Untargeted metabolomics analysis reveals a link between ETHE1 mediated disruptive redox state and altered metabolic regulation
|
|
|
- Caroline Eaton
- 5 years ago
- Views:
Transcription
1 Supporting Information Untargeted metabolomics analysis reveals a link between ETHE1 mediated disruptive redox state and altered metabolic regulation Authors: Navid Sahebekhtiari 1*, Camilla Bak Nielsen 2, Mogens Johannsen 2 and Johan Palmfeldt 1* Affiliations: 1. Research Unit for Molecular Medicine, Department of Clinical Medicine, Aarhus University and Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, Denmark 2. Section for Forensic Chemistry, Department of Forensic Medicine, Aarhus University, Palle Juul-Jensens Boulevard 99, 82 Aarhus N, Denmark Figure S1: Extracted Ion Chromatogram (EIC) of identified metabolites with increased level in HDFs of ETHE1-deficient patients compared with controls. Figure S2: Extracted Ion Chromatogram (EIC) of identified metabolites with decreased level in HDFs of ETHE1-deficient patients compared with controls. Figure S3: Boxplots for identified metabolites which have significant alteration (p value <.5). Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; Spear - whiskers extend to minimum and maximum values (n=9). Boxplots were generated by BoxPlotR ( Figure S4: Fragmentation spectra for identified metabolites. Figure S5: Fragmentation spectra for unidentified features. Table S1: Characteristics of significantly altered features that escaped identification. Fragmentation spectra for these features were presented in Figure S4. S-1
2 Figure S1 β-citrylglutamate LysoPC 18: Controls Patients S-2
3 Figure S2 Pantothenic acid Pyroglutamic acid Glutathione S-Lactoylglutathione NAD+ NADH Controls Patients S-3
4 Malate Citrate Methionine Tryptophan Phenylalanine Tyrosine Glutamate methylester Controls Patients Glycerophosphoryl choline S-4
5 Lactate GSH dimer S-5
6 Fig S3 Beta citryl glutamate Lyso PC 18: S-6
7 Tyrosine Citrate Glutamate methylester S-7
8 S-8 Glycerophosphoryl choline GSH dimer Lactate
9 S-9 Malate Methionine NAD+
10 S-1 NADH Pantothenic acid Phenylalanine
11 S-11 Pyroglutamic acid S-Lactoylglutathione Tryptophan
12 Fig S4 S-Lactoylglutathione 2eV N1_3_frag19_1-A,3_1_3144.d: +MS2(38.184), 2.eV, 152.5s # S-lactoylglutathione_frag_2-E,4_1_334.d: +MS2( ), 2.eV, 142.s # m/z β-citryl-l-glutamic acid 2 ev 1 MMF2_3_frag12_2836.d: +MS2( ), 2.eV, 76.7s # betacitrylglatamicacid_frag_2-e,5_1_3348.d: +MS2( ), 2.eV, 76.7s # m/z S-12
13 glutamate methylester 2 ev N1_2_frag11_2835.d: +MS2( ), 2.eV, 37.9s # glutamicacid 5-metylester_frag_2-E,1_1_3349.d: +MS2( ), 2.eV, 38.s # m/z L-A-glycerophosphorylcholine 2 ev MMF2_3_frag12_2836.d: +MS2( ), 2.eV, 21.7s # glycerophosphoryl choline_frag_2-e,2_1_3338.d: +MS2( ), 2.eV, 23.s # m/z S-13
14 Pyroglutamic acid 1 ev N1_2_frag11_2835.d: +MS2(13.499), 1.eV, 59.7s # pyroglutamic acid_frag1_2ev_2-b,3_1_6961.d: +MS2(13.499), 1.eV, 55.5s # m/z Methionine 2 ev 1 N1_2_frag11_2835.d: +MS2(15.575), 2.eV, 44.9s # aa-mix_2-b,4_1_4124.d: +MS2(15.58), 2.eV, 46.7s # m/z S-14
15 Tyrosine 2eV N1_2_frag15_1-A,2_1_2839.d: +MS2( ), 2.eV, 66.8s # aa-mix_2-b,4_1_4124.d: +MS2( ), 2.eV, 71.s # m/z Phenylalanine 1eV N1_2_frag15_1-A,2_1_2839.d: +MS2( ), 1.eV, 187.8s # aa-mix_2-b,4_1_4124.d: +MS2( ), 1.eV, 189.5s # m/z S-15
16 Tryptophan 2eV 1 N1_2_frag15_1-A,2_1_2839.d: +MS2(25.947), 2.eV, 216.8s # aa-mix_2-b,4_1_4124.d: +MS2(25.16), 2.eV, 217.3s # m/z Glutathione red. 2eV N1_2_frag15_1-A,2_1_2839.d: +MS2(38.915), 2.eV, 48.s # glutathione_2-b,2_1_4126.d: +MS2(38.92), 2.eV, 47.9s # m/z S-16
17 Pantothenic acid 2eV N1_2_frag11_2835.d: +MS2( ), 2.eV, 28.s # pantothenic acid_2-b,5_1_4128.d: +MS2( ), 2.eV, 27.2s # m/z Citrate 2eV Navid control frag_neg_2-b,2_1_3362.d: -MS2( ), 2.eV, 5.9s # Apparatkontrol_frag citric_2-a,3_1_43.d: -MS2( ), 2.eV, 49.4s # m/z S-17
18 Malate 2eV 1 Navid control frag_neg_2-b,2_1_3362.d: -MS2( ), 2.eV, 31.9s # malic acid frag_2-d,1_1_444.d: -MS2( ), 2.eV, 31.7s # m/z lysopc 18: 2eV 1 MMF frag17_2-c,2_1_4312.d: +MS2( ), 2.eV, 562.8s # PC 18 frag_2-c,1_1_4311.d: +MS2( ), 2.eV, 563.6s # m/z S-18
19 NAD+ 2eV N7 frag NAD_2-B,3_1_4391.d: +MS2( ), 2.eV, 79.2s # NAD+ frag_2-b,2_1_4387.d: +MS2( ), 2.eV, 79.2s # m/z NADH 2eV 1 N1_2_frag11_2835.d: +MS2( ), 2.eV, 187.s # NADH frag_2-b,1_1_4454.d: +MS2( ), 2.eV, 185.5s # m/z S-19
20 Lactate 1 ev lactate_frag_control_2-b,2_1_4563.d: -MS2(89.249), 1.eV, 4.2s # lactate_frag_2-b,1_1_4562.d: -MS2(89.26), 1.eV, 4.2s # m/z S-2
21 Fig S5 M578T patient_frag22_2-b,2_1_456.d: +MS2( ), 1.eV, 32.7s # patient_frag22_2-b,2_1_456.d: +MS2( ), 2.eV, 32.9s # patient_frag22_2-b,2_1_456.d: +MS2( ), 4.eV, 33.2s # m/z M55T patient_frag22_2-b,2_1_455.d: +MS2( ), 1.eV, 32.7s # patient_frag22_2-b,2_1_455.d: +MS2( ), 2.eV, 32.9s # patient_frag22_2-b,2_1_455.d: +MS2( ), 4.eV, 33.2s # m/z S-21
22 M48T patient_frag22_2-b,2_1_456.d: +MS2( ), 1.eV, 496.1s # patient_frag22_2-b,2_1_456.d: +MS2(48.311), 2.eV, 496.4s # patient_frag22_2-b,2_1_456.d: +MS2(48.346), 4.eV, 496.6s # m/z M13T control_frag21_2-b,1_1_454.d: +MS2(13.491), 1.eV, 68.5s # control_frag21_2-b,1_1_454.d: +MS2( ), 2.eV, 68.8s # control_frag21_2-b,1_1_454.d: +MS2(13.487), 4.eV, 69.s # m/z S-22
23 M367T control_frag21_2-b,1_1_454.d: +MS2( ), 1.eV, 191.1s # control_frag21_2-b,1_1_454.d: +MS2( ), 2.eV, 191.3s # control_frag21_2-b,1_1_454.d: +MS2( ), 4.eV, 191.6s # m/z M61T577 1 MMF2_1_frag14_2838.d: +MS2( ), 1.eV, 559.2s # MMF2_1_frag14_2838.d: +MS2( ), 2.eV, 559.4s # MMF2_1_frag14_2838.d: +MS2( ), 4.eV, 559.7s # m/z S-23
24 M53T MMF2_1_frag13_2837.d: +MS2( ), 1.eV, 52.9s # MMF2_1_frag13_2837.d: +MS2( ), 2.eV, 53.1s # MMF2_1_frag13_2837.d: +MS2( ), 4.eV, 53.4s # m/z M558T MMF2_1_frag13_2837.d: +MS2( ), 1.eV, 529.1s # MMF2_1_frag13_2837.d: +MS2( ), 2.eV, 529.4s # MMF2_1_frag13_2837.d: +MS2( ), 4.eV, 529.6s # m/z S-24
25 M54T75 1 QC Navid_2-B,2_1_6962.d: -MS2(54.518), 1.eV, 69.s # QC Navid_2-B,2_1_6962.d: -MS2(54.515), 2.eV, 69.3s # QC Navid_2-B,2_1_6962.d: -MS2(54.534), 4.eV, 69.5s # m/z S-25
26 Table S1 name m/z value retention time [s] p-value FDR VIP-score ratio ionization M456T326 a positive M367T positive M54T negative M13T positive M578T positive M55T positive M48T positive M61T positive M53T positive M558T positive a : With regard to low intensity the fragmentation spectra is not available. S-26
Quantitative analysis of hydrophilic metabolite using ion-paring chromatography with a high-speed triple quadrupole mass spectrometer
Quantitative analysis of hydrophilic metabolite using ion-paring chromatography with a high-speed triple ASMS 2012 ThP11-251 Zanariah Hashim 1 ; Yudai Dempo1; Tairo Ogura 2 ; Ichiro Hirano 2 ; Junko Iida
Supplementary material
Supplementary material Comprehensive Characterization and Evaluation for Hepatocellular Carcinoma by LC-MS based Serum Metabolomics Journal: Metabolomics Xin Lu 1,3, Huan Nie 1, Yiqun Li 1, Chao Zhan 2,
Use of RapidFire/MS for Metabolomics. - from untargeted to targeted and Pathway driven analysis. RapidFire/MS. Moritz Wagner Agilent Technologies
Use of RapidFire/MS for Metabolomics RapidFire/MS - from untargeted to targeted and Pathway driven analysis Moritz Wagner Agilent Technologies 1 Agilent RapidFire TM High-throughput Mass Spectrometry System
Untargeted plasma and tissue metabolomics in rats with chronic kidney disease given AST-120.
