Akoshine Active Coral THE MEDITERRANEAN RED GOLD

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1 Akoshine Active Coral THE MEDITERRANEAN RED GOLD

2 Corallium Rubrum CLASSIFICATION Phylum: Celenterati Class: Antozoi Underclass: Octocoralli Order: Gorgonacei Family: Corallidi. Its hard, calcareous, red skeleton is responsible for its typical shape and structure and the high commercial value. HISTORY AND TRADITION The origin of Coral halfway between the realms of animal, vegetal and mineral has been told through legend. Since antiquity it has been gathered and crafted in jewellery by the inhabitants of the Mediterranean. SYMBOLISM AND METAPHISICAL PROPERTIES It it considered a universal amulet/talisman, to to bring great fortune and luck.

3 Akoshine Active Coral CHARACTERISTICS INCI NAME: Coral Powder ORIGIN: Mediterranean Sea SOURCE: first-quality discards of jewellery APPEARANCE: fine powder COLOUR: pink AVERAGE PARTICLE SIZE: < 5 microns HEAVY METALS: < 1 ppm RECOMMENDED DOSAGE : 0.1-2%.

4 Akoshine Active Coral MICROELEMENTS We have verified the amount of trace elements contained in our powder by atomic absorption: Minerals & trace elements Units ** iron copper zinc manganese lead mercury cadmium 38.7 ppm 2.44 ppm 6.5 ppm 3.24 ppm < 0.2 ppm < 0.1 ppm < 0.01 ppm ** indicative only, not significant because of the limited number of samples used for chemical analysis

5 Akoshine Active Coral OPTICAL PROPERTIES We have investigated the optical properties of our powder through ellipsometry technic. The observed index of refraction (1.51) can be compared to that characterizing glass (1.4) and crystal (1.56).

6 Akoshine Active Coral SURFACE AREA We have analyzed the surface area of our powder through BET method. BET surface area = m 2 /g The data obtained is superior to the main BET surface area of other fine cosmetic powders used for filling wrinkles, hiding imperfection and promoting radiance. BET surface area of silica powder is 9 m 2 /g

7 Akoshine Active Coral PARTICLE SIZE Akoshine Active Coral is a very fine, pink, velvety powder obtained through a patented manufacturing process - nature-friendly - without use of solvents and preservatives. The mean particle size has been controlled through SEM analysis. Mean particle size < 5 microns

8 Akoshine Active Coral EFFICACY TESTS We have investigated the ability of our powder to stimulate cell proliferation and total protein synthesis in human keratinocytes through MTT and Bradford tests: cell proliferation: + 39,3% protein synthesis: + 21,8%

9 Akoshine Active Coral PERFORMANCE SENSORY EVALUATION IN VIVO Blind study with a face cream containing 2% of Akoshine Active Coral applied twice/day for 1 month. The protocol was carried on through the evaluation of 5 selected parameters by 30 female volunteers, with different type of skin and aged PARAMETERS Radiance Smoothness Mattifying effect Firmness Filler effect EFFECTS REPORTED BY VOLUNTEERS Enhances the radiance and promotes a fresh and healthier look Smoothes the skin, refines the texture, attenuates fine lines and wrinkles Reduces shine with a powdery and velvety feel Improves tone and elasticity The skin looks redensified, more compact % 70% 65% 68% 71% 73% 100% of panellists observed a visible improvement in the appearance of the skin. Akoshine Active Coral is the natural choice for a global rejuvenating effect. It enhances radiance and suppleness while promoting a better skin texture and a more uniform and fresh complexion. Akoshine Active Coral acts also as a sensorial agent, for a velvety and matte finish and as a texturing agent for a unique ultra-soft feel*. * sensory evaluation

10 Akoshine Active Coral BENEFITS Natural source of essential trace elements Immediate anti-ageing effect by filling in fine lines and small wrinkles Proven ability to stimulate cell metabolism and renewal Skin brightener and radiance enhancer Green nature friendly sustainable Preservative-free Positive consumer perception Ideal for all natural-mineral formulations Non-irritant (36-hrs PATCH TEST)

11 AKOACTIVE CORAL MILK THE RED GOLD FROM THE MEDITERRANEAN SEA FOR A LUXURY SYNERGY ACTIVE

12 CHARACTERISTICS INCI NAME: Water, Glycerin, Sodium Hyaluronate, Coral Powder, Xanthan gum, Sodium Benzoate, Sorbic Acid ORIGIN: Mediterranean Sea SOURCE: Corallium Rubrum (first-quality discards of jewellery) APPEARANCE: milky suspension COLOR: pink ODOR: characteristic ph: 6-8

13 EFFICACY TESTS Fibroblasts are the main component in the dermis, the deeper layer of the skin, and are responsible for the synthesis of extracellular matrix proteins such as collagen and elastin. We tested the effect of AKOACTIVE CORAL MILK on the stimulation of the cell cycle and total protein synthesis on human fibroblasts. AKOACTIVE CORAL MILK effects on human fibroblasts: protein synthesis AKOACTIVE CORAL MILK effects on human fibroblasts: cell proliferation Coral milk 0.05% Coral milk 0.1% +13 % % Coral milk 0.05% Coral milk 0.1% % +4.8 % +7 % +2.6 % 24 hrs 48 hrs Bradford assay 48 hrs Cell viability assay (MTT)

14 THE CLAIM AKOACTIVE CORAL MILK, holds the rejuvenating benefits of AKOSHINE ACTIVE CORAL which supports proliferation of skin cells thanks to the high content of minerals, trace elements and unique peptides. In synergy with the proven anti-ageing power of Sodium Hyaluronate this ingredients enhances skin trophism, firmness and renewal. SUGGESTED APPLICATIONS Anti-ageing skin-care, functional make-up, sun-care, products for the scalp, SPA products with luxury-mineral claim. RECOMMENDED DOSAGE: 0,5-3%.

15 THANKS FOR YOUR ATTENTION