Foot and Mouth Disease in ASEAN countries and related activities

Transcription

1 9 th International Veterinary Immunology Symposium (IVIS) Tokyo, Japan August 2010, Foot and Mouth Disease in ASEAN countries and related activities Wilai Linchongsubongkoch, DVM OIE Reference Laboratory Regional Reference Laboratory for FMD in South East Asia Department of Livestock Development, Thailand

2 Outline 1. FMD status, 2. Strain characterisation and vaccine matching 3. Molecular epidemiological analysis 4. SEA-C-FMD initiative i i i 5. FMD Reference Laboratory Network activities

3 1. FMD global status in 2009/Jun 2010 During 2009 there were no FMD outbreaks in countries listed by the OIE as FMD-free without vaccination Rep. Korea Columbia Chinese Taipei (Taiwan) FMD Free Endemic Free with vaccination Free. Virus present in game parks Source: WRL, Perbright Laboratory, UK Intermediate, sporadic Countries with multiples zones: FMD free, free with vaccination or not free

4 1.1 Positive samples:2009/jun 2010 Country Jun : 535 isolates recovered from 1037 samples (53.2%) Serotype recovered O A SAT 1 SAT 2 SAT 3 No detection of serotype C since 2004 (Brazil and Kenya) Source: WRL, Perbright Laboratory, UK Iran Bhutan Pakistan Afghanistan Hong Kong South Korea Japan United Arab Emirates Turkey O, A O O O O O, A O O O Senegal SAT 2 Asia 1 Ethiopia O, SAT 2 Kenya O, SAT 1, SAT 2 Nigeria Ecuador O O Total 440 O= 183 SAT1 = 19 A = 71 SAT 2 = 15 NVD = 152

5 1.2 FMD status in Asia (Source: OIE, WAHIS) Type O in Japan 2010 Type O, cattle and pigs Previous occurrence: 05/2000 Start: 20/04/ 2010 Resolved: 5/07/2010 Control measure: - Stamping out - Movement control inside country - Disinfection premise - Emergency Vaccination, no treatment of animals

6 1.2 FMD status in Asia (Source: OIE, WAHIS) Type O in Korea 2010 (Rep of) Type A, in January 2010, Type O in April 2010 Control measure: - stamping out - Movement control in country - no treatment of animal - vaccination prohibited Source: WRL, UK

7 1.2 FMD status in Asia (Source: OIE, WAHIS) FMD in Chinese Taipei in 2010 FMD in China (People s Rep. of) Type O, swine Start : Feb 2010 Type O, swine Control measure: - Quarantine Control measure: no information - Movement control in country - Screening, zoning - Disinfection i i premises - Stamping out No vaccine or treatment - No vaccine or treatment animals

8 1.3 FMD Status of South East Asia Most common : type O and A Philippine : FMD free whole country without and with vaccination Malaysia : Free in Sabah and Salawak island Indonesia : FMD free FMD Free Zone FMD Infected Areas Source : Ronel Abila, OIE-RCU

9 Serotype distribution per year Source: OIR-RCU, Bangkok

10 Yearly outbreaks per country Source: ARAHIS, OIE-RCU, Bangkok

11 Outbreaks by Country (2005- Jun 2010) Cambodia Laos Myanmar Malaysia Philippines Thailand Viet Nam Source: OIR-RCU, Bangkok

12 Conclusion of FMD serotypes in Southeast Asia during Country Cambodia??? O O O, A O, A O, A??? O Indonesia NO OUTBREAK Lao PDR O, A O O A O, A O O Malaysia O, A O, A O O, A O O, A O O Myanmar O O O, Asia 1 O O O O O NO Philippines O O O OUTBREAK Thailand O, A O, A O, A O, A O, A O, A O, A O Viet Nam O O, A O, A Asia 1 O, A, Asia 1 O, A, Asia 1 O, Asia 1 O, A O Reference: SEAFMD Monthly Outbreak Report

13 Type O in 2009: Myanmar Thailand, Cambodia, Vietnam, Malaysia Type O in 2010: Myanmar. Malaysia, Vietnam Source: OIE-RCU, Bangkok

14 Type A in 2009: Thailand, Malaysia, Vietnam Type A in 2010: No outbreak

15 Type Asia1in 2005 : Myanmar and Vietnam Type Asia1in 2007: Vietnam

16 Main factor caused from animal movement, Cattle/Buffalo movement critical points and hotspots

17 Pig and Frozen pork movement

18 2. Strain characterisation and vaccine matching Table1. Vaccine matching Country Year Total sample Poor Matching Range of r-value by LP ELISA moderate matching Good Matching (r-value) Lao of FMDV type O In 2009 Myanmar Vietnam Thailand Total (100%) Source: WRL, UK

19 Country Total sample Range of r-value by LP ELISA test Poor Matching Moderate matching Good Matching Lao Vietnam 6-1* 5 Thailand (6.25% ) 15 (93.75%) *r-value = 0.33, sample from pig Source: WRL, UK

20 20 Source: WRL, UK Antigenic characterisation by vaccine matching (r-value) by VNT and LP ELISA,r-value data from April June 2010

21 3. Molecular epidemiology analysis of FMD in South East Asia Serotype O : 3 topotypes * SEA topotype) - TAI, MYA, MAY. Lao, CAM, VIT * Pan Asia topotype : (MAY, LAO, CAM, VIT, TAI) SEA ME-SA Cathay O/VIT/8/2005(*RRL) O/CAM/1/2004 (*RRL) O/VIT/5/2005(*RRL) O/CAM11/2006(*RRL) / O/CAM15/2006(*RRL) /2006(* O/LAO/30/2003 O/LAO8/2006(*RRL) O/LAO4/2006(*RRL) O/LAO6/2006(*RRL) O/SYR/1/2002 O/VIT/3/2005(*RRL) O/SKR/1/2000 O/LAO/2/2000 O/CAM/4/2000 O/TAI/39/2009 (*RRL) O/TAI/51/2009 (*RRL) O/TAI/50/2009 (*RRL) O/TAI/42/2009 (*RRL) O/TAI/38/2009 (*RRL) O/TAI/36/2009 (*RRL) O/TAI/35-1/2009 (*RRL) O/TAI/56/2009 (*RRL) O/TAI/4/2009 (*RRL) O/TAI/43/2008 (*RRL) O/TAI/11/2009 / / (*RRL) O/TAI/66/2008 /66/2008 (*RRL) O/TAI/27-2/2009 (*RRL) O/TAI/40/2008 (*RRL) O/TAI/41/2008 (*RRL) O/TAI/42/2008 (*RRL) O/TAI/24/2007 (*RRL) O/MYA/1/2008 (*RRL) O/TAI/5/2008 (*RRL) O/TAI/7/2008 (*RRL) O/LAO/18/2007 (*RRL) O/TAI/12-2/2007 (*RRL) O/MYA/2/2007 (*RRL) O/MYA/2/2008 (*RRL) O/LAO/19/2007 (*RRL) O/TAI/17-2/2007 (*RRL) O/TAI/33/2007 (*RRL) O/TAI/25/2007 (*RRL) O/TAI/22/2007 (*RRL) O/TAI/34-2/2007 (*RRL) O/TAI/18/2007 (*RRL) O/TAI/12-4/2007 (*RRL) O/LAO/20/2007 (*RRL) O/MYA/1/2009 (*RRL) O/TAI/44/2008 (*RRL) O/TAI/8-1/2009 (*RRL) O/TAI/15-2/2009 (*RRL) O/TAI/55/2008 (*RRL) O/TAI/9-7/2009 (*RRL) O/TAI/75/2008 (*RRL) O/TAI/45-2/2008 (*RRL) O/TAI/60/2008 (*RRL) O/TAI/14/2009 (*RRL) O/TAI/P6/2009 (*RRL) O/TAI/P25/2009 (*RRL) O/TAI/9-2/2010 (*RRL) O/TAI/53-1/2009 (*RRL) O/TAI/53-2/2009 (*RRL) O/TAI/1-1/2010 (*RRL) O/TAI/1-2/2010 (*RRL) O/TAI/64/2009 (*RRL) O/TAI/62/2009 (*RRL) O/TAI/43/2009 (*RRL) O/TAI/21/2005(*RRL) O/MYA/5/2004(*RRL) O/MYA/4/2006 (*RRL) O/LAO/4/2001 O/VIT18/2006(*RRL) O/VIT16/2006(*RRL) O/VIT20/2006(*RRL) O/VIT19/2006(*RRL) O/MYA/2/2006 (*RRL) O/TAI/14/2005(*RRL) O/TAI/15/2005(*RRL) O/VIT/6/2005(*RRL) O/VIT/9/2005(*RRL) O/MYA/2/2004(*RRL) O/TAI/36/2004(*RRL) O/MYA/1/2004(*RRL) O/MYA/4/2005 (*RRL) O/VIT/4/2005(*RRL) O/VIT/10/2005(*RRL) O/MYA/2/2009 (*RRL) O/MYA/3/2009 (*RRL) O/TAI/4/2010 (*RRL) O/TAI/5/2010 (*RRL) O/TAI/55-1/2009 (*RRL) O/TAI/55-2/2009 (*RRL) O/TAI/55-3/2009 (*RRL) O/MYA/3/2004(*RRL) O/MYA/4/2004(*RRL) O/MYA/7/98 (DQ164925) O/TAI/37-1/2004(*RRL) O/MYA/1/2006 (*RRL) O/TAI/16/2007 (*RRL) O/TAI/4/2006R1B1 (*RRL) O/TAI/70/2006 (*RRL) O/TAI/25/2006 (*RRL) O/TAI/9-1/2007 (*RRL) O/TAI/10/2007 (*RRL) O/TAI/9-2/2007R1 (*RRL) O/LAO/22/2007R1 (*RRL) O/LAO/24/2007 (*RRL) O/LAO/21/2007R1 (*RRL) O/LAO/23/2007 (*RRL) O/LAO/25/2007R1 (*RRL) O/LAO/26/2007R1 (*RRL) O/LAO/1/2008 (*RRL) O/LAO/3/2008 (*RRL) O/LAO/2/2008 (*RRL) O/LAO/4/2008 (*RRL) O/LAO/5/2008 (*RRL) O/TAI/189/87reference strain (*RRL) O/CAM/11/94 (AJ294906) O/CAM/12/94 (AJ294907) O/MAY/6/2003 O/MYA/3/2006 (*RRL) PanAsia strain Mya98strain O/VIT/1/2005(*RRL) O/VIT/7/2005(*RRL) O/VIT/2/2005(*RRL) O/VIT21/2006(*RRL) O/PHI/5/2000 (DQ164947) O/PHI/5/99 O/PHI/2/2004 (DQ164956) O/TAI/54-1/2005B1(*RRL) O/TAI/54-2/2005 (*RRL) O/TAI/53-2/2005(*RRL) O/TAI/64-2/2005 (*RRL) O/TAI/64-1/2005(*RRL) O/VIT/3/2004 (DQ165034) O/VIT/2/2004 (DQ165033) O/TAW/81/97 O/TAW/83/97 O/PHI/13/95 O/VIT/13/2002 (DQ165025) O/VIT/2/99 O/HKN/1/94 (AJ303495) O/HKN/12/2004 O/HKN/2/2003 O/HKN/1/2002 O/HKN/4/2001 Hkn94strain SEA = South East Asia 0.02 % *RRL = Regional Reference Laboratory for Foot and Mouth Disease Virus in South East Asia Others sample code = Submitted to the GenBank databases ( Source: Panithan Thongtha, Rattanee Thongtha, RRL Up date 11/08/2010

