WT;ATTAC-5 ** ** SkM

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1 SUPPLEMENTARY INFORMATION doi:.3/nature a WT;ATTAC- H/H;ATTAC- WT;ATTAC- H/H;ATTAC- 3 weeks months GFP p Inka relative expression 3 SkM Skel. Mus. FKBP- relative expression SkM Skel. Mus. relative expression SkM Skel. Mus. Supplementary Fig. : Validation of p Inka -speifi expression of the INK-ATTAC- tragene. a, GFP inteity of olleted from 3-week and -month-old untreated mie with the indiated genotypes. Sale ar, µm., qrt-pcr analysis of untreated -month-old mouse tissue analyzed for the relative expression of p Inka, FKBP-, and. Error ars, s.d.; n = 3 female mie per genotype. P <., P <..

2 p Inka relative expression -mo-old WT;ATTAC-3 -mo-old WT;ATTAC-3 FKBP- relative expression 7 3 Sk.M. SkM Brain Colon Lung Panreas Sk.M. SkM Brain Colon Lung Panreas Supplementary Fig. : p Inka and INK-ATTAC expression onurrently inrease with hronologial aging. Analysis of p Inka and INK-ATTAC expression in various tissues of - and -month-old WT;INK-ATTAC mie. Error ars, s.d.; n = 3 females per age group. P <., P <..

3 3 WT;ATTAC- Igfp H/H;ATTAC- 3 Igfp WT;ATTAC-3 H/H;ATTAC-3 Il- Igfp Il- Il- WT;ATTAC- Il- Igfp H/H;ATTAC- Il- Il- d H/H;ATTAC- GFP H/H;ATTAC- GFP+ 3 p pinka IL Supplementary Fig. 3: Tissues expressing the INK-ATTAC- tragene display elevated indiators of senesene. a, Relative expression of senesene markers in the indiated tissues of -month-old BuR H/H ;INK-ATTAC- and WT;INK-ATTAC- mie as measured y qrt-pcr. All inreases are statistially signifiant (P <.)., Relative expression of senesene markers in the indiated tissues of 3-week-old WT;INK-ATTAC-3 and BuR H/H ;INK-ATTAC-3 mie as measured y qrt-pcr. There were no signifiant differenes., As in ut for 3-week-old WT;INK-ATTAC- and BuR H/H ;INK-ATTAC- mie. d, GFP + and GFP ell populatio from of -month-old BuR H/H ;INK-ATTAC- mie analyzed for relative expression of various senesene markers y qrt-pcr. All inreases are statistially signifiant (P <.). Error ars, s.d.; n = 3 female mie per genotype

4 Fier diameter (μm) 3 3 Gastr o Ado minal Mouse (treatment) Weight (g) Fat (%) POV (g) Peri (g) (g) Mes (g) SSAT (g) Brown (g) H/H (+AP) H/H;ATTAC-3 ( AP) H/H;ATTAC- ( AP) H/H +AP H/H;ATTAC-3 AP H/H;ATTAC- AP Gastro ABD. (.9) 7. (.).7 (.) Running time (s). (.9). (3.). (.) 3 Running time (s). (.). (.).37 (.3) Distane (m). (.3). (.). (.) 7 3 Distane (m). (.3). (.).7 (.) Work (J).7 (.).3 (.). (.) Energy expended (J). (.).3 (.). (.).9 (.). (.). (.3) d e Cell diameter (μm) 3 Thikness (μm) Adipose Inguinal Dermis adipose Supplementary Fig. : AP7 treatment of BuR H/H mie does not delay p Inka -mediated agerelated phenotypes in the asene of INK-ATTAC. a, Mean musle fier diameters of gastronemius and adominal musles of -month-old AP7-treated BuR H/H mie and non-treated BuR H/H ;INK- ATTAC mie., Exerise aility of -month-old AP7-treated BuR H/H mie and non-treated BuR H/ H ;INK-ATTAC mie., Body and fat depot weights of -month-old AP7-treated BuR H/H mie and non-treated BuR H/H ;INK-ATTAC mie. Parentheses, s.d. d, Mean fat ell diameters in of -monthold AP7-treated BuR H/H and untreated BuR H/H ;INK-ATTAC mie. e, Suutaneous adipose layer thikness of -month-old AP7-treated and untreated BuR H/H ;INK-ATTAC mie. Color odes in, d and e are as indiated in a. Error ars indiate s.e.m. in a, d and e, and s.d. in. For all analyses n = female mie per genotype.

5 Genotype (treatment) BPM (SD) Sinus pauses % (SD) WT;ATTAC-3 ( AP) n=3.3 (.7). (.) H/H;ATTAC-3 ( AP) n=. (3.).37 (.3) H/H;ATTAC-3 (+AP) n=.7 (3.3).7 (.) WT;ATTAC- ( AP) n=3. (9.3). (.) H/H;ATTAC- ( AP) n=.7 (3.3). (.) H/H;ATTAC- (+AP) n= 7. (33.9). (.9) thikness (μm) d 3 3 H/H;ATTAC-3 AP H/H;ATTAC-3 +AP H/H;ATTAC- AP H/H;ATTAC- +AP p Inka relative expression H/H;ATTAC-3 +AP (n=) H/H;ATTAC-3 AP (n=) FKBP- relative expression H/H;ATTAC- +AP (n=3) H/H;ATTAC- AP (n=) H/H +AP (n=) relative expression Age (days) Supplementary Fig. : Age-assoiated traits of BuR hypomorphi mie that are p Inka - independent are not influened y learane of p Inka -positive ells. a, Measurement of heart sinus pause rhythm disturanes in mie of the indiated genotypes and treatments. Areviation: BPM, eats per min., Thinning of the aorta is not orreted y drug treatment in BuR H/H ;INK- ATTAC animals. Error ars, s.e.m.; n = female mie per genotype., qrt-pcr analysis of p Inka and INK-ATTAC expression in aortas of the indiated mie. Error ars, s.d.; n = 3 female mie per genotype per treatment. d, Survival urves of AP7-treated and untreated BuR H/H ;INK-ATTAC and AP-treated BuR H/H mie. We note that ardia stress tests performed on BuR H/H mie indiate that ardia failure is likely to e the primary ause of death of these animals.

6 3 WT;ATTAC- AP H/H;ATTAC- AP H/H;ATTAC- +AP p p Igfp Igfp 3 p p Supplementary Fig. : AP7 treatment of BuR H/H ;INK-ATTAC- animals redues p Inka -positive senesent ells. qrt-pcr analysis for indiators of senesene in (a), skeletal musle (gastronemius), () and eye () reveals that treatment of animals with AP7 leads to lower levels of senesene-assoiated markers. Error ars, s.d.; n = 3 female mie per genotype. All genes have a signifiant derease upon AP7 treatment (P <.).

7 mo WT;ATTAC- AP mo H/H;ATTAC- AP mo H/H;ATTAC- +AP after mo 3 GFP p Igfp 3 GFP p Supplementary Fig. 7: Late-life treatment of BuR H/H ;INK-ATTAC- animals redues p Inka - positive senesent ells. qrt-pcr analysis for indiators of senesene in (a) and skeletal musle (gastronemius) () reveals that treatment of animals with AP7 leads to lower levels of senesene-assoiated markers. Error ars, s.d.; n = 3 female mie per genotype. All genes (exept those marked with ) have a signifiant derease upon AP7 treatment (P <.).