Calcium Dysregulation in Acute & Chronic Pancreatitis

Transcription

1 PancreasFest 217 Session IX: Mechanisms of Chronic Pancreatitis Calcium Dysregulation in Acute & Chronic Pancreatitis Guy Groblewski, PhD July 28, 217

2 Ca 2+ Signaling in Acinar Cells David Yule Pancreapedia 212

3 Ca 2+ Sensors in Acinar Cells EF Hand Motif Containing Proteins - Ca 2+ /Calmodulin dependent protein kinases II and III () - Ca 2+ /Calmodulin dependent protein phosphatase Calcineurin () - Cab45b C2 Domain Containing Proteins () Multiple Downstream Substrates - Protein Kinase C isoforms () - Synatoptagmin 1, 3, 6 and 7 - Synaptotagmin-like proteins - CALDAG-GEFI and III Others - Ca 2+ Activated Cl - channels ANO1 and ANO2 - Plasma membrane and SERCA pumps

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5 Tumor Protein Biochemistry coiled-coil Structural Features pi 4. pi 1. pi Peripheral membrane protein - Binds anionic phospholipids (PS, PtdIns Phosphates) - Undergoes Ca 2+ -dependent phosphorylation at serine Exists as a dimer - Copurifies with clathrin coated vesicles - Binds to annexin VI, MAL2, PLP2, ATM, ADRP and TIP47, Rab5 and ATG16L1 - Regulates constitutive and stimulated secretion in acini 5

6 /CRHSP-28 is Highly Expressed in Exocrine Glands kda 28 mucosa Salivary and Lacrimial Small Intestine Stomach Groblewski et. al AJP 1999

7 localizes to a Sub-apical trans-golgi and Endosomal Compartments Overlay Overlay Control CCK (1 pm, 2 min) MAL2 EEA1 TGN38 CCK (1 pm, 2 min) Overlay Messenger et al. AJP 213

8 localizes to sub-apical trans-golgi and endosomal compartments PNS Cyto ZG CM VAMP 2 VAMP 8 T EEA1 Thomas et al. AJP 24

9 The Constitutive-like and Minor Regulated Pathways Function in Parallel to the Classic Zymogen Granule Pathway Constitutive-Like Pathway (CLP) Minor Regulated Pathway (MRP) Classic Zymogen Granule Pathway Apical Membrane Recycling Endosome Mature Zymogen Granules Lysosome Late Endosome Early Endosome Immature Granule trans Golgi

10 Early to late endosome trafficking controls secretion and zymogen activation A Lys LE CLP RE Rab11a Rab5 PIKfyve EE ZG ZGP B Lys LE CLP RE EE ZG ZGP M6PR AP1 ISG M6PR AP1 ISG trans-golgi trans-golgi C CLP RE ZGP ZG Lys LE EE M6PR AP1 ISG trans-golgi Messenger et al. CMGH Nov. 215

11 PIKfyve Controls Intracellular Zymogen Activation Intracellular Trypsin Activity (Fold over Basal) Basal 1nM CCK YM21636 (μm) Intracellular Trypsin Activity (Fold over Basal) Basal 1nM CCK TLC Control YM21636 NNK HA-PIKfyve Human Acini Intracellular Trypsin Activity (Fold over Basal) Basal 1nM CCK GFP WT K1877E PIKfyve GFP Wt K1877E HA-PIKfyve Intracellular Trypsin Activity (Fold over Basal) Control YM21636 Akt VIII Basal 1μM CCh 1mM CCh Messenger et al. CMGH Nov. 215

12 The constitutive-like pathway is essential for VAMP8-mediated zymogen granule maturation and exocytosis Apical Membrane Recycling Endosome Lysosome Late Endosome Constitutive-like and Minor Regulated Pathways VAMP8 Early Endosome Classic Zymogen Granule Pathway VAMP2 Mature Zymogen Granule Immature Granule Amylase Secretion CCK 1 pm VAMP2 (ATP-independent) VAMP8 (ATP-dependent) Time (min) trans Golgi Messenger et al. J Biol Chem 215 Messenger S, Jones E. et al. J Biol Chem 217

