Separations. Sweet Sugar. Are in Your Future. New. Luna Omega SUGAR LC Column OMEGA

Transcription

1 OMEGA Sweet Sugar Separations Are in Your Future New Luna Omega SUGAR LC Column Improved carbohydrate retention and separation with novel HILIC selectivity Enhanced lifetime with highly robust and efficient thermally modified fully porous particle QC tested for sugars to ensure reliable quality

2 The Sugary Dilemma Over the past year, we ve worked and spoken with a large number of customers in regards to sugar analysis from all kinds of different matrices. Turns out that traditional amide and ao phases were just not hitting that SWEET spot for simple sugars. They were actually causing quite a number of customers to HIT THE WALL because of: Very poor column lifetime and stability Retention time variability Lackluster separation between key sugars Low response and performance Need for complex mobile phase systems to gain retention Long run times Poor retention of highly polar compounds 8 Phenomenex, Inc. All rights reserved.

3 A Tasty Result With Luna Omega SUGAR With so many problems, a unique solution was needed! So, we took all this knowledge and feedback and combined it with our 6 years of separation science technology and experience. Now it s time for you to take our newest creation for a taste test! The New Particle, New Phase Just For Sugars! Novel stationary phase... p. Exceptional retention and separation of sugars... p. 5 Simple HILIC operating conditions for RI or ELSD... p. 5 High reproducibility... p. 6 Brilliant column robustness and stability... p. 7 Sugars in Food and Beverages Milk (Non-fat, %, % Lactose Free)... p. 8 Infant Formula... p. 8 Dark Chocolate... p. 9 Ketchup... p. 9 Merlot Wine... p. White Wine... p. Low Sugar White Wine... p. Dessert Wine... p. Grape Soda... p. Regular Cola... p. Diet Soda... p. Peach Iced Tea... p. Orange Juice... p. Apple Juice... p. Yogurt Drink... p. Buttermilk... p. Sugars and Sugar Alcohols... p. Sugars in Pharmaceuticals and Formulations Cough Syrup... p. Tablets... p. Sugars in Agriculture Horse Feed... p. 5 Livestock Feed... p. 5 Method Development Tips Effect of flow rate with RI detection... p. 6 Effect of temperature on separation... p. 7 Easy Sample Filtering Syringe Filter Selection... pp. 8-9 QuEChERS, SLE, and SPE... p. Simple Vial Selection... p. Column Protection... p. Ordering Information... p.

4 The New Particle and New Phase Just For Sugar! The new Luna Omega SUGAR breaks ground as it combines the performance benefits of thermally modified fully porous particles with a novel HILIC stationary phase that excels at polar compound retention and selectivity. Cutting Edge Fully Porous Silica Particle Luna is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, reproducibility, and dependability for a wide range of analyses. The new Luna Omega builds upon this legacy with an innovative yet rugged silica particle architecture, designed and manufactured by Phenomenex based on more than years of applied knowledge, invention, and customer experience. Novel Design and Manufacturing Process Within the novel manufacturing process of Luna Omega silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity. Micropores Consistent Porosity Thermally Modified Pore Structure Most importantly, through our proprietary process, we eliate micropores, further improving column efficiency, inertness, and reproducibility. Amide Polyol Aq Ao Aq App ID 7 Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: ELSD Sample:. Fructose. Glucose. Galactose. Sucrose 5. Maltose 6. Lactose Novel nitrogen containing stationary phase that greatly increases the retention of sugars and sugar alcohols under HILIC conditions

