Figure S1. Cos-7. Cos-7 GFP. pjnk C2C12 C2C12 GFP * 60. pjnk. WT lamin A. H222P lamin A

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1 Figure S1 A Cos-7 C NT WT lamin A Cos-7 H222P lamin A 100 % JNK nucleus / positives GFP GFP pjnk ** NT D C2C12 NT WT lamin A GFP pjnk H222P lamin A C2C12 H222P lamin A 100 % JNK nucleus / positives GFP B WT lamin A 80 * NT WT lamin A H222P lamin A

2 Supplemental data Figure S1. Expression of H222P lamin A in transfected Cos-7 and C2C12 leads to enhanced nuclear translocation of phospho-jnk. (A-B) Effect of H222P lamin A on nuclear translocation of pjnk in transfected Cos-7 (A) and C2C12 (B) cells. Representative photomicrographs are shown for non-transfected cells (NT), transfected cells expressing a GFP fusion of wild type lamin A (WT lamin A) and transfected cells expressing a GFP fusion of lamin A with the H222P amino acid substitution (H222P lamin A). Arrowheads show enhanced nuclear localization of pjnk in cells expressing GFP-H222P lamin A. Bars: 10 m. (C-D) Percentages of Cos-7 (C) and C2C12 (D) cells with pjnk primarily in the nucleus. Non-transfected cells (NT), transfected cells expressing a GFP fusion of wild type lamin A (WT lamin A) and transfected cells expressing a GFP fusion of lamin A with the H222P aa substitution (H222P lamin A) were randomly counted and scored for nuclear pjnk (see arrowheads in A for example). Transfected cells were determined by presence of GFP signal. Values are means % standard deviations for n=200 cells per group (*p<0.05, **p<0.005).

3 Table S1. Genes with altered expression as defined by q<0.05 and >1 log 2 -fold change in hearts from Lmna H222P/+ mice. Probe set name Gene symbol Gene name fold q-value _at Myl7 myosin, light polypeptide 7, regulatory _at Sln sarcolipin _at Myl4 myosin, light polypeptide 4, alkali; atrial, embryonic _at Paip1 polyadenylate binding protein-interacting protein _at Myh7 myosin, heavy polypeptide 7, cardiac muscle, beta _at Prg4 proteoglycan _at Cacna1c calcium channel, voltage-dependent, L type, alpha 1C subunit _at Car3 carbonic anhydrase _at Serpina3n serine (or cysteine) proteinase inhibitor, clade A, member 3N _a_at Ugt1a6 UDP glycosyltransferase 1 family, polypeptide A _at Wdfy1 WD repeat and FYVE domain containing _at Pdlim3 PDZ and LIM domain _a_at Rex3 reduced expression _at Osbpl3 oxysterol binding protein-like _at Calr3 calreticulin _at A930025J12RIK RIKEN cdna A930025J12 gene _at C20RIK RIKEN cdna C20 gene _at Cap1 CAP, adenylate cyclase-associated protein 1 (yeast) _a_at Idb2 inhibitor of DNA binding _a_at Cnot7 CCR4-NOT transcription complex, subunit _at C19RIK RIKEN cdna C19 gene _s_at Gnai1 guanine nucleotide binding protein, alpha inhibiting _at H08RIK RIKEN cdna H08 gene _at Adn adipsin _at C3 complement component _at Rbp7 retinol binding protein 7, cellular _at Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide _x_at AV AV Mus musculus pancreas C57BL/6J adult Mus musculus _at AV AV RIKEN full-length enriched, adult male olfactory _s_at Ifi202b interferon activated gene 202B _at Gprk5 G protein-coupled receptor kinase _at Comp cartilage oligomeric matrix protein _a_at C130038G02RIK RIKEN cdna C130038G02 gene _at Acdc adipocyte, C1Q and collagen domain containing _s_at Pbx3 pre B-cell leukemia transcription factor _at Efemp1 epidermal growth factor-containing fibulin-like extracellular matrix protein _at Cxcl12 chemokine (C-X-C motif) ligand _at Fgl2 fibrinogen-like protein _a_at Csrp2 cysteine and glycine-rich protein _at AK unknown _at BC unknown _at Mmrn2 multimerin _at Osmr oncostatin M receptor _at Cyp2e1 cytochrome P450, family 2, subfamily e, polypeptide _at C730034d20rik RIKEN cdna C730034D20 gene _s_at Catnal1 catenin alpha-like _x_at Slitl2 Slit-like 2 (Drosophila) _a_at Nfia nuclear factor I/A _a_at BC unknown _x_at D6MIT97 DNA segment, Chr 6, Massachusetts Institute of Technology _at BC cdna sequence BC _at Son Son cell proliferation protein _a_at N18RIK RIKEN cdna N18 gene _at AV AV Mus musculus 18-day embryo C57BL/6J Mus musculus _at Serpine2 serine (or cysteine) proteinase inhibitor, clade E, member _at C330016O16RIK RIKEN cdna C330016O16 gene _x_at Plagl1 pleiomorphic adenoma gene-like _at Dkk3 dickkopf homolog 3 (Xenopus laevis) _s_at BC unknown _at BB BB RIKEN full-length enriched, B16 F10Y cells Mus _at Cp ceruloplasmin _at Slp sex-limited protein _x_at M09RIK RIKEN cdna M09 gene _a_at Nov nephroblastoma overexpressed gene _at Cfh complement component factor h

