January 22 nd to 26 th, 2008

Transcription

1 Advanced theoretical and practical training course on Hearing in Mammals January 22 nd to 26 th, 2008 F ro m M o l e c u l e s t o A u d i t o r y P h y s i o l o g y ORGANIZERS Tobias Moser FACULTY Frank, T. Hoch, G. Khimich, D. Kubisch, C. Kurt, S. Liberman, M.C. Meyer, A. Moser, T. Müller, M. Neef, A. Nouvian, R. Oliver, D. Pangrsic, T. Riedel, D. Reisinger, E. Taschenberger, H. Wolf, F. LOCATION University of Göttingen (Medical School) Max Planck Institute for Biophysical Chemistry Current auditory research covers a large spectrum of questions: from addressing the molecular nature of basic sensory mechanisms such as mechanoelectrical transduction and ribbon synapse function to asking how complex neuronal tasks such as signal detection in noisy environments are achieved. Accordingly, the work involves various disciplines reaching from molecular biology to psychophysics. Here we will provide an overview about and hands-on experience in contemporary auditory research. The course primarily targets PhD students and is limited to 12 participants to enable efficient instruction. During this one-week course we will focus on the peripheral and central auditory system of rodents. The course concludes with a symposium on Neural Coding in the Auditory System. Topics: Introduction: anatomy, physiology and pathology Genetic approaches to hearing and deafness Microdissection, expression profiling, transfection of the inner ear Recordings of gross responses in the auditory system Cochlear micromechanics/outer hair cell function Hair cell synapse Auditory nerve and brainstem Inferior colliculus and auditory Cortex Practical training: Dissection of the inner ear Expression profiling in the cochlea Recordings of gross responses in auditory physiology Hair cell physiology Recording from single spiral ganglion neurons Synaptic physiology in the auditory brainstem, calyx of Held Application: Please send your application including your CV, an abstract summarizing your current research and a short statement why you would like to attend the course, to abock2@gwdg.de (application deadline:12th of December 2007; max. 12 participants; no registration fee for the course required).

2 Tuesday, Welcome and Introduction Introduction into the Cochlea Introduction into central auditory system Tobias Moser Holger Taschenberger Recordings of gross responses in Auditory Physiology (endocochlear potential, endolymph composition, microelectrode recording, gross potential recordings) Technical implementation (general principle, speakers, microphones, calibrations) Practical training Recordings of gross responses in mice I (Otoacoustic emissions, Auditory brainstem responses, Auditory Steady State responses) Coffee break Practical training Recordings of gross responses in mice II (Otoacoustic emissions, Auditory brainstem responses, Auditory Steady State responses) Tour to Göttingen InnerEarLab 02B4 InnerEarLab 02B4

3 Wednesday, Basic electrophysiology of hair cells (patch clamp, ionic distribution, Nernst equation, membrane potential, mechanoelectrical transduction, potassium currents) Micromechanics/Outer Hair Cell function (Micromechanics, non-linear capacitance, electromotility, prestin and associates, the amplifier quest) Sound coding at the hair cell synapse: (synaptic ribbons, vesicle pools, Ca2+ currents, capacitance, imaging, electron microscopy, glutamate receptors, transporters, spike generation) Tobias Moser Class room Practical training Hair cell physiology I (transduction, basolateral currents) Andreas Neef Coffee break Practical training Hair cell physiology II (electromotility, synaptic transmission) Andreas Neef

4 Thursday, Expression profiling: PCR-based strategies and Protein detection Genetic approaches to hearing and deafness Christian Kubisch Practical training Molecular approaches I (Microdissection, expression profiling: PCR, imunohistochemistry, organotypic culture) Lunch Practical training Molecular approaches II (Microdissection, expression profiling: PCR, immunohistochemistry, organotypic culture) Coffee break Practical training Imaging (confocal microscopy; electron microscopy) Tina Pangrsic Tina Pangrsic Alexander Meyer Dietmar Riedel Thomas Frank FILM, EM-Facility MPIbpc

5 göttingen Opening lecture M. Charles Liberman, Boston How different are the afferent connections within and among cochlear inner hair cells? Marcus Müller, Tübingen Alexander Meyer, Göttingen Biophysics and statistics of the hair cell synapse Andreas Neef, Göttingen Peter Heil, Magdeburg Coding in the auditory pathway Wiebe Horst, Groningen, Göttingen Werner Hemmert, Munich!!!! Benedikt Grothe, Munich Dmitry Bibitchkov, Göttingen Simone Kurt, Ulm!!!!! Closing lecture Ian Russell, Brighton UNIVERSITY OF GÖTTINGEN JANUARY 2008 Friday, Auditory nerve Charles Liberman Auditory brainstem Holger Taschenberger Coffee break Inferior Colliculus and Auditory Cortex Modeling the auditory system Practical training In vitro and in vivo single unit recordings (auditory nerve, calyx of Held, inferior colliculus) Simone Kurt Fred Wolf Simone Kurt Holger Taschenberger InnerEarLab, 02B4 and 0D3 MPIbpc Saturday, Minisymposium Neural Coding in the Auditory System Schedule Speakers Location & M.C. Liberman, Boston M. Müller, Frankfurt/Tübingen A. Meyer, Göttingen A. Neef, Göttingen P. Heil, Magdeburg W. Horst, Groningen N. Strenzke, Göttingen W. Hemmert, Munich D. Bibitchkov, Göttingen B. Grothe, Munich S.Kurt, Ulm I. Russell, Brighton Lecture Hall 04 Symposium, January 26 th, 2008, 9:00-18:00 Neural Coding in the Auditory System Universitätsklinikum, Robert-Koch-Straße 40, Hörsaal 04 Organisation: Tobias Moser, Fred Wolf, Tobias Niemann, Andreas Bock Reading assignments: Chapter 'Hearing' in: Principles of Neural Science (2000) E.R. Kandel, J.H. Schwartz, T.M. Jessel (eds) Fettiplace R, Hackney CM (2006) The sensory and motor roles of auditory hair cells. Nat Rev Neurosci. 7: Moser T, Brandt A, Lysakowski A (2006) Hair cell ribbon synapses. Cell Tissue Res 326: von Gersdorff H, Borst JG (2002) Short-term plasticity at the calyx of held. Nat Rev Neurosci. 3: Grothe B, Klump GM (2000) Temporal processing in sensory systems. Curr Opin Neurobiol. 10: Dallos P, Fakler B (2002) Prestin, a new type of motor protein. Nat Rev Mol Cell Biol 3: Robles L, Ruggero MA (2001) Mechanics of the mammalian cochlea. Physiol Rev 81: