Practical Histology o
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1 Practical Histology o 1.. Contents: Histology of the : Stomach Esophagus Small intestine Large intestine Liver Gallbladder Exocrine pancreas Spleen GNT Block
2 Things you need to know before the exam : o The pictures in the exam will be the same as the ones included in the slides. o Do t tr to take short uts duri g the e a so avoid usi g a reviatio s so you don t lose marks. o Please keep in mind that this work is done by students, so if there are any mistakes please inform us. o This work is not by any means a reference. o Please study hard and don t worry the exam will be easy!! Identify the *م جدا جدا جدا جدا تبربط ن الص ر ب لمع مة ب الختب ر بيجي سؤال : مالحظة هذا اكثر سؤال يجي structure مثال stomach انه يجي ص ره لل, * الدكت ر نبه ع ى شيء يخط ن فيه كثير من الطال Mucosa and submucosa,ect هن الز تكت الاليرز ك م ة Mention the 4 layers ي ل Epithelium, lamina propria,ect هن تكت subdivisions of the mucosa بس لمن ي ل
3 Esophagus 1- Mucosa: Epithelium Non-Keratinized Stratified Squamous Epithelium. Lamina propria Muscluaris Mucosae 2- Submucosa Submucosal Glands Meissner's plexuses 3- Muscularis Externa Inner circular M e teri ple uses Auer a h s Outer longitudinal layer. 4- Serosa ( in abdominal part ) or Adventitia ( in cervical and thoracic parts ) 4
4 1- Mucosa: Fundus of the stomach Fundic Glands Epithelium: simple columnar epithelium Lamina propria Muscluaris Mucosae Fundic Glands: Parietal cells Peptic cells Mucous neck cells Stem cells Enteroendocrine cells Short pits 2- Submucosa NO submucosal glands meissner's plexuses 3- Muscularis Externa Inner oblique Middle circular M e teri ple uses Auer a h s d) Outer longitudinal 4-Serosa Peptic cells (basophili Parietal cells ( acidophilic appears pink in colour )
5 1- Mucosa: Pylorus of the stomach Epithelium: simple columnar epithelium Pyloric Glands : Mucous neck cells Few parietal cells Their pits are deep Stem cells No peptic ( chief ) cells Enteroendocrine cells Lamina propria d) Muscluaris Mucosae 2- Submucosa NO submucosal glands Meissner's plexuses 3- Muscularis Externa Inner circular M e teri ple uses Auer a h s Outer longitudinal 4-Serosa
6 Villus Intestinal villi and crypts Crypt 1-Villi: Covering epithelium Central core of C.T contains : Lymphocytes Lacteal 2-Crypts : Columnar absorptive cells. Goblet cells Enteroendocrine (EE) cells d) Paneth cells ( at the base of the crypt ) e) Stem cells Paneth cells Paneth cells
7 Duodenum 1- Mucosa (Shows villi and crypts) : Epithelium lining : Villi : 1. simple columnar epithelium (brush border) with goblet cells crypts of lieberkuhn : 1. Paneth cells 2. Goblet cells 3. Simple columnar epithelium (brush border) 4. Stem cells 5. M cells over lymphoid nodules Lamina propria Muscluaris Mucosae 2- Submucosa Bru er s gla ds (seromucus glands) meissner's plexuses 3- Muscularis Externa Inner circular M e teri ple uses Auer a h s Outer longitudinal 4-Serosa Recommended by the doctor Brunner's glands the centre is whitish -
8 1- Mucosa: Ileum Epithelium lining (Similar to the deudenum) : simple columnar epithelium with goblet cells M cells over lymphoid nodules Lamina propria Has lymphoid nodules (Peyer's patches) that extend to the submucosa. Muscluaris Mucosae 2- Submucosa 3- Muscularis Externa: Inner circular Myenteric plexuses ( Auer a h s ) Outer longitudinal 4-Serosa Peyer's patches Peyer's patches
9 Ileum Recommended by the doctor
10 1- Mucosa (Shows only crypts) : Epithelium lining : simple columnar epithelium with goblet cells Lamina propria : Contains frequent lymphatic nodules (White circle) Muscluaris Mucosae NO VILLI 2- Submucosa 3- Muscularis Externa Inner circular M e teri ple uses Auer a h s Outer longitudinal (has Teniae coli). 4-Serosa (has appendices epiploicae). Cells lining the crypts of the colon are: Surface columnar absorptive cells. Goblet cells. Enteroendocrinecells. Stem cells. M-cells. Colon
11 Appendix 1- Mucosa: Epithelium lining (Similar to the Colon) : simple columnar epithelium with goblet cells Lamina propria Contains lymphoid nodules Muscluaris Mucosae 2- Submucosa 3- Muscularis Externa: Inner circular Myenteric plexuses ( Auer a h s ) Outer longitudinal 4-Serosa
12 Liver 1- Features: Classical hepatic lobule. Central hepatic vein. Portal area. d) C.T. Mention four cells in the square: d) Hepatocytes. Ito Cells ( stellate cells ) Kuppfer Cells Cells Of Endothelium Reticular fibers in liver
13 Portal Area 1- Features: d) Branch of portal vein. Bile duct. Branch of hepatic artery C.T Mention four cells in the square: d) Hepatocytes. Ito Cells ( stellate cells ) Kuppfer Cells Cells Of Endothelium
14 Gallbladder 1- Mucosa: Epithelium lining (Similar to the Colon) : simple columnar epithelium Lamina propria Contains contains mucous glands in the neck of gall bladder. Muscluaris Mucosae 2- Muscularis Externa: Smooth muscle fibres are oriented in all directions M e teri ple uses Auer a h s 3- Adventitia except the fundus which is Serosa
15 Pancreas 1- Features: 1-Islets of Langerhans 2-Pancreatic acini : 3-Centroacinar cells: pancreatic acinar cells 2-Features of the pancreatic acinar cells : Basal part basophilic due to abundant rer Apical part acidophilic due to granules
16 Spleen 1- Features: White pulp Periarterial lymphatic sheaths (PALS): housing T lymphocytes. Lymphoid follicles (with germinal centers): housing B lymphocytes, plasma cells and macrophages. Central artery Red pulp: Splenic (pulp) cords (Bilroth s cord): Extravasated blood cells, plasma cells, B and T lymphocytes, macrophages, reticular cells and fibers. Splenic blood sinusoids.
17 Sublingual Gland Parotid Gland
18 SUMMERY
19 SUMMERY Colon Mucosa of colon : Crypts ONLY 2 LAYERS Serosa Simple columnar epithelium with goblet cells. Contains frequent lymphatic nodules -Inner circular layer. - -Outer longitudinal layer. (has Teniae coli) (has appendices epiploicae) Cells lining the crypts are: 1. Surface columnar absorptive cells. 2. Goblet cells. 3. Enteroendocrine Cells. 4. Stem cells. 5. M-cells Appendix Mucosa of Appendix : shallow Crypts ONLY Similar to the colon, Simple columnar epithelium with goblet cells. Contains lymphatic nodules. 2 LAYERS - - Serosa -Inner circular layer. - Outer longitudinal layer Mucosa of GallBladder : highly folded GallBladder Simple columnar epithelium contains mucous glands in the neck of gallbladder - - Srosa or adventitia
20 Thank you & good luck - Histology team Done by: Rana Barasain Omar Turkistani Faisal Alrabaii
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