Product Datasheet. Endothelin-1 Antibody (TR.ET.48.5) NB Unit Size: 100 ul. Store at -20C. Avoid freeze-thaw cycles.

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1 Product Datasheet Endothelin-1 Antibody (TR.ET.48.5) NB Unit Size: 100 ul Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 2 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/12/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Endothelin-1 Antibody (TR.ET.48.5) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer 100 ul 2.5 mg/ml Store at -20C. Avoid freeze-thaw cycles. Monoclonal TR.ET % Sodium Azide IgG1 Immunogen affinity purified PBS Page 1 of 5 v.20.1 Updated 2/12/2019 Product Description Host Mouse Gene ID 1906 Gene Symbol Species Reactivity Notes Immunogen EDN1 Human, Mouse, Rat, Porcine, Canine, Rabbit, Sheep Canine reactivity reported in scientific literature (PMID: ). Rabbit reactivity reported in scientific literature (PMID: ). Porcine reactivity reported in scientific literature (PMID: ). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Pure endothelin-1 conjugated to KLH. Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Radioimmunoassay Western Blot 1:100-1:1000, Flow Cytometry 0.5 ug, Immunohistochemistry 1:250, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry-Paraffin 1:250, Immunohistochemistry-Frozen 1:250, Radioimmunoassay 1:25000 IHC: Staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine).

3 Images Western Blot: Endothelin-1 Antibody (TR.ET.48.5) [NB ] - Analysis of 25 ug of PC12 cell lysates. Page 2 of 5 v.20.1 Updated 2/12/2019 Immunocytochemistry/Immunofluorescence: Endothelin-1 Antibody (TR.ET.48.5) [NB ] - Analysis of Endothelin 1 (green) showing positive staining in the secretion of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) [NB ] - Immunolocalization of ET-1 in human bowel. Analysis of Endothelin 1 in 3T3 cells compared to an isotype control

4 Immunocytochemistry/Immunofluorescence: Endothelin-1 Antibody (TR.ET.48.5) [NB ] - Analysis of Endothelin 1 (green) showing positive staining in the secretion of PC12 cells (right) compared with a negative control in the absence of primary antibody (left). Page 3 of 5 v.20.1 Updated 2/12/2019 Immunocytochemistry/Immunofluorescence: Endothelin-1 Antibody (TR.ET.48.5) [NB ] - Analysis of Endothelin 1 (green) showing positive staining in the secretion of HUVEC cells (right) compared with a negative control in the absence of primary antibody (left). Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) [NB ] - Immunolocalization of ET-1 in human bowel. Analysis of Endothelin 1 in HepG2 cells compared to an isotype control

5 Analysis of Endothelin 1 in 293T cells compared to an isotype control Page 4 of 5 v.20.1 Updated 2/12/2019 Analysis of Endothelin 1 in HepG2 cells compared to an isotype control Publications Mitrakas L, Gravas S, Karasavvidou F et al. Endothelin-1 overexpression: a potential biomarker of unfavorable prognosis in non-metastatic muscle-invasive bladder cancer Tumour Biol Jan 28 [PMID: ] Talati M, West J, Blackwell TR et al. BMPR2 mutation alters the lung macrophage endothelin-1 cascade in a mouse model and patients with heritable pulmonary artery hypertension Am J Physiol Lung Cell Mol Physiol 2010 Sep [PMID: ] (IHC, Human)

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG1 Isotype Control (MG1) NB AF405 Endothelin-1 Antibody (TR.ET.48.5) [Alexa Fluor 405] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: