Green innovation for slimming
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1 Green innovation for slimming Temporary version
2 An exclusive slimming active extracted from natural caffeine. Sveltam is a slimming ingredient which acts on adipocytes at two levels: block adipogenesis stimulate lipolysis Glucose ADIPOCYTE Glycerol camp Fatty Acids G3PDH Glycerol-3-P Lipid droplet Triglycerides Lipase Glycerol + Fatty Acids Sveltam is composed of an isomix of xanthine derivatives which is produced by an enzymatic transformation of natural caffeine, using an innovative biotechnology process. G3PDH: Glycerol 3-phosphate dehydrogenase
3 An exclusive slimming active extracted from natural caffeine. Sveltam is a slimming ingredient which acts on adipocytes at two levels: block adipogenesis stimulate lipolysis Glucose ADIPOCYTE Sveltam Glycerol camp Fatty Acids G3PDH Glycerol-3-P Lipid droplet Triglycerides Lipase Glycerol + Fatty Acids Sveltam is composed of an isomix of xanthine derivatives which is produced by an enzymatic transformation of natural caffeine, using an innovative biotechnology process. G3PDH: Glycerol 3-phosphate dehydrogenase Block adipogenesis Stimulate fat destocking
4 Anti-adipogenesis effect (in vitro tests) Protocol: Sveltam was tested for its ability to block adipogenesis. G3PDH* activity was measured on preadipocytes 3T3-L1 cultured in adipogenesis inducing medium and treated with different concentrations of the product. *G3PDH: Glycerol 3 phosphate dehydrogenase, enzyme involved in the accumulation of triglycerides G3PDH inhibition efficiency (%) Measurement of G3PDH activity * * % % * 33 % *p.1 compared to control, Student s t Test Result: Sveltam significantly inhibits G3PDH enzyme by 33% compared to control. Positive control: genistein (3µM) Sveltam.12% Sveltam.25%
5 Anti-adipogenesis / anti-stocking effect (in vitro tests) Protocol: Sveltam was tested for its ability to block adipogenesis. Preadipocytes 3T3-L1 were cultured in adipogenesis inducing medium and treated with different concentrations of the product. The lipid accumulation was evaluated with the Oil Red O staining method. Lipid accumulation inhibition (%) Inhibition of lipids accumulation * 7 % * 9 % * 15 % Result: Sveltam significantly decreases intracellular lipids accumulation by 15% compared to control. Positive control: genistein (3µM) Sveltam.12% Sveltam.25% *p.1 compared to control, Student s t Test
6 Stimulation of lipolysis (in vitro tests) Protocol: Sveltam was tested for its ability to stimulate lipolysis. The intracellular camp* was measured on fully differentiated adipocytes treated with different concentrations of the active. *camp: cyclic Adenosine Monophosphate camp (pmol/mg prot.) Intracellular camp production +64%* +122%* *p.1 compared to control, Student s t Test Result: Sveltam significantly enhances camp up to 122% compared to control. Control Sveltam.12% Sveltam.25%
7 Stimulation of lipolysis (in vitro tests) Protocol: Sveltam was tested for its ability to stimulate lipolysis. Therefore, glycerol and fatty acids production (the two products of the lipids stocks hydrolysis) were measured on differentiated adipocytes treated with different concentrations of the active. Result: Sveltam significantly increases glycerol and fatty acids production up to 282% and 167%, respectively compared to control. Glycerol (µg/mg prot.) Glycerol production +87%* +282%* Free fatty acids (nmol/mg prot.) *p.1 compared to control, Student s t Test Free fatty acids production Control Sveltam.12% Sveltam.25% %* +167%*
8 TECHNICAL INFORMATION INCI: Origin: Preservation: Appearance: Solubility: Dosage: Processing: Intellectual property: Regulatory status: Claims: Glycerin, Caffeine, Theobromine, Methyl Xanthine, Paraxanthine Camellia sinensis & organic synthesis Preservative free Faint yellow solution Water-soluble.5% - 5% Can be incorporated in any formula in liquid form at ph below 7. and temperature below 45 C Patented JP, KR*, HK, AU*, ASEAN, USA, CA, South-Am., Mid-East, EU Slimming (block adipogenesis, activate destocking) * Registration pending Ocular irritation: Skin irritation: Mutagenicity: Phototoxicity: Sensibilization: SAFETY ASSESSMENT Het cam assay - practically non irritant at 1% OECD 439 assay - non irritant at 1% Ames assay - OECD non mutagenic PTC assay - non phototoxic HRIPT assay - non sensitizing at 5%
9 Thank you Induchem AG Industriestrasse 8A CH-864 Volketswil Induchem USA, Inc. 535 Fifth Avenue, Floor 23 New-York, NY117 Induchem France SAS 171 Bis Avenue Charles de Gaulle FR-922 Neuilly Sur Seine
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