Untargeted plasma and tissue metabolomics in rats with chronic kidney disease given AST-0. Thomas J Velenosi, Anzel Hennop, David A Feere, Alvin Tieu, Andrew S Kucey, Polydoros Kyriacou, Laura E McCuaig,
Table 1. Gas chromatographic and mass spectrometric data of the identified hydrophilic
Table 1. Gas chromatographic and mass spectrometric data of the identified hydrophilic compounds. Compound RT a RRT b RI c Quantification ion d Other characteristic ions Amino acids Alanine 9.109 0.499
SUPPLEMENTARY DATA Supplementary Figure 1. Body weight and fat mass of AdicerKO mice.
SUPPLEMENTARY DATA Supplementary Figure 1. Body weight and fat mass of AdicerKO mice. Twelve week old mice were subjected to ad libitum (AL) or dietary restriction (DR) regimens for three months. (A) Body
Supplementary materials
Supplementary materials Metabolic phenotyping for monitoring ovarian cancer patients Chaofu Ke a, Ang Li a, Yan Hou a, Meng Sun b, Kai Yang a, Jinlong Cheng c, Jingtao Wang a, Tingting Ge c, Fan Zhang
Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors
Analytical and Bioanalytical Chemistry Electronic Supplementary Material Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors Zhicheng Liu, Peiyuan Yin,
Metabolite identification in metabolomics: Database and interpretation of MSMS spectra
Metabolite identification in metabolomics: Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu utline Introduction Putative
Profiling and Quantitation of Metabolomic Signatures for Breast Cancer Cell Progression
Profiling and Quantitation of Metabolomic Signatures for Breast Cancer Cell Progression Henry Shion, 1 Irwin Kurland, 2 Sumanta Goswami, 2,3 David Kusin, 3 Haitao Lu, 2 Bhavapriya Vaitheesvaran, 2 John
William Wikoff : UC Davis (Committee chair) Pavel Aronov: Stanford University. John Asara: Harvard University. Vladimir Shulaev: University of Texas
MRG: Metabolomics Research Group William Wikoff : UC Davis (Committee chair) Pavel Aronov: Stanford University John Asara: Harvard University it Vladimir Shulaev: University of Texas Chris Turck: Max Planck
Metabolomics approach reveals metabolic disorders and potential. biomarkers associated with the developmental toxicity of
Supplementary information for Metabolomics approach reveals metabolic disorders and potential biomarkers associated with the developmental toxicity of tetrabromobisphenol A and tetrachlorobisphenol A Guozhu
Indolepropionic acid and novel lipid metabolites are associated with a lower. risk of type 2 diabetes in the Finnish Diabetes Prevention Study
1 Supplementary Material Indolepropionic acid and novel lipid metabolites are associated with a lower risk of type 2 diabetes in the Finnish Diabetes Prevention Study Vanessa D de Mello, Jussi Paananen,
Accurate determination of protein methionine oxidation by stable isotope labeling and LC-MS analysis
Accurate determination of protein methionine oxidation by stable isotope labeling and LC-MS analysis Hongcheng Liu*, Gomathinayagam Ponniah, Alyssa Neil, Rekha Patel, Bruce Andrien Protein Characterization,
Identification of Lipidomic Biomarkers for Coexposure to Subtoxic Doses of Benzo[a]pyrene and Cadmium: The Toxicological Cascade Biomarker Approach
![Identification of Lipidomic Biomarkers for Coexposure to Subtoxic Doses of Benzo[a]pyrene and Cadmium: The Toxicological Cascade Biomarker Approach](/thumbs/86/94000882.jpg "Identification of Lipidomic Biomarkers for Coexposure to Subtoxic Doses of Benzo[a]pyrene and Cadmium: The Toxicological Cascade Biomarker Approach")
Supporting Information Identification of Lipidomic Biomarkers for Coexposure to Subtoxic Doses of Benzo[a]pyrene and Cadmium: The Toxicological Cascade Biomarker Approach Harald Jungnickel,*,1 Sarah Potratz,
Supporting Information. New Insights into the Cytotoxic Mechanism of. Hexabromocyclododecane from a Metabolomic Approach
Supporting Information New Insights into the Cytotoxic Mechanism of Hexabromocyclododecane from a Metabolomic Approach Feidi Wang,, Haijun Zhang, *, Ningbo Geng,, Baoqin Zhang, Xiaoqian Ren,, Jiping Chen
Chem 109 C. Fall Armen Zakarian Office: Chemistry Bldn 2217
Chem 109 C Fall 2014 Armen Zakarian ffice: Chemistry Bldn 2217 Chapter 25 o Glycolysis : fates of pyruvate NADH, H + H + C 2 NADH, H + H - (S)-lactic acid lactate dehydrogenase; anaerobic conditions -
Large-Scale Metabolite Analysis by Laser Desorption Ionization Mass Spectrometry from Silicon Nanopost Arrays
Supporting Information Large-Scale Metabolite Analysis by Laser Desorption Ionization Mass Spectrometry from Silicon Nanopost Arrays Andrew R. Korte, Sylwia A. Stopka, Nicholas Morris, Trust Razunguzwa,
Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography
Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography Application Note Metabolomics Author Yuqin Dai Agilent Technologies, Inc. 31 Stevens Creek Blvd,
Introduction to Peptide Sequencing
Introduction to Peptide equencing Quadrupole Ion Traps tructural Biophysics Course December 3, 2014 12/8/14 Introduction to Peptide equencing - athan Yates 1 Why are ion traps used to sequence peptides?
Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra
Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu Outline Introduction
NMR ANNOTATION. Rémi Servien Patrick Tardivel Marie Tremblay-Franco Cécile Canlet 01/06/2017 v 1.0.0
NMR ANNOTATION Rémi Servien Patrick Tardivel Marie Tremblay-Franco Cécile Canlet 01/06/2017 v 1.0.0 WHY DO WE NEED AN AUTOMATED NMR ANNOTATION TOOL 2 Metabolomic workflow Control mice Exposed mice Metabolites
Effects of Histidine Supplementation on Global Serum and Urine 1 H NMR-based. Metabolomics and Serum Amino Acid Profiles in Obese Women from a
Supporting information for Effects of Histidine Supplementation on Global Serum and Urine 1 H NMR-based Metabolomics and Serum Amino Acid Profiles in Obese Women from a Randomized Controlled Study Shanshan
Supplementary Information. Effects of Perfluorooctanoic Acid on Metabolic Profiles in Brain and Liver of Mouse by a
Supplementary Information Effects of Perfluorooctanoic Acid on Metabolic Profiles in Brain and Liver of Mouse by a High-throughput Targeted Metabolomics Approach Nanyang Yu, Si Wei, *, Meiying Li, Jingping
Level and activity of D-amino acids in mouse brain tissue and blood
1 2 3 4 5 6 7 8 9 10 11 12 13 14 SUPPLEMENTARY INFORMATION Level and activity of D-amino acids in mouse brain tissue and blood Choyce A. Weatherly 1, Siqi Du 1, Curran Parpia 1, Polan T. Santos 2, Adam
The use of mass spectrometry in lipidomics. Outlines
The use of mass spectrometry in lipidomics Jeevan Prasain jprasain@uab.edu 6-2612 utlines Brief introduction to lipidomics Analytical methodology: MS/MS structure elucidation of phospholipids Phospholipid
AbsoluteIDQ Kit: Targeted Metabolomics Identification and Quantification
AbsoluteIDQ Kit: Targeted Metabolomics Identification and Quantification Accurate, Reproducible, Metabolomics Studies The word metabolome refers to the complete set of small molecule metabolites arising
FRAMINGHAM HEART STUDY CODING MANUAL Metabolomics (Hilic - installment 1)
FRAMINGHAM HEART STUDY CODING MANUAL Metabolomics (Hilic - installment 1) COHORT: Offspring TIME INTERVAL: Exam 5 DATASET NAME: l_mtbli1_ex05_1_0610s # OBSERVATIONS: 1075 High throughput metabolite profiling
Pyruvate Alanine 0.15 *** ** ***
SUPPLEMENTARY FIGURES Glucose ΔµM from fresh media / mg protein -1-2 -3 - -.1 -.3 -.5 Lactate Alanine Formate ΔµM from fresh media / mg protein 5 3 2 1.15.1.5.6..2.. NS-3 WT-NS G93A-NS Supplementary Figure
MS/MS Scan Modes. Eötvös University, Budapest April 16, MS/MS Scan Modes. Árpád Somogyi. Product Ion Scan Select. Scan. Precursor Ion Scan Scan
MS/MS Modes Árpád Somogyi Eötvös University, Budapest April 16, 2012 MS/MS Modes Product Ion Precursor Ion Neutral Loss Δ ed Reaction Monitoring (SRM) 1 modes in a triple quadrupole (QqQ) (one quadrupole
Metabolism Gluconeogenesis/Citric Acid Cycle
Metabolism Gluconeogenesis/Citric Acid Cycle BIOB111 CHEMISTRY & BIOCHEMISTRY Session 21 Session Plan Gluconeogenesis Cori Cycle Common Metabolic Pathway The Citric Acid Cycle Stoker 2014, p859 Gluconeogenesis
METABOLIC VULNERABILITIES OF CANCER. Eyal Gottlieb
METABOLIC VULNERABILITIES OF CANCER Eyal Gottlieb METABOLIC VULNERABILITIES OF CANCER Eyal Gottlieb Cancer and metabolism: the anabolic angle glucose glucose-6-phosphate Ribose-5-phosphate ADP + Pi Serine
A Design Automation Framework for Computational Bioenergetics in Biological Networks
This journal is The Royal Society of Chemistry 3 A Design Automation Framework for Computational Bioenergetics in Biological Networks Claudio Angione, a Jole Costanza, b Giovanni Carapezza, a Pietro Lió
FREE ENERGY Reactions involving free energy: 1. Exergonic 2. Endergonic
BIOENERGETICS FREE ENERGY It is the portion of the total energy change in a system that is available for doing work at constant temperature and pressure; it is represented as ΔG. Reactions involving free
Proteins consist in whole or large part of amino acids. Simple proteins consist only of amino acids.
