Structure and f unction of the seminal vesicle in male Bostrichthys sinensis 3

Transcription

1 50 (2) : , 04 A cta Zoologica S inica , , ,,,,,,,, Leydig,,,, PAS,, 17 PGE 2 PGF 2,, [ 50 (2) : , 04 ] Structure and f unction of the seminal vesicle in male Bostrichthys sinensis 3 ZHAN G Qi2Yong 133, HON G Wan2Shu 1, CHEN Shi2Xi 1, MA Xi2Lan 1, N I Zi2Mian 2 1. Department of Oceanography, Xiamen University, Xiamen , Fujian, China 2. Electron Microscope Laboratory, Xiamen University, Xiamen , Fujian, China Abstract The morphological structure and function of the seminal vesicles in male Bost richthys sinensis were studied by means of histology, transmission electron microscope and Enzyme2linked Immunosorbent assay. The results showed that the seminal vesicles, enclosed by capsula, were a pair of accessory glands to the testes. The seminal vesicle was divided in2 to many locule lumina by the connective tissue partition. Both capsula and partition consisted of smooth muscle fibers, blood capillaries, fibroblasts, fibrocytes and interstitial cells. Single epithelial cells, which were columnar, cuboidal or squamous in shape, were arranged along the partition. During the spawning season, rough endoplasmic reticulum, tubular cristae mitochondria and Golgi complex were well developed ; many secretory granules without membranes accumulated to the apical portion of the epithelial cell. The cell organelles degenerated and large vacuoles appeared after the secretory granules were released. The sphincter muscles were not found from the outlet of the seminal vesicles to the sperm ducts. Fibroblasts were characterized by synthesizing and secreting collagen. The morphology of interstitial cells in the seminal vesicle was similar to that of Leydig cells in the testes. At the peak period of spawning, a large number of spermatozoa, which were mixed with the secretion, appeared in proximal, middle and distal parts of the seminal vesicles. The epithelial cells inside the different locules of the seminal vesicle developed asynchronously. The results showed that sperm added to the seminal vesicle fluid could increase sperm motility, prolong sperm longevity, and improve the fertilization rates of sper2 matozoa. Fiber2like secretion of the seminal vesicles showed PAS positive reaction in dark purple2red colour, suggesting that the secretion should be mucopolysaccharide2mucin. The seminal vesicle fluid contained 17 OH2P, PGE 2 and PGF 2 which could induce female broodfish to spawn, indicating this fluid functions as a sex pheromone [ Acta Zoologica Sinica 50 (2) : , 04 ]. Key words Chinese black sleeper, Bost richthys sinensis, Seminal vesicle, Morphological structure, Ultrastructure, Function , (No No ) of China (No and No ) ] 33 (Corresponding author). ν 04 Acta Zoologica Sinica [ This research was funded by grants from National Natural Science Foundation

2 ( Claridae) ( Gobiidae) (Blennidae) (Bagri2 2 dae) ( Tripterygiidae) (Ba2 trachoididae) 211, ( Seminal vesicle) ( Sun2, dararaj, 1958 ; Rastogi, 1969 ; Fishelson, 1991 ; Fishelson et al., 1994 ; Lahnsteiner et al., 1992 ; Scaggiante et al., 1999),,,, ( Bost richthys 5-12 mm, 4-7 mm, sinensis) 4-13 mm, mm ( 1 ; : 1),,, cm, g,,, 43 Bouin,, 6 m, HE PAS Olympus DX % 1,,, J EM2100CX Cayman,, 1 A. B. P : D : SD : BC : Fig11 The seminal veicles of the male Bostrichthys sinen2 sis A. Right lobe. B. Left lobe. P : Proximal part. D : Distal part. SD : Sperm duct. BC : Blood capillary. PGE 2, PGF 2 -, ( 17 2, Diag2 : 2) nostic Systems Laboratories B IO2RAD550 ( n, (: = 6 ), 3) 14-29, 17 OH2P PGE 2 PGF 2, m, m t, P < 0105,, P < 10-17, m, m (: 12, 13), ,, Olympus 2216 m, m,,, m, m m, 95 % m,, 4, m, ( )

3 2 : 271, m, m,, m,, m (: 4, 5, 6),, (: 10), 112 m, 4-8,, (: 14, 15),,,,, (: 16) ( m),, m (: 17),,,,,, ( GSI),, ( SVSI),,,,, (, : 18),,,,,,,,,, 212, (: 5),, PAS (: 8),,,, , m, 1 (: 8),,,,, (:, 9) (: 19, ),,,,,, m,,,, (:, ),,, m, m m,,,, (, : 11),, m m, m, ,, m,,, (: 22),,

4 ,,, 2512, , (6417 %) ; : (1),, (56 %), 95 %,, 2 h 6 min ;,, 90 %, 1 h 47 min (2), 17 2 (17 OH2P), 85 %, PGE 2 PGF 2, 1 h 44 min, 17 OH2P ( ng/ ( 1), ml) ( ng/ ml ), ( P > 0105) ( 2) PGE 2 ( pg/ ml ) PGF 2 HCG L HRH2A 3 ( pg/ ml) 1 Table 1 Sperm motility and sperm longevity of Bostrichthys sinensis Habitat ( %) Sperm motility ( %) Sperm (h : min) Sperm longevity (h : min) + Sperm + seminal vesicle fluid ( %) 3 Sperm motility ( %) (h : min) 3 3 Sperm longevity (h : min) T ( ) S ( %) Wild Cultured % 3 The sperm motility means the percentage of swimming spermatozoa in total number of spermatozoa1 33 The sperm longevity means the duration from the moment of activation to the death of 95 % activated spermatozoa. 2 Table 2 Swimming speed of sperm varies in Bostrichthys sinensis + (h : min) Sperm Sperm + seminal vesicle fluid Habitat Duration after activation (h : min) ( m/ s) ( m/ s) T ( ) S ( %) Swimming speed ( m/ s) Swimming speed ( m/ s) Wild Cultured

5 2 : 273 PGE 2 ( pg/ ml ) PGF 2 ( pg/ ml) ( P < 0105), 17 OH2, Table 3 17 OH2P, PGE 2 and PGF 2 levels of seminal vesicle, testis and ovarian extract in mature Bostrichthys sinensis 17 OH2P Extract (ng/ ml) PGE 2 (pg/ ml) PGF 2 (pg/ ml) Seminal vesicle 6100 Testis Ovary ,,, P PGE 2 PGF 2, Fishelson et al. (1994) 3 ( Clarias gariepinus) 17 OH2P PGE 2 PGF 2, Leydig, Leydig, ( Clarias gariepinus) Leydig, 3 2HSD 3 2HSD (UDPGD),,,, ( Resink et al., 1987,, 1989 ; Schoonen et al., 1986, 1987, 1988 ; Hurk Sundararaj (1958) ( Het2 and Resink, 1992) eropneustes f ossilis),, 17 OH2 Nawar P PGE 2 PGF 2 Ξ, (1959) ( Clarias lazera),,,,, Rastogi (1969) PGE 2 ( M yst us tengara), Fichel2 ( References) son et al. (1994) ( Clarias gariepi2 Fishelson L, Comparative cytology and morphology of seminal nus), vesicles in male gobiid fishes. Japan. J. Ichthyol. 38 (1) : Fishelson L, Vuren J HJ V, Tyran A, Ontogenesis and ultrastruc2, ture of seminal vesicles of the catfish Clarias gariepinus. J. Mor2 phol. 219 : , Hurk RVD, Resink J W, Male reproductive system as sex pheromone producer in teleost fish. J. Exp. Zool. 261 : , ( Seminal vesicle), Lahnsteiner F, Seiwald M, Patzner RA, Ferrero EA, The semi2 nal vesicles of the male grass goby, Zosterisessor ophiocephal us ( Teleostei, Gobiidae). Zoomorphology 111 : Lahnsteiner et al. (1992) Nawar G, Observations on the seminal vesicle of the nile catfish Clarias lazera. Ann. and Mag. Nat. Hist. 11 : ( Zosterisessor ophiocephal us) Rastogi RK, Seminal vesicles and sperm duct of an Indian catfish Myst us tengara (, Ham. ), with particular reference to their season2 al cycle. Acta Anat. 72 : , Resink J W, Hurk RVD, Zoelen RFO GV, Huisman EA, The Ξ,,, 04.. ( ). Hong, WS, Zhao WH, Ma XL, 04. A preliminary study on the induction of spawning by sex pheromones in Bost richthys sinensis. Journal of Fsheries of China ( In press).

6 seminal vesicle as source of sex attracting substances in the African catfish Clarias gariepinus. Aquaculture 63 : Resink, J W, Schoonen WGEJ, Albers PCH, File DM, Notenboom CD, Hurk RVD, Oordt PGWJ, The chemical nature of sex attracting pheromones from the seminal vesicle of the African cat2 fish Clarias gariepinus. Aquaculture 83 : Scaggiante M, Mazzoldi C, Petersen CW, Rasotto MB, Sperm competition and mode of fertilization in the grass goby Zosterisessor ophiocephalus ( Teleostei, Gobiidae). J. Exp. Zool. 283 : Schoonen, WGEJ, Lambert J GD, Steroid metabolism in the seminal vesicles of the African catfish Clarias gariepi nus. (Burchell), during the spawning season, under natural conditions, and kept in ponds. Gen. Comp. Endrocrinol. 61 : Schoonen, WGEJ, Lambert J GD, Gas chromatographic mass spectrometric analysis of steroids and steroid glucuronides in the seminal vesicle fluid of the African catfish Clarias gariepi nus. Gen. Comp. Endrocrinol. 68 : Schoonen WGEJ, Lambert J GD, Oordt PGWJ V, 1988 Quantitative analysis of steroids and steroid glucuronides in the seminal vesicle fluid of feral spawning and feral and cultivated nonspawning African catfish Clarias gariepi nus. Gen. Comp. Endrocrinol. 70 : Sundararaj BI, The seminal vesicles and their seasonal changes in the Indian catfish Heteropneustes. Copeia (4) :

