beta Amyloid Polyclonal Antibody Catalog Number Product data sheet

Transcription

1 Website: thermofisher.com Customer Service (US): ext. 1 Technical Support (US): ext. 441 beta Amyloid Polyclonal Antibody Catalog Number Product data sheet Details Size 100 µg Host/Isotope Class Type Immunogen Conjugate Form Concentration Purification Rabbit / IgG Polyclonal Antibody A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) beta-amyloid peptide Unconjugated Liquid 0.25 mg/ml Affinity chromatography Storage buffer PBS, ph 7.4 Contains Storage Conditions 0.1% sodium azide -20 C Species Reactivity Species reactivity Published species Human Tested Applications Dilution * ELISA (ELISA) Immunohistochemistry (IHC) Immunoprecipitation (IP) Western Blot (WB) Published Applications Immunohistochemistry (IHC) Immunohistochemistry (Frozen) (IHC (F)) Miscellaneous PubMed (MISC) ELISA (ELISA) Immunohistochemistry (Paraffin) (IHC (P)) Immunohistochemistry - Free Floating (IHC (Free)) Western Blot (WB) Immunoprecipitation (IP) Mouse, Human, Not Applicable See 10 publications below See 3 publications below See 3 publications below See 2 publications below See 2 publications below See 2 publications below See 7 publications below See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Background/Target Information Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gammasecretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer's disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down's syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

2 PubMed References For beta Amyloid Polyclonal Antibody 10 Immunohistochemistry References was used in immunohistochemistry to investigate hypercholesterolemia at various stages of Alzheimer's disease Mouse / 1:200 Not Applicable / 1:500 Mouse / 1:300 Human / 1:100 Mouse / 1:300 Mouse / 1:400 Molecular and cellular neurosciences (Nov 2014; 63: 83) "Vascular pathology of 20-month-old hypercholesterolemia mice in comparison to triple-transgenic and APPSwDI Alzheimer's disease mouse models." Author(s):Hohsfield LA,Daschil N,Orädd G,Strömberg I,Humpel C PubMed Article URL: was used in immunohistochemistry to determine the expression pattern of connexin43 and 30 in two murine models of Alzheimer disease Neuroscience (Nov 2010; 171: 92) "Astroglial connexin immunoreactivity is specifically altered at -amyloid plaques in -amyloid precursor protein /presenilin1 mice." Author(s):Mei X,Ezan P,Giaume C,Koulakoff A PubMed Article URL: was used in immunohistochemistry to examine how changes in testosterone and luteinizing hormone contribute to Alzheimer's disease. Brain research (Jul 2012; 1466: 137) "Evaluation of the effects of testosterone and luteinizing hormone on regulation of -amyloid in male 3xTg-AD mice." Author(s):Rosario ER,Carroll JC,Pike CJ PubMed Article URL: was used in immunohistochemistry to test if D-Ala2GIP protects mice with advanced staged Alzheimer's disease. Journal of Alzheimer's disease : JAD (Oct 2013; 35: 267) "D-Ala2GIP facilitated synaptic plasticity and reduces plaque load in aged wild type mice and in an Alzheimer's disease mouse model." Author(s):Faivre E,Hölscher C PubMed Article URL: was used in immunohistochemistry to investigate dysregulated insulin signaling in Alzheimer's disease and diabetes. The Journal of clinical investigation (Apr 2012; 122: 1339) "An anti-diabetes agent protects the mouse brain from defective insulin signaling caused by Alzheimer's diseaseassociated A oligomers." Author(s):Bomfim TR,Forny-Germano L,Sathler LB,Brito-Moreira J,Houzel JC,Decker H,Silverman MA,Kazi H,Melo HM, McClean PL,Holscher C,Arnold SE,Talbot K,Klein WL,Munoz DP,Ferreira ST,De Felice FG PubMed Article URL: was used in immunohistochemistry to determine the distribution pattern, cellular source, and activity of tissue transglutaminase in cerebral amyloid angiopathy, Alzheimer's disease, and hereditary cerebral hemorrhage with amyloidosis of the Dutch type cases. Neurobiology of aging (Apr 2013; 34: 1159) "Tissue transglutaminase colocalizes with extracellular matrix proteins in cerebral amyloid angiopathy." Author(s):de Jager M,van der Wildt B,Schul E,Bol JG,van Duinen SG,Drukarch B,Wilhelmus MM PubMed Article URL: was used in immunohistochemistry to assess the use of selective estrogen receptor modulators using a murine model of Alzheimer's disease. Endocrinology (May 2008; 149: 2607) "Selective estrogen receptor modulators differentially regulate Alzheimer-like changes in female 3xTg-AD mice." Author(s):Carroll JC,Pike CJ PubMed Article URL: was used in immunohistochemistry to investigate butyrylcholinesterase activity in a mouse model of Alzheimer's disease. Neuroscience (Jul 2015; 298: 424) "Butyrylcholinesterase-knockout reduces brain deposition of fibrillar -amyloid in an Alzheimer mouse model." Author(s):Reid GA,Darvesh S PubMed Article URL:

