SUBLIGANA. Feel sublime with a perfect skin. Anti-imperfections botanical active sourced in Madagascar by Serdex
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1 SUBLIGANA Feel sublime with a perfect skin Anti-imperfections botanical active sourced in Madagascar by Serdex
2 SUBLIGANA TM H H H SUBLIGANA is an active ingredient based on the sustainable use of the malagasy biodiversity to address blemished & acne-prone skin. H QUERCITRIN H 3 C H H H BENEFITS N VLUNTEERS Anti-imperfections care: -34% of whitehead at D28 vs placebo. TARGETED ACTIN Soothing effect: decrease of lipase activity (-39%), modulation of interleukins (IL-1: +157%; IL-12: -93%). Antioxidant: protective effect against lipid peroxidation (-79%). Dermopurifying effect: efficacy against Propionibacterium acnes. 1
3 H H H H H H H TAXIFLIN 3-ARABINSIDE TYPE STRUCTURE Characteristic by its yellow-orange downy young parts H ARNGANA, THE FREST GUARD F MADAGASCAR Plant Family Malagasy name rigin Botanical description rgan Phytochemistry Harungana madagascariensis Hypericacacee Harongana Madagascar Evergreen tree, with height up to 8m; characteristic by its yellow-orange downy young parts & by its small flowers with white petals. A gummy red-orange substance flows when its bark is cut. Leaves Taxifolines (Taxifoline-Arabinoside-1 & 2) & Quercitrin Habitat: Harongana is a plant from the center/east of Madagascar. By forming a physical & a repulsive barrier, this pioneer plant protects primary forest areas from attacks. It is also capable of restoring degraded landscapes by stabilizing soils & allowing ferns establishment: it is considered by botanists as a «forest guard». Traditional use: Leaf decoctions of Harungana madagascariensis are traditionally used against asthma & skin disorders, leaf juice for wound healing & bark decoctions against gastric ulcers. Good collecting practices: According to preliminary variability studies to ensure a repeatable & reliable phytochemical composition in quercitrin and taxifoline arabinosides, Harungana madagascariensis is collected wild from ctober to March in the high plateaus area in an Ecocert certified area. Sustainable development: Serdex is a UEBT member & contributes to the development of Madagascar through equitable sharing of the benefits on the living conditions of plant collectors & the development of local industrial partners. Serdex is a UEBT member: Good Collecting Practices & Sustainable Development 2
4 ACNE IS AFFECTING ABUT 8% F PERSNS AT SME PINT IN THEIR LIVES 1 Acne is a chronic inflammatory disease of the pilosebaceous unit induced by androgenic & non-androgenic factors. Acne can lead to different types of comedones, also called retentional lesions: whitehead (microcyst, closed comedo) & blackhead (open comedo). Comedones can lead to inflammatory lesions, such as papules pustules & nodules. In the long run, those lesions can induce scars & Post Inflammatory Hyperpigmentation (PIH). Four processes have a role in the formation of acne lesions: inflammatory mediators released into the skin, alteration of the follicular keratinization process, increased & altered sebum production & follicular colonization by Propionibacterium acnes. Acne-prone skin is related to a new theory that a primarily cutaneous & systemic oxidative stress is involved 2. Taking into account this theory, we have developed a mode of action focusing on soothing & anti-oxidant effect. 1: Questions and Answers About Acne. National Institute of Arthritis and Musculoskeletal and Skin Diseases, 21, jan. 26 [consulted in july 216]. Available on: / 2: Bowe et al.. Clinical implications of lipid peroxidation in acne vulgaris old wine in new bottles, Lipids in Health and Disease 21. 3
5 SUBLIGANA TM : BILGICAL APPRACH N ACNE-PRNE SKIN Acne: causes, factors & consequences on skin Effect of SUBLIGANA on acne-prone skin 4
6 SUBLIGANA 8-26% - 39% - 41% ** % % Control SUBLIGANA,67% TM 2% rlistat (1µM) Control SUBLIGANA TM,123% Control ** SUBLIGANA TM,123% Student s test: *p<.5, **p<.1, p<.1 Student s test: ns>.5,*p<.5, **p<.1, p<.1 Fig.1: Lipase activity inhibition (%) Fig.2a: IL-1 stimulation (%) Fig.2b: IL-12 inhibition (%) STHING ACTIVITY SUBLIGANA significantly inhibits lipase activity (Fig.1, Protocol I) & up & down regulates anti & pro-inflammatory factors Interleukin-1 (IL-1) & Interleukin-12 (IL-12) (Fig.2a&b, Protocol II). Protocol I: In-tubo test with pancreatic lipase enzyme. 2h application with SUBLIGANA TM or reference rlistat. Radioactive evaluation by counting. Ref study Bioalternatives GT Protocol II: In-vitro test on human peripheral blood mononuclear cells (PBMCs). 24h application with/without SUBLIGANA TM ; inflammation induction with LPS (Lipopolysaccharide) at 1 μg/ml. Evaluation by Elisa assay on IL-1 & IL-12. Ref study Biolaternatives GT TBARs production % Control,2%,6%,185%,55%,16%,5% Trolox 1mM SUBLIGANA TM ANTIXIDANT ACTIVITY SUBLIGANA significantly inhibits lipids peroxidation (Fig.3, Protocol III). Protocol III: In-vitro test on phosphatidylcholine micelles incubated with/without SUBLIGANA TM. Lipid peroxidation induced with Hemin. TBA (Thiobarbituric acid) is added to interact with MDA (Malonaldehyde) forming TBARs (Thiobarbituric acid reactive substances). Evaluation of the TBARs production by fluorescence measurement. Ref study Bioalternatives GT Fig.3: Lipids peroxidation inhibition (%) DERMPURIFYING EFFECT 6 5 SUBLIGANA significantly inhibits P. acnes proliferation (Fig.4, Protocol IV). 4 3 Protocol IV: In-tubo test on Propionibacterium acnes. Application with SUBLIGANA TM at 37 C for 48h. Evaluation by enumeration of micro-organisms at h, Control,1% SUBLIGANA TM,5% SUBLIGANA TM 2 4h, 8h, 24h & 48h. Ref study Amatsiavepharm 93484/1. 1% SUBLIGANA TM 1 T 4h 8h 24h 48h Time (hours) 5 Fig.4: Log of the bacterial enumeration
7 IN-VIV TEST: PRTCL N TEENAGERS WITH ACNE-PRNE SKIN Strong clinical efficiency: up to -34% of whiteheads at D28 12 ANTI-IMPERFECTINS EFFECT 8-19%* SUBLIGANA significantly decreases the number of whiteheads vs placebo (-34%*) and vs D (-19%*) at D Protocol V: In-vivo test on teenagers with acne prone skin. Test was conducted on 2 groups of 22 teenagers with greasy, brilliant & acne-prone skin. 28 days double-blind study vs Placebo. Product applied twice a day on all the face. Evaluation by counting the number of whiteheads at D28. Ref study Dermscan 14E1282. D D28 D D28-34%* D D28 * paired t-test Fig.5a: Whiteheads reduction at D28 (%) VISIBLE IMPRVEMENT Visible improvement of acne-prone skin after 1 month application on volunteer 7 (Mirroring effect on an hemi-face) & volunteer 23. Volunteers 7 (up) & 23 (down), at D & D28 Addressing blemished skins, SUBLIGANA showed a significant whiteheads reduction. It associates soothing effect (lipase inhibition, IL-1/IL-12 regulation), antioxidant effect (lipid peroxidation protection) & dermopurifying effect against Propionibacterium acnes. 6
8 SUBLIGANA TM Characteristics INCI Name: Glycerin - Aqua/Water - Harungana Madagascariensis Extract Solubility: hydrosoluble Recommended 1 to 2% concentration: Formulation: Preservative: easy to formulate none Patent pending: European Patent application submitted Approvals: * CHINA CMPLIANT* * *China Compliant: all INCI names are listed on the IECIC list of June 215. *ECCERT Cosmetics: raw material approved by ECCERT GREENLIFE, in conformity with Ecocert natural and organic standard. *CSMS Approved: raw material approved by ECCERT GREENLIFE, in conformity with Cosmos standard. Nota : The analytical specifications warranted are only those mentioned on the certificate of analysis supplied with each delivery of the product. Except as set forth above, SEPPIC* makes no warranties, whether express, implied of statutory, as to the product which is the subject of this document. Without limiting the generality, of the foregoing, SEPPIC* makes no warranty of merchantability of the product or of the fitness of the product for any particular purpose. Buyer assumes all risk and liability resulting from the use or sale of the product, whether singly or in combination with other goods. The information set forth herein is furnished free of charge and is based on technical data that SEPPIC* believes to be reliable. Its is intended for use by persons having technical skill and their own discretion and risk. Since conditions of use are outside SEPPIC* s control, SEPPIC* makes no warranties, express or implied, and assumes no liability in connection with any use of this information. Nothing herein Is to be taken as a license to operate under or a recommendation to infrige any patents. Air Liquide Healthcare is a world leader is medical gases, home healthcare, hygiene products and healthcare specialty ingredients. It aims to provide customers in the continuum of care from hospital to home with medical products, specialty ingredients and services that contribute to protecting vulnerable lives. *SEPPIC being: SEPPIC S.A. 22 Terrasse Bellini Paris La Défense 9286 Puteaux cedex - France Tel () info.seppic@airliquide.com SEPPIC Castres FRANCE Tel () Head ffice 75, quai d rsay 757 Paris FRANCE 215 SEPPIC SEPPIC is a subsidiary of the AIR LIQUIDE group SEPPIC Inc. USA Tel us.seppic@airliquide.com SEPPIC Japan ffice JAPAN Tel japan.seppic@airliquide.com SEPPIC Poland ffice PLAND Tel poland.seppic@airliquide.com SEPPIC Italia Srl ITALY Tel italy.seppic@airliquide.com SEPPIC Brasil BRASIL Tel brasil.seppic@airliquide.com SEPPIC Asia Singapore SINGAPRE Tel singapore.seppic@airliquide.com SEPPIC Dubaï ffice UNITED.ARAB.EMIRATES Tel (4) dubai.seppic@airliquide.com SEPPIC GmbH GERMANY Tel () germany.seppic@airliquide.com SEPPIC Colombia SAS CLMBIA Tel colombia.seppic@airliquide.com SEPPIC India INDIA Tel india.seppic@airliquide.com SEPPIC China CHINA Tel (21) china.seppic@airliquide.com BITECHMARINE FRANCE Tel () contact.biotechmarine@airliquide.com Photos: Serdex, Fotolia / Medical illustrations: Hélène Fournié / Brochure design: BiotechMarine - Hélène Fournié / Brochure version: 787/GB/1/February 217 Serdex joined Seppic in january 217. An activity of SERDEX S.A.S. 1 avenue Normandie Niemen LNS (FR) Phone: + 33 ( ) contact-serdex@airliquide.com seppic.com
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