Cellular metabolism stimulator
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1 Cellular metabolism stimulator Temporary version
2 Derivative of pantothenic acid (Vitamin B5) to stimulate skin metabolism. D-PTA demonstrates a great penetration in the skin to: Activate energetic pathways......for a repairing and soothing effect. D-PTA Precursor of Coenzyme A ++ Stimulation of energetic pathways (glycolysis, citric acid or even lipid synthesis) Sun, extreme temperature, environmental aggressions, shaving,... D-Panthenyl Triacetate (D-PTA) is a derivative of D-Panthenol (D-PAN) which is well known in the literature for its several benefits on skin: anti-irritant properties, repairing the skin barrier, or also stimulating proteins involved in UV protection. Activation ok skin cells regeneration Wound healing with a better moisture retaining capacity
3 Effect of D-PTA on essential energetic pathways (ex vivo tests) Protocol: D-PTA was tested for its ability to activate several biochemical markers on skin. Twelve explants placed in medium used to support growth were treated, or not, with a cream containing 2% D-PTA. After 6 or 24h of incubation, punches of explants were prepared for mrna extraction. 27 markers of skin metabolism were then analyzed by qrt-pcr. Result: D-PTA significantly activates 25% of the test markers. These are involved in metabolic pathway such as glycolysis, the citric acid circle or lipid synthesis. % vs Control Cream Metabolism markers mrna from human skin explants PTA 6h PTA 24h ACO2 MDH2 HMGCS2 GPI G6PD SULT2B1 ACO2: Aconitase 2 mitochondrial, aconitate hydratase MDH2: Malate dehydrogenase 2, NAD (mitochondrial) Citric acid cycle HMGCS2: 3 hydroxy-3-methylglutaryl-coenzyme A synthase 2 (mitochondrial) Mevalonate pathway GPI: Glucose phosphate isomerase Glycolysis G6PD: Glucose-6-phosphate dehydrogenase SULT2B1: Cholesterol sulfotransferase Lipid synthesis
4 Penetration of the active in the skin (Clinical efficacy) Protocol: D-Panthenol (D-PAN) and D- PTA were tested for their ability to penetrate the skin. Three volunteers applied 3 products on 3 areas of their forearm: a control, a gel containing 3% D-PAN, or a gel with 3% D-PTA. The treated areas were measured by confocal Raman technology (3510 Skin Composition Analyzer) 1, 5 and 24h after application. Result: Comparing this two products, D-PTA offers a quicker and deeper penetration in the skin. PTA concentration (mg/gr keratin) Depth [micron] PAN concentration (mg/gr keratin) PTA content PAN content baseline Appl time 1hr Appl time 5h Depth [micron]
5 Conversion of D-PTA in D-Panthenol in the skin (Clinical efficacy) Protocol: D-PAN and D-PTA were tested for their ability to penetrate the skin. Three volunteers applied 3 products on 3 areas of their forearm: a control, a gel containing 3% D-PAN, or a gel with 3% D-PTA. The treated areas were measured by confocal Raman technology (3510 Skin Composition Analyzer) 1, 5 and 24h after application. PAN concentration (mg/gr keratin) PAN content when PTA applied Result: In 24h, D-PTA is transformed into D-Panthenol in the deeper skin layers, due to its de-acetylation Depth [micron] baseline Appl time 1hr Appl time 5h Measurements have been done only for 24h. It certainly shows only the beginning of the de-acetylation of D-PTA. After 48h a further increase in the conversion of D-PTA in D-PAN might be expected.
6 Stimulation of wound healing (Clinical efficacy) Protocol: A saline treatment, a placebo cream and emulsions containing either 3% D-PAN or 3% D-PTA were tested for their ability to stimulate skin healing. A double-blind clinical trial on 37 volunteers (female and male aged between 40 and 61) was run. Suction blisters were generated by a vacuum of mbar on skin and then immediatly treated. TEWL was measured after 30 min(t0) and 72h. Result: After only 72h, D-PTA at 3% improves skin repair by 8.7%. TEWL Measurement of transepidermal water loss ,7% 35 * T0 72 h *p<0.05 compared to saline, Student s t Test Saline Placebo 3% D-PAN 3% D-PTA
7 TECHNICAL INFORMATION INCI: Origin: Preservation: Appearance: Solubility: Dosage: Processing: Regulatory status: Claims: Panthenyl Triacetate Organic Synthesis Preservative free Clear, nearly colorless, highly viscous liquid Oils, alcohols, glycols soluble 1% - 5% Can be incorporated in any formula in liquid form at ph between 5 and 8 and high or moderate temperature CN, JP, KR*, HK, AU, ASEAN, USA, CA, South-Am, Mid-East, EU Cellular metabolism activator, repairing, soothing * Registration pending SAFETY ASSESSMENT Ocular irritation: Skin irritation: Mutagenicity: BCOP assay - non irritant at 100% Closed epicutan patch test - non irritant at 10% Ames assay - OECD non mutagenic
8 Thank you Induchem AG Industriestrasse 8A CH-8604 Volketswil Induchem USA, Inc. 535 Fifth Avenue, Floor 23 New-York, NY10017 Induchem France SAS 171 Bis Avenue Charles de Gaulle FR Neuilly Sur Seine
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