13 VAMP8-/- Acini are Protected from High Dose-CCK Mediated Zymogen Activation Total Cellular Trypsinogen (RFU/ μg Protein) : 2.9 Total Cellular Trypsinogen (RFU/ μg DNA) : 4.5 Wt VAMP8-/- Intracellular Trypin Activity (Fold of Wt basal) WT VAMP8 -/- Basal Total cellular trypsin Max CCK SupraMax CCK Intracellular Trypin Activity (Fold of Wt basl/ μg DNA) WT VAMP8 -/- μg DNA Basal Max CCK SupraMax CCK LDH Release (% of Total) Wt VAMP8-/- Basal Max CCK SupraMax CCK

14 localizes to apical membrane, trans-golgi and endosomal compartments CLP ZGP RE Rab11a/ ZG Lys LE LAMP1/ EE Rab5/ ISG TGN38/ trans-golgi Thomas et al. AJP 24

15 regulates acinar cell secretion Permeabilized Acinar Cells Adenovral Expression of Amylase Release (% of total) Ionomycin Control (basal) Time (min) LAMP1 Surface Labeling in CHO cells S136/A S136/D Thomas et al. JBC 21 Thomas et al. AJP 21 Messenger et al. AJP 211

16 Endosomal regulatory proteins are rapidly lost during pancreatitis B Endosome C Proteosomal Inhibitor Cathepsin Inhibitor ERK ERK Expression (Fold of basal) Basal CCK-8 MG132 MG132 + CCK Basal CCK-8 Ca74Me Ca74Me + CCK-8 Messenger S, Jones E. et al. J Biol Chem 217

17 Enhanced CLP/MRP trafficking by or Rab5 overexpression blocks zymogen activation Messenger S, Jones E. et al. J Biol Chem 217

18 colocalizes with autophagic vacuoles containing LAMP2 and LC3-II following acinar stimulation Basal CCK (3 pm) CCK (1nM) /LAMP2 LC3-II All 3 Messenger S, Jones E. et al. J Biol Chem 217

19 Overview of the macroautophagy (autophagy) pathway Phagophore Autophagosome Autolysosome ATG16L LC3 Lysosome Amino acids, lipids, sugars, etc.

20 Atg16L1 is an autophagy regulatory protein involved in LC3 lipidation Phagophore Autophagosome LC3-II LC3-I

21 Secretory autophagy Marisa Ponpuak1,2, Michael A Mandell2, Tomonori Kimura2, Santosh Chauhan2, Ce dric Cleyrat3 and Vojo Deretic2 Dual roles for autophagy: Degradation and secretion of Alzheimer s disease Ab peptide Per Nilsson and Takaomi C. Saido Different members of the IL-1 family come out in different ways: DAMPs vs. cytokines? Sonia Carta, Rosa Lavieri and Anna Rubartelli Cell Biology Unit, IRCSS Azienda Ospedale Università San Martino-IST, Genoa, Italy

22 The CLP/MRP coordinate a cellular clearance pathway Amphisome Exocytosis LAMP1 LC3 Late Endosome LAMP1 Clathrin-coated Endocytosis Recycling Endosome X X X X X X X X X X X X ATG16L1 X X X X X Apical membrane LC3 -ATG16L1 Degradation Lysosome Autophagosome LC3 Phagophore Assembly

23 Acknowledgements Groblewski Lab Diana Thomas Scott Messenger Elaina Jones Michelle Cooley Conner Haulthus Arielle Laski Kali Deans Nathalie Ly Michelle Falkowski Yale University/Veterans Administration Fred S Gorelick UCLA/VA/Cedar Sinai - Stephen J Pandol - Anna Gukovsakya - Ilya Gukovsky - Olga Mareninova - Aurelia Lugea - Richard Waldron Funding NIH-NIDDK