TRAVIS JOHN ANTES, PHD CURRICULUM VITAE. (as of 3/09/16)

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1 TRAVIS JOHN ANTES, PHD CURRICULUM VITAE (as of 3/09/16) PERSONAL HISTORY: Business Address: 1092 Davis Building, 8700 Beverly Blvd, Los Angeles, CA Business Telephone: Business EDUCATION: 1991 BS, Biochemistry, Texas A&M University 1995 MS, Molecular and Cell Biology, University of Texas at Dallas 1998 PhD, Molecular and Cell Biology, University of Texas at Dallas FELLOWSHIP: 2001 Postdoctoral Fellowship completed at Stanford University, Department of Medicine PROFESSIONAL EXPERIENCE: 2016 Research Scientist II, Cedars-Sinai Medical Center, Heart Institute Senior Director, System Biosciences (SBI) Field Applications Specialist, Applied Biosystems, Inc Co-founder, Toccata Therapeutics, Inc Staff Scientist, Deltagen, Inc. PROFESSIONAL ACTIVITIES:

2 Consulting Activities: Scientific and technical consultant for Capricor Therapeutics, Inc. HONORS AND SPECIAL AWARDS: 1987 SPE Gulf Coast Section Undergraduate Scholarship Winner 1991 Awarded Texas A&M Cooperative Education Certificate of Completion 1996, 1997 University of Texas at Dallas Teaching Assistant of the Year Award RESEARCH GRANTS AND FELLOWSHIPS RECEIVED: Principal Investigator, Phase II NIH-SBIR Grant, 5R44GM , New Methods for Discovery and Study of MicroRNAs and other Small Noncoding RNAs Principal Investigator, Phase I NIH-SBIR Grant, 1R43GM , Permanent Knockdown of MicroRNAs using a Lentiviral-based Anti-miR System Co-Investigator, Phase I NIH-SBIR Grant, 2R44GM A1, Lentivectors engineered for expression of epitope-tagged microrna processing factors and PIWI factors to pull-down the proteins and their associated RNAs along with a novel technique for cloning small RNAs. Product line termed mir-snares for MicroRNA Discovery PATENTS RECEIVED: #US 9,005,888 B2, issued 4/15/2015, Method for Microvesicle Enrichment and Selective Removal. LECTURES AND PRESENTATIONS: 1. Insertional Editing of the Mitochondrial Large Subunit rrna of Physarum polycephalum, UTD, RNA 96 Research Conference, Madison, WI, Transcription Independent Cytidine Incorporation into RNA in Isolated Physarum Mitochondria", UTD, Gordon Research Conference on RNA Editing, Ventura, CA, 1997

3 3. In vivo Regulation of Intestinal and Hepatic Expression of the Human Apolipoprotein B Gene", Stanford, 21st Annual West Coast Chromatin and Chromosomes Conference, Asilomar, CA, "Key Regulatory Elements Involved in Intestinal and Hepatic Expression of the Human Apolipoprotein B Gene in Transgenic Mice", Stanford, The Kern Aspen Lipid Conference, Aspen, CO, "Identification of the Human Apolipoprotein B Gene Intestinal Transcriptional Enhancer using Transgenesis", Stanford, American Heart Association Conference, New Orleans, LA, "Permanent Knockdown of MicroRNAs using Lentivectors, SBI, Keystone Symposium: MicroRNA and Cancer, Keystone, CO, An exosome isolation system for serum-based cancer biomarker discovery, SBI, Cancer Research, American Association Of Cancer Research (AACR), Denver, CO, Methods for Studying MicroRNAs and Exosomes, SBI, City of Hope, Los Angeles, CA, Study and Analysis of MicroRNAs, SBI, Stanford University., Stanford, CA, Exosomes as MicroRNA Shuttles, SBI, AMGEN, Thousand Oaks, CA, Gene Delivery Technologies, SBI, U. Washington, Seattle, WA, Powerful Tools for MicroRNA Functional Analysis, SBI, Eisai Pharma, Tokyo, Japan, Exosomes & Microvesicles: Isolation, detection, biomarkers and engineering, SBI, MD Anderson, Houston, TX, Novel Tools for Programming Extracellular Vesicle Cargo for Delivery to Target Cells, SBI, ASEMV meeting, Marco Island, FL, Engineering Therapeutic Nanoshuttles, SBI, ASEMV meeting, Marco Island, FL, 2015 PUBLICATION/BIBLIOGRAPHY RESEARCH PAPERS

4 A. RESEARCH PAPERS - PEER REVIEWED 1. Antes T.J., Costandy H., Mahendran R., Spottswood M. and Miller, D., Insertional RNA Editing of Mitochondrial Transfer RNA in Physarum polycephalum and Didymium nigripes, (1998) Mol. Cell Biol. 18: Antes, T.J., Goodart, S.G., Huynh, C., Sullivan, M., Young, S.G. and Levy-Wilson, B., Identification and Characterization of a 315-bp Enhancer, Located more than 55 Kilobases 5 of the Apolipoprotein B Gene, that Confers Expression the Intestine, (2000) J. Biol Chem. 275: Antes, T.J., Chen, J., Cooper, A.D. and Levy-Wilson, B., The Nuclear Matrix Protein CDP Represses Hepatic Transcription of the Human Cholesterol 7alpha-Hydroxylase Gene, (2000) J. Biol Chem. 275: Levy-Wilson B., Paulweber B., Antes T.J., Goodart S.A., Lee S.Y., An Open Chromatin Structure in a Liver-specific Enhancer that Confers High Level Expression to Human Apolipoprotein B Transgenes in Mice, (2000) Mol Cell Biol Res Commun. 4 (4): Antes, T.J. and Levy-Wilson, B. HNF-3b, C/EBPb and HNF-4 Act in Synergy to Enhance Transcription of the Apolipoprotein B Gene in Intestinal Cells, (2001) Journal of DNA and Cell Biology. 20(2): Antes T.J., Goodart S.A., Chen W., Levy-Wilson B., Human apolipoprotein B gene intestinal control region, Biochemistry. (2001) 12;40(23): Antes, T.J., Namciu, S.J., Fournier, R.E., Levy-Wilson, B., The 5' Boundary of the Human Apolipoprotein B Chromatin Domain in Intestinal Cells, (2001) Biochemistry 40(23): Antes, T., Goodart, S., Huynh, C., Sullivan, M., Young, S. G., & Levy-Wilson, B., A 315-bp enhancer, located more than 55 KB 5 of the apolipoprotein B gene, confers intestinal expression in transgenic mice. Atherosclerosis, 151(1), Antes, T., Transcriptional repression of the human CYP7A1 gene by a nuclear matrix protein, Atherosclerosis, Volume 151, Issue 1,