22 * Cathay topotype: EURO SA O/HKN/6/83 (AJ294919) O/VIT/1/2006 O/VIT/2/2006 O/VIT/3/2006 O/MAY/8/2005 O/VIT/9/2005 Vietnam & Malaysia 2005 O/VIT/2/05* (TRRL) O/VIT/7/05* (TRRL) O/VIT/1/2005 O/VIT/1/05* (TRRL) O/TAI/54-1/05B1* (TRRL) O/TAI/54-2/05B1* (TRRL) O/TAI/53-2/05* (TRRL) O/TAI/5/ Vietnam O/TAI/35/05B2* (TRRL) - Thailand in Malaysia O/TAI/6/2005 O/TAI/64-1/05* (TRRL) O/TAI/64-2/05* (TRRL) O/VIT/2/2004 (DQ165033) O/VIT/11/2005 O/HKN/1/2008 O/HKN/2/2008 O/HKN/4/2008 O/HKN/4/ Hong Kong Taiwan * since 2007 no Cathay has been found in SEA counttry O/HKN/1/ O/HKN/2/2009 O/HKN/3/2008 O/HKN/3/2007 O/HKN/4/2007 O/HKN/12/2005 O/HKN/3/2006 O/HKN/1/2006 O/HKN/2/2006 O/HKN/4/2006 O/HKN/5/2006 O/HKN/6/2006 O/HKN/1/2007 O/HKN/2/ O/TAW/1/ O-TW (GQ292740) O-TW (GQ292739) O-TW (GQ292737) O-TW (GQ292738) O-TW (GQ292732) O-TW (GQ292733) O-TW (GQ292734) O-TW (GQ292735) O-TW (GQ292728) O-TW (GQ292729) O/Chu-Pei/TAW/97 O/Yunlin/TAW/97 (AF308157) O/TAI/97 (AY593835) O-TW (GQ292726) O/TAW/83/97 O-TW (GQ292727) O/TAW/4/99 (AJ294928) O/Penghu/TAI/99 (AY593833) O-TW (GQ292731) O-TW (GQ292730) O-TW (GQ292736) Vietnam & Thailand 2005 Hong Kong Taiwan

23 FMDV type O VP1 sequence received from Japan 2010 SEA (Mya 98) Source: WRL, UK

24 FMDV type O Type A

25 Phylogenetic tree of FMDV type A field outbreaks in South East Asia A/TAI/34/2008 (*RRL) A/TAI/2/2007R1 (*RRL) A/TAI/5/2007R2B2 (*RRL) A/TAI/22-1/2008 (*RRL) A/TAI/20/2008 (*RRL) A/TAI/22/2009 (*RRL) A/TAI/25/2009 (*RRL) A/TAI/19/2009 (*RRL) A/TAI/62/2008 (*RRL) A/TAI/37/2009 (*RRL) A/TAI/73/2008 (*RRL) 70 A/LAO/7/2006 (EU667458) 64 A/LAO/8/2006 (EU667459) A/TAI/20-3/2009 (*RRL) A/TAI/12/2009 (*RRL) 50 A/LAO/6/2006 (EU667457) A/TAI/78/2008 (*RRL) A/TAI/77/2008 (*RRL) 100 A/CAM 4/08* (TRRL) A/TAI/71-2/2008 (*RRL) A/TAI/70/2008 (*RRL) A/CAM/1/2006 A/TAI/68/2008 (*RRL) A/TAI/67/2008 (*RRL) 76 A/CAM/2/2006 A/TAI/65/2008 (*RRL) A/TAI/63/2008 (*RRL) A/TAI/56/2008 (*RRL) 55 A/TAI/6/2006 A/TAI/53/2008 (*RRL) 73 A/TAI/9/2006 A/TAI/47-2/2008 (*RRL) A/TAI/47-1/2008 (*RRL) A/TAI/7/2006 A/TAI/4/2006 A/TAI/3/2006 A/TAI/10/2006 A/TAI/2/2006 A/TAI/8/2006 A/TAI/11/ A in China,2009 Source: LVRI, China A/MAY/3/2008 A/TAI 16-1/08* (TRRL) A/TAI 20/08* (TRRL) A/MAY/1/2008 A/MAY/3/2007 A/TAI/11/2007 A/TAI 63/08* (TRRL) A/TAI 47-1/08* (TRRL) A/TAI 77/08* (TRRL) A/TAI 62/08* (TRRL) A/TAI 68/08* (TRRL) A/TAI 70/08* (TRRL) A/HuBWH/CHA/2009 A/TAI 67/08* (TRRL) A/TAI 73/08* (TRRL) A/IND17/77(AF136351) A/IND/A22/India/17/77(AF204108) A/IND145/87(AF136348) A/IND/86/86(AF390670) A/TAI/16-1/2008 / / (*RRL) A/TAI/23-1/2008 (*RRL) A/TAI/26/2006(*RRL) A/TAI/32-2/2006(*RRL) A/TAI/22-2/2006(*RRL) A/TAI/56/2006R1 (*RRL) A/TAI/23/2007 (*RRL) A/TAI/7/2007 (*RRL) A/TAI/49/2006 (*RRL) A/TAI/24-2/2006(*RRL) A/TAI/26-2/2007 (*RRL) A/TAI/61/2006 (*RRL) A/TAI/20-2/2007 (*RRL) A/TAI/51-1/2006 (*RRL) A/TAI/38/2006R1(*RRL) A/TAI/9/2006(*RRL) A/TAI/12/2006(*RRL) A/TAI/29-2/2006(*RRL) A/TAI/66/2006 (*RRL) A/TAI/15/2007 (*RRL) A/TAI/26-1/2007 (*RRL) A/TAI/53/2006 (*RRL) A/TAI/67-1/2006 (*RRL) A/TAI/40-2/2006 (*RRL) A/TAI/47/2006R3 (*RRL) A/TAI/67-2/2006 (*RRL) A/TAI/67-3/2006 (*RRL) A/TAI/42/2006 (*RRL) A/TAI/43/2006 (*RRL) A/TAI/50/2006R1 (*RRL) A/TAI/1/2007 (*RRL) A/TAI/54/2006 (*RRL) A/TAI/58/2006 (*RRL) A/TAI/65/2006R2 (*RRL) A/TAI/45/2006 (*RRL) A/TAI/75/2006R2 (*RRL) A/TAI/80/2006R1B1 (*RRL) A/TAI/11/2004(*RRL) A/TAI/28/2004(*RRL) A/TAI/8/2004(*RRL) A/TAI/74/2006R2B1 (*RRL) A/TAI/1/2005 (*RRL) A/TAI/3-3/2005(*RRL) A/TAI/4/2005(*RRL) A/TAI/6/2005(*RRL) A/TAI/48-2/2004(*RRL) A/TAI/45/2004(*RRL) A/TAI/66/2004(*RRL) A/TAI//2-2004(*RRL) A/TAI/4/2004(*RRL) A/TAI/6-3/2004(*RRL) A/TAI/3507/47(*RRL) A/TAI/54/2008 (*RRL) A/LAO/3/2007 (*RRL) A/TAI/6/2007R1B1 (*RRL) A/LAO/1/2007 (*RRL) A/LAO/2/2007 (*RRL) A/LAO/4/2007 (*RRL) A/LAO/6/2007 (*RRL) A/LAO/5/2007 (*RRL) A/LAO/7/2007 (*RRL) A/LAO/8/2007 (*RRL) A/LAO/9/2007 (*RRL) A/LAO/10/2007 (*RRL) A/LAO/12/2007 (*RRL) A/LAO/13/2007 (*RRL) A/LAO/14/2007 (*RRL) A/LAO/15/2007 (*RRL) A/LAO/16/2007 (*RRL) A/LAO/11/2007 (*RRL) A/CAM/4/2008 (*RRL) A/LAO/17/2007 (*RRL) A/TAI/65/2005B2(*RRL) A/TAI/Sakol/reference strain (*RRL) A/TAI/118/87/reference strain (*RRL) A/TAI/132/reference strain (*RRL) A/TAI/14-1/2004(*RRL) A/CHI/XJ/KT/58(AJ131665) / / / A/IND7/88(AF136369) A/IND396/88(AF136359) 0.02 % EURO-SA = Europe-South America, WA-EA = West Africa-East Africa *RRL = Regional Reference Laboratory for Foot and Mouth Disease Virus in South East Asia Others sample code = Submitted to the GenBank databases A/CHI/NM/XZ/64(AJ131664) A/CHI/NM/EL/60(AJ131663) A/GAM/51/98(AF390862) A/SAU/12/86(M38458) A/IND/99/87(AF136371) A/IND99/87(AF136371) A/CAM/dma/112/01(AY254405) A/CAM/dam/112/06(AY254407) A/CAR/15/2000(AY254413) A/CAM/mki/099/01(AY254409) A/CAR/vdi/044/66(AY254439) WA-EA A/KEN/37/84(AF023526) A/IND78/96(AF136370) A/Hol/Holland-A10(M20715) A/Col/76/Cundinamarca-A27(K03341) A/CHI/GS/LX/62(AJ131666) A/Arg/87/Castellanos-A81(U62263) A/Arg/87/Castellanos(AJ306222) ASIA EURO-SA Source: Panithan Thongtha, Rattanee Thongtha, RRL Up date 11/08/2010

26 Phylogenetic tree of FMDV type Asia1 field outbreaks in South East Asia (Underline) Sporadic outbreaks in Myanmar and Vietnam No outbreak in Thailand since 1998 Not seen in Lao PDR, Malaysia and Cambodia since 2000 No outbreak in 2009 and 2010 SEA Gr. VI 0.02 % *RRL = Regional Reference Laboratory for FMD in South East Asia Others sample code = Submitted to the GenBank databases ( Source: Panithan Thongtha, Rattanee Thongtha, RRL Up date 25/02/09 Asia1/HKN/1/2005(DQ121114) Asia1/HKN/2/2005(DQ121115) Asia1/TAJ/1/2004(DQ121402) Asia1/TAJ/2/2004(DQ121403) Asia1/PAK/1/2004(DQ121128) Asia1/PAK/69/2003(DQ121127) Asia1/PAK/30/2002(DQ121124) Asia1/PAK/31/2002(DQ121125) Asia1/IRN/31/2004(DQ121121) Asia1/IRN/10/2004(DQ121119) Asia1/IND/390/97(AF392940) Asia1/IND/278/99(AF392934) Asia1/IND/82/96(AF390705) Asia1/PAK/20/2003(DQ121126) Asia1/IRN/58/99(DQ121122) Asia1/TUR/10/99(DQ121131) Asia1/GRE/2/2002(DQ121113) Asia1/MYA/2/2001(DQ121123) Asia1/IND/673/2003(DQ101240) Asia1/IND/388/2004 (DQ101235) Asia1/IND/139/02(DQ101242) Asia1/BHU/27/2002(DQ121111) Asia1/IND/165/2004(DQ101238) Asia1/VIT/1/2006 (*RRL) Asia1/VIT/2/2006 (*RRL) Asia1/VIT/3/2006 (*RRL) Asia1/VIT/4/2006 (*RRL) Asia1/MYA/1/2005 (*RRL) Asia1/TAI/1/98 (DQ121129) Asia1/85/TAI/reference strain Asia1/IRN/25/2004(DQ121120) Asia1/AFG/4/2001(DQ121110) Asia1/IND/18/80(DQ121116) Asia1/IND/15/81(DQ121117) Asia1/HeB/China/1/2005(EF187274) Asia1/JiangSu/CHA/2005(DQ156527) Asia1/Beijing/China/2005(EF185303) Asia1/QH/China/2005(EF187272) Asia1/Amursky/RUS/2005(DQ121401)

27 4. SEAFMD initiative SEAFMD : South East Asia foot and mouth disease control campaign * First established in 1994, as the OIE Sub-Commission of SEAFMD Control Campaign * Compose of 8 member countries; Malaysia, Thailand, Philippine, Cambodia, Myan mar, Laos, Vietnam and Indonesia * Organized an annual meeting of OIE Sub- Commission since 1 st - 16 th, this year meeting was held in Laos, March 2010 in 2010 : new SEA-C-FMD: * included new member from China as OIE Sub-Commission since May 2010 * approved membership of Brunei and * approved membership of Brunei and Singapore to SEACFMD Sub-Commission