4 _at H2-T23 histocompatibility 2, T region locus _at Ahr aryl-hydrocarbon receptor _s_at Rhobtb3 Rho-related BTB domain containing _at Ednra endothelin receptor type A _a_at Sox4 SRY-box containing gene _at Deadc1 deaminase domain containing _a_at Fmo2 flavin containing monooxygenase _at Egr1 early growth response _at Gpx3 glutathione peroxidase _at Vcam1 vascular cell adhesion molecule _s_at Acp1 acid phosphatase 1, soluble _at Bambi BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) _at H18RIK RIKEN cdna H18 gene _at Ak unknown _a_at Glo1 glyoxalase _a_at Nedd4 neural precursor cell expressed, developmentally down-regulated gene _at G04RIK RIKEN cdna G04 gene _at H2-Q7 histocompatibility 2, Q region locus _at Fus fusion, derived from t(12;16) malignant liposarcoma (human) _at Dpep1 dipeptidase 1 (renal) _at Zfp297 zinc finger protein _x_at BC unknown _x_at Ddr1 discoidin domain receptor family, member _at Fhl1 four and a half LIM domains _x_at Wdr6 WD repeat domain _at Loc similar to 60S ribosomal protein L _a_at Abhd6 abhydrolase domain containing _at Av AV RIKEN full-length enriched, adult male colon Mus _x_at AV expressed sequence AV _a_at BC unknown _at Loc similar to sodium channel beta 4 subunit _a_at Alas2 aminolevulinic acid synthase 2, erythroid _a_at M12573 unknown _at M22RIK RIKEN cdna M22 gene _at Cxcl7 chemokine (C-X-C motif) ligand _at BB BB RIKEN full-length enriched, 0 day neonate eyeball _at Hrasls HRAS-like suppressor _s_at Pttg1 pituitary tumor-transforming _at Cyfip2 cytoplasmic FMR1 interacting protein

