PINK PEPPERSLIM Spicy Fats Burner
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1 PINK PEPPERSLIM Spicy Fats Burner
2 PINK PEPPERSLIM Spicy Fats Burner Pink Pepperslim is a slimming oil derived from Pink Pepper with powerful lipolytic properties. A concentrated oil from pink berries, Pink PepperSlim sets itself apart from traditional fat burners due to its pre-lipolytic action. Its pre-lipolytic activity focuses on large lipid droplets. This slimming agent reduces their protection to make them more available to lipases. It also stops them forming instead of small lipid droplets which are easier to burn. Prepared in this way, its lipolytic action is multiplied and it generates significant centimetre loss in both men and women. Finally, the slimming effect of Pink PepperSlim is rounded off by stimulating micro-circulation. BE INSPIRED Pink PepperSlim is a pure oil extracted from Schinus terebinthifolius seeds using the supercritical CO2 technique. The false pepper plant Schinus terebinthifolius is a specie of tree whose berries provide a spice known as pepper rosé, pink berries or Aroeira. It originated in Brazil and is nowadays cultivated in the island Réunion (next to Madagascar) for its berries known as the Pink Gold of Réunion. The tree can measure up to 15 metres high. Its leaves are evergreen, aromatic, creased and can reach 15 cm in length. They give off a peppery odour or an odour of terebinth. The off-white flowers hang in bunches. From these, a discretely perfumed honey is produced. The pink berries, aromatic fruits, coloured coral red in summer, are 8 mm in diameter and have a peppery smell. The tree secretes an aromatic resin which is also called incense. Pink pepper has a fairly sweet taste, almost sugary at first, then aromatic. They are often sold preserved in brine or used as dried berries. They are eaten whole or crumbled, i.e. carefully crushed between the fingers.
3 OBSERVE MEMO OF CUTANEOUS BIOLOGY The different stages of fat storage Adipophilin Perilipin A 1 - Pre-adipocyte differentiation Differentiation of pre-adipocytes, which up till that point are not capable of storing fat, in the young adipocyte is the first step in the fat storage process. Fats are taken on by a protein called adipophilin and stored as small lipid droplets. Level of expression Process of Differentiation 2 - Maturing of the young adipocyte Development of young adipocytes into mature adipocytes is the second fat storage step. 1 2 The second step is accompanied by a fall in the level of adipophilin and an increase in the level of Perilipin A. Pre adipocyte Fat storage: Young adipocyte Fat storage: + Mature adipocyte Fat storage: +++ Perilipin A takes on the fat molecules during the second step. Fats are stored in the form of a single large lipid droplet. In addition to take on fats, Perilipin A protects large lipid droplets. By distributing itself over the droplet surface, it creates a sort of cellular shield which restricts access by lipases to the fat reserves. Pink PepperSlim : two fold lipolytic action Adipophilin Perilipin A 1 - Pre-lipolytic action To burn fat, it is important that the lipases can access them easily. Also, it is easier for them to burn up small lipid droplets than large ones. Inhibiting Perilipin A synthesis acts on both these factors: - it reduces the protection on large lipid droplets making them easier to metabolise - it reduces regulation of fat molecules to form large lipid droplets and so promotes formation of small lipid droplets which are much easier to metabolise. 2 - Lipolytic action Level of expression Process of Differentiation 1 2 Since the fat reserves are now more accessible, lipolysis is more effective: - increased amount of glycerol released - increased fatty acid quantities released Pre adipocyte Fat storage: Young adipocyte Fat storage: + Mature adipocyte Fat storage: +++