28 2. Program management, resource and funding 1.International Coordination and support 3.Public Awareness and communication 4. Disease surveillance, diagnosis i and reporting and control SEAFMD Campaign 5.Policy, Legislation, Zoning establishment 6.Regional Research and technology transfer 7.Livestock sector development and Private Sector integration 8. Monitoring & Evaluation

29 Goal: SEAFMD 2020 Roadmap To provide the SEAFMD Campaign a long term strategic framework to achieve FMD freedom with vaccination by year 2020 Maintain the OIE recognised free zones without vaccination in Indonesia, Philippines, Malaysia and other areas by 2020 Objective: employ a series of integrated t and harmonised approaches to FMD control In 2010; revision of SEACFMD 2020 Roadmap strengthening on vaccine policy and strategy

30 Establish of zoning: target to achieve FMD freedom with ih vaccination i by year 2020 Free zone Infected zone Buffer zone Control zone Eradication zone 2010 Philippine has recogoniged by OIE as FMD free w/o vaccination in zone 1, 3 * 2011 will free w/o vaccination in zone 2

31 5. FMD Reference Laboratory network activities VRDC Pisanulok VRDC Surin VRDC Lumpang National a level e NIAH BKK RRL Thailand VRDC Choburi VRDC Ratchab uri VRDC Konhke an VRDC Nakonsri Vietnam Cambodia Indonesia OIE-FMD Sub-saharan continent FAO Reference Center Botswana Plum Island USA FAO Rome, Italy OIE-FMD RRL Pakchong Thailand WRL Pirbright UK OIE-FMD Centro Panamericano De Fiebre Aftosa Brasil OIE Laboratorio de ifebre Aftosa de la Directio de Lab Centrol Tecnico Agentina OIE Collabating center for validation, quality assessment Vaccine matching test For vesicular disease Belgium Global level Philippines RRL Thailand Lao PDR Connection of FMD Laboratory Network Malaysia Myanmar Regional level

32 Regional Network : The 5 th SEAFMD Lab Network, Vientiane,, Mar 2010, Outcome: * Improving collection good quality sample by using standard container approved by IATA * Update of diagnostic result and solving Laboratory problems * O i d i t l b t i * Organized interlaboratory comparison program for FMD testing,

33 Global network : 5 th Annual meeting OIE/FAO Reference Laboratory Network meeting, November 2009, Delhi, India Two principle and goals: 1. Understand d global l virus distribution and patterns and making vaccine recommendations 2. Improving the quality of laboratories tests from international and national reference laboratories Require sharing and join evaluation of surveillance information on laboratory diagnoses, serotyping, genetic characterisations and vaccine matching tests and hormonising standards for diagnotis procedures

34

35 Outcome: Global Ref Lab network Update global and regional FMD information Organized the interlaboratory comparison on vaccine matching Proficiency testing (PT) for FMD serology test Recomendation of Regional FMD vaccine strain for each pool Recommendation from WRL for virus strain for antigen bank High priority : O manisa (cover pan asia O1 BFS or Compus A 24 Cruzeiro Asia1 Sharmir A iran-05 SAT 2 Saudi Arabia or equi. Medium priority: A Iran 96 A Iran 87 or equivqlent A Malaysia 97 O Taiwan 97 SAT 2 Zimbabwe b Low priority: A15 Bangkok related strain A Argentina 87 C Noville SAT 2 Kenya, SAT1 Kenya SAT 3 Zimbabwe A Kenya

36 Acknowledgements - Dr. Ronello Abila, Dr. Charie Aviso, OIE-RCU Bangkok - Dr. David Paton, Jef Hammond, Nick Knowles, Dr, Donald King IAH, WRLFMD Perbright, UK - National Institute for Animal Health, Kodaira, Japan - Lanzhou Veterinary Research Institute, (LVRI) P.R. China - National Veterinary Research and Quarantine Service, Rep. of Korea - Dr. Panithan Thongtha, Rattanee Thongtha, RRL, Thailand