CENTER FOR INTERNATIONAL PROGRAMS

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1 CENTER FOR INTERNATIONAL PROGRAMS Course name: Conservation Biology of Sharks and Rays Course code: ENV 3160S Total Hours: 10 on line hours, 30 lecture hours, 50 hours of field lectures and field practice sessions Professor: Sebastián Hernández (Ph.D. Cell & Molecular BioScience) 1 week on line May 29 th June2, weeks in Costa Rica with lectures and field sessions June 4 th June 24 th, TENTATIVE PROGRAM SCHEDULE Day 1 SUN Activities: Welcome to COSTA RICA Airport Pick up Day 2 MON Activity: Departure to El Jobo (5 Days) 7:00 am Breakfast (with Host family in San Jose) 8:00 am Departure to El Jobo On the way: Field session overview 12:00 pm Lunch on the way to El Jobo 2:00-4:00 pm Review Syllabus & fieldtrip activities at El Jobo 6:00 pm Dinner (Kembly s house) 7:00 pm Presentation Equipo Tora Carey Day 3 TUE Activities: Snorkeling with rays & Class 7:30 am Snorkeling to El Jobo beach to recognize rays and practice sampling design to quantify rays and tissue samples collection Recollection of rubbish in water at El Jobo beach 2:00-6:00 pm Snorkeling to La cornuda beach to recognize rays and practice sampling design to quantify rays and tissue samples collection Recollection of rubbish in water at Manzanillo beach

2 Day 4 WED Activities: Snorkeling and sampling rays & Class (Kembly s house) 7:30 am Quantifying and determining size composition of ray and tissue samples collection at El Jobo beach (Snorkeling & BRUVs) 2:00-5:00 pm Lecture and hand on activity. Unit I: Biology of Elasmobranchs (taxonomy, external morphology and internal anatomy, and feeding system and habitats) 7:30 pm Movie Shark water I Day 5 THU Activities: Snorkeling and sampling rays & Class (Kembly s house) 7:30 pm Quantifying and determining size composition of ray and tissue samples collection at La Cornuda (Snorkeling & BRUVs) 2:00-5:00 pm Lecture and hand on activity. Unit I: Biology of Elasmobranchs (Nervous and sensorial systems, Reproductive biology) 7:30 pm Sea Turtle nesting ground tour 10:00 pm Resting time Day 6 FRI Activity: Departure from El Jobo to Universidad Veritas (Kembly s house) 12:00 pm Lunch (on the way back to San Jose) 2:00 pm Arriving to Universidad Veritas onward Free time and settling at host family Day 7 SAT Activities: Lecture and Lab (BIOMOL) 8:00 am 8:30 am Quick campus tour - Unit I: Biology of Elasmobranchs (Migration patterns, threats, managing and conservation issues) 1:30-6:30 pm Organize and analyze the information of rays sampled from field trip to El Jobo (Tissue sample collection, Snorkeling & BRUVs) Submission of data set and analyses for fieldtrip I 8:00 p.m. Optional tropical dance classes on campus til 9:30 (take (Shuttle pick at shuttle service back to homestay after class) Day 8 SUN Activities: Lecture and Lab (BIOMOL)

3 8:00 am Unit II: Basics in Genetics, remembering high school!!!!!! 12:00 pm Pizza lunch! 1:30-6:30 pm Unit II: Basics in genetic approaches for identification of elasmobranch species Lab activity: Knowing the lab equipment, pipetting exercises Day 9 MON Activities: Lecture and Lab (BIOMOL) 8:00 am Unit II: DNA extractions, PCRs and Visualization of PCRs products 12:00 pm Lunch at Universidad Veritas 1:30-6:30 pm Unit II: Bioinformatics of DNA sequences (GenBank, polymorphisms, design of primers species-specific) Lab activity: Preparing the tissue samples for DNA Extraction 8:00 p.m. Optional tropical dance classes on campus til 9:30 (take (Shuttle pick at shuttle service back to homestay after class) Day 10 TUE Activity: Lab (BIOMOL) 8:00 a.m. Lab activity: DNA Extraction 12:00 pm Lunch at Universidad Veritas 1:30-6:30 pm Lab activity: PCRs & Visualization of PCRs products Day 11 WED Activity: Departure to Reserva PlayaTortuga (5 Days) 8:00 am Departure to Reserva PlayaTortuga On the way: Field session overview 2:00-4:00 pm Review of fieldtrip activities 4:00-5:00 pm Presentation of Reserva Playa Tortuga projects Day 12 THU Activities: Visiting the local fishery community of Coronado (Reserva PlayaTortuga) 7:30 am Departure to local fishery community of Coronado 8:00 am Talks from local fishery community 9:30 am Sampling fish species from fisherman / Rubbish collection 2:00 pm Sampling fish species from fisherman / Rubbish collection

4 Day 13 FRI Activities: Visiting the local fishery community of Punta Mala (Reserva PlayaTortuga) 7:30 am Departure to local fishery community of Punta Mala 8:00 am Talks from local fishery community 9:30 am Sampling fish species from fisherman / Rubbish collection 2:00 pm Sampling fish species from fisherman / Rubbish collection Day 14 SAT Activities: Visiting the Fish Receivers or Recibidores of sharks products in Ciudad Cortes & Dominicalito 7:30 am Departure to Ciudad Cortes 8:00 am Visiting Fish Receivers or Recibidores 1:00 pm Lunch 2:00 pm Return to Reserva Playa Tortuga 3:00-5:00 pm Organizing the information and tissue samples obtained Day 15 SUN Activities: Fieldtrip to Isla del Caño 7:30 am Departure to Isla del Caño 9:00 am Snorkeling activity (Recognizing of elasmobranch species, BRUVs, tissue sample collection) 12:00 pm Lunch 1:00 pm Visiting Isla del Caño National Park (talks from park rangers) 3:00 pm Snorkeling activity (Recognizing of elasmobranch species, BRUVs, tissue sample collection) 5:00 pm Returning to Reserva Playa Tortuga Day 16 MON Departure from Reserva Playa Tortuga to Universidad Veritas (San Jose) 12:00 pm Lunch (on the way back to San Jose) 2:00 pm Arriving to Universidad Veritas 3:00-6:00 pm Preparation and submission of data set from fieldtrip II 6:00 p.m. Back to host family and dinner 8:00 p.m. (Shuttle pick at Optional tropical dance classes on campus til 9:30 (take shuttle service back to homestay after class)

5 Day 17 TUE Activities: Lecture and Lab (BIOMOL) 8:00 am Unit III: Basics in Population Genetics, approaches for conservation purposes 1:30-6:30 pm Bioinformatics laboratory (interpretation of chromatogram, editing DNA sequences, identification of polymorphics) Day 18 THU Activities: Lecture and Lab (BIOMOL) 8:00 am Unit III: Indexes of genetic diversity and interpretation of data 1:30-6:30 pm Bioinformatics laboratory to study genetic diversity Defining research topics for Student Research Presentations Day 19 FRI Activities: Lecture and Lab (BIOMOL) 8:00 am Morning research for Research presentations 1:30-6:30 pm Working on Student Research Presentations Day 20 SAT Activity: Student Research Presentation 8:00 am Research Presentations at the Biomol lab 12:00 pm Lunch Pizza day! 1:30-6:30 pm Finalizing Research Presentations 6:30 Farewell party with a Chilean style BBQ Day 21 SUN Activities: Departure back home Airport Drop off

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