5 Table S2. Genes with altered expression as defined by q<0.05 and >1 log 2 -fold change in hearts from Lmna H222P/H222P mice. Probe set name Gene symbol Gene name fold q-value _at Myl7 myosin, light polypeptide 7, regulatory E _at Sln sarcolipin _at Myl4 myosin, light polypeptide 4, alkali; atrial, embryonic _at Myh7 myosin, heavy polypeptide 7, cardiac muscle, beta _at AK unknown _at Car3 carbonic anhydrase _s_at Ifi202b interferon activated gene 202B _a_at Rex3 reduced expression _at Paip1 polyadenylate binding protein-interacting protein _at Prg4 proteoglycan _at Anf atrial natriuretic factor _at Arvcf armadillo repeat gene deleted in velo-cardio-facial syndrome _at Serpina3n serine (or cysteine) proteinase inhibitor, clade A, member 3N _s_at Gnai1 guanine nucleotide binding protein, alpha inhibiting _at BC unknown _at Wdfy1 WD repeat and FYVE domain containing _a_at Sox4 SRY-box containing gene _at Dkk3 dickkopf homolog 3 (Xenopus laevis) _at Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide _a_at Col1a2 procollagen, type I, alpha _at Osbpl3 oxysterol binding protein-like _at BC cdna sequence BC _a_at Cnot7 CCR4-NOT transcription complex, subunit _a_at Csrp2 cysteine and glycine-rich protein _a_at C130038G02RIK RIKEN cdna C130038G02 gene _at BC cdna sequence BC _at Cxcl12 chemokine (C-X-C motif) ligand _x_at H2-Aa histocompatibility 2, class II antigen A, alpha _at Pdlim3 PDZ and LIM domain _at D830013H23RIK RIKEN cdna D830013H23 gene _at Fgl2 fibrinogen-like protein _a_at Wif1 Wnt inhibitory factor _at C730034D20RIK RIKEN cdna C730034D20 gene _at Igf1 insulin-like growth factor _a_at Idb2 inhibitor of DNA binding _at Egr1 early growth response _at Comp cartilage oligomeric matrix protein _at Serpine2 serine (or cysteine) proteinase inhibitor, clade E, member _x_at Plagl1 pleiomorphic adenoma gene-like _at Gpx3 glutathione peroxidase _at Dcn decorin _at Dbp D site albumin promoter binding protein _at Bambi BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) _a_at Ii Ia-associated invariant chain _at C20RIK RIKEN cdna C20 gene _a_at BC unknown _at BC unknown _at Vcam1 vascular cell adhesion molecule _x_at Vegfc vascular endothelial growth factor C _at C330016O16RIK RIKEN cdna C330016O16 gene _at Deadc1 deaminase domain containing _at Rtn4 reticulon _at Son Son cell proliferation protein _s_at Acta2 actin, alpha 2, smooth muscle, aorta _x_at K24RIK RIKEN cdna K24 gene _at Mglap matrix gamma-carboxyglutamate (gla) protein _at Mmp14 matrix metalloproteinase 14 (membrane-inserted) _s_at Pbx3 pre B-cell leukemia transcription factor _at O13RIK RIKEN cdna O13 gene _at Fzd2 frizzled homolog 2 (Drosophila) _s_at O10RIK RIKEN cdna O10 gene _at Rbp7 retinol binding protein 7, cellular _a_at Ifi203 interferon activated gene _at Etv1 ets variant gene _at E17RIK RIKEN cdna E17 gene

6 _a_at Nov nephroblastoma overexpressed gene _at Cap1 CAP, adenylate cyclase-associated protein 1 (yeast) _at C330023F11RIK RIKEN cdna C330023F11 gene _at AW expressed sequence AW _at Mmrn2 multimerin _at Masp1 mannan-binding lectin serine protease _at E030026I10RIK RIKEN cdna E030026I10 gene _at Ahr aryl-hydrocarbon receptor _at Peg3 paternally expressed _at Pdgfra platelet derived growth factor receptor, alpha polypeptide _at Ifi30 interferon gamma inducible protein _s_at H2-Ab1 histocompatibility 2, class II antigen A, beta _at AK unknown _at Gprk5 G protein-coupled receptor kinase _at Fhl1 four and a half LIM domains _at H2-Eb1 histocompatibility 2, class II antigen E beta _at BG mac09f12.x1 Soares mouse 3NbMS Mus musculus cdna clone _a_at Btg1 B-cell translocation gene 1, anti-proliferative _at H2-T23 histocompatibility 2, T region locus _at BC unknown _x_at Ddr1 discoidin domain receptor family, member _s_at Slc38a1 solute carrier family 38, member _at Esrrbl1 estrogen-related receptor beta like _at Cp ceruloplasmin _at Rbp1 retinol binding protein 1, cellular _x_at Mrvldc1 MARVEL (membrane-associating) domain containing _x_at Ap1s2 adaptor-related protein complex 1, sigma 2 subunit _at Gucy1a3 guanylate cyclase 1, soluble, alpha _at Frg1 FSHD region gene _at C05RIK RIKEN cdna C05 gene _at Loc similar to sodium channel beta 4 subunit _at BB BB RIKEN full-length enriched, 0 day neonate thymus _at AI UI-M-BG0-aht-a-04-0-UI.s1 NIH_BMAP_MSC Mus musculus cdna _at G12RIK RIKEN cdna G12 gene _a_at BC unknown _s_at Spna1 spectrin alpha _at Pex19 peroxisome biogenesis factor _at BC unknown _at H15RIK RIKEN cdna H15 gene _at Kcna5 potassium voltage-gated channel, shaker-related subfamily, member _a_at H23RIK RIKEN cdna H23 gene _x_at AV expressed sequence AV _x_at Tubgcp5 tubulin, gamma complex associated protein _at M12573 unknown _at Hrasls HRAS-like suppressor _at BB BB RIKEN full-length enriched, 0 day neonate eyeball _a_at Pttg1 pituitary tumor-transforming _at Cyfip2 cytoplasmic FMR1 interacting protein _at G02RIK RIKEN cdna G02 gene

7 Table S3. Genes commonly affected as defined by q<0.05 and >1 log 2 -fold change in hearts from Lmna H222P/H222P and Lmna H222P/+ mice. Lmna H222P/H222P Lmna H222P/+ Probe set name Gene symbol Gene name fold q-value fold q-value _at Myl7 myosin, light polypeptide 7, regulatory E _at Sln sarcolipin _at Myl4 myosin, light polypeptide 4, alkali; atrial, embryonic _at Myh7 myosin, heavy polypeptide 7, cardiac muscle, beta _at Car3 carbonic anhydrase _s_at Ifi202b interferon activated gene 202B _a_at Rex3 reduced expression _at Paip1 polyadenylate binding protein-interacting protein _at Prg4 proteoglycan _s_at Gnai1 guanine nucleotide binding protein, alpha inhibiting _at BC unknown _at Wdfy1 WD repeat and FYVE domain containing _a_at Sox4 SRY-box containing gene _at Dkk3 dickkopf homolog 3 (Xenopus laevis) _at Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide _at Osbpl3 oxysterol binding protein-like _a_at Cnot7 CCR4-NOT transcription complex, subunit _a_at Csrp2 cysteine and glycine-rich protein _a_at C130038G02RIK RIKEN cdna C130038G02 gene _at BC cdna sequence BC _at Cxcl12 chemokine (C-X-C motif) ligand _at Pdlim3 PDZ and LIM domain _at Fgl2 fibrinogen-like protein _at C730034D20RIK RIKEN cdna C730034D20 gene _a_at Idb2 inhibitor of DNA binding _at Egr1 early growth response _at Comp cartilage oligomeric matrix protein _at Serpine2 serine (or cysteine) proteinase inhibitor, clade E, member _x_at Plagl1 pleiomorphic adenoma gene-like _at Gpx3 glutathione peroxidase _at Bambi BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) _at C20RIK RIKEN cdna C20 gene _a_at BC unknown _at BC unknown _at Vcam1 vascular cell adhesion molecule _at C330016O16RIK RIKEN cdna C330016O16 gene _at Deadc1 deaminase domain containing _at Son Son cell proliferation protein _s_at Pbx3 pre B-cell leukemia transcription factor _at Rbp7 retinol binding protein 7, cellular _a_at Nov nephroblastoma overexpressed gene _at Cap1 CAP, adenylate cyclase-associated protein 1 (yeast) _at Mmrn2 multimerin _at Ahr aryl-hydrocarbon receptor _at Gprk5 G protein-coupled receptor kinase _at Fhl1 four and a half LIM domains _at H2-T23 histocompatibility 2, T region locus _x_at Ddr1 discoidin domain receptor family, member _at Cp ceruloplasmin _at Loc similar to sodium channel beta 4 subunit _a_at BC unknown _x_at AV expressed sequence AV _at M12573 unknown _at Hrasls HRAS-like suppressor _at BB BB RIKEN full-length enriched, 0 day neonate eyeball _a_at Pttg1 pituitary tumor-transforming _at Cyfip2 cytoplasmic FMR1 interacting protein