7 2 : (SV) ( T) (U) (UP) 2., ( ) ( ξ ) (CC) ( ) ( ), (CC) (CP) (LL) ( ξ ) ( ), ( ξ ) ( ) ( ), (LL) PAS, (LL) ( ) (S) ( ) ( ξ ) , ( ) ( ξ ) ( Ρ ) (BC) ( T) (SV) ( ) , (LL) (CC) (CP) (S) , , (RER) (M) ( G) (CM) , (LL) ( G) (SG), (CF) (LL) (S) (C) (LL) (LV), (MB) , ( ) ( ξ ) (N) (CF) ( ) , (N) BC : C : CC : CF : CM : CP : G: LL : LV : M : MB : N : RER : S : SD : G: SV : T : U : UP : Explanation of Plates Plate 1. The seminal vesicles (SV), testis ( T), urinary bladder (U) and urogenital papilla (UP) in male Bost richthys sinensis. 2. Transverse section through the seminal vesicle of male Bost richthys si nensis, showing the connective tissue capsula ( ) and partition ( ξ ) Smooth muscle fibers ( ) in the connective tissue capsula (CC) Columnar epithelial cells ( ) in the seminal vesicle, showing the connective tissue capsula (CC), the connective tissue partition (CP), locule lumi2 na (LL) and fibroblast ( ξ ) Cuboidal epithelial cells ( ) in the seminal vesicle, showing the bubble2like secretion ( ξ ) Squamous epithelial cells ( ) Necrotic cells ( ) became detached from the epithelium and accumulated in the locule lumina (LL) The secretion of seminal vesicle showed PAS positive reaction in dark purple2red colour, showing the locule lumina (LL) were filled with the sper2 matozoa ( ) and the secretion (S) The spermatozoa ( ) were enclosed by fiber2like seminal vesicle fluid ( ξ ) The sperm duct, showing epithelial cells ( ), spermatozoa ( ξ ), fibroblasts ( ), blood capillary (BC), testes ( T) and seminal vesicle (SV). Ρ The interstitial cells ( ) in the connective tissue partition of seminal vesicle

8 Plate 12. Longitudinal section through the seminal vesicle of male Bost richthys sinensis, showing the locule lumina (LL), connective tissue capsula ( CC), connective tissue partition (CP) and the secretion (S) Longitudinal section through the seminal vesicle of male Bost richthys si nensis, as showed above Epithelial cell in the seminal vesicle, showing rough endoplasmic reticulum ( RER), tubular cristae mitochondria (M), Golgi complex ( G) and cell membrane (CM) Epithelial cell in seminal vesicle, showing the locule lumina (LL) and Golgi complex ( G) Secretory granules (SG) accumulated to the apical portion of epithelial cell in the seminal vesicle, showing collagenous fiber (CF) in the locule lumi2 na (LL) The secretion (S) and cytoplasm (C) were released to the locule lumina (LL) Large vacuoles appeared in the epithelial cell of seminal vesicle, showing myelin body (MB) Fibroblast in the connective tissue capsula and partition, showing collagen fibrils ( ), collagenous fibers ( ξ ) and nucleus (N) Collagenous fibers (CF) in the locule lumina Fibrocytes ( ) in the connective tissue capsula and partition Interstitial cell in the connective tissue partition of seminal vesicle, showing its nucleus (N) BC : Blood capillary. C : Cytoplasm. CC : Connective tissue capsula. CF : Collagenous fiber. CM : Cell membrane. CP : Connective tissue partition. G: Golgi complex. LL : Locule lumina. LV : Large vacuole. M : Mitochondria. MB : Myelin body. N : Nucleus. RER : Rough endoplasmic reticulum. S : Secretion. SD : Sperm duct. SG: Secretory granule. SV : Seminal vesicle. T : Testis. U : Urinary bladder. UP : Urogenital papilla.

9 : ZHAN G Qi2Yong et al. : Structure and function of the seminal vesicle in male Bost richthys sinensis Plate ( Explanation at the end of the text)

10 : ZHAN G Qi2Yong et al. : Structure and function of the seminal vesicle in male Bost richthys sinensis Plate ( Explanation at the end of the text)