C57BL/ g/ kg d 14 Th1 Th2

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1 Th/Th C57BL/6 Th/Th2 8 C57BL/ DNFB C57BL/ g/ kg d 4 Th Th2 Th2 Th/Th2 P < 0.0 Th2 Th/Th2 P < 0.05 P < 0.0 C57BL/6 Th2 Th/Th2 Th2 Th/Th2 Th R285.5 A DOI /j.cnki.jncm Effect of Peitu Qingxin Granules on Th/Th2 Cells in Spleen of Atopic Dermatitis Mice Mode LIN Ying MENG Weiwei ZHANG Xian YAN Fenggen LIU Junfeng MO Xiumei XUE Suqin CHEN Dacan Abstract Objective To discuss the effect of Peitu Qingxin granules on Th/Th2 cells in spleen of C57BL/6 mice model of atopic dermatitis induced by hapten. Methods Eighteen male C57BL/6 mice were divided into the blank group the model group and Peitu Qingxin granules group randomly 6 mice in each group. In the model group and Peitu Qingxin granules group C57BL/6 mice were induced by 2 4- dinitrofluorobenzene(dnfb) to establish the model of atopic dermatitis. From the first day of model being established Peitu Qingxin granules group was given 2.47 g/ (kg d) of Peitu Qingxin granules for gavage and the blank group and the model group were given distilled water of the equivalent volume by gavage. Both groups were treated for 4 days continuously. Observed skin lesions and pathological sections of skin tissue of mice determined the degree of ear swelling and detected the expression of Th/Th2 cells in the spleens of mice by flow cytometry. Results Comparing with those in the blank group the thickness of right ears and Th2 cells in the model group were increased obviously while Th/Th2 in the model group was decreased significantly. The thickness of right ears in Peitu Qingxin granules group was raised evidently(p<0.0). In Peitu Qingxin granules group the thickness of right ears and Th2 cells were declined while Th/Th2 was increased significantly when compared with those in the model group(p<0.05 P<0.0). Skin of mice in YN205MS @63.com

2 the blank group was normal. In the model group thickness roughness erythema desquamation erosion and scab were found on the back skin of mice and the pathological manifestations of skin tissues were parakeratosis hyperkeratosis spongiotic edema and acanthosis as well as dermis with massive lymphocytic infiltration. In Peitu Qingxin granules group slight thickness roughness slight erythema mile desquamation and scab were found on the back skin of mice and the degree of inflammation was less than that in the model group. The pathological manifestations of skin tissues in Peitu Qingxin granules group were parakeratosis hyperkeratosis spongiotic edema and slight acanthosis as well as dermis with little lymphocytic infiltration. Conclusion The C57BL/6 mice model of atopic dermatitis exists the imbalance of Th/Th2 mainly with the increase of Th2 cells. Peitu Qingxin granules can reduce the expression of Th2 cells in spleen of the mice model which indicates that regulating the imbalance of Th/Th2 may be the possible mechanism of the anti- inflammatory function of Peitu Qingxin granules. Keywords Atopic dermatitis Peitu Qingxin granules Th cells Animal experiment Mouse (atopic dermatitis AD) g/ ) 2 4- (DNFB)(sigma D529) (BD ) (BD ) CD4 (BD ) IL- 4 (BD ) IL- 4 (BD ) IFN- γ (BD [] ) IFN- γ (BD ) RPMI- 640 [2].3 8 C57BL/6 [3] 6 HEO JC [8] AD 24 [4] CD5 + B Breg 2 cm 2 cm [5] 50 μl ( IL- 2 =4 ) 0.5% DNFB 5 TNF- α [6~7] 20 μl 50 μl 0.2% DNFB C57BL/6 AD Th/Th2 3 ( ) ( =4 Th Th2 ) [9]. 6~8 SPF C57BL/ g/kg 0.25 g/ml ~22 g SPF (25±2) 4 50% ~60% 2 h 24 h.5.4 (2 g/d).2 (BD FACSAriaII).6 Countstar Himac CF5D 3 Allergra x- 22R (.7 3 SATA072) (Eppendorf) ( 0%

