Index. endocytosis, 92 fat cells, 99 myelofibrosis, 390 nerves, 99, 100 sinuses, 90 Bone marrow fatty involution, red and yellow marrow,

Transcription

1 A Adherent layer cell types, 256 adipocytes, 261 endothelial cells, function, 262, 263 interactions, 261, 262 macrophage and epitheloid cell, Association of hematopoiesis and bone formation,209,210 B Bone role of in modulation of hemopoiesis, Bone marrow, 88, adventitial reticular cells, blood circulation, 88, 89 cell release, disorders, 389 endothelial cells, coated pits, endocytosis, 92 fat cells, 99 myelofibrosis, 390 nerves, 99, 100 sinuses, 90 Bone marrow fatty involution, red and yellow marrow, distribution normal and abnormal, 159, 160 Neuman's Law, 159 Temperature gradient hypothesis, 160 Bone marrow adipocyte, characteristics in longterm bone marrow culture, 180, 181 development, 170, 171 distinction from extramedullaryadipocyte, functional differences, function, heterogeneity,

2 446 structure, 171, 172 Capacity of cloned stromal cell lineages to support hematopoiesis in vivo, 214,215 Clorrlgenic hematopoietic stem cells developing on CEM enriched with stromal cells, , 211 Collagen, 369 measuremen~371 role, 369 structure, 370 type~ I, j73-378, 385, 390 II, 378, 379, 385 III, 379, 380, 385,390 rv,380,381,386,390 V, 381, 382, 388,390 VI, 382, 383 VII, 383 VIII, 383 IX, 384 X, 384 XI, 384 Collagenous matrix, 388 origin, 394 synthesis, 388 Components of bone marrow stroma cellular, fibroblastic reticular cells in vitro, fibroblastic reticular cells in vitro blanket cell, 227 fat cells, 226 fibroblasts, 225 macrophages, central macrophage, erythroblastic island, 231 macrophages dispersed in hematopoietic cord, 243, 244 perisinal macrophage, 243 other cellular components, 246, 247 stromal cell recognized by lanthanum uptake, 246, 247 reticular cells in vivo, advential reticular cells, fibroblastic reticular cells, 224, 225 intercellular matrix, 224 monoclonal antibody ER-HR 1,224

3 D Dexter, 272 Dexter stem cells, 254 E Ectopic implantation of bone marrow, Effect of membrane composi tion and pore size on hematopoiesis developing on stromal cell enriched CEM, cellulose acetate (Celotate ),199 cellulose ester (nitrates and acetates) membranes, 198 marrow stromal cells, polycarbonate (Nuclepore ),199 polyethylene linear, 199 polytetrafluoroethylene (Teflon or Mitex ), 199 polyvinyl chloride membrane (Polyvic ), 199 Egress from marrow 447 anatomy of leukocyte, 121, 122 anatomy of pathological states, 130, 131 anatomy of platelet, anatomy of reticulocyte, cell releasing factors, Electron microscopy of hematopoietic stromal cells developing on enriched CEM, Extracellular matrix hemopoietic, matrix molecules, bone Gla protein, 408 bone morphogenetic protein (BMP), 410 collagen, fibronectin, 403,404 laminin, 404, 405 osteocalcin, 408 ostrogenesis, 420 osteogenin, 419 proteoglycans, 405,409 regulation of cell growth and differentiation, 401, 402

4 448 G Growth factor production, H Hematopoiesis on CEM implanted in Sl/Sld mice, 193, 194 Hematopoiesis on ip implanted CEM enriched or coated with hematopoietic stromal cells, bone stromal cells, 195 marrow stromal cells, 195 regulation of stromal cells, splenic stromal cells, 196 stromal cells from Dexter layers (LTMC), 212 stromal cells from regenerating endosteum, 195 Hematopoiesis on uncoated ip implanted CEM, endothelial cells, 190 fibroblasts, 190 macrophages-monocytes, 190 types of stromal cells identified on fait CEM, 194 Hemopoiesis abnormal, aplastic anemia, comparison with immunodeficiency, 354, 355 immunosuppressive therapy, in vitro suppressor mechanisms cell mediated, humoral, pathogenesis of marrow aplasia, 361 role of NK cells, 362 Hemopoiesis control of, stochastic model, normal, role of T -lymphocytes BPA production of, 336, 337 in growth of BFU-E,

5 interferon (a and y) mediation of T-cell effects, production of CSF, 350,351 ricin-antibody depletion of, role in granulopoiesis,350 T-cell markers, role of monocytes, roles of monocytes BPA,352 in erythroid islets, 352 MRA,351 production of IL-l, tumor necrosis factor, intereron, 352,353 prostaglandins, 351 role of NK cells, 353, 354 graft rejection, 353 LAK cells, 353, 354 NK cell-derived colony inhibiting 449 activity (NA CIA), 353 regulation of, cellular interactions, Hemopoietic cells biophysical properties, 104 cell chemotaxis, 106 cell deformability, 105, 106 cell motility, 104, 105 cell surface features, 107 Hemopoietic inductive microenvironment (HIM), evidence, bone marrow colonies, 29,30 "cure" of Sl/Sld, marrow stroma transplantation, 32, 33 rat CFU-S in mouse microenvironment, tetanus toxoid sensitization, 38, 39 whole spleen transplantation, 30-32

6 450 cellulose acetate membranes,42 CFU-S heterogeneity, hemopoietic hormone receptors, 50, 51 hypothesis, mast cell differentiation, 39,40 roles of marrow, spleen, thymus stroma, 40, 41 Hemopoietic microenvironment, nature of stromal cells, definition, 292 primary stromal cell cultures, Dexter cultures, 293 other systems, putative functions, hemopoietic inductive microenvironment (IDM),288 stochastic model of stem cell self-renewal, Hemopoietic microenvironments concept of, 1-4 homing, 5-9 effect of erythropoi- etin deprivation, lineage decision, primary spleen colonies types of, 9-18 secondary spleen colonies, radiation protection, 4 spleen colony assay definition,s Hemopoietic microenvironments historical perspectives, 1-78 Hemopoietic stromal cell lines, adipogenesis, 306, 307 cell surface markers, 309 classification, endothelial-adipose cell, 309, 310 endothelial-like cell, 310 fibroblast of advential reticulum cell, 310 fibroendothelial cell, 311 long-term maintenance of hemopoiesis, macrophage, 311

7 CSF, other soluble regulators of hemopoiesis, 316,317 cytoplasmic enzymes, 307 derivation and growth, extracellular matrix, 308, 309 interactions between components of stroma, 324,325 leukemia cell inhibitory activity (LCIA), list of human and murine stromal cell lines, morphology, promotion of leukemia cell growth, transplantation, tumorigenesis, 323, 324 Hodgkin's disease, L Long-term bone marrow cultures, 55-59,434 matrix proteins, regulation by matrix 451 components, 416, 417 Lymphoid inductive microenvironments, Lymphoid long-term culture, M Matrix proteins secreted by hematopoietic stromal cells, bone matrix powder (demineralized), collagen I, 203 collagen II, 203 collagen IV, 203 glycosaminoglycans,201 proteoglycans, 201 tooth matix powder (demineralized), Microenvironment bone marrow, cellular components, fibroblasts, functional abnormalities, 435, 436 macrophages,

8 452 T-Iymphocytes, clinical implications, 434,435 aplastic anemia, 434, 435 Microenvironments in malignancy, 77, 78 Microscopic methods in vivo, basic methods, 142, 143 perfused preparations, 142, 143 surgically-exposed organs in situ, 142 visualization through windows contained within chambers implanted in ectopic sites, 143 ~pi-illumination methods, 146 methods to study hemopoietic tissue, erythropoietin as a vasoactive mediator, other vasoactive substances, 150, 151 role of prostaglandins, adenosine, 153 sources of vasoactive substances, 150, 151 transillumination methods, compound trinocular microscope, 144, 145 quartz, glass, plastic light rods, fiber optic light guides, 143, 144 use of specific wavelengths of monochromatic light, 145, 146 Microvasculature within hemopoietic tissue, 141 Model of stromal regulation, Myelofibrosis, 390 o Origin of hematopietic stromal cells (donor or host), 207, 208

9 p Proliferation hemopoietic stem cell, 433,434 inhibitors and stimulators humoral, 435 regulation by the marrow microenvironment433,434 R Stromal cells, of erythroid colonies, of granuloid colonies, 64, 65 Studies in different species, T TC-1 cell line, w 453 Radiation sensitivity of hematopoietic stromal cells, 207, 208 Whitlock-Witte, 273 Wound healing, 392 s Serum supplement, 254 Stem cells circulation, 131, 132 reentry into marrow, 131, 132 Stromal cell lines from other times, 325, 326