THE NAME OF THE STAINS:
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1 THE NAME OF THE STAINS: HISTOLOGY HISTORY PART 2 Jean Mitchell, BS, HT (ASCP) Newcomer Supply Middleton, Wisconsin jmitchell@newcomersupply.com Jane Parr, BS, HT (ASCP) National Jewish Health & University of Colorado Anschutz Medical Center Denver, Colorado jane.parr@ucdenver.edu
2 THE NAMES OF THE STAINS
3 WHAT S MY NAME?
4 DR. ALOIS ALZHEIMER: German Neurologist : 51 Year-Old Woman w/psychological Problems Dementia : Brain Displayed Extensive Atrophy Sampled Thin Preps w/silver Salts 2 Types of Abnormal Nerve Cell Deposits Plaques & Tangles : Published Article of Observations Particular Disease Process of the Cerebral Cortex 5. Died from Heart & Kidney Problems
5 MAX BIELSCHOWSKY: German Neuropathologist : Senckenberg Path Institute Carl Weigert, Alois Alzheimer, Franz Nissl : Published Silver Technique Improvement on Cajal s Method : Kaiser Wilhelm Institute for Brain Research - Berlin : Escaped Nazi Germany Son: Franz Bielschowsky, Director of Cancer Genetics in Dunedin, New Zealand
6 BIELSCHOWSKY STAIN
7 WHAT S MY NAME?
8 GUSTAV GIEMSA German Chemist and Bacteriologist : Published Essay on Staining Procedure for Flagellates, Blood Cells & Bacteria Now Known as the Giemsa Stain 3. Designed for Demonstration of Parasites in Malaria 4. Oxidized Methylene Blue to Azure I
9 GIEMSA STAIN
10 WHAT S MY COUNTERSTAIN NAME?
11 IRA THOMPSON VAN GIESON: American Neurologist, Psychiatrist, Bacteriologist & Neuropathologist : Columbia Medical Degree Pathology Instructor of Nervous System 1889: Introduced the Picric Acid Stain : 1 st Director of Path Institute NY State Hospitals for the Insane : NY State Health Department 5. Practiced Hypnosis 6. Described as A Genius
12 VAN GIESON COUNTERSTAIN
13 WHAT S MY NAME?
14 FREDERICK HERMAN VERHOEFF: Eye Pathologist & Bacteriologist (from Louisville, KY) : Johns Hopkins Medical Degree : Mass Eye/Ear Institute Eye Surgeon, Consulting Chief, Professor : Howe Lab of Ophthalmology : Harvard Professor Ophthalmic Research & Pathology Published 100 s of Journal Articles : WWI Rank of Major
15 VERHOEFF ELASTIC STAIN
16 WHAT S MY NAME?
17 ALDRED SCOTT WARTHIN: Born in Indiana U of Michigan Med School 2. Director of Pathology U of Michigan Professor of Dieterlie & Starry 3. Studied Pathology of Syphilis (20 Years) : With Allen Starry ( ) Silver Nitrate Staining for Spirochetes 5. Father of Cancer Genetics 6. Author of Several Pathology Textbooks 7. Welleria aftonensis Warthin
18 WARTHIN-STARRY STAIN & WARTHIN TUMOR
19 ROBERT RICHARD DIETERLE: From Ann Arbor, Michigan 2. Neuro-Pathologist for U of Michigan, Department of Psychiatry 3. Guggenheim Fellow 4. Modified w/uranyl Nitrate Technique for Legionnaires Disease
20 GABRIEL STEINER: GRETE STEINER: ? 1. German/American Neurologist: Known for Multiple Sclerosis Research 2. Studied Syphilis & Spirochetes : Discovered Spirochetes in Brains of MS Patients : Immigrated to U.S. from Germany : Professor of Neurology/Neuropathology, Wayne State in Detroit : Director of Michigan Multiple Sclerosis Center : Relationship of Spirochetes Lyme Disease MS
21 WHAT S MY NAME?
22 HUGO SCHIFF: Born In Frankfurt Germany 2. Supporter of Socialism (Karl Marx) 1857: Bern, Switzerland 1863: Italy Pisa & Florence 3. Professor of General Chemistry Founder of Chemical Institute of Florence Hugo Schiff International Store House 4. Schiff Bases & Other Imines 5. Prolific Investigator: 300+ Publications