Today we begin our discussion of the structure and properties of proteins. Proteins consist in whole or large part of amino acids. Simple proteins consist only of amino acids. Conjugated proteins contain
Citric acid cycle and respiratory chain. Pavla Balínová
Citric acid cycle and respiratory chain Pavla Balínová Mitochondria Structure of mitochondria: Outer membrane Inner membrane (folded) Matrix space (mtdna, ribosomes, enzymes of CAC, β-oxidation of FA,
NOVEL METABOLIC FEATURES IN ACINETOBACTER BAYLYI ADP1 REVEALED BY A MULTIOMICS APPROACH
Supplementary materials for manuscript NOVEL METABOLIC FEATURES IN ACINETOBACTER BAYLYI ADP1 REVEALED BY A MULTIOMICS APPROACH 1-3 Lucille Stuani, 1-3 Christophe Lechaplais, 1-4 Aaro V. Salminen, 1-3 Béatrice
Fate of Dietary Protein
Fate of Dietary Protein Dietary protein Stomach: l, pepsin Denatured and partially hydrolyzed protein (large polypeptides) small intestine: proteases Amino acids and dipeptides intestinal lining: proteases
Nuclear Magnetic Resonance (NMR) Spectroscopy
Nuclear Magnetic Resonance (NMR) Spectroscopy G. A. Nagana Gowda Northwest Metabolomics Research Center University of Washington, Seattle, WA 1D NMR Blood Based Metabolomics Histidine Histidine Formate
T1-1 Table 1. Target compounds and individual food additives.
T1-1 Table 1. Target compounds and individual food additives. No. 1 Aspartame Sweetener Aspartame D 1 1 2 Xylitol Sweetener Xylitol D 2 2 3 Glycyrrhizine Sweetener Disodium glycyrrhizinate D 3 3 Licorice
Amino Acid Metabolism
Amino Acid Metabolism Fate of Dietary Protein Dietary protein Stomach: l, pepsin Denatured and partially hydrolyzed protein (large polypeptides) small intestine: proteases Amino acids and dipeptides intestinal
The citric acid cycle Sitruunahappokierto Citronsyracykeln
The citric acid cycle Sitruunahappokierto Citronsyracykeln Ove Eriksson BLL/Biokemia ove.eriksson@helsinki.fi Metabolome: The complete set of small-molecule metabolites to be found in a cell or an organism.
BIOL 158: BIOLOGICAL CHEMISTRY II
BIOL 158: BIOLOGICAL CHEMISTRY II Lecture 5: Vitamins and Coenzymes Lecturer: Christopher Larbie, PhD Introduction Cofactors bind to the active site and assist in the reaction mechanism Apoenzyme is an
OIL ON CHINOOK SALMON SMOLTS
TOXIC EFFECTS OF CRUDE AND DISPERSED OIL ON CHINOOK SALMON SMOLTS (ONCORHYNCHUS TSHAWYTSCHA) Ronald S. Tjeerdema, Ching-Yu Lin, Brian S. Anderson Department of Environmental Toxicology University of California,
Sample Preparation is Key
PLOS ONE DOI: 10.1371/journal.pone.0117232 February 6, 2015 Presented by Katie Gibbs Sample Preparation is Key Sample extraction and instrumental analysis methods are well documented in metabolomics. Understanding
Metabolism and metabolomics in studying human health and ageing
Metabolism and metabolomics in studying human health and ageing Dr Warwick (Rick) Dunn School of Biosciences, Phenome Centre Birmingham and Institute of Metabolism and Systems Research, University of Birmingham,
Order Number: TEST Age: 6 Sex: F MRN: TESTGIRL3. Test Test MD Test Test, NC Zillicoa Street Asheville, NC Genova Diagnostics
63 Zillicoa Street Asheville, NC 2881 Genova Diagnostics Patient: GIRL3 TEST Age: 6 Sex: F MRN: TESTGIRL3 Order Number: 752837 Completed: March 28, 26 Received: March 28, 26 Collected: March 28, 26 Test
METABOLOMICS AS A TOOL TO STUDY DISEASE PROGRESSION
METABOLOMICS AS A TOOL TO STUDY DISEASE PROGRESSION Nagireddy Putluri, Ph.D. Associate Professor Director Metabolomics core Department of Molecular and Cellular Biology Alkek Center for Molecular Discovery
Supplementary Information. for
Supplementary Information for Serum Metabolomics to Identify the Liver Disease-Specific Biomarkers for the Progression of Hepatitis to Hepatocellular Carcinoma Rong Gao, 1 Jianhua Cheng, 1 Chunlei Fan
Metabolomic fingerprinting of serum samples by direct infusion mass spectrometry
Metabolomic fingerprinting of serum samples by direct infusion mass spectrometry Raúl González-Domínguez * Department of Chemistry, Faculty of Experimental Sciences. University of Huelva, Spain. * Corresponding
