncology Laboratory, Department of Zoology, University of Delhi, Delhi , INDIA. M: s: Regards, (Dr. Shyam Babu Prasad) Assistant Professor Central University Rajasthan 10
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