4 IN-VITRO TESTS PRE-LIPOLYTIC ACTION Pink PepperSlim inhibits Perilipin A expression by 23% This inhibition leads to: 2 μm 2 μm reduced protection of large lipid droplets against lipolytic agents reduced ability to take on fats during development of small lipid droplets into large lipid droplets Pink PepperSlim increases adipophilin level by 6% This increase directly results from Perilipin A inhibition. Since small lipid droplet maturation is restricted, the number of small drops increases compared to large drops and adipophilin level increase accordingly. Pink PepperSlim stops the formation of large lipid droplets in favour of small ones 2 μm 2 μm The colouring of lipid droplets by the red oil shows the inhibitory effect of Pink PepperSlim on large lipid droplets formation and fat storage. Differentiated human pre-adipocyte cultures Analysis of Perilipin A and Adipophilin expression: RT-qPCR Perilipin A and Adipophilin visusalisation: Immunofluorescence Fat storage visualisation: red oil Control Pink PepperSlim,5% LIPOLYTIC ACTION Pink PepperSlim displays powerful lipolytic activity. From.25%: Release of glycerol: +24% Release of fatty acids: +1253% Fatty Acids (μm) Release of Fatty Acids Release of Glycerol Glycerol (μm) At.5% : Release of glycerol: +91%* Release of fatty acids: +1737%* *p<.5 Student Test Differentiated human pre-adipocyte cultures Fatty acid and glycerol amounts released measured by optical density 1 5 Control Pink PepperSlim,25% Pink PepperSlim,5% 4 2 Pink PepperSlim displays greater lipolytic effectiveness than reference molecules Theophylline:.18% Isoproterenol:.2% Caffeine:.19% Pink Pepperslim:.5% With its pre-lipolytic action, Pink PepperSlim sets up optimum lipolytic conditions: small lipid droplets are in the majority which are easier to metabolise than large ones and the protection on already formed large lipid droplets is weakened. Its lipolytic action is facilitated and more powerful than that of reference lipolytic molecules. Fatty Acids release (% of control) Control +1737% Release of Fatty Acids +158% +2% +81% Theophylline Isoproterenol Caffeine Pink PepperSlim
5 REVEAL CLINICAL TEST SLIMMING ACTION Protocol: - Application of a cream containing.1% Pink PepperSlim twice a day for 28 days - 18 female volunteers: reductions measured in centimetres on the waist, hips and thighs - 7 male volunteers: reductions measured in centimetres on the abdominal belt line RESULT: The use of Pink PepperSlim led to a loss in centimetre in men and women of up to 2 cm. Size reduction in women Size reduction in men Average loss Max. loss - 2 cm -,15 cm -,7 cm -,7 cm Thighs Hips Average loss Max. loss -,4 cm -,7 cm Belt line -,4 cm - 2,2 cm Waist -2,5-2, -1,5-1, -,5, Centimetre reduction (in cms) -,8 -,6 -,4 -,2, Centimetre reduction (in cms) EFFECT ON MICROCIRCULATION Protocol: - Single application of a cream containing.1% Pink PepperSlim on the forearm - 1 volunteers: variation in speed of blood circulation measured by the Doppler Effect and by resistance to blood circulation RESULT: Pink PepperSlim activates microcirculation by 16% after 1 hour without any vasodilation Resistance to blood flow is used to determine whether the product has effects on blood vessel diameter. Pink PepperSlim induces a slight increase in resistance after 3min, which then normalises after 6min. This weak variation demonstrate this absence of both vasoconstrictive and vasodilation effect *p<.5 Student Test % variation compared to T vs placebo ,8% T5 min Speed of circulation Resistance to circulation +13,9% T1 min +15,6%* T3 min +16,5%* T6 min Due to its pre-lipolytic action, Pink PepperSlim optimises its effects and metabolises up to 1% more fatty acids. This powerful lipolytic activity generates size reductions in men and women of up to 2 cm in 28 days. Land plant origin Liposoluble active ingredient in-vitro test in-vivo test Natural Ecocert validation
6 PINK PEPPERSLIM: Spicy Fats Burner Cosmetic Actions = Inhibits Perilipin A synthesis = Stops formation of large lipid droplets = Activates release of glycerol and fatty acids = Reduces the circumference of the waist, hips and thighs in women = Reduces the abdominal belt line in men = Stimulates microcirculation INCI Name Schinus terebinthifolius seed extract Recommended % of use PINK PEPPERSLIM :.1% Technical department : infotech@codif-recherche-et-nature.com Commercial department : commercial@codif-recherche-et-nature.com 7, rue du Commandant l Herminier - BP Saint-Malo cedex - FRANCE Tel : Fax : CODIF Recherche & Nature is committed to sustainable development. All our wastewater is processed and purified using filtrating gardens. A succession of airtight horizontal and vertical filters is used to depollute wastewater by the roots of plant species chosen for their draining properties. Iris, water mint, flowering rush, willows etc now form part of the image of the brand which is recognized for its commitment to the preservation of natural resources RPDOC14GB
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