1 Which pathway for aerobic cellular respiration is located in the cytoplasm of the cell?
1 Which pathway for aerobic cellular respiration is located in the cytoplasm of the cell? glycolysis citric cycle 2 Which of the following statements is NOT correct regarding aerobic cellular respiration?
(A) Urea cycle (B) TCA cycle (C) Glycolysis (D) Pyruvate oxidation (E) Respiratory chain
Biochemistry - Problem Drill 15: Citric Acid Cycle No. 1 of 10 1. Which of the following statements is not a metabolic pathway involved in carbohydrate catabolism and ATP production. (A) Urea cycle (B)
METABOLOMIC ANALYSIS OF THE SECRETOME OF HUMAN EMBRYONIC STEM CELLS FOLLOWING METHYL PARATHION AND METHYL PARAOXON EXPOSURE
ECBC-TR-1178 METABOLOMIC ANALYSIS OF THE SECRETOME OF HUMAN EMBRYONIC STEM CELLS FOLLOWING METHYL PARATHION AND METHYL PARAOXON EXPOSURE PHASE : METABOLITE DOWNSELECTION FOR STRUCTURAL CONFIRMATION Janna
Supporting information for. Time-dependent Responses of Earthworms to Soil. Contaminated with Low Levels of Lead as Detected using 1 H
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2017 Supporting information for Time-dependent Responses of Earthworms to Soil Contaminated with
SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids
SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids Data Independent Acquisition on TripleTOF and X-Series QTOF Systems for Seized Drug Analysis Oscar G. Cabrices
Cornstarch
Electronic Supplementary Material (ESI) for Food & Function. This journal is The Royal Society of Chemistry 2018 Supplementary data : Supplementary Table 1: Diet composition (g/kg) on the basis of the
Metabolomic Data Analysis with MetaboAnalystR
Metabolomic Data Analysis with MetaboAnalystR Name: guest623839008349489450 February 7, 2018 1 Background Understanding the functional importance of metabolites in untargeted metabolomics is limited due
Jose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford, MA, U.S. and Manchester, UK
HIGH-THRUGHPUT REACTIVE METABLITE SCREEIG FR DICLFEAC BY UPLC AD XEV TQ MS WITH SCAWAVE Jose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford,
Metabolomic Data Analysis with MetaboAnalyst
Metabolomic Data Analysis with MetaboAnalyst User ID: guest4965371694680211620 April 14, 2009 1 Data Processing and Normalization 1.1 Reading and Processing the Raw Data MetaboAnalyst accepts a variety
NAS Workshop on the Industrialization of Biology
High throughput chemical characterization using mass spectrometry NAS Workshop on the Industrialization of Biology Jonathan V. Sweedler University of Illinois Urbana, IL USA May 2014 Funding: DOE, NSF
Ahmad Ulnar. Faisal Nimri ... Dr.Faisal
24 Ahmad Ulnar Faisal Nimri... Dr.Faisal Fatty Acid Synthesis - Occurs mainly in the Liver (to store excess carbohydrates as triacylglycerols(fat)) and in lactating mammary glands (for the production of
DART MSI of drugs of abuse in hair
DART MSI of drugs of abuse in hair Wilco Duvivier, Teris van Beek, Michel Nielen October 5, 2017 Forensic hair evidence Forensically interesting: Many compounds accumulate in hair Prolonged detectability
UHPLC-ESI-QTOF-MS/MS based Lipidomics by Data-Independent Acquisition
11.5.2016, 13:15, 15 + 5 min UHPLC-ESI-QTF-MS/MS based Lipidomics by Data-Independent Acquisition Michael Lämmerhofer, Jörg Schlotterbeck Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis,
Tomoko Tanahashi 1, Yasushi Tsuchihashi 3*, Akiyo K. Sakamiya 1 1, 2. and Takeo Yano
Metabolomic approach using ultra-high-performance liquid chromatography-mass spectrometry: Quantitative amino acid analysis and characterization of four species of marine fish, and a cuttlefish Tomoko
Electronic supplementary material to: The influence of L-carnitine on the formation of preneoplastic and
Electronic supplementary material to: The influence of L-carnitine on the formation of preneoplastic and atherosclerotic lesions in the colon and aorta of male F344 rats Archives of Toxicology Michael
Releasing Chemical Energy
Releasing Chemical Energy Ø Energy From Carbohydrates Ø Aerobic Respiration/ Stages Ø Fermentation Ø Food as a Source of Energy How Do Cells Access the Chemical Energy in Carbohydrayes? Aerobic Respiration
High Performance Gas Chromatography Mass Spectrometry in Addressing the Challenges of Metabolomic Studies Separation in Time and Mass