23 PERIODIC ACID SCHIFF (PAS) STAIN
24 WHAT S MY NAME?
25 CLAUDE PIERRE MASSON: Born in Dijon : Univ Paris & Pasteur Institute 3. Chair of Pathology: U of Strasbourg : U of Montreal One of the Best 20 th Century Path Schools : Some Histological Methods, Trichrome Staining & Their Preliminary Technique 1900: Mallory Introduced Trichrome 6. 1 st to Describe Neurocrine Secretion 7. Brain Tumor & Neurological Research
26 MASSON TRICHROME STAIN
27 WHAT S MY NAME?
28 NORMAN BARRETT: Australian Born British Thoracic Surgeon 2. Educated at Cambridge : Mayo Clinic on Rockefeller Fellowship Decision to Focus on Thoracic Surgery : Performed 1 st Successful Ruptured Esophagus Repair : Paper on Columnar vs Squamous Epithelium in the Esophagus Introduced Reflux Esophagious Columnar Epithelium Ascending Esophagus = Barrett s Esophagus
29 ALCIAN BLUE STAIN FOR BARRETT S ESOPHAGUS
30 WHAT S MY NAME?
31 GEORGE GOMORI: Hungarian-American Pathologist (Greek heritage) 2. University of Budapest: Pathology & Surgery : Immigrated to USA : U of Chicago, Pathologist & Professor : Founding Member of Histochemical Society : Microscopic Histochemistry: Principles & Practice 7. Phenomenal Intellect & Integrity 8. Died from Cardiac Arrest
32 STAINS DEVELOPED BY DR. GOMORI Gomori Silver Impregnation of Reticulin/Reticular Fibers Gomori One-Step Trichrome Gomori Prussian Blue Stain for Iron Gomori Methenamine Silver Stain Gomori Non-Specific Alkaline Phosphatase Stain Gomori-Wheatley Trichrome Stain: for Intestinal Protozoa
33 GOMORI STAINS
34 ROBERT G. GROCOTT: American Med Tech and Histologist (born in Beaver Falls, PA) Trained & Certified Histotechnologist : Chief of Histopathology Gorgas Hospital for Tropical Diseases U.S. Army Hospital in Panama Canal Zone : A Stain for Fungi in Tissue Sections and Smears Modification of Gomori s Stain (GMS Stain) 4. Book on Poisonous Snakes of Panama
35 WHAT S MY NAME?
36 DAVID BODIAN: American Medical Scientist (born in St. Louis) : Johns Hopkins University Worked in Polio & Neurological Research Structure & Action of Nerve Cells Ran an EM Lab s: Led the Groundwork for Polio Vaccine Development (Salk & Sabin) Polio Caused by 3 Distinct Viruses 4. Died from Parkinson s Disease
37 BODIAN STAIN FOR NERVE TISSUE
38 WHAT S MY PROCEDURAL NAME?
39 FREDERIC MOHS: Born in Wisconsin s: U of Wisconsin/Madison Med School : Research w/cancerous Rat Tissue : Chemosurgery on Human Skin Chemo Referred to Zinc Chloride Paste Paste Fixed the Skin (hours/day) Excise Tissue w/out Bleeding Mapped & Painted Edges Frozen Sections s: Procedure Skeptically Caught On s: Modified w/fresh Skin Tissue
40 MOHS PROCEDURE FOR SKIN CANCER
41 WHAT S MY NAME?
42 HOLDE PUCHTLER: Born in Germany : MD University of Koln 1955: Research Fellow - Montreal : U of Georgia Med School : 1 st International Congress of Histo/Cytochemistry Paris Histochemistry of Connective Tissue Fibers : On the Binding of CR by Amyloid 6. Bilingually Deaf: Lip Reading & Writing Assisted by Susan Meloan & Faye Sweat-Waldrop
43 PUCHTLER CONGO RED STAIN
44 Our Heartfelt Thanks To: Australian National Histology Conference NSH & Lee G Luna Foreign Travel Award
45 References Include: 1. Newcomer Supply for Stain Photo s: American Society of Microbiology: 5. American Journal of Clinical Pathology: 6. Royal Society of Chemistry: 7. Thomas Jefferson University: 8. Johns Hopkins University: 9. Journal of Histotechnology: Reference Articles A Short Histology of Histopathology Technique, Michael Titford, Vol 29, No 2, Alois Alzheimer, Venita Jay, Vol 25, No 2, 2002 Harris Hematoxylin, What Harris Really Wrote and the Mechanism of Hemalum Stain, Susan Meloan and Holde Puchtler, Vol 10, No 4, 1987.
The Oral Histology Series Series 5 Special Stains
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THE NAME OF THE STAINS:
THE NAME OF THE STAINS: HISTOLOGY HISTORY PART 1 Jean Mitchell, BS, HT (ASCP) Newcomer Supply Middleton, Wisconsin jmitchell@newcomersupply.com Jane Parr, BS, HT (ASCP) National Jewish Health & University
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