Donald D. Ruhl, Jr., Ph.D.

Transcription

1 Department of Biochemistry and Molecular Biology Oklahoma State University 348F Noble Research Center Stillwater, Oklahoma Office: Fax: CURRENT POSITION Oklahoma State University, Stillwater, Oklahoma Assistant Professor, February 2011 to Current Academic Advisor Department of Biochemistry and Molecular Biology EDUCATION Cornell University, Ithaca, New York Postdoctoral Fellow, July 2005 to June 2010 Department of Molecular Biology and Genetics Principal Advisor: Dr. W. Lee Kraus Saint Louis University School of Medicine, Saint Louis, Missouri Doctor of Philosophy, July 2005 Department of Pharmacological and Physiological Sciences Principal Advisor: Dr. John C. Chrivia Thesis Title: Protein Kinase A, the Estrogen Response Element and a Chromatin Remodeling Complex: Two Mechanisms in Gene Regulation. University of Missouri Saint Louis, Saint Louis, Missouri Bachelor of Arts in Chemistry, December 1993 Principal Advisor: Dr. William Welsh Research studies in drug molecular modeling. RESEARCH EXPERIENCE Cornell University, Postdoctoral Fellow, July 2005 to June 2010 Study the regulation of chromatin structure and transcription of estrogen regulated genes by PARP-1 and Swi/Snf using biochemical, biophysical and cell-based approaches. Saint Louis University School of Medicine, Graduate Student, August 2000 to July 2005 Purified and identified the components of a SRCAP-containing protein complex and determined its role as a histone variant, H2A.Z, exchange complex using protein purification, biochemical and proteomic approaches.

2 Saint Louis University, Immunology Technician, March 1999 to August 2000 Performed PCR based assays for genetic analysis of clinical specimens. Specific tasks include isolating DNA, amplifying DNA by PCR, running electrophoretic gels and southern blots. Saint Louis University School of Medicine, Research Assistant, November 1997 to March 1999 Performed laboratory experiments for a basic science research project in which I tested small nucleolar RNA s in vivo for elements associated with the stability of these ribonucleotides, their localization into the nucleus, and their formation with ribonucleoprotein particles. Specific tasks included isolation of X. leavis oocytes, microinjection of oocytes, in vitro transcription reactions, and purification of RNA. BetzDearborn, Field Chemist, December 1994 to December 1997 Optimized and troubleshot chemical and biological water treatment programs. Specific tasks included setting up trial parameters, running the trials, performing wet chemistry tests, interpreting data, writing reports, and presenting the findings. Researched new chemical formulations for corrosion and scaling tendencies. Mallinckrodt Medical, Analytical Research Technician, August 1994 to November 1994 Method development project using primarily HPLC and GC instrumentation. Food and Drug Administration, Physical Science Technician, May 1993 to May 1994 Drug method research project for determining chlorhexidine and its degradation products. Primary tasks included running the HPLC instrumentation and preparing samples and solutions for analysis. TEACHING EXPERIENCE Oklahoma State University, Stillwater, Oklahoma Survey of Biochemistry, BIOC 3653, 3 credit hours. Fall Current Course is for upper level undergraduate students (approx. 165 students/fall semester) Biochemical Laboratory, BIOC 3723, 3 credit hours, Fall Current Course is for upper level undergraduate students (approx. 24 students/semester) Biochemical Laboratory Methods, BIOC 5824, 4 credit hours, Fall Current Course is for graduate students (1 student/semester) Teaching Internship, BIOC 4990, 3 credit hours, Fall Current Teaching experience for undergraduates (1 student/fall semester) Chemical Application of Biomolecules, BIOC 2344, 4 credit hours, Spring Current Course is for undergraduate non-majors (approx. 30 students/spring semester) Special Problems, BIOC 4990, variable credit hours, Spring 2011 Current Research experience for undergraduates (approx. 9 students/semester) 2