High Performance Gas Chromatography Mass Spectrometry in Addressing the Challenges of Metabolomic Studies Separation in Time and Mass Jeffrey S. Patrick Director of Marketed Technology LECO Separation
0091 Organix Comprehensive Profile Summary of abnormal results:
Ordering Physician: 255252-279741 Via Borin 48 31100 Treviso Number: Age: 43 Sex: F Date of Birth: 03/01/1964 Date Collected: 12/12/07 Date Received: 12/17/07 Telephone: Fax: Summary of abnormal results:
Amino Acid Metabolism
Amino Acid Metabolism Last Week Most of the Animal Kingdom = Lazy - Most higher organisms in the animal kindom don t bother to make all of the amino acids. - Instead, we eat things that make the essential
Amino Acids. Amino Acids. Fundamentals. While their name implies that amino acids are compounds that contain an NH. 3 and CO NH 3
Fundamentals While their name implies that amino acids are compounds that contain an 2 group and a 2 group, these groups are actually present as 3 and 2 respectively. They are classified as α, β, γ, etc..
Sulfur fertilization influences the sulphur species composition in Allium sativum: sulfomics using HPLC-ICP-MS/MS-ESI-MS/MS
Electronic upplementary Material (EI) for Metallomics. This journal is The Royal ociety of Chemistry 2017 Electronic supplement for ulfur fertilization influences the sulphur species composition in Allium
Supporting information for. Determination of sub nm levels of low molecular mass (LMM) thiols in natural waters by liquid
Supporting information for Determination of sub nm levels of low molecular mass (LMM) thiols in natural waters by liquid chromatography tandem mass spectrometry after derivatization with p-(hydroxymercuri)
III. 6. Test. Respiració cel lular
III. 6. Test. Respiració cel lular Chapter Questions 1) What is the term for metabolic pathways that release stored energy by breaking down complex molecules? A) anabolic pathways B) catabolic pathways
Respiration. Energy is everything!
Respiration Energy is everything! Tesla was incredible Everyone was intrigued by Tesla Tesla showed that energy does not need to be feared So what does this have to do with twinkies? Everything! Cellular
0291 Organix Basic Profile Summary of abnormal results:
Ordering Physician: 250200-274226 Reference Number: Age: 57 Sex: F Date of Birth: 12/25/1949 Date Collected: 11/18/07 Date Received: 11/18/07 Report Date: Telephone: Fax: Reprinted: 12/5/07 7704465483
A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS.
A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. Martin R Green*, Keith Richardson, John Chipperfield, Nick Tomczyk, Martin
[ APPLICATION NOTE ] UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research INTRODUCTION APPLICATION BENEFITS
![[ APPLICATION NOTE ] UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research INTRODUCTION APPLICATION BENEFITS](/thumbs/88/117895443.jpg "[ APPLICATION NOTE ] UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research INTRODUCTION APPLICATION BENEFITS")
UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research Padhraic Rossiter, 1 Jaime Salcedo Dominguez, 1 Jennifer Warren, 1 Norma Breen, 1 Lisa Calton 2 1 Waters Corporation,
High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring Buprenorphine and Norbuprenorphine in Urine
High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring and in Urine Xiaolei Xie, Joe DiBussolo, Marta Kozak; Thermo Fisher Scientific, San Jose, CA Application Note 627 Key Words, norbuprenorphine,
Diana Álvarez Muñoz, Albert Serra Compte, Natàlia Corcoll, Belinda Huerta, Sara Rodríguez Mozaz, Sergi Sabater, Damià Barceló.
Diana Álvarez Muñoz, Albert Serra Compte, Natàlia Corcoll, Belinda Huerta, Sara Rodríguez Mozaz, Sergi Sabater, Damià Barceló. INTRODUCTION MATERIAL AND METHODS Experimental Analytical determination Signal
Mass Spectrometry based metabolomics
Mass Spectrometry based metabolomics Metabolomics- A realm of small molecules (
CELLULAR RESPIRATION SUMMARY EQUATION. C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION
CELLULAR RESPIRATION SUMMARY EQUATION C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION Oxidation: partial or complete loss of electrons Reduction: partial or complete gain of electrons
Supporting Information
Supporting Information Chaleckis et al. 10.1073/pnas.1603023113 SI Materials and Methods Human Subject Characteristics. Thirty healthy male and female volunteers participated in this study (Table S2).