3 was used in immunohistochemistry to assess the effects of depleting both circulating and brain estrogen levels in a mouse model of Alzheimer's disease. Mouse / 1:300 Neurobiology of disease (Jan 2012; 45: 479) "Effects of aromatase inhibition versus gonadectomy on hippocampal complex amyloid pathology in triple transgenic mice." Author(s):Overk CR,Lu PY,Wang YT,Choi J,Shaw JW,Thatcher GR,Mufson EJ PubMed Article URL: was used in immunohistochemistry to examine the individual and combined effects of estrogen and progesterone on beta-amyloid accumulation, tau hyperphosphorylation, and hippocampal-dependent behavioral impairments Not Applicable / 1:300 3 Immunohistochemistry (Frozen) References The Journal of neuroscience : the official journal of the Society for Neuroscience (Nov 2007; 27: 13357) "Progesterone and estrogen regulate Alzheimer-like neuropathology in female 3xTg-AD mice." Author(s):Carroll JC,Rosario ER,Chang L,Stanczyk FZ,Oddo S,LaFerla FM,Pike CJ PubMed Article URL: was used in immunohistochemistry - frozen section to test if Liraglutide has restorative effects in late-stage Alzheimer's disease in mice. Mouse / 1:500 Neuropharmacology (Jan 2014; 76 Pt A: 57) "Liraglutide can reverse memory impairment, synaptic loss and reduce plaque load in aged APP/PS1 mice, a model of Alzheimer's disease." Author(s):McClean PL,Hölscher C PubMed Article URL: was used in immunohistochemistry - frozen section to study the role of soluble mutant amyloid precursor protein on cognitive function and synaptic structure The Journal of neuroscience : the official journal of the Society for Neuroscience (Jun 2014; 34: 7871) "Genetic modulation of soluble A rescues cognitive and synaptic impairment in a mouse model of Alzheimer's disease." Author(s):Fowler SW,Chiang AC,Savjani RR,Larson ME,Sherman MA,Schuler DR,Cirrito JR,Lesné SE,Jankowsky JL PubMed Article URL: was used in immunohistochemistry - frozen section to examine the effects of peripherally injected liraglutide in an Alzheimer mouse model. 3 Miscellaneous PubMed References The Journal of neuroscience : the official journal of the Society for Neuroscience (Apr 2011; 31: 6587) "The diabetes drug liraglutide prevents degenerative processes in a mouse model of Alzheimer's disease." Author(s):McClean PL,Parthsarathy V,Faivre E,Hölscher C PubMed Article URL: was used in immunohistochemistry to demonstrate that liraglutide has prophylactic properties in Alzheimer's disease models. Behavioural brain research (Oct 2015; 293: 96) "Prophylactic liraglutide treatment prevents amyloid plaque deposition, chronic inflammation and memory impairment in APP/PS1 mice." Author(s):McClean PL,Jalewa J,Hölscher C PubMed Article URL: was used in immunohistochemistry - frozen section to examine the effects of Val(8)GLP-1 in a mouse model of Alzheimer's disease Neurobiology of aging (Feb 2012; 33: 265) "Val(8)GLP-1 rescues synaptic plasticity and reduces dense core plaques in APP/PS1 mice." Author(s):Gengler S,McClean PL,McCurtin R,Gault VA,Hölscher C PubMed Article URL:

4 was used in immunohistochemistry (frozen) to demonstrate that GLP-1 receptor agonists are therapeutic for Alzheimer's disease. 2 ELISA References Mouse / 1:500 Neuropharmacology (Nov 2014; 86: 241) "Lixisenatide, a drug developed to treat type 2 diabetes, shows neuroprotective effects in a mouse model of Alzheimer's disease." Author(s):McClean PL,Hölscher C PubMed Article URL: was used in ELISA to study cholinergic dysfunction in a murine model of Alzheimer's disease. Behavioural brain research (Mar 2013; 240: 146) "Cholinergic degeneration is associated with increased plaque deposition and cognitive impairment in APPswe /PS1dE9 mice." Author(s):Laursen B,Mørk A,Plath N,Kristiansen U,Bastlund JF PubMed Article URL: was used in ELISA to determine the link between lower levels of serum anti-amyloid peptide antibodies and patients with Alzheimer disease when compared to healthy elderly individuals 2 Immunohistochemistry (Paraffin) References Experimental gerontology (Jul 2002; 37: 943) "Patients with Alzheimer disease have lower levels of serum anti-amyloid peptide antibodies than healthy elderly individuals." Author(s):Weksler ME,Relkin N,Turkenich R,LaRusse S,Zhou L,Szabo P was used in immunohistochemistry - paraffin section to characterize transgenic mice that overexpress the signal plus 99-amino acid carboxyl-terminal sequences of beta protein precursor Not Applicable / 1:200 The American journal of pathology (Dec 1998; 153: 1687) "Amyloid-beta deposition in skeletal muscle of transgenic mice: possible model of inclusion body myopathy." Author(s):Fukuchi K,Pham D,Hart M,Li L,Lindsey JR was used in immunohistochemistry - paraffin section to describe patients with Abeta-related angiitis Not Applicable / 1:200 2 Immunohistochemistry - Free Floating References Brain : a journal of neurology (Mar 2005; 128: 500) "Abeta-related angiitis: primary angiitis of the central nervous system associated with cerebral amyloid angiopathy." Author(s):Scolding NJ,Joseph F,Kirby PA,Mazanti I,Gray F,Mikol J,Ellison D,Hilton DA,Williams TL,MacKenzie JM,Xuereb JH, Love S PubMed Article URL: was used in immunohistochemistry - free floating to study the relationship between androgen depletion and Alzheimer's disease Not Applicable / 1:300 The Journal of neuroscience : the official journal of the Society for Neuroscience (Dec 2006; 26: 13384) "Androgens regulate the development of neuropathology in a triple transgenic mouse model of Alzheimer's disease." Author(s):Rosario ER,Carroll JC,Oddo S,LaFerla FM,Pike CJ PubMed Article URL: was used in immunohistochemistry - free floating to examine how ageing affects neurogenesis and oxidative stress in a mouse model of Alzheimer's disease. Mouse / 1:200 7 Western Blot References Brain research (Apr 2012; 1449: 83) "The effect of ageing on neurogenesis and oxidative stress in the APP(swe)/PS1(deltaE9) mouse model of Alzheimer's disease." Author(s):Hamilton A,Holscher C PubMed Article URL:

5 was used in western blot to discuss proteins that regulate the metabolism of the amyloid precursor protein and betaamyloid peptides Not Applicable / 1:4000 Human / 0.2 ug/ml Not Applicable / 5 µg/ml Mouse / 1:500 PloS one (Sep 2008; 3: null) "Receptor-associated protein (RAP) plays a central role in modulating Abeta deposition in APP/PS1 transgenic mice." Author(s):Xu G,Karch C,Li N,Lin N,Fromholt D,Gonzales V,Borchelt DR PubMed Article URL: was used in western blot to study the mediation of the interaction with beta-amyloid peptide and growth factors due to distinct binding sites in the structure of alpha 2-macroglobulin The Journal of biological chemistry (Apr 2002; 277: 13338) "Distinct binding sites in the structure of alpha 2-macroglobulin mediate the interaction with beta-amyloid peptide and growth factors." Author(s):Mettenburg JM,Webb DJ,Gonias SL PubMed Article URL: was used in western blot to elucidate the mechanism of action of 22R-hydroxycholesterol on Abeta-mediated toxicity. Journal of neurochemistry (Dec 2002; 83: 1110) "22R-Hydroxycholesterol protects neuronal cells from beta-amyloid-induced cytotoxicity by binding to beta-amyloid peptide." Author(s):Yao ZX,Brown RC,Teper G,Greeson J,Papadopoulos V was used in immunohistochemistry and western blot to investigate the role of transglutaminases in sporadic inclusion body myositis The Journal of biological chemistry (Mar 2000; 275: 8703) "Sporadic inclusion body myositis correlates with increased expression and cross-linking by transglutaminases 1 and 2." Author(s):Choi YC,Park GT,Kim TS,Sunwoo IN,Steinert PM,Kim SY was used in western blot to test if gradual mitochondrial dysfunction contributes to the initiation and progression of sporadic Alzheimer's disease Journal of bioenergetics and biomembranes (Aug 2005; 37: 207) "Gradual alteration of mitochondrial structure and function by beta-amyloids: importance of membrane viscosity changes, energy deprivation, reactive oxygen species production, and cytochrome c release." Author(s):Aleardi AM,Benard G,Augereau O,Malgat M,Talbot JC,Mazat JP,Letellier T,Dachary-Prigent J,Solaini GC,Rossignol R PubMed Article URL: was used in western blot to develop a novel method for the rapid detection of beta-amyloid aggregates in Alzheimer's patients and transgenic mouse models. Alzheimer disease and associated disorders (Dec 2002; 16: 191) "Rapid detection of protein aggregates in the brains of Alzheimer patients and transgenic mouse models of amyloidosis." Author(s):Xu G,Gonzales V,Borchelt DR was used in western blot to investigate the processing and stability of the glutamate receptor 1 Immunoprecipitation References The Journal of biological chemistry (Jun 2003; 278: 23786) "Glutamate receptor subunit 3 is modified by site-specific limited proteolysis including cleavage by gammasecretase." Author(s):Meyer EL,Strutz N,Gahring LC,Rogers SW PubMed Article URL:

6 was used in immunohistochemistry - paraffin section, immunoprecipitation, and western blot to explore the amyloidogenic potential of mouse A beta The Journal of biological chemistry (Aug 2007; 282: 22707) "Rodent A beta modulates the solubility and distribution of amyloid deposits in transgenic mice." Author(s):Jankowsky JL,Younkin LH,Gonzales V,Fadale DJ,Slunt HH,Lester HA,Younkin SG,Borchelt DR PubMed Article URL: