Supplementary Table 1. Genes analysed for expression by angiogenesis gene-array.

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Prosper Strickland
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1 Supplementary Table 1. Genes analysed for expression by angiogenesis gene-array. Gene symbol Gene name TaqMan Assay ID UniGene ID 18S rrna 18S ribosomal RNA Hs _s1 Actb actin, beta Mm _s1 Mm , Mm , Mm Actb actin, beta Mm _g1 Mm , Mm , Mm Angpt1 angiopoietin 1 Mm _m1 Mm Angpt2 angiopoietin 2 Mm _m1 Mm Anpep alanyl (membrane) Mm _m1 Mm.4487 aminopeptidase Bai1 brain-specific Mm _m1 Mm angiogenesis inhibitor 1 Ccl11 chemokine (C-C motif) Mm _m1 Mm.4686 ligand 11 Ccl2 chemokine (C-C motif) Mm _m1 Mm ligand 2 Cdh5 cadherin 5 Mm _s1 Mm Col18a1 collagen, type XVIII, Mm _m1 Mm.4352 alpha 1 Col4a3 collagen, type IV, alpha Mm _m1 Mm Csf3 colony stimulating factor Mm _g1 Mm (granulocyte) Ctgf connective tissue growth Mm _g1 Mm factor Cxcl1 chemokine (C-X-C Mm _m1 Mm motif) ligand 1 Cxcl2 chemokine (C-X-C Mm _m1 Mm.4979 motif) ligand 2 Cxcl5 chemokine (C-X-C Mm _g1 Mm.4660 motif) ligand 5 Efna1 ephrin A1 Mm _m1 Mm Efnb2 ephrin B2 Mm _m1 Mm Egf epidermal growth factor Mm _m1 Mm Eng endoglin Mm _m1 Mm Epas1 endothelial PAS domain Mm _m1 Mm.1415 protein 1 Ephb4 Eph receptor B4 Mm _m1 Mm Ereg epiregulin Mm _m1 Mm.4791 F2 coagulation factor II Mm _m1 Mm Fgf1 fibroblast growth factor 1 Mm _s1 Mm Fgf2 fibroblast growth factor 2 Mm _m1 Mm Fgf6 fibroblast growth factor 6 Mm _m1 Mm.3403 Fgfr3 fibroblast growth factor Mm _s1 Mm.6904 receptor 3 Figf c-fos induced growth factor Mm _m1 Mm

2 Flt1 FMS-like tyrosine kinase Mm _m1 Mm Fzd5 frizzled homolog 5 (Drosophila) Mm _s1 Mm , Mm Gapdh glyceraldehyde-3- Mm _g1 Mm phosphate dehydrogenase Gna13 guanine nucleotide Mm _m1 Mm binding protein, alpha 13 Gusb glucuronidase, beta Mm _m1 Mm.3317 Gusb glucuronidase, beta Mm _s1 Mm.3317 Hand2 heart and neural crest Mm _m1 Mm derivatives expressed transcript 2 Hgf hepatocyte growth factor Mm _m1 Mm Hif1a hypoxia inducible factor 1, alpha subunit Mm _m1 Mm.3879, Mm Hprt1 hypoxanthine guanine Mm _m1 Mm phosphoribosyl transferase 1 Hsp90ab1 heat shock protein 90 Mm _g1 Mm.2180 alpha (cytosolic), class B member 1 Iapp islet amyloid polypeptide Mm _m1 Mm.415 Ifng interferon gamma Mm _m1 Mm Igf1 insulin-like growth factor Mm _m1 Mm Il1b interleukin 1 beta Mm _m1 Mm Il6 interleukin 6 Mm _m1 Mm.1019 Itgav integrin alpha V Mm _m1 Mm.227 Itgb3 integrin beta 3 Mm _m1 Mm Jag1 jagged 1 Mm _s1 Mm Kdr kinase insert domain Mm _m1 Mm.285 protein receptor Lama5 laminin, alpha 5 Mm _m1 Mm.4339 Lect1 leukocyte cell derived Mm _m1 Mm chemotaxin 1 Lep leptin Mm _m1 Mm Mapk14 mitogen-activated protein Mm _m1 Mm kinase 14 Mdk midkine Mm _m1 Mm.906 Mmp19 matrix metallopeptidase Mm _m1 Mm Mmp2 matrix metallopeptidase Mm _m1 Mm Mmp9 matrix metallopeptidase Mm _m1 Mm Npr1 natriuretic peptide Mm _m1 Mm.4627 receptor 1 Nrp1 neuropilin 1 Mm _m1 Mm , Mm Nrp2 neuropilin 2 Mm _m1 Mm Pdgfa platelet derived growth Mm _m1 Mm.2675

3 factor, alpha Pdx1 pancreatic and duodenal Mm _m1 Mm homeobox 1 Pecam1 platelet/endothelial cell Mm _m1 Mm adhesion molecule 1 Pgf placental growth factor Mm _m1 Mm.4809 Plau plasminogen activator, Mm _m1 Mm.4183 urokinase Plg plasminogen Mm _m1 Mm.971 Plg plasminogen Mm _m1 Mm.971 Ptgs1 prostaglandinendoperoxide Mm _m1 Mm synthase 1 S1pr1 sphingosine-1-phosphate Mm _m1 Mm.982 receptor 1 Serpinf1 serine (or cysteine) Mm _m1 Mm.2044 peptidase inhibitor, clade F, member 1 Smad5 MAD homolog 5 Mm _g1 Mm (Drosophila) Sphk1 sphingosine kinase 1 Mm _g1 Mm Stab1 stabilin 1 Mm _m1 Mm Tbp TATA box binding Mm _m1 Mm protein Tbp TATA box binding Mm _m1 Mm protein Tbx1 T-box 1 Mm _m1 Mm Tbx4 T-box 4 Mm _m1 Mm Tek endothelial-specific Mm _m1 Mm receptor tyrosine kinase Tgfa transforming growth Mm _m1 Mm factor alpha Tgfb1 transforming growth Mm _m1 Mm factor, beta 1 Tgfb2 transforming growth Mm _m1 Mm factor, beta 2 Tgfb3 transforming growth Mm _m1 Mm.3992 factor, beta 3 Tgfbr1 transforming growth factor, beta receptor I Mm _m1 Mm , Mm Thbs1 thrombospondin 1 Mm _m1 Mm.4159 Thbs2 thrombospondin 2 Mm _m1 Mm Timp1 tissue inhibitor of Mm _m1 Mm.8245 metalloproteinase 1 Timp2 tissue inhibitor of Mm _m1 Mm metalloproteinase 2 Tmprss6 transmembrane serine Mm _m1 Mm protease 6 Tnf tumor necrosis factor Mm _m1 Mm.1293 Tnfaip2 tumor necrosis factor, Mm _m1 Mm alpha-induced protein 2 Tnfsf12 tumor necrosis factor (ligand) superfamily, Mm _m1 Mm.8983

4 member 12 Tymp thymidine phosphorylase Mm _m1 Mm Vegfa vascular endothelial growth factor A Mm _m1 Mm , Mm Vegfb vascular endothelial Mm _g1 Mm growth factor B Vegfc vascular endothelial growth factor C Mm _m1 Mm.1402

5 Supplementary Table 2. Characteristics of islets for transplantation. Islet purity as assessed by dithizone (%) 1 day 4 days Intact Fragmented Intact 95±1 88±3* 100±0 Insulin release ng/10 islets x h Low glucose (1.67 mmol/l) High glucose (16.7 mmol/l) 15±3 34±6* 12±4 68±4 94±7* 72±6 All values are given as means ± SEM for 6-7 experiments in each group. * denotes P< 0.05 when compared to 1-day intact islets. Supplementary Figure 1. Representative images of Oil Red O staining of cryosections obtained from liver of high fat diet treated mice (positive control; A), and islet graft bearing liver (B), respectively. Arrow in B depicts islet. Scale bar is 100 µm.

B16F1 B16F10 Supplemental Figure S1

B16F1 B16F10 Supplemental Figure S1 B16F1 B16F1 Supplemental Figure S1 Representative microangiography images of B16F1 and B16F1 tumors grown in the cranial windows. FITC-dextran (2 million MW) was injected systemically to visualize blood