2016 PoLA Gross Exam 8/4/ Rat. Etiology: Sialodacryoadenitis virus (rat coronavirus) 3. Mouse. Morphologic diagnosis(es):

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1 2016 PoLA Gross Exam 1. Rat. Sialodacryoadenitis virus (rat coronavirus) See Percy and Barthold, 4 th ed., fig 2.17 AFIP Cynomolgus macaque. Give two differential etiologies: Cercopithecine herpesvirus-9 (simian varicella) Macacine herpesvirus-1 (B virus) Measles virus (human morbillivirus) Schoeb TR, Eberle R, Black DH, Parker RF, Cartner SC. Diagnostic exercise: papulovesicular dermatitis in rhesus macaques (Macaca mulatta). Vet Pathol Jul;45(4): PMID: Mouse. Morphologic diagnosis(es): Head, cranium bifidum, with hydrocephalus/hydranencephaly and meningocele/encephalocoele 1

2 4. Rabbit. Morphologic diagnosis: Coxofemoral joints, hip dysplasia Name one risk factor: Improper caging/flooring for kits 5. Zebrafish. Etiologic Diagnosis: Neural microsporidiosis Name the disease: Skinny disease 6. Cynomolgus macaque. Morphologic diagnosis(es): Lung, right cranial lobe, granuloma, focally extensive, chronic, moderate Liver, granulomas, multifocal to coalescing, chronic, moderate Mycobacterium tuberculosis 7. Mouse. Morphologic diagnosis: Mammary gland, tumor, adenocarcinoma Second most common non-lymphoid tumor in NOD mice after osteosarcoma Kavirayani AM, Foreman O. Retrospective study of spontaneous osteosarcomas in the nonobese diabetic strain and nonobese diabeticderived substrains of mice. Vet Pathol May;47(3): PMID:

3 8. Pig. Cyst of Balantidium coli 9. B6.129S7-Rag1 tm1mom /J Mouse. Pneumocystis murina Name a special stain that would be useful: Gomori s methenamine silver (GMS) Periodic acid Schiff (PAS) 10. Rabbit. Give three differential etiologies: Rotavirus (A) Enteric coronavirus Clostridium spiroforme C. difficile C. perfringens C. piliforme Enteropathogenic E. coli Enterohemorrhagic E. coli Lawsonia intracellularis Coccidia, esp. highly pathogenic Eimeria intestinalis E. flavescens Salmonella sp Rat. Morphologic diagnosis: Pituitary, pars distalis, tumor, adenoma Name/describe a likely positive immunohistochemical result: Prolactin positive 3

4 12. Woodchuck. Morphologic diagnosis: Liver, hepatitis, chronic, moderate to severe Woodchuck hepatitis virus (hepadnavirus) 13. Rabbit. Name the lesion: Glaucoma/buphthalmia Genetic, autosomal recessive with incomplete penetrance 14. Mouse. Morphologic diagnosis: Rectal prolapse, acute Provide three differential etiologies: Syphacia obvelata Aspiculuris tetraptera Citrobacter rodentium Helicobacter hepaticus, H. bilis, H. muridarum, H. rodentium, H. typhlonius, H. ganmani, H. rappini, H. mastomyrinus 15. Ferret. Morphologic diagnosis: Stomach, ulcers, bleeding, multifocal Helicobacter mustelae 4

5 16. Mouse. Morphologic diagnosis: Skin, dorsal pelvis, lacerations, multifocal, acute Name a likely strain: Albino SJL/J BALB/c or BALB/cBy 17. Rabbit. Name the disease: Mucoid enteropathy 18. Mouse. Give two differential etiologies: Helicobacter hepaticus H. bilis [Other Helicobacter] Name the histochemical stain shown here: Steiner or Warthin-Starry 19. Rabbit. Kappapillomavirus-2/Shope papillomavirus/cottontail rabbit papillomavirus 5

6 20. Xenopus. Morphologic diagnosis: Skin, cutaneous infarcts, multifocal, acute-subacute, severe Name the disease: Redleg Most likely etiology: Aeromonas hydrophila ddx: Proteus sp., E. coli, Aerobacter sp. Pseudomonas sp., Citrobacter sp., Mimi sp., Staphylococcus sp., Streptococcus sp., Enterobacter sp., Klebsiella sp., Chryseobacterium sp. 21. AFIP Rhesus macaque 21. Rhesus macaque. Pathogenesis: Genetic deficiency of l-gulono-1,4-lactone oxidase + insufficient dietary ascorbic acid (vitamin C) inactivity of prolyl and lysyl hydroxylases inability to post-translationally form hydroxyproline and hydroxylysine inability to cross-link fibrillar collagens (I, II, III) and elastin reduced strength of vascular walls, tendons, ligaments, bone, cartilage spontaneous hemorrhage and ligament/tendon tearing Name another susceptible laboratory animal: Any other non-human primate; guinea pig 22. Mouse. Morphologic diagnosis: Heart, epicardial mineralization, multifocal to coalescing, chronic, moderate Name a likely strain: BALB/c or BALB/cBy Including C.B.-17-scid C3H DBA/1 or DBA/2 Also KK/HIJ A/J C57BL/10J 6

7 23. Rat. Morphologic diagnosis: Lungs, chronic bronchitis/bronchiolitis, suppurative, MF, chronic/chronic-active, severe with bronchiectasis/ bronchiolectasis Mycoplasma pulmonis Name the disease: Murine respiratory mycoplasmosis/chronic respiratory disease 24. Goat. Morphologic diagnosis: Lip/mouth, cheilitis/stomatitis, proliferative and ulcerative, focally extensive, subacute, moderate Orf virus (type species of parapoxvirus) 25. Mouse. Morphologic diagnosis: Dorsum, subcutaneous emphysema, diffuse, acute, severe Most likely etiology: Trauma (gavage accident) 26. Rabbit. Treponema paraluiscuniculi Name the disease: Rabbit syphilis/vent disease 7

8 27. Rat. Morphologic diagnosis: Zymbal s gland, tumor, adenocarcinoma 28. Mouse. Morphologic diagnosis: Haired skin, acanthosis and orthokeratotic hyperkeratosis, diffuse, moderate Corynebacterium bovis [Staphylococcus xylosus] 29. Rabbit. Morphologic diagnosis(es): Uterine horn, tumor, endometrial adenocarcinoma with ipsilateral hydro- /mucometra (+/- cystic endometrial hyperplasia) 30. Pig. Ascaris suum Cannot be readily distinguished from A. lumbricoides (human large roundworm) 8

9 31. Mouse. Morphologic diagnosis: Lymph nodes, tumor, lymphoma, multicentric 32. Pig. Morphologic diagnosis: Lung, right, bronchopneumonia, focally extensive, chronic, moderate Most likely etiology: Mycoplasma hyopneumoniae 34. Guinea pig. Morphologic diagnosis: 33. Zebrafish. Pseudoloma neurophilia Name a special stain that would be useful: Ziehl-Neelsen acid fast Brown and Hopps or Brown and Brenn tissue Gram stain Carbol Fuchsin Luna (PMID: ) Eye, ciliary body, choristoma, osseous (heterotopic bone) Griffith JW, Sassani JW, Bowman TA, Lang CM. Osseous choristoma of the ciliary body in guinea pigs. Vet Pathol Jan;25(1): PMID: Brooks DE, McCracken MD, Collins BR. Heterotopic bone formation in the ciliary body of an aged guinea pig. Lab Anim Sci Jan;40(1): PMID: Donnelly TM, Brown C, Donnelly TM. Heterotopic bone in the eyes of a guinea pig: osseous choristoma of the ciliary body. Lab Anim (NY) Jul-Aug;31(7):23-5. PMID:

