Product Datasheet. DLL4 Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 14

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1 Product Datasheet DLL4 Antibody NB Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 14 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 3/3/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB DLL4 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity 0.1 mg Please see the vial label for concentration. If unlisted please contact technical services. Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.01% Sodium Azide IgG Immunogen affinity purified Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, ph 7.2 Product Description Host Rabbit Gene ID Gene Symbol Species Reactivity Notes Immunogen Product Application Details Applications DLL4 Human, Mouse, Rat Chimpanzee 91%. Mouse reactivity reported in scientific literature (PMID: ). Rat reactivity reported in scientific literature (PMID: ). Anti-DLL4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DLL4. Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Western Blot 1:500-1:2500, Flow Cytometry, ELISA 1: :50000, Immunohistochemistry 10 ug/ml-20 ug/ml, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry- Paraffin 20 ug/ml Application Notes Page 1 of 4 v.20.1 Updated 3/3/2019 Expect a band approximately 74 kda in size corresponding - DLL4 by western blotting in the appropriate cell lysate or extract.flow Cytometry was reported in scientific literature. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID ). Use in FLOW reported in scientific literature (PMID: ).

3 Images Western Blot: DLL4 Antibody [NB ] - Shows detection of a 74- kda band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-delta-4 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time. Page 2 of 4 v.20.1 Updated 3/3/2019 Immunohistochemistry-Paraffin: DLL4 Antibody [NB ] - This antibody was used at 20ug/mL in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary.

4 Publications Muguruma Yukari, Hozumi Katsuto, Warita Hiroyuki et al. Maintenance of Bone Homeostasis by DLL1-Mediated Notch Signaling. J Cell Physiol 2017 [PMID: ] (IHC, Mouse) Davis RB, Kechele DO, Blakeney ES et al. Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation. JCI Insight. Mar :00AM [PMID: ] (ICC/IF, Human) Kontomanolis E, Mitrakas A, Giatromanolaki A et al. A pilot study on plasma levels of micro-rnas involved in angiogenesis and vascular maturation in patients with breast cancer Med. Oncol Feb :00AM [PMID: ] (IHC, Human) Anjos-Afonso F, Currie E, Palmer HG et al. CD34(-) cells at the apex of the human hematopoietic stem cell hierarchy have distinctive cellular and molecular signatures. Cell Stem Cell Aug 1 [PMID: ] (IHC, Human) Kontomanolis E, Panteliadou M, Giatromanolaki A et al. Delta-like ligand 4 (DLL4) in the plasma and neoplastic tissues from breast cancer patients: correlation with metastasis. Med. Oncol May 01 [PMID: ] (IHC-P, Human) Wu S, Esumi S, Watanabe K et al. Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon. Development 2011 Jun [PMID: ] Indraccolo S, Minuzzo S, Masiero M et al. Cross-talk between tumor and endothelial cells involving the Notch3-Dll4 interaction marks escape from tumor dormancy. Cancer Res 2009 Feb [PMID: ] Zhang Z, Zeng B, Zhang Z et al. Suppressor of cytokine signaling 3 promotes bone marrow cells to differentiate into CD8+ T lymphocytes in lung tissue via up-regulating Notch1 expression. Cancer Res 2009 Feb [PMID: ] Seol JH, Feldman RM, Zachariae W et al. Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential. Leukemia 2004 Apr [PMID: ] Tohda S, Murata-Ohsawa M, Sakano S et al. Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int J Oncol 2003 May [PMID: ] Liu ZJ, Shirakawa T, Li Y et al. Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol Cell Biol 2003 Jan [PMID: ] Gupta S, Li S, Abedin MJ et al. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol : [PMID: ] (WB, Rat) More publications at Page 3 of 4 v.20.1 Updated 3/3/2019

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