(,, ) microrna(mirna) 19~25 nt RNA, RNA mirna mirna,, ;, mirna mirna. : microrna (mirna); ; ; ; : R321.1 : A : X(2015)

Transcription

1 35 1 Vol.35 No Jan Reproduction & Contraception doi: /j.issn X randc_journal@163.com microrna ( ) microrna() 19~25 nt RNA RNA ; : microrna (); ; ; ; : R321.1 : A : X(2015) %~15% %~20% 50% [12] 75% [2] [3-6] microrna() ( ) : ; Tel: ; Fax: ; syp2008@vip.sina.com 1 19~25 nt RNA RNA 3' (3'- untranslated regions 3'-UTR) RNA RNA Drosha Exportin- 5 (exportin-5-dependent) [78] s RNaseIII (Dicer) [9-11] (mirisc) 3'-UTR ; 1993 Lee (C. elegans) 37

2 [12] lin-4 7 Reinhart 2 let-7 : RNA RNA 20 RNA RNA microrna() 20 60%~75% 3 s [13] 30% 2 [14] [3] : [4] [15-20] [51521] Dicer Gdnf Kitl Man2a2 Serpina5 Dicer s 141 s : Y X X 2005 Yu [17] mir-469 mir-34 mir-16 mir-122a mir-470 mir-201c mir-143a mir-30b mir- 27 mir-15b mir-21 mir-471 mir-465 mir-101 mir-100 mir Yan [22] Luo [23] ; let7 mir-125a mir-9 2 [5] mir-125a let-7 let-7 Fas RAS [24] let-7 mir-125a mir-9 38

3 LIN28 LIN28 RNA 2009 [13] (mir-299 mir- 376a mir-381 mir-409-5p mir-67) Dicer 5 80% Dicer Dicer 2013 Wu [25] 128 [26] X mmu-mir-351 X X [1626] 4 [6] Hu ( ) [19] (45.9%) X X 15% X Lian 3 (non-obstructive azoospermia NOA) 2 [20] NOA RT-PCR mir-302a mir-491-3p mir- 520d-3p mir-383 NOA X 14q q13.42 X NOA mir- 17~92 mir NOA Yang [18] qrt-pcr (gene ontology GO) (Kyoto Encyclopedia of genes and genomes KEGG) p Masood s(hsa-mir-34b* hsamir-34b hsa-mir-34c-5p hsa-mir-449a hsa-mir- 39

4 449b*) [29] RNA : [1] Krausz C. Male infertility: pathogenesis and clinical diagnosis. Best Pract Res Clin Endocrinal Metab (2): [2] Okada H Tajima A Shichiri K et al. Genome-wide expression of azoospermia testes demonstrates a specific profile and implicates ART3 in genetic susceptibility. PLoS Genet (2):e26. [3] He Z Kokkinaki M Pant D et al. Small RNA molecules in the regulation of spermatogenesis. Reproduction (6): [4] Li J Liu Y Dong D et al. Evolution of an X-linked primatespecific micro RNA cluster. Mol Biol Evol (3): [5] Hayashi K Chuva de Sousa Lopes SM Kaneda M et al. MicroRNA biogenesis is required for mouse primordial germ cell development and spermatogenesis. PLoS One (3):e1738. [6] Zhong X Li N Liang S et al. Identification of micrornas regulating reprogramming factor LIN28 in embryonic stem cells and cancer cells. J Biol Chem (53): [7] Yi R Qin Y Macara IG et al. Exportin-5 mediates the nuclear export of pre-micrornas and short hairpin RNAs. Genes Dev (24): [8] Lund E Guttinger S Calado A et al. Nuclear export of microrna precursors. Science (5654):95-8. [9] Hutvágner G McLachlan J Pasquinelli AE et al. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science (5531): [10] Carmell MA Hannon GJ. RNase III enzymes and the initiation of gene silencing. Nat Struct Mol Biol (3): [11] Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol (5): [12] Lee RC Feinbaum RL Ambros V et al. The C.elegans heterochronicgene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell (5): [13] Lewis BP Burge CB Bartel DP. Conserved seed pairing often flanked by adenosines indicates that thousands of human genes are microrna targets. Cell (1): [14] Yang J Medvedev S Reddi PP et al. The DNA/RNA-binding protein MSY2 marks specific transcripts for cytoplasmic storage in mouse male germ cells. Proc Natl Acad Sci U S A (5): [15] Papaioannou MD Pitetti JL Ro S et al. Sertoli cell Dicer is essential for spermatogenesis in mice. Dev Biol (1): Ro S Park C Sanders KM et al. Cloning and expression profiling of testis-expressed micrornas. Dev Biol (2): [17] Yu Z Raabe T Hecht NB. MicroRNA Mirn122a reduces expression of the posttranscriptionally regulated germ cell 40

5 transition protein 2 (Tnp2) messenger RNA () by cleavage. Biol Reprod (3): [18] Yang Q Hua J Wang L et al. MicroRNA and pirna profiles in normal human testis detected by next generation sequencing. PLoS One (6):e [19] Hu LWua CL Guo CC et al. Identification of micrornas predominately derived from testis and epididymis in human seminal plasma. Clin Biochem (10-11): [20] Lian J Zhang X Tian H et al. Altered microrna expression in patients with non-obstructive azoospermia. Repord Biol Endocrinol (13):1-10. [21] Maatouk DM Loveland KL McManus MT et al. Dicer1 is required for differentiation of the mouse male germline. Biol Reprod (4): [22] Yan N Lu Y Sun H et al. A microarray for microrna profiling in mouse testis tissues. Reproduction (1):73-9. [23] Luo LYe L Liu G et al. Microarray-based approach identifies differentially expressed micrornas in porcine sexually immature and mature testes. PLoS One (8):e [24] Guo X Wu Y Hartley RS. MicroRNA-125a represses cell growth by targeting HuR in breast cancer. RNA Biol (5): [25] Wu J Zhu H Song W et al. Identification of conservative micrornas in saanen dairy goat testis through deep sequencing. Reprod Domest Anim (1): [26] Song R Ro S Michaels JD et al. Many X-linked micrornas escape meiotic sex chromosome inactivation. Nat Genet (4): Masood AH Christina B Petra L et al. MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns. Fertil Steril (1): [28] Bartel DP. MicroRNAs: genomics biogenesis mechanism and function. Cell (2): [29]Manikkam M Guerrero-Bosagna C Tracey R et al. Transgenerational actions of environmental compounds on reproductive disease and identification of epigenetic biomarkers of ancestral exposures. PLoS One (2): e ( ) Role of microrna in spermatogenesis Nan ZHANG Qin-ling YANG Ying-pu SUN (Reproductive Medicine Center the First Affiliated Hospital of Zhengzhou University Zhenzhou ) ABSTRACT MicroRNA() is a class of single-stranded RNA consisting of nucleotides and is one of the important members of non-coding RNAs. More and more evidence suggests that is closely associated with spermgenesis. In recent years by analyzing changes in different animal species the role of s that participant in protein synthesis process of spermatogenesis formation of lacuna indirect regulation of sperm motility maintaining spermtid and Sertoli cells maturation was found. With the development of sequencing and microarray technology to detect more and more spermatogenesis related s thus the sperm or testicular tissue of normal men successfully vasectomized men and different pathological types of non-obstructive azoospermia patients were also been detected. Key words: microrna (); male infertility; regulation of gene expression; meiosis; spermatogenesis 41