3 Cornell University, Ithaca, New York Undergraduate Research Mentor, January 2009 to May 2010 Student: Arunabha Sunny Basu. Sunny is investigating the role of PARP-1 in the inhibition of Swi/Snf chromatin remodeling. Lecture Title: Pharmacogenomics and Gene Therapy Lecture Title: Imprinting and Imprinting Related Diseases Lecture Title: Autoimmune Diseases Lecture Title: Polygenic Disease: Diabetes Guest Lecturer, Course: Molecular Basis of Human Disease, Fall 2008 Lecture Title: Hypermethylation of Tumor Suppressor Genes in Cancer Undergraduate Research Mentor, January 2006 to May 2008 Student: Ehsan Balagamwala. Ehsan s Senior Research Thesis, Biochemical Analyses of Poly(ADP-Ribose) Polymerase-1 Interactions and Functions on Chromatin Templates, received Highest Honors from Cornell University. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York Teaching Assistant, Gene Expression Course 2009 Taught biochemical methods for studying transcriptional regulation to graduate students and postdocs. (Total Students: 16) Teaching Assistant, Gene Expression Course 2008 Taught biochemical methods for studying transcriptional regulation to graduate students and postdocs. (Total Students: 16) Saint Louis University School of Medicine, Saint Louis, Missouri Undergraduate Research Mentor, January 2003 to May 2005 Student: Jennifer Jimenez. Jennifer studied the role of PKA in the regulation of ERE containing genes. Teaching Assistant, Physiology, Fall 2004 This course was part of a program designed to give disadvantaged students an opportunity to strengthen their application to medical school. (Total Students: 12) 3

4 Princeton Review, Saint Louis, Missouri Instructor, MCAT Chemistry, Spring 2002 Taught students material equivalent to the first two college semesters of General Chemistry. (Total Students: 40) PUBLICATIONS Ruhl DD, Forth S, Wang MD, Kraus WL. The Estrogen Receptor Modulates the Activity of Swi/Snf. (in preparation). Ruhl DD, Basu A, Kraus WL. Poly ADP-Ribose Inhibits the Remodeling Activity of Swi/Snf. (in preparation). Zhang T, Berrocal JG, Yao J, Dumond ME, Krishnakumar R, Ruhl DD, Ryu KW, Gamble MJ, Kraus WL Regulation of Poly(ADP-ribose) Polymerase-1-dependent Gene Expression through Promoter-directed Recruitment of a Nuclear NAD+ Synthase. J Biol Chem. 287: Langelier MF, Ruhl DD*, Planck JL, Kraus WL, Pascal JM The Zn3 Domain of Human Poly(ADP-Ribose) Polymerase-1 (PARP-1) Functions in Both DNA- dependent Poly(ADP-Ribose) Synthesis Activitiy and Chromatin Compaction. J Biol Chem. 285: * Co-first Author Hah N, Kolkman A, Ruhl DD, Pijnappel WW, Heck AJ, Timmers HT, Kraus WL A Role for BAF57 in Cell Cycle-Dependent Transcriptional Regulation by the SWI/SNF Chromatin Remodeling Complex. Cancer Research. 70: Ruhl DD, Kraus WL Biochemical Analyses of Nuclear Receptor-Dependent Transcription with Chromatin Templates. In: Chakravarti D (ed.), Regulatory Mechanisms in Transcriptional Signaling: Transcriptional and Physiological Control by Nuclear Hormone Receptors, (series: Progress in Molecular Biology and Translational Science, Volume 87) Elsevier, pp Wacker DA, Ruhl DD*, Balagamwala EH, Hope KM, Zhang T, Kraus WL The DNA Binding and Catalytic Domains of Poly(ADP-Ribose) Polymerase 1 Cooperate in the Regulation of Chromatin Structure and Transcription. Mol Cell Biol. 27: * Co-first Author Ruhl DD, Jin J, Cai Y, Swanson S, Florens L, Washburn MP, Conaway RC, Conaway JW, Chrivia JC Purification of a Human SRCAP Complex that Remodels Chromatin by Incorporating the Histone Variant H2A.Z into Nucleosomes. Biochemistry. 45: Monroy MA, Ruhl DD, Xu X, Granner DK, Yaciuk P, Chrivia JC Regulation of camp- Responsive Element-Binding Protein-Mediated Transcription by the SNF2/SWI-related Protein, SRCAP. Journal of Biological Chemistry. 276:

