Product Datasheet. Caspase-8 Antibody - (active/cleaved) NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles.

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1 Product Datasheet Caspase-8 Antibody - (active/cleaved) NB Unit Size: 0.05 ml Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 38 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 11/18/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Caspase-8 Antibody - (active/cleaved) Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Target Molecular Weight Product Description Host 0.05 ml This product is unpurified. The exact concentration of antibody is not quantifiable. Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Unpurified Whole antisera 55.4 kda Rabbit Gene ID 841 Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen Product Application Details Applications CASP8 Human, Mouse, Rat, Gerbil Possible reactivity with canine species. This is specific for the active (cleaved) Caspase 8 protein, both large (17-21 kda) and small (10-13 kda) subunits. Recombinant catalytically active human Caspase-8 protein. Page 1 of 4 v.20.1 Updated 11/18/2018 Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunoprecipitation Recommended Dilutions Western Blot 1:1000-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen 1:10-1:500

3 Images Western Blot: Caspase-8 Antibody - (active/cleaved) [NB ] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. Image from verified customer review. Page 2 of 4 v.20.1 Updated 11/18/2018 Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB ] - Sections from a brain tissue array stained for Caspase- 8 expression using NB at 1:2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells. Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB ] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB at 1:2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).

4 Publications Baquedano Eva, Burgos-Ramos Emma, Canelles Sandra et al. Increased oxidative stress and apoptosis in the hypothalamus of diabetic male mice in the insulin receptor substrate-2 knockout model. Dis Model Mech 2016 [PMID: ] (ICC/IF, Mouse) Turtle JD, Strain MM, ReYnolds JA et al. Pain Input After Spinal Cord Injury (SCI) Undermines Long-Term Recovery and Engages Signal Pathways That Promote Cell Death. Front Syst Neurosci. Jun :00AM [PMID: ] (WB, Rat) Guclu H, Doganlar ZB, Gurlu VP et al. Effects of cisplatin-5-fluorouracil combination therapy on oxidative stress, DNA damage, mitochondrial apoptosis, and death receptor signalling in retinal pigment epithelium cells. Cutan Ocul Toxicol Apr :00AM [PMID: ] (Human) Philion C, Ma D, Ruvinov I et al. Cymbopogon citratus and Camellia sinensis extracts selectively induce apoptosis in cancer cells and reduce growth of lymphoma xenografts in vivo. Oncotarget 2017 Nov 18 [PMID: ] (IHC-Fr, Human) Li F, Wang T, Wang Z et al. Histone deacetylase inhibitor quisinostat activates caspase signaling and upregulates p53 acetylation to inhibit the proliferation of HepG2 cells Mol Med Rep 2017 Aug 24 [PMID: ] (Human) Liu PF, Hu YC, Kang BH et al. Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma PLoS ONE 2017 Jul 12 [PMID: ] (IHC-P, Human) Xiao J, Yao J, Jia L et al. Protective Effect of Met12, a Small Peptide Inhibitor of Fas, on the Retinal Pigment Epithelium and Photoreceptor After Sodium Iodate Injury. Invest. Ophthalmol. Vis. Sci. Mar :00AM [PMID: ] (WB, Rat) Chi W, Hua X, Chen X et al. Mitochondrial DNA oxidation induces imbalanced activity of NLRP3/NLRP6 inflammasomes by activation of caspase-8 and BRCC36 in dry eye. J. Autoimmun Feb 23 [PMID: ] (ICC/IF, Mouse) Gao Y, Yang H, Fan Y et al. Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis. Mediators of Inflammation. Dec :00AM [PMID: ] (WB, Rat) Garraway SM, Woller SA, Huie JR et al. Peripheral noxious stimulation reduces withdrawal threshold to mechanical stimuli after spinal cord injury: role of tumor necrosis factor alpha and apoptosis. Pain 2014 Nov [PMID: ] (WB) Wu CC, Cheng CH, Lee YH et al. Ursolic Acid Triggers Apoptosis in Human Osteosarcoma Cells via Caspase Activation and the ERk1/2 MAPk Pathway. J. Agric. Food Chem. Jun :00AM [PMID: ] (WB, Human) Magarin M, Pohl T, Lill A et al. Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress. J. Mol. Cell. Cardiol Apr 19 [PMID: ] (IHC-P, Mouse) More publications at Page 3 of 4 v.20.1 Updated 11/18/2018

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NBP HAF008 NB7156 NBP Caspase-8 Knockout HeLa Cell Lysate Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: