* * 43 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. //, No. /,,./,.3 (,**2)

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1 43 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. //, No. /,,./,.3 (,**2) 245 / * * * 0 1 bo * 2 +* Immunostimulating Activity and Molecular Weight Dependence of an Arabinoxylan Derived from Wheat Bran Manami Monobe, Mari Maeda-Yamamoto, Yuki Matsuoka*, Akihiro Kaneko* and Shigeru Hiramoto* National Institute of Vegetable and Tea Science, NARO * Health Care Research Center, Nisshin Pharma, INC. Arabinoxylan, the main component of hemicellulose in plant cell walls, is known to be not only a dietary fiber but + also an immunomodulator. Here, we investigated the immunostimulating activity and the molecular weight dependence of an arabinoxylan derived from wheat bran. Wheat bran mixed with water was treated by autoclaving Fig. + under +. 2 atm at +,* for +/ min followed by treatment with hemicellulase at /* for,* min. Distilled water was,** g /* +.. L- /* added, followed by centrifuging and lyophilizing. We +.. L - used phagocytic activity of macrophage-like cells as an indicator of immune function activation. The immuno- - stimulating activity of arabinoxylan from wheat bran 0** ml was higher than that arabinoxylan from corn husk and TOMY SS- -,/ +,* / rice bran and its activity was attributable to the fraction with a molecular weight of over / kda. (Received Jan.,,,,**2 ; Accepted Feb. +-,,**2) + L RS ; Keywords : arabinoxylan, immunostimulating activity, molecular weight *., g /*,* 3* : +/ +1 g Fig. + +* g +** ml /* + /* ; Fraction + 2/ + +. /* 2/ ; Fraction, 2/ ; Fraction - 2/ ODS.,22/*+,103 * -/02/++ / - + VARIAN Bond Elut C+2 Corresponding author monobe@a# rc.go.jp Fraction. Fraction /

2 246 // /,**2 / 44 Fig. + Production scheme for arabinoxylan from wheat bran Fig., Fractionation of wheat arabinoxylan by EtOH precipitation and ODS column Fig., + g. ml Sephacryl S-+** HR GE Healthpullulan care,. 2 cm, -0 cm +/ ml HPLC : TOSOH G,*** SWXL : Fraction * ++ + mlmin : RI standard P- 2,, Shodex, /. 3.*. kda, mg, moll */. ml +*/, HPLC : Shodex SUGAR KS 2*+ : *./ mlmin, : RI, /* ++ +* FBS Invitrogen Life Tech- nologies, Carlsbad, CA, U.S.A. +,* nm

3 45 : 247 Fig. - Phagocytic activities by arabinoxylan from wheat bran and corn husk Phagocytosis activity in the absence of polysaccharides (control) is normalized to +**. Values are the means SD, n-. * p*.*+ and ** p*.**+ versus the control. # p*.**+ versus + g/ml of arabinoxylan from corn husk. RPMI+0.* Gibco- : +** :,1 :.* Invitrogen, Burlington, Ontario, Canada 1,. 2 kda, *., /** kda HL0* ATCC CCL-,.*.2 Falcon -/++12 ; BD Biosciences, Franklin Lakes, HL0* NJ, U.S.A. /,./ +* cellswell,/* L YG HL0* Polysciences, Inc., Warrington, PA, U.S.A.,/ L,/ L CO Fig /, +0 -, PBS Beckman Coulter, *+. gml Fullerton, CA, U.S.A. LPS + gml + g/ml ; Calbiochem, Darmstadt, Germany +** gml +** +** gml Fig. - IP ; +*** ; DK Fig.. bo Fig. / - kda Fr., / kda Fr., BonferroniDunn / / kda,*,+,, *,.+. kda Fig. 0 2* /* 2*,* 3/ / kda :

4 248 // /,**2 / 46 Fr. + Fr., *. 0 /** kda 1* Fr., Fr. + Fr. +, ,3 / kda +* M +, +. /** kda bo +/ Fig.. Phagocytic activities by immune enhancing polysaccharides The immune enhancing polysaccharides were added at final concentrations of +* g/ml, except for LPS. - Phagocytosis activity in the absence of polysaccharides (control) is normalized to +**. Values are HL the meanssd, n-. ** p*.**+ versus the control. # 0* p*.**+ versus the arabinoxylan from wheat bran. Fig. / Phagocytic activities by various molecular weight fractions The arabinoxylans from wheat bran were added at final concentrations of +** g/ml. Phagocytosis activity in the absence of polysaccharides (control) is normalized to +**. Values are the meanssd, n-. * p*.*+ and ** p*.**+ versus the control.

5 47 : 249 Fig. 0 Phagocytic activities by high molecular weight fractions The arabinoxylans from wheat bran were added at final concentrations of +** g/ml. Phagocytosis activity in the absence of polysaccharides (control) is normalized to +**. Values are the means SD, n-. ** p*.**+ versus the control. # p*.**+ versus the Fr. +. / kda + Lactobacillus gasserioll,1+0 LG,+ Helicobacter pylori H. pylori ,**+, Miyaji, M., Yasue, N., Hayakawa, T., Higashi, K., Kimura, M., Tsukada, K., Takeuchi, T., Uchida, M., Kume, A. and Kohno, T., Yogurt containing lactobacillus gasseri OLL,1+0 (LG,+) ameliorates gastric mucosal inflammation in helicobacter pylori infected patients with upper gastrointestinal symptoms, Jpn. Pharmacol. Ther., -., (,**0) /,**/. L. NK Prev. Med.,.*, /23 /3. (,**/). / /, /2. /3-,**/ 0 / Vetvicka, V., Terayama, K. and Mandeville, R., Pilot Study : Orally-administered yeast b+, --glucan prophylactically protects against anthrax infection and cancer in mice, J. Am. Nutraceut. Assn., /, +0,* (,**,). 2 New Food Industry,.,, 0 ++ (,***). 3 Arabinoxylan Compound MGN ,**+ +* Ghoneum, M. and Matsuura, M., Augmentation of macroenhancing phage phagocytosis by modified arabinoxylan rice bran (MGN--/biobran). Int. J. Immunopathol. Pharmacol., +1,,2-,3, (,**.). ++ Monobe, M., Ema, K., Kato, F., Hirokane, H. and Maeda- Yamamoto, M., Technique for screening immunevitamin polysaccharides in food using +,/, -dihydroxy- D- -di# erentiated HL0* cells. J. Agric. Food Chem., //,,/.-,/.1 (,**1). +, Kunisawa, J. and Kiyono, H., A marvel of mucosal T cells and secretory antibodies for the creation of first lines of defense. Cell Mol. Life Sci., 0,, +-*2 +-,+ (,**/). +-,3 +0,0,**0 +. +,1 -+3-,0,**1 +/ Suzukia, I., Tanakaa, H., Kinoshitaa, A., Oikawab, S., Osawab, M. and Yadomaea,T., E# ect of orally administered b-glucan on macrophage function in mice, Int. J. Immunopharmacol., +,, 01/ 02. ( +33* ).,* +,,,*, +-