5 Exceptional Retention and Separation Luna Omega SUGAR greatly improves upon the retention and separation capabilities of traditional fully porous, core-shell, and hybrid materials, while also allowing for greater peak response! All this while also ensuring that customers do not need to depend on buffers or ion pair agents to get adequate separation at the cost of losing signal. Luna Omega μm SUGAR Fully Porous μm NH, 5, Hybrid Fully Porous.5 μm Amide App ID 767 App ID 768 Excellent Simple Sugar Separation! Conditions for all columns: Column: Luna Omega μm SUGAR Fully Porous μm NH Hybrid Fully Porous.5 μm Amide Dimension: 5 x.6 mm Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: 5 C Detection: RI Sample:. Fructose. Glucose. Galactose. Sucrose 5. Maltose 6. Lactose 75 5 Peak Splitting and Poor Resolution! 5 5,6 App ID Simplified HILIC Conditions for RI or ELSD Why make things tough? While making the new Luna Omega SUGAR we focused on simplified HILIC mobile phase systems that would work with all common detectors including RI, ELSD, and MS. Additionally, the high organic content decreases interference as non-polar compounds and contaants are forced to elute early in the run. 8 6 Simple Mobile Phase ACN H O Comparative separations may not be representative of all applications. 5 App ID 78 Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose. Sucrose. Maltose 5. Lactose 5

6 High Reproducibility Luna Omega SUGAR media and columns are designed to be consistent and incredibly accurate tools for sugar analysis by HPLC and UHPLC. Each batch and column is specifically tested for the analysis of simple sugars to confirm proper selectivity, alongside a large number of other tests to ensure performance, particle quality, dependability, and overall reproducibility Zante Currants Batch A 5 Batch B 5 App ID 856 App ID 856 Conditions for all columns: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: 5 C Detection: ELSD Sample:. Fructose. Glucose Batch C App ID 856 Sample Preparation with Phenex Nylon Syringe Filter. 5 g of each material (Goji Berries, Zante Currants, and Turkish Apricots) frozen at -8 C for hour. Use spice grinder to homogenize the samples. Place the material in a 5 ml beaker and add 5 ml of DI water and heat to 5 C with a stir-bar for utes. Add 5 ml of Chloroform and mix on high for 5 at 5 C 5. Let solution come to rest at room temperature to allow two phases to form 6. Decant top (aqueous) layer to centrifuge tube, dispose of bottom layer 7. Centrifuge at 6 RPM 8. Decant into new ml scintillation vials 9. Filter using.5 μm Phenex Nylon syringe filter. Inject 5 μl 6

7 Brilliant Column Robustness and Stability A major concern for a lot of customers (maybe even yourself) was the short life span of their traditional amide and ao columns. With the Luna Omega SUGAR, we focused heavily on combined particle and stationary phase robustness to greatly imize efficiency and retention loss over time. Stability Comparison of Fully Porous Materials Luna Omega μm SUGAR Conventional Fully Porous μm NH % % % of Initial Retention 8% 6% % Improved particle robustness drives greater stability! % % Injection Cycle Extend Column Lifetime Even MORE with a SecurityGuard Guard Cartridge System! Fingertight connection to virtually any female inverted endfitting worldwide Contaants Trapped A B Cutaway view showing cartridge can be easily inspected for contaants Universal fingertight connection to HPLC column no wrenches required contaants compounds of interest A B From Injector SecurityGuard Standard HPLC Column to Detector See page for details 7

8 Food and Beverages Milk Samples Non-Fat Milk 5 Consistent lactose retention in a variety of milk samples! 5 App ID % Fat Milk 5 5 App ID % Fat Milk 5 5 Low level galactose response by Luna Omega SUGAR! 5 App ID Lactose Free Milk 5 5 App ID Infant Formula App ID Conditions for all samples: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: 5 C Detection: ELSD Sample:. Glucose. Galactose. Lactose Phenomenex. l

9 Food and Beverages Chocolate and Ketchup Dark Chocolate Extract 8 6 App ID Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Sucrose. Lactose Ketchup Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose App ID 7 Tip! Quick and Easy Sample Filtration with Phenex Nylon Syringe Filters! See page 8-9 9

10 Food and Beverages Wine 5 Merlot White Wine App ID App ID 7 Gewurztraer Wine Selectivity consistent across different samples! App ID 7 5 Dessert Wine App ID 78 Conditions for all samples: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose

11 Food and Beverages Soda Grape Soda 5 5 Conditions for Grape Soda: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose 5 App ID Regular Cola App ID 79 5 Diet Cola Exciting yet boring chromatogram with no presence of sugar! App ID 79 Conditions for Cola: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (8:) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose

12 Food and Beverages Iced Tea and Juice Peach Iced Tea Orange Juice Conditions for Peach Iced Tea: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose. Sucrose Conditions for Orange Juice and Apple Juices: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (8:) Flow Rate: ml/ Temperature: C Detection: RI Sample:. Fructose. Glucose. Sucrose App ID 75 App ID 77 5 Apple Juice App ID 78

13 Food and Beverages Yogurt, Buttermilk, and Sugar Alcohols Buttermilk Conditions for Buttermilk and Yogurt Drink: 8 Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm 6 Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: ELSD Sample:. Sucrose. Lactose App ID 7 8 Yogurt Drink App ID 75 Simple Sugars App ID Sugar Alcohols 6 App ID 77 Conditions for Sugar and Sugar Alcohols: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: A: Water B: Acetonitrile/Isopropanol/Water (9:5:5) Gradient: Time () % B Flow Rate: ml/ Temperature: 5 C Injection Volume: 5 μl Detection: ELSD Sample:. Xylitol 6. Mannitol. Arabitol 7. Galactose. Fructose 8. Sucrose. Sorbitol 9. Maltose 5. Glucose. Lactose

14 Pharmaceuticals Cough Syrup and Tablet Ibuprofen Tablet Cough Syrup - Children's Fever Reducer Fructose Sorbitol Sucrose App ID 79 App ID 79 Conditions for all samples: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: A: Water B: Acetonitrile/Isopropanol/Water (9:5:5) Gradient: Time () % B Flow Rate: ml/ Temperature: 5 C Injection Volume: 5 μl Detection: ELSD Sample: As Noted A Better Search! Easily find applications, product guides, and technical tips through our new search tool!

15 Agriculture Animal Feed -Grain Horse Feed App ID 78 Animal Feed and Molasses App ID 78 Conditions for all samples: Analytical Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: A: Water B: Acetonitrile/Isopropanol/Water (9:5:5) Gradient: Time () % B Flow Rate: ml/ Temperature: 5 C Injection Volume: 5 μl Detection: ELSD Sample:. Fructose. Glucose. Sucrose 5

16 Method Development Tips Flow Rate With the fine-tuned selectivity of Luna Omega SUGAR for simple sugars, you have the ability to adjust the flow rate if needed for less system stress or for speeding up the analysis to improve throughput ml/ ml/ App ID 7 App ID ml/ App ID ml/ App ID 7 Conditions for all samples: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: 5 C Detection: RI Sample:. Fructose. Glucose. Sucrose. Maltose 5. Lactose 6

17 Method Development Tips Temperature In this illustration you can see the direct effect of temperature on the separation and peak shape. With the increase in temperature, the analysis time shortens and peaks start to sharpen up. This can be quite advantageous when looking to improve productivity, especially without the need to adjust column length C App ID C App ID C App ID C App ID 76 Conditions for all samples: Column: Luna Omega μm SUGAR Dimension: 5 x.6 mm Part No.: F-775-E Mobile Phase: Acetonitrile/Water (75:5) Flow Rate: ml/ Temperature: C Detection: ELSD Sample:. Fructose. Glucose. Galactose. Sucrose 5. Maltose 6. Lactose 7