5 Ruhl DD, Pusateri ME, Eliceiri GL Multiple Conserved Segments of E1 Small Nucleolar RNA are Involved in the Formation of a Ribonucleoprotein Particle in Frog Oocytes. Biochemical Journal. 348: Doub WH, Ruhl DD, Hart B, Mehelic PR, Revelle LK Gradient Liquid Chromatographic Method for Determination of Chlorhexidine and its Degradation Products in Bulk Material. Journal of AOAC International. 79: ABSTRACTS/PRESENTATIONS AT NATIONAL CONFERENCES Ruhl DD, Forth S, Wang MD, Kraus WL Estrogen Receptor-Dependent Modulation of SWI/SNF Activity. Keystone Symposia. Gibson BA, Ruhl DD, Krishnakumar R, Basu A, Kraus WL Regulation of Chromatin Structure and Gene Expression by Poly(ADP-ribose) polymerase-1. Keystone Symposia. Ruhl DD, Kraus WL Estrogen Receptor-Dependent Modulation of SWI/SNF Activity. Keystone Symposia. Ruhl DD, Hah N, Kolkman A, Pijnappel P, Timmers MH, Heck A, Kraus WL Role of the Baf57 and Baf60a Subunits of Human SWI/SNF in Estrogen Receptor-mediated Gene Expression. FASEB. Ruhl DD, Hah N, Kraus WL Role of the SWI/SNF Chromatin Remodeling Complex in Estrogen Regulated Transcription. Keystone Symposia. Ruhl DD, Ruh MF, Chrivia JC Activation of the Estrogen Response Element by PKA Independent of the Estrogen Receptor. Endo Ruhl DD, Chrivia JC Transcriptional Activation by SRCAP, and the Purification of a SRCAP Complex. Endo Ruhl DD, Chrivia JC Activation of Estrogen Response Elements by Protein Kinase A. Jensen Symposium. Monroy MA, Ruhl DD, Yaciuk P, Chrivia JC SRCAP Enhances Glucocorticoid Receptor-Regulated Gene Expression. Endo Grosso LE, Ruhl D, Paterson A Analysis of Polymorphisms/Mutations in Coagulation Associated Proteins in a High Risk Obstetric Practice. Association for Molecular Pathology

6 GRANTS CASNR Student Tech Fee, 2011, Two Liquid Chromatography Units and a Computer. Awarded $19,587. FELLOWSHIPS Postdoctoral Fellowship. Recruitment and Activation of the Swi/Snf Chromatin Remodeling Complex at Estrogen Receptor-regulated Promoters in Breast Cancer Cells. NIH Training Grant, Department of Pharmacology, Weill Cornell Medical College. May 1, 2009 to April 30, Grant Number: 5T32 CA Postdoctoral Fellowship. NRSA-NIDDK. June, June, Reference Number: F32 DK (Declined) Postdoctoral Fellowship. American Heart Association Northwest Affiliate. July 1, 2006 to June 30, Reference Number: T. (Declined) Postdoctoral Fellowship. Recruitment and Activation of the SWI/SNF Chromatin Remodeling Complex at Estrogen Receptor-regulated Promoters in Breast Cancer Cells. Susan G. Komen Breast Cancer Foundation. May 1, 2006 to April 30, Grant Number: PDF Predoctoral Fellowship. NIH Training Grant, Department of Pharmacological and Physiological Science, Saint Louis University. July 1, 2004 to June 30, Grant number: 5T32GM Predoctoral Fellowship. Regulation of Estrogen Receptor Mediated Transcription by the SNF2- Related Protein, SRCAP. American Heart Association-Heartland Affiliate. July 1, 2002 to June 30, Grant Number: Z. AWARDS Keystone Symposia Scholarship, Estrogen Receptor-dependent Modulation of Swi/Snf Activity. First Place, Sigma Xi, Activation of the Estrogen Response Element by Protein Kinase A Independent of the Estrogen Receptor. Third Place, St. Louis University Graduate Student Research Symposium, Activation of the Estrogen Response Element by Protein Kinase A. 6