SP GPI Found in exosomes

Transcription

1 Accession /Name/Functional group Spectr. counts SP GPI Found in exosomes Description Metabolism and energy production (21) CNAG_06770 fructose-bisphosphate aldolase gluconeogenesis and glycolysis CNAG_04659 pyruvate decarboxylase converts pyruvic acid to acetaldehyde; forms catalytically active filaments in filamentous ascomycetes CNAG_05750 ATPase α subunit, mitochondrial ATP synthesis CNAG_06313 phosphoglucomutase glycolysis and gluconeogenesis CNAG_06923 phosphoketolase pentose phosphate pathway CNAG_05918 F0F1 ATP synthase subunit β ATP synthesis CNAG_00441 IMP dehydrogenase inosine 5'-monophosphate dehydrogenase, GTP biosynthesis CNAG_03358 phosphoglycerate kinase key enzyme in glycolysis and gluconeogenesis CNAG_03196 orotate phosphoribosyltransferase pyrimidine biosynthesis CNAG_06101 eukaryotic ADP/ATP carrier mitochondrial ATP:ADP antiporter CNAG_03072 phosphopyruvate hydratase glycolysis CNAG_06694 transthyretin family protein purine metabolism CNAG_06699 glyceraldehyde-3-phosphate glycolysis dehydrogenase CNAG_07445 transketolase pentose phosphate pathway CNAG_07561 phosphogluconate dehydrogenase pentose phosphate pathway CNAG_01984 transaldolase pentose phosphate pathway CNAG_04640 acly protein ATP-citrate synthase, citric acid cycle CNAG_ methyltetrahydropteroyltriglutamatehomocysteine S-methyltransferase similar to ScMet6; amino acid metabolism, cobalamin-independent methionine synthase CNAG_03266 malate dehydrogenase citric acid cycle CNAG_01577 NADP-dependent glutamate dehydrogenase synthesis of glutamate

2 CNAG_03916 glucose-6-phosphate isomerase catalyzes conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis RNA processing and translation (18) CNAG_00417 elongation factor 1-γ mrna translation CNAG_ S ribosomal protein L ribosomal protein CNAG_06811 RPL ribosomal protein CNAG_04883 ribosomal protein S ribosomal protein CNAG_ S ribosomal protein S ribosomal protein CNAG_03577 L10e protein ribosomal protein CNAG_ S ribosomal protein L ribosomal protein CNAG_00370 ubiquitin-carboxy extension protein fusion ribosomal-ubiquitin fusion protein Ubi1, ribosome assembly CNAG_06125 translation elongation factor 1α functions in the binding reaction of aminoacyl-trna (AA-tRNA) to ribosomes CNAG_ s ribosomal protein L ribosomal protein CNAG_05555 ribosomal protein L ribosomal protein CNAG_04050 pre-mrna splicing factor mrna processing CNAG_00933 conserved hypothetical protein probable trna splicing protein, cyclic phosphodiesterase CNAG_05831 Brt endoribonuclease active on mrna CNAG_03249 conserved hypothetical protein RRM domain-containing protein; RNA annealing factor Mlo3 involved in mrna export CNAG_ S ribosomal protein S ribosomal protein CNAG_04799 ribosomal protein L ribosomal protein CNAG_05232 ribosomal protein L ribosomal protein Cell wall-associated/modifying enzymes (14) CNAG_03120 α-1,3-glucan synthase cell wall biosynthesis CNAG_ ,3-β-glucanosyltransferase cell wall biosynthesis and morphogenesis; homolog of S. cerevisiae Gas1, a GPI anchored protein found

3 in lipid rafts CNAG_06085 secreted phospholipase B Plb virulence-related phospholipase CNAG_03465 laccase melanin biosynthesis CNAG_02944 acid phosphatase Aph secreted and vacuolar enzyme implicated in adhesion to the host, regulated by phosphate availability CNAG_05138 exo-β-1,3-glucanase cell wall biosynthesis and modification CNAG_06835 glucosidase Kre β-glucan synthesis-associated protein Kre61 CNAG_01239 chitin deacetylase Cda production of chitosan; essential for cell wall integrity, cell separation and regulation of capsule size CNAG_01230 chitin deacetylase Cda production of chitosan; essential for cell wall integrity, cell separation and regulation of capsule size CNAG_05799 chitin deacetylase Cda converting chitin to chitosan, a cell wall polymer established as a virulence factor for C. neoformans infection of mammalian hosts CNAG_00799 Glycoside hydrolase family cell wall homeostasis CNAG_02225 glycoside hydrolase family cell wall homeostasis CNAG_00914 glucosidase Kre β-glucan synthesis-associated protein Kre6; involved in β-1,6-glucan synthesis, maintenance of cell wall integrity, retention of mannoproteins and capsule production CNAG_06336 glucan 1,3-β-glucosidase protein, homology to Bgl cell wall biosynthesis and remodelling; similar to Candida Mp65 Miscellaneous extracellular enzymes (13) CNAG_04869 carboxylesterase carboxylesterase CNAG_02958 ferroxidase Cfo potentially involved in iron acquisition; oxygen oxidoreductase that catalyzes the oxidization of iron II to iron III CNAG_05731 glyoxal oxidase hydrogen peroxide producing enzyme, regulates cell morphology and virulence in Ustilago maydis

4 CNAG_00150 peptidase Subtilase family protease CNAG_06081 glucose oxidase GMC oxidoreductase CNAG_00407 glyoxal oxidase hydrogen peroxide producing enzyme, regulates cell morphology and virulence in Ustilago maydis CNAG_02030 glyoxal oxidase hydrogen peroxide producing enzyme, regulates cell morphology and virulence in Ustilago maydis CNAG_02189 α-amylase hydrolysis of starch CNAG_04625 serine-type endopeptidase protein hydrolysis CNAG_00919 carboxypeptidase D protein hydrolysis CNAG_00581 endopeptidase aspartic protease CNAG_04291 glycosyl-hydrolase type 1 glutamine amidotransferase (GATase1) domain protein; trehalose utilization CNAG_04373 alginate lyase polysaccharide lyase Capsule production (4) CNAG_04753 lactonohydrolase lactone-hydrolyzing enzyme CNAG_03322 UDP-xylose synthase UXS necessary for capsule xylosylation and virulence CNAG_04969 UDP-glucose dehydrogenase capsule biosynthesis; mutants have growth defect at 37 C and are sensitivity to SDS and hyperosmotic stress CNAG_06576 allergen All cytosolic protein involved in capsule formation; loss of All1 confers hypervirulent phenotype via modification of macrophage-mediated phagocytosis and T-cell-mediated immune responses Stress-related and heat shock proteins/chaperones (5) CNAG_03482 thioredoxin-dependent peroxide thiol-specific antioxidant; homolog of ScTsa1 reductase CNAG_06267 Rds1 protein stress-related protein regulated by iron availability; contains ferritin-like domain CNAG_01727 Hsc heat shock protein CNAG_06150 heat-shock protein heat shock protein

5 CNAG_03621 cyclophilin A chaperone Intracellular traffic, vacuole function (3) CNAG_02568 UBA/TS-N domain-containing protein endocytosis-related protein; contains Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction CNAG_03251 ARF small monomeric GTPase Arf1 regulates the formation of COPI vesicles and of clathrin-coated vesicles; COPI-coated vesicles are involved in retrograde traffic from the Golgi; and the clathrin polymeric coat combines with various adaptor complexes to mediate budding from the trans-golgi network and endosomal compartments, as well as from the plasma membrane CNAG_03316 Rho GDP-dissociation inhibitor inhibits RhoGTPase to regulate vacuolar fusion Unclassified proteins (17) CNAG_02585 RfeF Von Willebrand factor type A (vwa) domain protein CNAG_01896 aldehyde reductase I belongs to a superfamily of soluble NAD(P)(H) oxidoreductases whose chief purpose is to reduce aldehydes and ketones to primary and secondary alcohols CNAG_01375 conserved hypothetical protein actin regulatory protein CNAG_01854 heparinase II/III family protein putative heparinase CNAG_00886 adenosylhomocysteinase key regulator of methylation CNAG_02748 UTP-glucose-1-phosphate carbohydrate metabolism uridylyltransferase CNAG_00935 curved DNA-binding protein Cdb metallopeptidase family M24 domain CNAG_02974 voltage-dependent ion-selective channel voltage-dependent anion channel CNAG_01653 cytokine inducing-glycoprotein Cig extracellular mannoprotein involved in iron acquisition from heme CNAG_00483 actin cytoskeleton CNAG_02285 nucleoside-diphosphate kinase maintain cellular pool of NTPs

6 CNAG_05258 glucose-methanol-choline GMC oxidoreductase, melanin biosynthesis oxidoreductase Smg1 CNAG_02545 inorganic diphosphatase hydrolyzes inorganic pyrophosphate to two molecules of orthophosphates CNAG_00021 adenosylmethionine decarboxylase required for biosynthesis of spermidine and spermine in S. cerevisiae CNAG_02752 short chain dehydrogenase glucose/ribitol dehydrogenase CNAG_04016 conserved hypothetical protein Ferritin-like protein related to stress response protein Rds1 CNAG_01182 cytoplasmic protein KH domain RNA binding protein Hypothetical proteins (10) CNAG_00982 conserved hypothetical protein RNA recognition motif-containing protein CNAG_01796 conserved hypothetical protein hypothetical protein CNAG_02422 conserved hypothetical protein hypothetical protein CNAG_04066 endoribonuclease L-PSP putative translation initiation inhibitor CNAG_06109 conserved hypothetical protein hypothetical protein CNAG_06075 conserved hypothetical protein SBDS domain protein CNAG_00586 conserved hypothetical protein hypothetical protein CNAG_03007 conserved hypothetical protein hypothetical protein CNAG_04785 conserved hypothetical protein stress responsive A/B barrel domain protein CNAG_06113 conserved hypothetical protein Stm1 and Hyaluronan/mRNA-binding domains protein