Quiz #1. BIO200 January 11, point each
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1 Quiz #1 January 11, The primary amine group of an amino acid has a pka of 10 and the carboxylic acid group of an amino acid has a pka of 2. The side chain of the amino acid alanine is a methyl group ( CH3). Draw the structure of alanine as it would appear at ph 12, including all formal charges. 2. The structure of glycerol is shown below. As part of synthesizing a triglyceride, attach one C8:1 fatty acid to the glycerol. 3. Transmembrane domains are usually made of what secondary structure? Why does this particular secondary structure work well as a transmembrane domain? 4. The reaction of 3-phosphoglycerate (3-PG) to 2-phosphoglycerate (2-PG) is endergonic ( G o = +1.0 kcal/mol). If this reaction reaches equilibrium, what can we conclude about the ratio of 3-PG to 2-PG? (your answer can be qualitative no numbers required) 5. Starch and cellulose are both polymers of what molecule?
2 Quiz #2 January 18, The structure of a monosaccharide is shown to the right. Circle every term that correctly describes it. Aldose Chellose Hexose Ketose Pentose Tetrose 2. Among the following molecules, circle the one carbon atom that is the most reduced. 3. The molecule on the right and ADP are the products of what enzyme? 4. The Transition Step is catalyzed by Pyruvate Dehyrogenase. Name the product molecule that contains two carbon atoms (standard abbreviations are acceptable). P 5. Name the biochemical pathway that effectively reverses glycolysis.
3 Quiz #3 February 1, Name the linker histone: 2. Even though Primase is needed for replication in a living cell, we don t need to add it for PCR. Why is Primase not needed for PCR? 3. Telomerase is an unusual form of DNA polymerase because it A. uses a RNA template. B. adds to a 5 end. C. does not require a template. D. works in reverse from most DNA polymerases. E. is not found in the nucleus. 4. Gel electrophoresis can be used to measure the and the _ of a DNA molecule. 5. Name the structure shown in this key figure from the text:
4 Quiz #4 February 8, In what structure do we find a sugar-phosphate bond between a 5 and a 2 carbon? 2. I want to know in which of ten different cell types the YFG gene is actively expressed. What technique should I use? 3. Which factor is needed for the elongation phase of translation? A. TFIIB B. eif4a C. RF D. EF-Tu E. IF1 4. What amino acid should be attached to a trna with an anticodon of 5 -UAC-3? 5. List all codons that can be recognized by a trna with an anticodon of 5 -UAC-3. Wobble Rules Anticodon Base Codon Base C G A U U A or G G C or U I U, C or A
5 Quiz #5 February 22, 2013 For each of the following, name the protein that gets polymerized: A. This protein is the most abundant protein in the ECM found in humans. B. This is one protein that can polymerize to form an intermediate filament. C. The polymerization of this heterodimer forms the 9+2 structure in a flagellum. D. This protein polymerizes to form the thin filament of animal skeletal muscles. E. Name the protein whose polymerization creates the basketlike cages shown on the right.
6 Quiz #6 March 1, Name the one cell type where each of the following activities happens. (½ point each) A. cell Glycogen is depolymerized to release glucose. Insulin is secreted from this cell type. Carbon atoms in glucose are stored as lipids. Low blood glucose concentrations are sensed by this cell type. B. cell C. Adipocyte D. Fibroblast E. Hepatocyte F. Neuron 2. In the four boxes below the shown action potential, indicate if the voltage-gated Na+ channel is in it s Closed (C), Inactivated (I) or Open (O) conformation by writing the corresponding letter in the box. (2 points) 3. Name the molecule produced from ATP by adenylyl cyclase, as shown in Figure on the right. (1 point)
7 Quiz #7 March 8, A heterotrimeric G protein is active when A. the G subunit is bound by the hormone. B. the G subunit is bound to G /G and bound to GDP. C. the G subunit is bound to G /G and bound to GTP D. the G subunit is alone and bound to GDP. E. the G subunit is alone and bound to GTP. 2. The presence of a kinase cascade in a signal transduction pathway allows for A. signal amplification. B. long-term potentiation. C. negative feedback. D. crosstalk. E. paracrine signaling. 3. Caspases become activated during A. apoptosis. B. G 0 exit. C. mitosis. D. necrosis. E. EGF signaling. 4. camp directly activates which protein? A. PKA B. PLC C. CDK D. GNEF E. Glycogen synthase 5. Which one statement is true about kinetochore microtubules in M phase? A. They interact with other kinetochore microtubules. B. Their ends are attached to two centromeres. C. Their + ends are attached to kinetochores. D. They get longer during Anaphase. E. They are degraded by the Anaphase Promoting Complex (APC).
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