Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 Schedule of Accreditation 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Eye Pathology 1 st Floor, Cayton Street Building UCL Institute of Ophthalmology Bath Street London C1V 9EL United Kingdom Contact: Professor Philip Luthert Tel: +44 (0) p.luthert@ucl.ac.uk Website: Testing performed at the above address only DETAIL OF ACCREDITATION FLUIDS Ocular Pathology samples (eye lids, conjunctiva, cornea, lacrimal sac, lacrimal gland, orbit, intra-ocular structures, sclera / choroid, enucleations, eviscerations, exenterations) purposes of diagnosis Macroscopic and Microscopic examination In house documented procedures in conjunction with equipment as specified below: Specimen dissection Manual methods using documented in-house procedure PREP-003 Tissue Processing PREP 004 in conjunction with Leica ASP300S Processing Machine Tissue embedding PREP 005 in conjunction with Leica EG1150H Embedding Centre Tissue sectioning (microtomy) PREP 006 in conjunction with Leica RM2235 Microtome Assessment Manager: MP Page 1 of 7

2 Paraffin block tissues purposes of diagnosis Identification of basophilic and eosinophilic structures Staining: s for manual or automated staining using Leica XL Autostainer and the Haematoxylin and Eosin (STAIN 009) PAS positive substances Alcian Blue PAS (STAIN 002) s for manual staining using the Acid mucopolysaccharides Nuclear Counter stain Amyloid Mast cells Tissue components Gram +ve & -ve microogranisms Gram-negative bacteria Fungi Elastic fibres and connective tissue Hyaluronic acid and Chrondoitin sulphate Fibrin Melanin Pigment Granular Dystrophy Alcian Blue (STAIN 001) Celestine Blue Haematoxylin (STAIN 004) Congo Red Alkaline (STAIN 005) Giemsa (STAIN 006) Gomori's One Step Trichrome (STAIN 015) Gram (STAIN 034) Gram Twort (STAIN 006) Grocott Hexamine Silver (STAIN 008) Haematoxylin Van Gieson (STAIN 028) Hyaluronidase Digestion (STAIN 011) Martius Scarlet Blue (STAIN 012) Masson Fontana (STAIN 013) Masson Trichrome (STAIN 014) Assessment Manager: MP Page 2 of 7

3 Paraffin block tissues purposes of diagnosis (cont) Melanin pigment (by absence) Elastic tissue fibres, Muscle, Collagen Tubercle bacilli (TB) Simple lipids PAS positive substances PAS positive substances PAS positive substances Haemosiderin and ferric iron Reticulin Copper Copper-associated protein Mast cells Calcium Staining: s for manual or automated staining using Leica XL Autostainer and the Melanin Bleach (STAIN 016) Miller's Elastic Van Gieson (STAIN 029) Modified Ziehl Neelson (STAIN 033) Oil Red O (STAIN 017) Periodic Acid Schiff-Papanicolaou (STAIN 020) Periodic Acid Schiff (STAIN 021) Periodic Acid Schiff with Diastase (STAIN 022) Perls Prussian Blue (STAIN 023) Reticulin (STAIN 024) Rubeanic Acid (STAIN 025) Shikata s Orcein (STAIN 018) Toludine Blue (STAIN 027) Von Kossa (STAIN 030) Assessment Manager: MP Page 3 of 7

4 Acanthamoeba Epithelial cells Scattered T cells in germinal centres and mantle zone B cellsfollicular lymphoma Lymphoma Mesothelioma panel markerepithelial T cells in mantle zone T cells T and B cells-leukaemia T cells Childhood ALL, follicular lymphoma Reed Sternberg cells, hodgkins and meso panel B cells-lymphoma B cells- dendritic cells Lymphocytes in germinal centres Endothelial cells- malignancy ACANTHAMOEBA AE1/AE3 (MCK) BCL-2 BCL-6 BerEP-4 CD3 CD4 CD5 CD8 CD10 CD15 CD20 CD21 CD23 CD31 Assessment Manager: MP Page 4 of 7

5 : Vascular epithelium All white cells (LCA) Neuroectodermal cells, neurones, astrocytes Macrophages B cells B lymphocyte differentiation Epithelial malignancy Corneal cells Normal and neoplastic epithelia Cytokeratin low MWT Ductal and glandular epithelium Epithelial subtypes Neuroendocrine cells Mantle cell lymphoma Smooth and striated muscle Epithelial cells Glial cells Melanocytes CD34 CD45 CD56 CD68 CD79a CD138 CEA CK3 CK7 CK8/18 (CAM 5.2) CK19 CK20 CHROMOGRANIN A CYCLIN D1 DESMIN EMA GFAP HMB45 Assessment Manager: MP Page 5 of 7

6 : B cells Relation to IgG levels IgG expression IgG4 positive plasma cells Relation to IgG levels Light chains Proliferating cells Light chains Melanocytes Epithelial cells B cell lymphoma Muscle derived actin Peripheral nerves Squamous / Adeno / Small cell differentiation Retinal rods Smooth muscle Schwann cells, nerve processes, S100 +ve neoplasms Neuroendocrine cells HLA-DR IgD IgG IgG4 (or EP4420) IgM KAPPA KI-67 LAMBDA MEL A MNF116 MUM-1 M. ACTIN NEURO FILAMENT p63 RHODOPSIN SMA S100 SYNAPTOPHISIN Assessment Manager: MP Page 6 of 7

7 Vitreous fluids & corneal impression drum : Mesenchymal cells Endothelial cells and megakaryocytes Cytopathology: purposes of diagnosis Examination of slides to identify or VIMENTIN VON WILLIBRAND FACTOR (FACTOR VIII) Preparation of slides for staining: PREP015 in conjunction with Hettich Rotofix centrifuge Staining: s for manual staining using the Vitreous fluids Basic cellular components H&E and Cytopathology PAS Slides prepared in-house as above Morphological assessment and interpretation / diagnosis Microscopy and reporting using documented in-house procedure G001 END Assessment Manager: MP Page 7 of 7