18 Easy Sample Filtration Phenex Syringe Filters Rapid filtration of LC samples prior to analysis Particulate, PVC, and extractable-free filters Less system downtime More consistent, reproducible results Increased column lifetime Syringe Filter Selection Guide. Choose filter diameter based on sample volume ml Sample Volume - ml Sample Volume - ml Sample Volume mm Diameter 5 mm Diameter 5-8 mm Diameter. Choose a pore size based on the nature of your sample and chromatographic method Sample Description General aqueous or mixed organic samples prior to LC analysis with columns packed with > μm particles. General clarification of GC, SFC, CE, and GPC samples. Viscous samples or samples containing high levels of particulate matter. Recommended Filter Pore Size.5 μm General aqueous or mixed organic samples prior to LC analysis with columns packed with < μm particles. Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples.. μm Gas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods. Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such as some environmental, biofuels or food and beverage applications. Glass Fiber Filter with.5 μm filter membrane. Choose a filter membrane according to the characteristics of your sample and filtering objective Membrane Type RC (Regenerated Cellulose) PTFE, Teflon (Polytetrafluoroethylene) PES (Polyethersulfone) NY (Nylon) CA (Cellulose Acetate) PVDF (Polyvinylidene Fluoride) GF (Glass Fiber) Recommended Uses Hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions Phenex-RC filters also exhibit fast-flow and ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, Phenex-RC membranes are broadly recommended as an excellent general purpose/high-performance sample filter for most applications. PTFE is an inherently hydrophobic membrane excellent for filtration of organic-based, highly acidic or basic samples and solvents especially well suited for the clarification of non-aqueous samples. Although this membrane is hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water. Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many life science clarification applications. Phenex-PES membranes typically offer better chemical resistance than cellulose acetate and are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers. Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic samples. In combination with a glass pre-filter (Phenex-GF/NY), this membrane is excellent for the filtration of particle-laden samples, such as foods and beverages, environmental, biofuels, and dissolution samples. Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In combination with a glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological sample filtration. Hydrophilic PVDF membrane provides high flow rates and throughput, low extractables, and broad chemical compatibility. This membrane binds less protein than nylon or PTFE membranes. Glass Fiber (GF) filters are made of inert borosilicate glass and have a noal. μm pore size. They are commonly used with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples, soil samples, fermentation broth samples, removal of yeasts, molds, etc.). Glass Fiber filters can be used alone or in series with other Phenex filter membranes such as the.5 μm pore Phenex-RC filter to reduce clogging of the membrane and optimize flow. 8

19 Easy Sample Filtration Phenex Syringe Filters Tip: Try a Sample Pack! Request yours today by phone or visit If Phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, return the product with comparative data within 5 days for a FULL REFUND. mm Diameter for ml sample volumes 5 mm Diameter for ml sample volumes 5-8 mm Diameter for ml sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price. μm.5 μm. μm RC (Regenerated Cellulose) PES (Polyethersulfone) PTFE 6 (Polytetrafluoroethylene) NY (Nylon) GF/NY (Glass Fiber/Nylon) PVDF (Polyvinylidene Fluoride) GF/PVDF (Glass Fiber/Polyvinylidene Fluoride) CA (Cellulose Acetate) GF/CA,, (Glass Fiber/Cellulose Acetate) RC (Regenerated Cellulose) PES (Polyethersulfone) PTFE 6 (Polytetrafluoroethylene) NY (Nylon) GF/NY (Glass Fiber/Nylon) PVDF (Polyvinylidene Fluoride) GF/PVDF (Glass Fiber/Polyvinylidene Fluoride) GF/CA,, (Glass Fiber/Cellulose Acetate) GF, (Glass Fiber) AF-- /pk AF-- /pk AF-8-5 /pk AF--5 5/pk AF--5 5/pk AF /pk AF-88-7 /pk AF /pk AF-- /pk AF-- /pk AF-- /pk AF--5 5/pk AF--5 5/pk AF--5 5/pk AF-7- /pk AF-7- /pk AF-7- /pk AF-7-5 5/pk AF-7-5 5/pk AF-7-5 5/pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF-A7-7 /pk (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the AF-A /pk most difficult samples. Outlet connection is luer lock. AF /pk AF6-66- /pk AF /pk AF /pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a PVDF AF6-6C6- /pk membrane. The hydrophilic PVDF membrane provides high flow rates and throughput, low extractables and broad chemical compatibility. This membrane binds less protein than nylon or AF6-6C6-5 5/pk PTFE membranes. AF-8-7 /pk AF /pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF-8A9-7 /pk membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification. Outlet connection is luer lock. AF-8A /pk AF-- /pk AF-- /pk AF-8-5 /pk AF--5 5/pk AF--5 5/pk AF /pk AF-88-7 /pk AF /pk AF-- /pk AF-- /pk AF-- /pk AF--5 5/pk AF--5 5/pk AF--5 5/pk AF-7- /pk AF-7- /pk AF-7- /pk AF-7-5 5/pk AF-7-5 5/pk AF-7-5 5/pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF-B7-7 /pk (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, AF-B7-5 environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the 5/pk most difficult samples. Outlet connection is luer lock. AF /pk AF6-66- /pk AF /pk AF /pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a PVDF membrane. The hydrophilic PVDF membrane provides high flow rates and throughput, low AF6-6D6- /pk extractables and broad chemical compatibility. This membrane binds less protein than nylon or PTFE membranes. AF6-6D6-5 5/pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF-8B9-7 /pk membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification. Outlet connection is luer lock. AF-8B /pk Prefiltration of heavily contaated or highly viscous samples. When used in-series preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. Outlet connection is luer lock. AF /pk AF /pk. Larger quantity purchases at significant savings are available.. Glass fiber filters are 8 mm diameter and made of borosilicate. They will remove 9 % of all particles >. μm.. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite.. Cellulose acetate is surfactant-free mm diameter. 6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA mm diameter mm diameter. 9. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP), and offer luer lock inlet/slip outlet connections, unless otherwise indicated. 9

20 Need More Selective Sample Prep Depending on your sample amounts, types, and productivity needs, we offer a full suite of sample preparation solutions covering common techniques such as QuEChERS, SLE, and SPE. QuEChERS Supported Liquid Extraction Solid Phase Extraction Polymer-Based & Silica-Based Decrease LC-MS Down Time for Maintenance Increase Column Lifetime Remove Particulates Remove Proteins Remove Phospholipids De-salt Solvent Switching Specifically Extract Target Analytes Concentrate Product Recommendation novum simplified liquid extraction Polymeric SPE Supported Liquid Extraction Solid Phase Extraction Clean-up Time () < < 5 < Degree of Cleanliness

21 Quick Vial, Cap, and Kit Selection Certified Vial Products From start to finish, Verex Certified vial and cap products are manufactured to provide high quality, state-of-theart solutions for your most challenging, sensitive applications. Our innovative precision products offer compatibility with virtually any autosampler and provide air-tight, leak-free seals to safely store and transfer your most important samples. Verex vials are guaranteed for % satisfaction. All Vials, Caps, and Kits Advanced manufacturing Septum Cap Multi-step QA/QC Cleanroom packaged Certified quality Vial Make the Switch from your Current Vials and Caps to Verex! Easy Steps: Enter your current vial or cap part number Confirm the correct part number was entered Order the recommended Verex part numnber Finding the Verex replacement to your current vials and caps is EASY. Use our online web tool to find the guaranteed Verex product match. Visit:

22 Protect Your Luna Omega SUGAR Column The easiest way to extend column performance is to prevent contaants and particulates from getting into your Luna Omega column with the SecurityGuard Standard guard cartridge system. Protects and extends column lifetimes Virtually no change in chromatography Simple to use HPLC Column Protection From Injector Fingertight connection to virtually any female inverted endfitting worldwide 9 Cartridge Replaced 8 Guard Cartridge System HPLC Column to Detector A B Contaants Trapped A B Cutaway view showing cartridge can be easily inspected for contaants Universal Fingertight connection to HPLC column no wrenches required contaants compounds of interest Backpressure (Bar) Injections Column returns to initial backpressure Accelerated lifetime test using endogenous biomolecule matrix on a 5 μm 5 x.6 mm column with SecurityGuard cartridges. Backpressure values represent additional backpressure contributed by SecurityGuard. The SecurityGuard analytical cartridge holder (patented) directly finger-tightens into virtually any manufacturer s column endfitting. Contaants are retained by an inexpensive disposable cartridge instead of damaging your valuable HPLC and SFC column investment. Simply replace SecurityGuard cartridges instead of your expensive columns. In this graph, once the expired SecurityGuard cartridge was replaced, the pressure immediately dropped and the column performance was restored allowing for extended column use. See it in action:

23 Luna Omega SUGAR Ordering Information SecurityGuard μm Minibore Columns (mm) Cartridges (mm) Phases 5 x. x. 5 x. x.* /pk Sugar B-775-AN D-775-AN F-775-AN AJ-96 for ID:.-. mm SecurityGuard μm MidBore Columns (mm) Cartridges (mm) Phases 5 x. x.* /pk Sugar F-775-Y AJ-96 for ID:.-. mm SecurityGuard μm Analytical Columns (mm) Cartridges (mm) Phases x.6 5 x.6 5 x.6 x.* /pk Sugar D-775-E F-775-E G-775-E AJ-95 for ID:.-8. mm * SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, return the column with comparative data within 5 days for a FULL REFUND. Additional Selectivities Luna Omega also comes in three reversed phase selectivities for UHPLC, HPLC, and Preparative applications. Find out more at TMS TMS TMS Polar TMS Polar + TMS TMS + C8 Polar C8 PS C8 Rugged and highly efficient C8 with strong focus on hydrophobic retention of non-polar and polar compounds % aqueous stability and enhanced selectivity/retention for polar analytes without diishing useful non-polar retention. The C8 ligand provides general hydrophobic interactions while a polar modified particle surface provides enhanced polar compound retention. Unique, % aqueous stable mixed-mode phase that provides both polar and non-polar retention. The surface contains a positive charged ligand which aids in the retention of acidic compounds through ionic interactions, while the C8 ligand promotes general reversed phase retention. The positively charged surface also improves basic compound peaks shape through ionic repulsion.

24 OMEGA New Luna Omega SUGAR Improved carbohydrate retention and separation Excellent lifetime and robustness Australia t: +6 ()-98-6 Austria t: + ()-9- anfrage@phenomenex.com Belgium t: + () 5 5 (French) t: + () (Dutch) beinfo@phenomenex.com Canada t: + (8) 5-68 info@phenomenex.com China t: cninfo@phenomenex.com Denmark t: nordicinfo@phenomenex.com Finland t: +58 () nordicinfo@phenomenex.com France t: + () 9 franceinfo@phenomenex.com Germany t: +9 () anfrage@phenomenex.com India t: +9 ()- indiainfo@phenomenex.com Ireland t: +5 () 7 55 eireinfo@phenomenex.com Italy t: italiainfo@phenomenex.com Mexico t: tecnicomx@phenomenex.com The Netherlands t: + ()-87 nlinfo@phenomenex.com New Zealand t: +6 () nzinfo@phenomenex.com Norway t: nordicinfo@phenomenex.com Portugal t: ptinfo@phenomenex.com Singapore t: sginfo@phenomenex.com Spain t: esp info@phenomenex.com Sweden t: +6 () nordicinfo@phenomenex.com Switzerland t: swissinfo@phenomenex.com United Kingdom t: + () ukinfo@phenomenex.com USA t: + () -555 info@phenomenex.com All other countries Corporate Office USA t: + () -555 info@phenomenex.com Luxembourg t: + ()-87 nlinfo@phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com BR59778_W Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at Trademarks Luna and Strata are registered trademarks, roq, Novum, MidBore, SecurityGuard, Phenex, and Verex are trademarks of Phenomenex. Teflon is a registered trademark of The Chemours Company FC, LLC. Cyrolite is a registered trademark of Evonik Cyro LLC. Disclaimer Comparative separations may not be representative of all applications. Strata-X is patented by Phenomenex. U.S. Patent No. 7,9,5 SecurityGuard is patented by Phenomenex. U.S. Patent No. 6,6,6 CAUTION: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP or ULTRA holders, or to any cartridges. Novum is patent pending. FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures. 8 Phenomenex, Inc. All rights reserved.