Inhibitory Effect of Methotrexate on Rheumatoid Arthritis Inflammation and Comprehensive Metabolomics Analysis Using UPLC-Q/TOF-MS
SUPPLEMENTARY MATERIALS Inhibitory Effect of Methotrexate on Rheumatoid Arthritis Inflammation and Comprehensive Metabolomics Analysis Using UPLC-Q/TOF-MS Zhiqiang Pang 1, Guoqiang Wang 1, Nan Ran 1, Hongqiang
Supplementary files. Tissue metabolomics study to reveal the toxicity of a Traditional Tibetan. medicines Renqing Changjue in rat
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2018 Supplementary files Tissue metabolomics study to reveal the toxicity of a Traditional Tibetan
How to Use TOF and Q-TOF Mass Spectrometers
How to Use TOF and Q-TOF Mass Spectrometers October 2011 What do TOF and Q-TOF offer? TOF Fast scanning of full spectrum High resolution full scan spectra Accurate mass measurements Q-TOF Fast scanning
Differences in elongation of very long chain fatty acids and fatty acid metabolism between triple-negative and hormone receptor-positive breast cancer
Yamashita et al. BMC Cancer (2017) 17:589 DOI 10.1186/s12885-017-3554-4 RESEARCH ARTICLE Open Access Differences in elongation of very long chain fatty acids and fatty acid metabolism between triple-negative
Journal of Cell Science Supplementary information. Arl8b +/- Arl8b -/- Inset B. electron density. genotype
J. Cell Sci. : doi:.4/jcs.59: Supplementary information E9. A Arl8b /- Arl8b -/- Arl8b Arl8b non-specific band Gapdh Tbp E7.5 HE Inset B D Control al am hf C E Arl8b -/- al am hf E8.5 F low middle high
Coenzymes, vitamins and trace elements 209. Petr Tůma Eva Samcová
Coenzymes, vitamins and trace elements 209 Petr Tůma Eva Samcová History and nomenclature of enzymes 1810, Gay-Lussac made an experiment with yeats alter saccharide to ethanol and CO 2 Fermentation From
Kazusa DNA Res Inst. Daisuke Shibata
Workshop Argentina-Japan Bioscience and Biotechnology for the promotion of Agricutulure and Food Production August 3 rd to 7 th 2009- Metabolite Metabolomics Databases approaches for Plant for Agro-Biotechnology
0410 Triad Bloodspot Profile
Accession Number: Order Number: Reference Number: Patient: Age: Date of Birth: Date Collected: Report Date: Telephone: Fax: Reprinted: Comment: 0410 Triad Bloodspot Profile This report contains the following:
University of Guelph Department of Chemistry and Biochemistry Structure and Function In Biochemistry
University of Guelph Department of Chemistry and Biochemistry 19-356 Structure and Function In Biochemistry Final Exam, April 21, 1997. Time allowed, 120 min. Answer questions 1-30 on the computer scoring
Low-level environmental phthalate exposure associates with urine metabolome. alteration in a Chinese male cohort
Supporting Information Low-level environmental phthalate exposure associates with urine metabolome alteration in a Chinese male cohort Jie Zhang*, Liangpo Liu, Xiaofei Wang, Qingyu Huang, Meiping Tian,
Notes CELLULAR RESPIRATION SUMMARY EQUATION C 6 H 12 O 6 + O 2. 6CO 2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION
AP BIOLOGY CELLULAR ENERGETICS ACTIVITY #2 Notes NAME DATE HOUR SUMMARY EQUATION CELLULAR RESPIRATION C 6 H 12 O 6 + O 2 6CO 2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION Oxidation: partial or complete
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents
Coversheet. Publication metadata
Coversheet This is the accepted manuscript (post-print version) of the article. Contentwise, the accepted manuscript version is identical to the final published version, but there may be differences in
Metabolomic and Proteomics Solutions for Integrated Biology. Christine Miller Omics Market Manager ASMS 2015
Metabolomic and Proteomics Solutions for Integrated Biology Christine Miller Omics Market Manager ASMS 2015 Integrating Biological Analysis Using Pathways Protein A R HO R Protein B Protein X Identifies
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nature22359 Supplementary Discussion KRAS and regulate central carbon metabolism, including pathways supplied by abundant fuels like glucose and glutamine. Under control
Chem 109 C. Fall Armen Zakarian Office: Chemistry Bldn 2217
Chem 109 C Fall 2014 Armen Zakarian ffice: Chemistry Bldn 2217 o Catabolism of carbohydrates: 10 reactions of glycolysis Chapter 25 C C 2 C 2 D-glucose α-d-glucopyranose aworth projection α-d-glucopyranose
Send questions to: prope mg.com
Send questions to: BPI.Seminars@propelmg.com Advancing Cell Culture-Based Biopharmaceutical Programs using Metabolomics Metabolomics in Bioprocessing Activities Cell line development/clone selection Targets