OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : :38:48 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: n swine fever Address of laboratory: Ash Road, Pirbright Woking, Surrey, GU24 0NF UNITED KINGDOM Tel.: Fax: address: linda.dixon@pirbright.ac.uk website: Name (including Title) of Head of Laboratory (Responsible Official): Professor John Fazakerley Name (including Title and Position) of OIE Reference Expert: Linda Dixon Which of the following defines your laboratory? Check all that apply: Academic OIE Reference Laboratory Reports Activities,

2 n swine fever - Linda Dixon - united kingdom ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally ELISA (Ab) 11 0 Direct diagnostic tests Nationally Internationally Antigen ELISA 6 Real-time PCR HAD 6 ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2013

3 n swine fever - Linda Dixon - united kingdom Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients ASFV hyperimmune pig sera ELISA provide 0 10ml 1 monoclonal antibody against ASFV protein p30 3ml 1 sera from pigs immunised with attenuated ASFV 2.4ml 1 46 tubes containing plasmids with individual ASFV genes inserted 10ug in 500ul each 1 plasmid - ploxpvp72gus, plasmid pvp72icre 500ul each 1 plasmid pcmvr, plasmid pvsvg, plasmid pdl HTERTpk 1ug in 500ul each 1 ASFV genomic DNA amplified into 30kbp fragments 0.5ug in 500ul 1 ASFV virus isolates BA71V, BEN97/1, GRG2007/1, LIL20/1, LIS60, MALTA78, OURT88/3, OURT88/1, Attenuated Uganda, UGA95/1 virulent Uganda 6ml each isolate 1 OIE Reference Laboratory Reports Activities,

4 4. Did your laboratory produce vaccines? n swine fever - Linda Dixon - united kingdom 5. Did your laboratory supply vaccines to OIE Member Countries? ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses ZAMBIA 09/ Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided KOREA (REP. OF) Advice on diagnostic methods and putting in contact with the Eurpean reference laboratory for ASFV in Spain Visit to Korean Lab and remote assistance CZECH REPUBLIC test selection remote assistance AUSTRALIA Advice on diagnostic methods, pig challenge and selection of ASFV strains for reference remote assistance 4 OIE Reference Laboratory Reports Activities, 2013

5 n swine fever - Linda Dixon - united kingdom ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 1 Carrie Batten, Lorraine Edwards and Chris Oura. Virological diagnosis of n swine fever - comparative study of available tests, 2013.Virus Research, 173, Fishbourne E, Abrams CC, Takamatsu HH and Dixon LK. Modulation of chemokine and chemokine receptor expression following infection of porcine Macrophages with n swine fever virus. Vet Microbiol Mar 23; 162(2-4): doi: /j.vetmic Epub 2012 v 29 Abrams C. C.,Goatley L., Fishbourne E. Chapman D. Cooke L. Oura C. A. Netherton C. L. Takamatsu H-H Dixon L. K. Deletion of virulence associated genes from attenuated n swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus 2013 Virology DOI - doi: /j.virol Dixon L. K., Chapman D. A., Netherton C. L., Upton C 2013 n swine fever virus replication and genomics. Virus Research 173, 3-14 Dixon, L K; Abrams, C C; Chapman, D D G; Goatley, L C; Netherton, C L; Taylor, G; Takamatsu, H H Prospects for development of n Swine Fever virus vaccines 2013 Developments in biologicals 135, DOI: / Fishbourne, E.; Hutet, E.; Abrams, C.; Cariolet, R.; Potier, M. F. le; Takamatsu, H. H.; Dixon, L. K. Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence n swine fever virus isolates Veterinary Research, in press 44 ISSN: Lithgow P., Takamatsu H., Werling D., Dixon L., Chapman D. Correlation of cell surface marker expression with n swine fever virus infection. Accepted Veterinary Microbiology b) International conferences: 2 Oral Presentation and Participation in Discussion. USDA National Veterinary Stockpile Gap Analysis Workshop, Plum Island, USA April 3rd and 4th. Global Alliance for n Swine Fever Virus Research (GARA) Launch, April 5th Plum Island USA OIE Reference Laboratory Reports Activities,

6 n swine fever - Linda Dixon - united kingdom 2nd July ASFV Symposium Friedrich Loeffler Institute, Insel Reims, Germany Oral Presentation. Mini-symposium n swine fever virus, Presentation University of Utrecht n swine fever virus host interactions and vaccine development. Linda Dixon Korean Society of Veterinary Science October 10th n swine fever virus host interactions and vaccine development Linda Dixon Seminar QIA Institute Seoul S. Korea October 8th 2013 n swine fever virus host interactions and vaccine development Linda Dixon Epizootic Disease Control Group Belfast N. Ireland 6th vember The Advance of n swine fever virus. Ana Reis Oral Presentations at EU FP7 ASFORCE Project 1st Annual Meeting Sardinia 21st to 23rd October WP13 Construction of ASFV deletion mutants for testing as candidate vaccine strains WP14 Development of a blocking qrtpcr assay to measure IFN mrna levels WP15 Immunisation of pigs with an attenuated ASFV strain and challenge with parental virulent virus c) National conferences: 3 Poster Presentation National Institute of Biosciences Conference, Roslin Institute, Edinburgh June 18th to 20th. EPIC Conference Edinburgh 22nd and 23 rd Sep d) Other: (Provide website address or link to appropriate information) 4 ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 0 c) Hands-on training courses: 1 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country c) Republic of Korea 1 d) China 1 ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? 6 OIE Reference Laboratory Reports Activities, 2013

7 n swine fever - Linda Dixon - united kingdom Quality management system adopted ISO/IEC Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited ASFV real time PCR (King et al) ASFV real time PCR (Fernandez et al) ASFV ELISA (Ab) ASFV ELISA (Ag) Accreditation body UKAS UKAS UKAS UKAS 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) ToR: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? OIE Reference Laboratory Reports Activities,

8 n swine fever - Linda Dixon - united kingdom 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Diagnostics: real time PCR, ELISA (Ab and Ag), HAD and IPT participant unknown organising OIE ref lab - Algete Spain 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories EU-FP7 ASFORCE Project Coordinated research effort on n swine fever virus Collaboration on development of vaccines, novel diagnostic methods and models for disease spread Community Reference lab. CISA/INIA, Madrid, Spain ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries RAPFIELD PT: evaluation of the LFD assays for the detection of antibodies againts CSFV and ASFV 13 8 OIE Reference Laboratory Reports Activities, 2013

9 n swine fever - Linda Dixon - united kingdom ToR: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,