5 10. Miller, L., Antes, T., Qian, F. and Miller, D.L., Identification of a putative mitochondrial RNA polymerase from Physarum polycephalum: characterization, expression, purification, and transcription in vitro,(2006) Current Genetics (10): Siebert, P., and Antes, T., Enrichment Amplification of Human RNase III-cleaved RNA Reveals New MicroRNAs and Other Small Non-Coding RNAs, (2007) PHILICA Article number Li X., Shen Y., Ichikawa H., Antes T., Goldberg G.S., Regulation of mirna expression by Src and contact normalization: effects on nonanchored cell growth and migration, (2009) Oncogene. 3;28(48): Juan D., Alexe G., Antes T., Liu H., Madabhushi A., Delisi C., Ganesan S., Bhanot G., Liou L.S., Identification of a MicroRNA Panel for Clear-Cell Kidney Cancer, (2010) Urology 75(4): Lesnik, J., and Antes, T., Permanent knockdown of micrornas using lentivectors, (2010) BioTechniques, Vol. 48, No. 4, pp Fong, M., Lesnik, J., Li, G., Antes, T., Lu, B., Cyto-Tracers: Novel lentiviral-based molecular imaging tools, (2010) BioTechniques; 49(5): Mendenhall A, Lesnik J, Mukherjee C, Antes T, Sengupta R., Packaging HIV- or FIV-based lentivector expression constructs and transduction of VSV-G pseudotyped viral particles, (2012) J Vis Exp; e3171. doi: / Uhde-Stone, C., Sarkar N., Antes T., Otoc N., Kim Y., Jiang YJ., Lu B., A TALEN-based strategy for efficient bi-allelic mirna ablation in human cells, (2014) RNA; 20(6): Peterson M.F., Otoc N., Sethi J.K., Gupta A., Antes T., Integrated systems for exosome investigation, (2015) Methods; pii: S (15) Lesnik, J., Antes, T., Kim, J., Griner, E., Pedro, L., Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET, (2016) elife; 5:e07383, 1-15.

6 ABSTRACTS 1. Antes T.J., Costandy H., Mahendran R., Spottswood M. and Miller, D., "Insertional Editing of the Mitochondrial Large Subunit rrna of Physarum polycephalum", RNA 96 Research Conference, 1996, Madison, Wisconsin. 2. Antes T.J., Costandy H., Mahendran R., Spottswood M. and Miller, D., "Transcription Independent Cytidine Incorporation into RNA in Isolated Physarum Mitochondria", Gordon Research Conference on RNA Editing, 1997, Ventura, California. 3. Antes, T.J., Goodart, S.G., Huynh, C., and Levy-Wilson, B., "In vivo Regulation of Intestinal and Hepatic Expression of the Human Apolipoprotein B Gene", 21st Annual West Coast Chromatin and Chromosomes Conference, 1999, Asilomar, California. 4. Antes, T.J., Goodart, S.G., Huynh, C., and Levy-Wilson, B., "Key Regulatory Elements Involved in Intestinal and Hepatic Expression of the Human Apolipoprotein B Gene in Transgenic Mice", The Kern Aspen Lipid Conference, 1999, Aspen, Colorado. 5. Antes, T.J., Goodart, S.G., Huynh, C., Sullivan, M., Young, S.G. and Levy-Wilson, B., "Identification of the Human Apolipoprotein B Gene Intestinal Transcriptional Enhancer using Transgenesis", American Heart Association Conference, 2000, New Orleans, Louisiana. 6. Antes, T., Jake Lesnik, J. and and Joan Wilson, J., Permanent Knockdown of MicroRNAs using Lentivectors, Keystone Symposium: MicroRNA and Cancer, 2009, Keystone, CO. 7. Wu, F. and Antes, T., An exosome isolation system for serum-based cancer biomarker discovery, Cancer Research (Impact Factor: 9.33). American Association Of Cancer Research (AACR), 2009, Denver, CO. 8. Antes, T., Isolation, Detection and Biomarker Discovery in Exosomes, Exosomes and Microvesicles Annual Meeting, 2012, Orlando, Florida. 9. Gupta, A., Afshari, A., Sethis, J., and Antes, T., Novel Tools for Labeling and Loading Biomolecules into Extracellular Vesicles, SBI, American Society for Exosomes and Microvesicles (ASEMV) meeting, 2015, Marco Island, Florida.

7 10. Antes, T., Engineering Therapeutic Nanoshuttles, American Society for Exosomes and Microvesicles (ASEMV) meeting, 2015, Marco Island, Florida.