8 Table S4. Genes from MAPK pathways affected as defined by q<0.05 in hearts from Lmna H222P/H222P mice. Gene symbol Gene name q-value Tgfb2 transforming growth factor, beta E-07 Fgf9 fibroblast growth factor E-06 Mapk8 mitogen activated protein kinase E-05 Evi1 ecotropic viral integration site E-05 Pdgfra platelet derived growth factor receptor, alpha polypeptide 4.59E-05 Ddit3 DNA-damage inducible transcript E-05 Pdgfa platelet derived growth factor, alpha 1.61E-04 Ikbkg inhibitor of kappab kinase gamma 5.91E-04 Rap1b RAS related protein 1b 6.48E-04 Tgfbr2 transforming growth factor, beta receptor II 6.56E-04 Rasa1 RAS p21 protein activator E-04 Map3k7 mitogen activated protein kinase kinase kinase E-04 Fgf12 fibroblast growth factor E-04 Flnb filamin, beta Tgfbr1 transforming growth factor, beta receptor I Rasa2 RAS p21 protein activator Ppp3ca protein phosphatase 3, catalytic subunit, alpha isoform Stk4 serine/threonine kinase Tgfb3 transforming growth factor, beta Il1b interleukin 1 beta Stmn1 stathmin Dusp9 dual specificity phosphatase Mapk7 mitogen activated protein kinase Tnfrsf1a tumor necrosis factor receptor superfamily, member 1a Prkx protein kinase, X-linked Nfkb2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p Pla2g12b phospholipase A2, group XIIB Mapk14 mitogen activated protein kinase Arrb2 arrestin, beta Map3k7ip2 mitogen-activated protein kinase kinase kinase 7 interacting protein Fgf14 fibroblast growth factor Map2k4 mitogen activated protein kinase kinase Map3k4 mitogen activated protein kinase kinase kinase Rapgef4 Rap guanine nucleotide exchange factor (GEF) B230120H23Rik RIKEN cdna B230120H23 gene Fgfr1 fibroblast growth factor receptor Nfatc2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent Mapk1 mitogen activated protein kinase Casp3 caspase Atf2 activating transcription factor Pdgfrb platelet derived growth factor receptor, beta polypeptide Rasgrp1 RAS guanyl releasing protein Pla2g2f phospholipase A2, group IIF Map3k5 mitogen activated protein kinase kinase kinase Map4k4 mitogen-activated protein kinase kinase kinase kinase Ntf3 neurotrophin Prkacb protein kinase, camp dependent, catalytic, beta Fgf13 fibroblast growth factor Nras neuroblastoma ras oncogene Crk v-crk sarcoma virus CT10 oncogene homolog (avian) Cdc42 cell division cycle 42 homolog (S. cerevisiae) Mapk9 mitogen activated protein kinase Mef2c myocyte enhancer factor 2C Ikbkb inhibitor of kappab kinase beta Pak1 p21 (CDKN1A)-activated kinase Stk3 serine/threonine kinase 3 (Ste20, yeast homolog) Pla2g4a phospholipase A2, group IVA (cytosolic, calcium-dependent) Mapk8ip3 mitogen-activated protein kinase 8 interacting protein Ntrk2 neurotrophic tyrosine kinase, receptor, type Map2k1 mitogen activated protein kinase kinase Elk4 ELK4, member of ETS oncogene family Il1r2 interleukin 1 receptor, type II Ppm1a protein phosphatase 1A, magnesium dependent, alpha isoform