3 HE (P<0.0).8 5 ml 37 5 min 2 0% /ml 24 2 μl 37 5% CO 2 5 h 3 ml PBS 2 FITC- CD4 30 min 4 ml μl 4 20 min 5 ml 2 APC- IL- 4 PerCP- Cy5- IFN- γ IL- 4 IFN- γ 4 30 min μl PBS FITC- CD4 CD4 + T CD4 + T Th(CD4 + IFN- γ + ) Th2(CD4 + IL- 4 + ).9 SPSS (x±s) t Mann- Whitney U 2 2. (P<0.0) A. B. C. 2 (x±s) n 6 6 (mm) 0.23±0.0.97± ± P < 0.0 2P < A. B. C Th Th Th2 Th/Th2 (P<0.0)

4 Th2 Th/Th2 (P<0.05 P<0.0) 3 Th2 (P<0.0) Th/Th2 (P<0.05) CD4 + T (Th Th2 Th9 Th7 Th22 Treg) C57BL/6 Th/Th2 Th2 Th/Th2 [0] Th2 Th2 Th/Th2 T IgE IL- 0 Th0 Th2 AD Th2 IL- 4 IL- 3 B IgE []. [M]. IL [~2] IFN- γ Th [2] Liu J Mo X Wu D et al.efficacy of a Chinese 2 4- herbal medicine for the treatment of atopic dermatitis a randomised controlled study [J].Complement C57BL/6 AD IgE IL- 4 Ther Med (5) [3] Th2 [3]. [J] (6) AD [4]. Th Th2 [J] (7) Th Th (P<0.0) Th/Th2 [5]. (P<0.0) [J] Th/Th2 Th2 AD (5) Th/Th2 Th2 [6]. 2 Th Th2 (x±s) n Th Th2 Th /Th2 [4] ± ± ± Th/Th ± ± ± ± ± ±.35 C57BL/6 Th Th2 P < P < 0.0 Th2 Th [D] [7]. IL- 2/TNF- α [D] [8] Heo JC Son HU Kim SL et al.a derivative of L- allo threonine alleviates 2 4- dinitrofluorobenzeneinduced atopic dermatitis indications [J].Biosci Biotechnol Biochem () [9].

5 [J]. A role for Th and Th2 cells in the immunopathogenesis of atopic dermatitis [J].Immunol Today (9) [0] Auriemma M Vianale G Amerio P et al.cytokine (8) and T cells in atopic dermatitis [J].Eur Cytokine Netw [3]. C57BL/ () [J] (5) [] Ong PY Leung DY.Immune dysregulation in atopic dermatitis [J].Curr Allergy Asthma Rep (5) [2] Grewe M Bruijnzeel- Koomen CA Sch 觟 pf E et al [4]. [J] () Rg P < 0.05 Re P < 0.05 RSD P < 0.05 P > % 85.0% P > 0.05 R27.43 A DOI /j.cnki.jncm Quality Identification and Effect Comparative Analysis of Cuiru Fang Chinese Herbal Pieces and Dispensing Granule in Treating Puerperal Insufficient Lactation JIN Qingxia Abstract Objective To identify quality of Cuiru Fang herbal pieces and dispensing granule and analysis and compare their clinical effect in treating puerperal insufficient lactation. Methods Selected three batches of herbal pieces and dispensing granule for quality analysis respectively. Selected 40 patients with puerperal insufficient lactation and divided them into dispensing granule group and Chinese herbal pieces group 20 cases in each. The two groups were given dispensing granule liquids or water decoction of Chinese herbal pieces respectively and clinical effect of the two groups were observed. Results Result of quality analysis suggested that comparing with water decoction of Chinese herbal pieces color of dispensing