Certificate of Analysis
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1 Sample Name: Black Cumin Seed Oil Covance Sample: Project ID PO Number Lot Number 1 Sample Serving Size Description ACTIVAT_PR N/A 207 ml Oil Pressed from Black Receipt Date Receipt Condition Login Date Storage Condition Number Composited 2 24-Apr-2013 Ambient temperature 28-Apr-2013 Online Order (+/- 3) degrees Celsius Analysis Result Result per Serving Result per Daily Value from Fat Fatty Acid Profile Saturated Fatty Acids (Acid Form) Monounsaturated Fatty Acids ( Acid Form) Polyunsaturated Fatty Acids ( Acid Form) Trans Fatty Acids (Acid Form) Total Fatty Acids Cholesterol Cholesterol Carbohydrates Total Carbohydrates Total Dietary Fiber Total Dietary Fiber Sugar Profile Fructose Glucose Sucrose Lactose Maltose Galactose Total Sugar Protein (N x 6.25) Dumas Method Protein Total Vitamin A * Total Vitamin A Vitamin A as Retinol Vitamin A from Retinol Vitamin A from Carotenes * Vitamin A From Carotene 858 Cal/100g 840 Cal/100g 14.4 g/100g 22.1 g/100g 52.6 g/100g g/100g 93.3 g/100g 1.40 mg/100g 3.6 g/100g <1.00 g/100g 1.04 g/100g <0.35 IU/g <100 IU/100g <35.0 IU/100g 1640 Cal/Serving size 1600 Cal/Serving size 27.5 g/serving Size 42.2 g/serving Size 100 g/serving Size g/serving Size 178 g/serving Size 2.7 mg/serving Size 6.9 g/serving Size <1.91 g/serving Size 1.98 g/serving Size <67 IU/Serving size <191 IU/Serving size <66.8 IU/Serving size 137 % Daily Value US 274 % Daily Value US 0.89 % Daily Value US 2.3 % Daily Value US <7.63 % Daily Value US <3.82 % Daily Value US <1.34 % Daily Value US Page 1 of 5
2 Sample Name: Black Cumin Seed Oil Covance Sample: Project ID PO Number Lot Number 1 Sample Serving Size Description ACTIVAT_PR N/A 207 ml Oil Pressed from Black Receipt Date Receipt Condition Login Date Storage Condition Number Composited 2 24-Apr-2013 Ambient temperature 28-Apr-2013 Online Order (+/- 3) degrees Celsius Analysis Result Result per Serving Result per Daily Value Carotenes Beta Carotene Vitamin C (by HPLC) Vitamin C Elements by ICP Emission Spectrometry Calcium Iron Sodium Alpha Tocopherol Vitamin E Ash Ash Moisture Moisture Specific Gravity * Density Peroxide Value Peroxide Value < mg/100g <1.0 mg/100g 25.4 mg/100g 1.72 mg/100g <2.46 mg/100g 1.09 mg/100g 0.19 g/100g 1.83 g/100g g/ml 0.41 Meq/100g < mg/serving Size <1.9 mg/serving Size 48.5 mg/serving Size 3.28 mg/serving Size <4.70 mg/serving Size 2.08 mg/serving Size 0.36 g/serving Size 3.49 g/serving Size <3.2 % Daily Value US 4.85 % Daily Value US 18.2 % Daily Value US <0.196 % Daily Value US Alpha Tocopherol (LCE1_S:11) Cort, W. M., Vincente, T. S., Waysek, E. H., and Williams, B. D., Journal of Agricultural Food Chemistry, 31: (1983). (Modified) Speek, A. J., Schijver, J., and Schreurs, W. H. P., Journal of Food Science, 50: (1985). (Modified) McMurray, C. H., Blanchflower, W. J., and Rice, D. A., Journal of the Association of Official Analytical Chemists, 63: (1980). (Modified) Ash (ASHM_S:5) Official Methods of Analysis of AOAC INTERNATIONAL, 18th Ed., Method , AOAC INTERNATIONAL, Gaithersburg, MD, USA, (2005). (Modified) Page 2 of 5
3 (CALC:4) United States Department of Agriculture, "Composition of Foods" Agricultural Handbook, No. 8, pp , (1975). from Fat (CFAT:3) United States Department of Agriculture, "Composition of Foods" Agricultural Handbook, No. 8, pp , (1975). Carbohydrates (CHO:7) United States Department of Agriculture, "Energy Value of Foods", Agriculture Handbook No. 74, pp. 2-11, (1973). Carotenes (CAR1_S:14) Official Methods of Analysis of AOAC INTERNATIONAL (2005), 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method , (Modified). Quackenbush, F. W., Journal of Liquid Chromatography, 10: , (1987). (Modified) Cholesterol (CHOK_S:8) Official Methods of Analysis of AOAC INTERNATIONAL 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA,(2005), Official Method (Modified) Elements by ICP Emission Spectrometry (ICP_S:17) Official Methods of Analysis of AOAC INTERNATIONAL, 18th Ed., Method and , AOAC INTERNATIONAL, Gaithersburg, MD, USA, (2005). (Modified) Fatty Acid Profile (FPT_S:17) Official Methods of Analysis of AOAC INTERNATIONAL 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, (2005),Official Method (Modified) Official Methods and Recommended Practices of the AOCS, Official methods Ce 1h-05 and Ce 2-66, The American Oil Chemists' Society, Champagn, IL, (2005) (Modified) Moisture (M100T100_S:5) Official Methods of Analysis of AOAC INTERNATIONAL, 18th Ed., Methods and , AOAC INTERNATIONAL, Gaithersburg, MD, USA,(2005). (Modified). Peroxide Value (PVFF_S:9) United States Pharmacopeia, Thirty-Fifth Revision, <401 Fats & Fixed Oils>, USP Convention, Inc., Rockville, MD (2012). Lipid extraction: Official Methods of Analysis of the AOAC International, 18th edition, (2005), AOAC International, Gaithersburg, MD. Official Method (modified). Page 3 of 5
4 Protein (N x 6.25) Dumas Method (DGEN_S:5) Official Methods of Analysis of AOAC INTERNATIONAL, 18th Ed., Methods and , AOAC INTERNATIONAL, Gaithersburg, MD, USA, (2005). (Modified) Specific Gravity (SPGP_S:5) United States Pharmacopeia, Twenty-Sixth Revision, <841>, United States Pharmacopeial Convention, Inc.: Rockville, Maryland (2003) Sugar Profile (SUGN_S:9) Mason, B. S., and Slover, H. T., "A Gas Chromatographic Method for the Determination of Sugars in Foods," Journal of Agricultural and Food Chemistry 19(3): (1971). (Modified) Brobst, K. M., "Gas-Liquid Chromatography of Trimethylsilyl Derivatives, Methods in Carbohydrate Chemistry," 6:3-8, Academic Press, New York, NY, (1972). (Modified) Total Dietary Fiber (TDFL_S:11) Official Methods of Analysis of AOAC INTERNATIONAL (2005) 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method (Modified) Total Vitamin A (VAT_CALC:10) Calculation on request. Vitamin A as Retinol (VALC_S:9) Official Methods of Analysis of AOAC INTERNATIONAL, 18th Ed., Methods , , AND , AOAC INTERNATIONAL, Gaithersburg, MD, USA, (2005). (Modified) Vitamin A from Carotenes (BCAV_S:6) Official Methods of Analysis of AOAC INTERNATIONAL, 18th Ed., Methods and , AOAC INTERNATIONAL, Gaithersburg, MD, USA, (2005) Vitamin C (by HPLC) (CALL_S:5) Food Chemistry 94 (2006) (Modified). Journal of Analytical Chemistry (1992) 342: (Modified). (s) 3301 Kinsman Blvd Madison WI x4170 Released on Behalf of Covance by Lori Ross - Associate Director Testing Cert # Page 4 of 5
5 These results apply only to the items tested. This certificate of analysis shall not be reproduced, except in its entirety, without the written approval of Covance. Page 5 of 5
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