9 Elk1 ELK1, member of ETS oncogene family Map3k12 mitogen activated protein kinase kinase kinase Grb2 growth factor receptor bound protein Dusp4 dual specificity phosphatase Atf4 activating transcription factor Ptprr protein tyrosine phosphatase, receptor type, R Map3k14 mitogen-activated protein kinase kinase kinase Ppp3cb protein phosphatase 3, catalytic subunit, beta isoform Map4k3 mitogen-activated protein kinase kinase kinase kinase Map4k1 mitogen activated protein kinase kinase kinase kinase Mapkapk5 MAP kinase-activated protein kinase Tmem37 transmembrane protein Tnik TRAF2 and NCK interacting kinase Prkca protein kinase C, alpha Sos1 Son of sevenless homolog 1 (Drosophila) Hspa5 heat shock 70kD protein 5 (glucose-regulated protein) Ntrk1 neurotrophic tyrosine kinase, receptor, type Rps6ka4 ribosomal protein S6 kinase, polypeptide Srf serum response factor Pdgfb platelet derived growth factor, B polypeptide

10 Table S5. Genes from MAPK pathways affected as defined by q<0.05 in hearts from Lmna H222P/+ mice. Gene symbol Gene name q-value Fgf12 fibroblast growth factor E-06 Evi1 ecotropic viral integration site E-04 Raf1 v-raf-leukemia viral oncogene E-04 Tgfbr2 transforming growth factor, beta receptor II 2.29E-04 B230120H23Rik RIKEN cdna B230120H23 gene 2.45E-04 Ppm1a protein phosphatase 1A, magnesium dependent, alpha isoform 2.59E-04 Stmn1 stathmin E-04 Mapk8 mitogen activated protein kinase E-04 Fgf10 fibroblast growth factor E-04 Rasgrp1 RAS guanyl releasing protein E-04 Map3k7ip2 mitogen-activated protein kinase kinase kinase 7 interacting protein E-04 Pdgfra platelet derived growth factor receptor, alpha polypeptide 4.98E-04 Rap1b RAS related protein 1b 6.48E-04 Dusp9 dual specificity phosphatase E-04 Srf serum response factor 7.34E-04 Flnb filamin, beta 8.02E-04 Map3k5 mitogen activated protein kinase kinase kinase Pak1 p21 (CDKN1A)-activated kinase Tgfb2 transforming growth factor, beta Rasa2 RAS p21 protein activator Rasgrf1 RAS protein-specific guanine nucleotide-releasing factor Fgfr1 fibroblast growth factor receptor Mapk1 mitogen activated protein kinase Tnfrsf1a tumor necrosis factor receptor superfamily, member 1a Rasa1 RAS p21 protein activator Pdgfa platelet derived growth factor, alpha Stk4 serine/threonine kinase Map4k3 mitogen-activated protein kinase kinase kinase kinase Traf6 Tnf receptor-associated factor Map2k1 mitogen activated protein kinase kinase Ntrk2 neurotrophic tyrosine kinase, receptor, type Arrb2 arrestin, beta Map3k12 mitogen activated protein kinase kinase kinase Ppp3ca protein phosphatase 3, catalytic subunit, alpha isoform Ikbkb inhibitor of kappab kinase beta Fgf9 fibroblast growth factor Tgfbr1 transforming growth factor, beta receptor I Nf1 neurofibromatosis Il1r2 interleukin 1 receptor, type II Tmem37 transmembrane protein Fgfr4 fibroblast growth factor receptor Elk4 ELK4, member of ETS oncogene family Pla2g4a phospholipase A2, group IVA (cytosolic, calcium-dependent) Prkaca protein kinase, camp dependent, catalytic, alpha Dusp7 dual specificity phosphatase Map2k7 mitogen activated protein kinase kinase Atf4 activating transcription factor Stk3 serine/threonine kinase 3 (Ste20, yeast homolog) Casp4 caspase 4, apoptosis-related cysteine peptidase Map3k7 mitogen activated protein kinase kinase kinase Ptpn5 protein tyrosine phosphatase, non-receptor type Map3k14 mitogen-activated protein kinase kinase kinase Il1b interleukin 1 beta Egf epidermal growth factor Ikbkg inhibitor of kappab kinase gamma Map2k6 mitogen activated protein kinase kinase Map2k1ip1 mitogen-activated protein kinase kinase 1 interacting protein Acvr1b activin A receptor, type 1B Map4k4 mitogen-activated protein kinase kinase kinase kinase Rap1a RAS-related protein-1a Mapk9 mitogen activated protein kinase Map3k7ip1 mitogen-activated protein kinase kinase kinase 7 interacting protein

11 Map3k4 mitogen activated protein kinase kinase kinase Kras v-ki-ras2 Kirsten rat sarcoma viral oncogene homolog Casp14 caspase Dusp4 dual specificity phosphatase Casp1 caspase Nfatc2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent Rapgef4 Rap guanine nucleotide exchange factor (GEF) Nr4a1 nuclear receptor subfamily 4, group A, member Map3k3 mitogen activated protein kinase kinase kinase Hspa1l heat shock protein 1-like Ntf3 neurotrophin Fgf14 fibroblast growth factor Fasl Fas ligand (TNF superfamily, member 6) Mef2c myocyte enhancer factor 2C Ppp3cb protein phosphatase 3, catalytic subunit, beta isoform Fgf22 fibroblast growth factor Casp6 caspase Mknk1 MAP kinase-interacting serine/threonine kinase Sitpec signaling intermediate in Toll pathway-evolutionarily conserved Mapk13 mitogen activated protein kinase Fgfr2 fibroblast growth factor receptor Chuk conserved helix-loop-helix ubiquitous kinase Casp9 caspase Mknk2 MAP kinase-interacting serine/threonine kinase Fgf1 fibroblast growth factor Atf2 activating transcription factor Ikbke inhibitor of kappab kinase epsilon Akt3 thymoma viral proto-oncogene Pla2g12b phospholipase A2, group XIIB Prkcb1 protein kinase C, beta Nlk nemo like kinase Nfkb1 nuclear factor of kappa light chain gene enhancer in B-cells 1, p Ntf5 neurotrophin Ppm1b protein phosphatase 1B, magnesium dependent, beta isoform Pak2 p21 (CDKN1A)-activated kinase Ppp3r2 protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II) Ntrk1 neurotrophic tyrosine kinase, receptor, type Mapk8ip3 mitogen-activated protein kinase 8 interacting protein Daxx Fas death domain-associated protein Prkcc protein kinase C, gamma Mapkapk2 MAP kinase-activated protein kinase Crk v-crk sarcoma virus CT10 oncogene homolog (avian) Nras neuroblastoma ras oncogene Pla2g6 phospholipase A2, group VI Nfatc4 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent Fgf5 fibroblast growth factor Map4k2 mitogen activated protein kinase kinase kinase kinase Hspb1 heat shock protein Ptprr protein tyrosine phosphatase, receptor type, R

Table S1 Differentially expressed genes showing > 2 fold changes and p <0.01 for 0.01 mm NS-398, 0.1mM ibuprofen and COX-2 RNAi.

Table S1 Differentially expressed genes showing > 2 fold changes and p