Paper ID: ART

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1 analytical process. Delayed sample analysi changes of measured parameters co interpretation of results. Pre-analytical variables, such as stor temperature affect the measurement parameters collected in EDTA.Laboratory aware of the changes that occur during specific setting in order to decide whether to samples that are too old to obtain reliable r measurement of Complete blood coun differential count (DIFF) as well as periphe (PBS) morphology are essential for the corre of haematology results. Parameters useful for diagnosis and haematological disorders, such as mean cell and PBS morphology are unreliable after 12 swelling of red cells during storage at Ro affects volume-dependant variables an

2 count with automated differential count is s at least 24 h or even to 72 h, with signifi depending on the type of automated blood particular, the monocyte count tends to eosinophil and lymphocyte counts tend to time; neutrophil count is stable up to 72 h generation instruments. A recent study, by Daves, Zaglereta SYSMEX XN, suggested no meaningful bia after 3 h under different storage conditions blood cell distribution width (RDW) and (impedance technique, PLT-I) at 37 meaningful bias was observed for me haemoglobin (MCH) and mean corpuscular at room temperature, red blood cell (RBC corpuscular haemoglobin concentration (M MCV and PLT-I at 4 C, and RBC, RDW and PLT-I at 37 C. After 24 h, a mean

3 Exclusion Criteria: Blood sample collected indoor (MKCG M Blood samples received from primary health care centres. (I.e. blood samples w storage time and condition.) Blood samples received from perip verification. Procedure 88 randomly selected blood samples venepuncture and anticoagula dipotassiumethylenediamine tetra-acetic aci measured within 1 h of collection onsys (installed in the section of haematology un of Pathology, MKCG Medical College, B sample was taken as the baseline (0 h) sam measurements were made at 5, 24, and 48 baseline measurement.

4 showed significant decreases (P<0.05) at collection.neutrophil count was stable up increased slightly in 48h sample. The s contained too few basophils to obtain reli basophil stability. EDTA-induced changes w hours after collection, which precluded a ma count. Morphological Changes in Ps (Perephera Depending on the time the peripheral b prepared, morphological changes were seen all Cells. Some neutrophils showed nuclea changes in the chromatin, that is homogenously compared to fresh nuclei, structure of the lobes that may become cytoplasmic rim appeared ragged or less w vacuolization and loss of granules may be ob shrinkage, chromatin condensation in

5 collection when stored at room temperature significant decrease in WBC count was ob due to degenerative changes in cells).how changes were observed for TWBC in refri up to 48h. Platelets were only stable up to 24 hours when stored at Room temperature. Ti concentration-related changes in PLT shape spherical and swelling do occur in specim EDTA; as a result of these changes, the me (MPV) is not a fully reliable value after 24 at room temperature. Interestingly, MPV v refrigerated sample showed increased dev control value in 24h sample as compared to temp. Storage of samples at 4 C increased the s parameters. CBC parameters, namely WBC

6 MCV(FL) MCH(pg.) MCHC(g/dl) RDW (%) PLT(10 9 /L) MPV(Fl) RET% Table 4: Mean, mean percentage change a Count on s n=88 FRESH 5 hr. Change% (<1hr) Lo NEUT LYMPH MONO EO BASO

7 Figure 3: (variation of Haemoglobin% w storage time, in both incubated and refrige

8 Figure 11: (variation of Differenti Figure 12: (variation of Differential C

9 Figure 15: (variation of Total Eosinophi prolonged storage time, in both incubated a sample) Figure 16: (variation of Total Neutrophi prolonged storage time, in both incubated a sample)

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