10 35. Pigtail Macaque. Morphologic diagnosis: Colon, tumor, adenocarcinoma Harbison CE, Taheri F, Knight H, Miller AD. Immunohistochemical Characterization of Large Intestinal Adenocarcinoma in the Rhesus Macaque (Macaca mulatta). Vet Pathol Jul;52(4): PMID: Kerrick GP, Brownstein DG. Metastatic large intestinal adenocarcinoma in two rhesus macaques (Macaca mulatta). Contemp Top Lab Anim Sci Jul;39(4):40-2. PMID: Valverde CR, Tarara RP, Griffey SM, Roberts JA. Spontaneous intestinal adenocarcinoma in geriatric macaques (Macaca sp.). Comp Med Oct;50(5): PMID: Rat. Name the lesion: Malocclusion 37. Pig. Morphologic diagnosis: Stomach, pars esophagea, ulcer, subacute, severe Name/describe two risk factors: Finely ground feed (<700 um) Stress Vitamin E/Se deficiency Helicobacter infection Cu deficiency Whey/milk diet 38. Rabbit. Eggs of Passalurus ambiguus (rabbit pinworm) 10

11 39. Mouse. Morphologic diagnosis: Liver, hepatitis, necrotizing and histiocytic, multifocal, acute, moderate with syncytia Mouse hepatitis virus (murine coronavirus) 40. Rabbit. Morphologic diagnosis: Lung, right cranial and accessory lobes, bronchopneumonia, suppurative, focally extensive, moderate with abscessation Provide two differential etiologies: Pasteurella multocida Bordetella bronchiseptica 41. Owl monkey. Morphologic diagnosis: Mouth/tongue: Stomatitis/glossitis, pustular and vesicular, multifocal, acute, moderate to severe Most likely etiology: Saimiriine herpesvirus-1 (SaHV1, aka herpes T, simian herpes-4, herpes tamarinus) Name the reservoir host: Squirrel monkey AFIP Rat. Name the disease/lesion: Polyarteritis nodosa 11

12 43.Guinea pig. Morphologic diagnosis: 44.Rhesus macaque. Pathogenesis: Stomach and spleen, gastric dilatation and volvulus, acute, severe Type IV (delayed type (DTH)) hypersensitivity response to intradermal tuberculin administration (positive tb test) 46. Sheep 45. Cat. Morphologic diagnosis: Spine, caudal, and overlying skin, spina bifida 12

13 46. Sheep. Pathogenesis: Ruminal acidosis= ingestion of readily fermentable feed proliferation of Gram + bacteria production of D-lactic acid reduction of ruminal ph (<5) plus increase in ruminal osmotic pressure die-off of Gram ( ) flora and release of endotoxin ruminal atony, mucosal damage increase in permeability of ruminal mucosa and vasculature third spacing of fluid and systemic endotoxin absorption shock 47. Squirrel monkey. Morphologic diagnosis: Oral cavity/tongue, tumor, squamous cell carcinoma Morris TH. A further case of squamous cell carcinoma in the oral cavity of a squirrel monkey. J Med Primatol Jul;23(5): PMID: Sasseville VG, Pauley DR, Spaulding GL, Chalifoux LV, Lee-Parritz D, Simon MA. A spontaneous squamous cell carcinoma of the oral cavity in a squirrel monkey (Saimiri sciureus). J Med Primatol Jun;22(4): PMID: Mouse 48. Mouse. Body type consistent with Myobia or Radfordia Single empodial (tarsal) claw = Myobia musculi 13

14 49. Zebrafish 49. Zebrafish. Morphologic diagnosis(es): Ultimobranchial gland, tumor, adenoma Heart/pericardium, pericardial edema/hydropericardium Bonus question: What sex is this zebrafish? Eosinophilic hepatocytes = male 50.Macaque. Morphologic diagnosis: Uterus (cervix), tumor, leiomyoma Bonus slide. Rat. Morphologic diagnosis(es): Thymus, tumor, eosinophilic granulocytic sarcoma (aka chloroma, extramedullary myeloblastoma) Myeloperoxidase content of the eosinophils imparts a green color to the neoplasm 14