Product Datasheet. Vanilloid R1/TRPV1 Antibody NB Unit Size: 0.05 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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1 Product Datasheet Vanilloid R1/TRPV1 Antibody NB Unit Size: 0.05 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Publications: 15 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/21/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Vanilloid R1/TRPV1 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer 0.05 ml This product is unpurified. The exact concentration of antibody is not quantifiable. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Unpurified Whole antisera Page 1 of 4 v.20.1 Updated 8/21/2017 Product Description Host Rabbit Gene ID 7442 Gene Symbol Species TRPV1 Human, Rat, Primate Reactivity Notes Human and Primate reactivity reported in scientific literature (PMID: ). Mouse reactivity is reported in scientific literature (PMID: ), but this antibody may not be suitable for detection of the mouse protein in WB. Specificity/Sensitivity Vanilloid Receptor 1 Immunogen Product Application Details Applications Synthetic peptide: RASLDSEESESPPQENSC, corresponding to amino-terminus of Rat VR1, amino acids Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunoprecipitation Recommended Dilutions Western Blot 1:100-1:2000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation, Immunohistochemistry-Paraffin 1:1000, Immunohistochemistry-Frozen Application Notes Immunohistochemistry and Immunocytochemistry 1:1000. Dilution listed only as a recommendation. Optimal dilution should be determined by investigator. IHC- Frozen reactivity reported in scientific literature (PMID: ), Immunoprecipitation reactivity reported in scientific literature (PMID: )

3 Images Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB ] - CGRP and TRPV1 are co-expressed in most cultured trigeminal ganglion neurons. (A and C) CGRP expression in d2 trigeminal ganglion neurons plated on poly-d-lysine-coated coverslips. (B and D) The same culture shown in panel A or C costained for TRPV1 expression. Magnification bar = 200m (A and B) or 40m (C and D) Page 2 of 4 v.20.1 Updated 8/21/2017 Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NB ] - TRPV1 Antibody [NB ] - Dorsal Root Ganglion Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB ] - TRPV1 staining in rat trigeminal ganglion. Image courtesy of Dr. Norman Capra, University of Maryland

4 Publications Huang WX, Yu F, Sanchez RM et al. TRPV1 promotes repetitive febrile seizures by pro-inflammatory cytokines in immature brain. Brain Behav. Immun Mar 20 [PMID: ] (IP, Mouse) Sappington RM, Sidorova T, Ward NJ et al. Activation of Transient Receptor Potential Vanilloid-1 (TRPV1) Influences How Retinal Ganglion Cell Neurons Respond to Pressure-Related Stress Channels (Austin) Feb 25 [PMID: ] (WB, Human, Rat, Primate) Zhao Z, Fan H, Higgins T et al. Fufang Kushen injection inhibits sarcoma growth and tumor-induced hyperalgesia via TRPV1 signaling pathways. Cancer Lett Sep 19 [PMID: ] Starowicz K, Makuch W, Korostynski M et al. Full Inhibition of Spinal FAAH Leads to TRPV1-Mediated Analgesic Effects in Neuropathic Rats and Possible Lipoxygenase-Mediated Remodeling of Anandamide Metabolism PLoS One 2013 [PMID: ] (IHC-Fr, ICC/IF, Rat) Sappington RM Calkins DJ. Contribution of TRPV1 to Microglia-Derived IL-6 NF{kappa}B Translocation with Elevated Hydrostatic Pressure. Invest Ophthalmol Vis Sci;49(7): [PMID: ] Sharif R et al. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nature Neuroscience; (1): [PMID: ] Liapi A et al. Extensive co-localization heteromultimer formation of the vanilloid receptor-like protein TRPV2 the capsaicin receptor TRPV1 in the adult rat cerebral cortex. Eur J Neurosci; 22(4): [PMID: ] Aoki Y et al. Expression Co-Expression of VR1, CGRP, IB4-Binding Glycoprotein in Dorsal Root Ganglion Neurons in Rats: Differences Between the Disc Afferents the Cutaneous Afferents. Spine; 30(13): [PMID: ] 9 Zimov S Yazulla S. Localization of vanilloid receptor 1 (TRPV1/VR1)-like immunoreactivity in goldfish zebrafish retinas: Restriction to photoreceptor synaptic ribbons. J Neurocytol; 33: [PMID: ] Zahner MR, Li DP, Chen SR, Pan HL. Cardiac vanilloid receptor 1-expressing afferent nerves their role in the cardiogenic sympathetic reflex in rats. J Physiol; 551(2): [PMID: ] Nakatsuka T et al. Distinct Roles of P2X Receptors in Modulating Glutamate Release at Different Primary Sensory Synapses in Rat Spinal Cord. J Neurophys; 89: [PMID: ] Labrakakis C et al. Localization function of ATP GABAA receptors expressed by nociceptors other postnatal sensory neurons in rat. J Physiol; 549(1): [PMID: ] More publications at Page 3 of 4 v.20.1 Updated 8/21/2017

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NB PEP HAF008 NB7160 NBP Vanilloid R1/TRPV1 Blocking Peptide Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody [HRP] Rabbit IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: