3. Staining solutions for histology and cytology Mounting and embedding media... 60

Transcription

1

2 INDEX 1. Hematoxylin stains Eosin Staining solutions for histology and cytology Reagents I Reagents II Decalcifying solutions Fixatives Formalins Solvents Mounting and embedding media Legend... 61

3 Dear distributors, suppliers and customers, We are pleased to present our new catalog Microscopic stains and reagents for cytology, histology and microbiology Emmonya Biotech 3

4 1. Hematoxylin stains Products Code Size Description Gill s hematoxylin I ml For standard haematoxylin-eosin l staining of tissue sections. For nuclei. Gill s hematoxylin II ml For standard haematoxylin-eosin l staining of tissue sections. For nuclei. Gill s hematoxylin III ml For standard haematoxylin-eosin l staining of tissue sections. For nuclei Gill I Gill II Gill III 4

5 Mayer s hematoxylin ml For standard haematoxylin-eosin l staining of tissue sections. For nuclei Harris hematoxylin ml For standard haematoxylin-eosin l staining of tissue sections. For nuclei Weigert s Hematoxylin test For trichrome stains ml ml l Harris Mayer Weigert 5

6 2. Eosin Products Code Size Description Eosin Y 1% aqueous sol ml Standard haematoxylin-eosin staining ml for cytoplasm ml l Eosin Y 5% aqueous sol ml Standard haematoxylin-eosin staining ml for cytoplasm l Eosin Y 1% aqueous sol. Eosin Y 5% aqueous sol. 6

7 Eosin Y 0.5% alcoholic sol ml Standard haematoxylin-eosin staining ml for cytoplasm ml l Eosin-Phloxin B alcoholic sol ml Standard haematoxylin-eosin staining ml for cytoplasm ml l Eosin Y 0,5% alcoholic sol. Eosin-Phloxin B alcoholic sol. 7

8 3. Staining solutions for histology and cytology 8 Products Code Size Description Alcian blue ph test For demonstration of acid mucins in ml tissue sections ml l Alcian blue ph test For demonstration of carboxylated and ml some weakly sulfated mucins in tissue sections ml l Alcian blue ph1 - P.A.S test For neutral and acid mucins in tissue ml sections ml ml l Alcian blue ph2.5 - P.A.S test For neutral and acid mucins in tissue ml sections ml ml l

9 Alcian Yellow - Toluidine Blue test For screening patient specimens for ml Helicobacter pilory Azan Trichrome test Method for connective tissue, especially ml to show muscle fibers, collagen, reticulum, kidney glomerular stroma in l tissue sections Brilliant Cresyl Blue for reticulocytes test Blood smears examination for ml reticulocytes ml ml l Bennhold s Congo red for amyloid test Stain for amyloid deposits in tissues ml ml l Highman s Congo red for amyloid test Stain for amyloid deposits in tissues ml ml l CytoMax - fast Papanicolau for cyto PAP smear test For cyto PAP and other smears ml (cytological specimens) ml ml 9

10 Giemsa stain test Giemsa concentrate solution ml ml l Gomori silver impregnation test Method to show argyrophilic reticular fibers in connective tissue Gram for microbiology test Differentiation between Gram positive ml and Gram negative bacteria in different specimens ml ml l Grocott s methenamine silver test To identify fungi in tissue section nitrate method for fungi ml Haematocolor (hematocolor) test Blood smear examinations ml ml ml Leishman stain test Blood smear examinations ml ml ml 10

11 Leishman-Wright stain W 100test Blood smear examinations W W W 125ml 250ml 500ml Mallory trichrome test Staining solution for connective tissue Masson trichrome Goldner test Staining solution for connective tissue - collagen and muscle fiber Masson trichrome Standard test Staining solution for connective tissue - collagen and muscle fiber May Grunwald test Fixative for MGG blood smear ml examinations ml l May Grunwald - Giemsa test Blood smear examination l Methenamine Silver Impregnation (P.A.S.M.) test Kit for histology (lamina basalae, argyrophil cells etc.). Methylene Blue Loffler test Alkaline stain for cell nuclei in smears ml from different origin and pathogens ml l 11

12 New Methylene Blue for reticulocytes Oil Red O for lipids in frozen sections test Blood smear examination for ml reticulocytes ml test Lipid staining in frozen sections ml ml Orcein test To demonstrate elastic fibers in tissue ml sections, recomended for vascular pathology Papanicolau - OG ml Stain solution I for cyto PAP smears ml l Papanicolau - EA ml Stain solution II for cyto PAP smears ml l Papanicolau - EA ml Stain solution II for cyto PAP smears ml l Periodic Acid Schiff (P.A.S.) test Examination of mucosubstances ml (mucopolysaccharides), lamina basalae, glycogen deposits in liver etc ml in tissue sections ml 12

13 Rapihem test Blood smears, other cytological ml specimens and fine needle aspirations examination ml l Sudan III Propylene Glycol test Triglyceride staining in frozen sections ml ml l Sudan III alcohol solution A 100test Staining of triglycerides in frozen tissue A 250ml sections A 500ml A 1l Toluidine Blue stain for hystology test To demonstrate metachromatic ml substances and mast cells in tissue sections ml l Van Gieson Trichrome stain test Staining for connective tissue ml ml ml Weigert for elastic fibers test 125ml To demonstrate elastic fibers in tissue sections 13

14 Wright s Stain for cytology WP 100test Fine needle aspirations and other WP 125ml cytological specimens examination WP 250ml WP 500ml Ziehl Neelsen cold test Microscopic examination of ml Mycobacteria ml 14

15 15 ALCIAN BLUE ph 1 Alcian blue is used for the demonstration of acid mucins, which can be produced by several types of epithelial and connective tissue tumors. Acid mucins also play role in collagen diseases and early stages of atherosclerosis. procedure time 50 min. (20 min. microwave oven modification) storage conditions C, protected from fire and sunlight, tightly closed A Alcian blue staining solution рн 1 B Nuclear Fast Red staining solution Weakly and strongly sulfated acid mucins Blue Nuclei Red to pink Other tissue elements Pale pink Alcian blue ph1 reaction - intestine

16 16 ALCIAN BLUE ph 2,5 Alcian blue is used for the demonstration of acid mucins, which can be produced by several types of epithelial and connective tissue tumors. Acid mucins also play role in collagen diseases and early stages of atherosclerosis. procedure time ~50 min. (20 min. microwave oven modification) storage conditions C, protected from fire and sunlight, in glass dark bottle, tightly closed A Alcian blue staining solution рн 2,5 B Nuclear Fast Red staining solution Carboxylated and some weakly sulfated acid mucins Blue Nuclei Red to pink Other tissue elements Pale pink Alcian blue ph2,5 reaction - intestine

17 17 ALCIAN BLUE ph 1 - P.A.S. Alcian blue and PAS reactions can be combined to demonstrate both neutral and acid mucins in tissue sections. procedure time ~50 min. (20 min. microwave oven modification) storage conditions C, protected from fire and sunlight, tightly closed A Alcian blue staining solution рн 1 C Schiff Reagent B Periodic acid solution Weakly and strongly sulfated acid mucins Blue Neutral mucins Magenta Mixtures of acid and neutral mucins Bluish to reddish purple Nuclei (if contra-stained) Blue to black Alcian blue ph1-pas reaction - intestine

18 18 ALCIAN BLUE ph 2,5 - P.A.S. Alcian blue and PAS reactions can be combined to demonstrate both neutral and acid mucins in tissue sections. procedure time ~50 min. (20 min. microwave oven modification) storage conditions C, protected from fire and sunlight, in glass dark bottle, tightly closed A Alcian blue staining solution рн 2,5 C Schiff Reagent B Periodic acid solution Carboxylated and some faintly acid sulfomucins Blue Neutral mucins Magenta Mixtures of acid and neutral mucins Bluish to reddish purple Nuclei (if contra-stained) Blue to black Alcian blue ph2,5 reaction - intestine

19 19 ALCIAN YELLOW -TOLUIDINE BLUE Staining solution for screening patient specimens for Helicobacter pilory spiral shaped bacterium implicated in gastric inflammation, peptic ulcers and gastric cancer. procedure time ~25 min. storage conditions C, protected from fire and sunlight, tightly closed А Periodic acid С Alcian yellow staining solution Е Sodium hydroxide solution В Sodium bisulfite solution D Toluidine blue staining solution Helicobacter pilory Dark blue Mucins Yellow Background Blue scale Alcian yellow-toluidine blue reaction - intestine

20 20 AZAN TRICHROME Recommended method for connective tissue, especially to show muscle fibers, collagen, reticulum, kidney glomerular stroma in tissue sections. procedure time ~100 min. storage conditions C, protected from fire and sunlight, tightly closed А Azocarmine solution C Differentiation solution E Staining solution В Aniline solution D Phosphomolybdic acid solution Collagen, reticulum, and kidney glomerular stroma Deep blue Muscle Orange Erythrocytes Red A B Azan trichrome staining: A) kidney; B)uterus

21 21 BRILLIANT CRESYL BLUE Blood smears examination for reticulocytes. Brilliant cresyl blue is more used in hematology laboratories than in histology laboratories. It is invaluable for the staining of reticulocytes whereas its use in histology is limited to vital staining. procedure time ~35 min. storage conditions C, protected from fire and sunlight, tightly closed Brilliant Cresyl Blue physiological solution Reticulocytes Greenish to blue with dark blue ribonucleoprotein granules inside Erythrocytes Greenish to blue Brilliant Cresyl Blue - blood smear

22 22 BENNHOLD S CONGO RED Stain for amyloid deposits in tissues. Appropriate for tissue sections from 5-12μm. Amyloid deposits are formed in tissues spontaneously or in association with a wide range of diseases. In cases of suspected amyloidosis, thick sections at 12 μm may be beneficial in demonstrating minute deposits of amyloid. procedure time ~90 min. storage conditions C, protected from fire and sunlight, tightly closed A Congo red staining solution according Benhold C Mayer s haematoxylin solution B Sodium hydroxide solution Amyloid Pink to red (apple-green birefringence with polarized light) Nuclei Blue Bennhold s congo red for amyloid - kidney

23 23 HIGHMAN S CONGO RED Stain for amyloid deposits in tissues. Suitable for paraffin sections fixed in 10% formalin. Amyloid deposits are formed in tissues spontaneously or in association with a wide range of diseases. In cases of suspected amyloidosis, thick sections at 12 μm may be beneficial in demonstrating minute deposits of amyloid. procedure time ~20 min. storage conditions C, protected from fire and sunlight, tightly closed A- Congo red staining solution according Highman B- Potassium hydroxide solution Amyloid Pink to red (apple-green birefringence with polarized light) Nuclei Blue if contra-stained Highman s congo red for amyloid - kindey

24 24 CYTOMAX Stain solution for cyto PAP and other smears (cytological specimens). procedure time 3 min. storage conditions C, protected from fire and sunlight, tightly closed А Harris`s haematoxylin solution C Modified polychromic EA65 solution B 2-Propanol Absolute D 80% 2-Propanol Nuclei Violet-blue to black Cytoplasm (eosinophilic) Pink Cytoplasm (basophilic) Turquoise to blue Cytoplasm (metaplasia cells) With both blue and pink Eritrocytes Red Microorganisms Blue-violet Cytomax - cyto PAP smear

25 25 GIEMSA STAIN Blood smears examination. procedure time ~40 min. storage conditions C, protected from fire and sunlight, tightly closed Giemsa concentrate solution Lymphocytes Light blue cytoplasm Monocytes U shaped nucleus Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (with dark violet to black granules) Platelets Violet Eritrocytes Light red Blood parasites Red Giemsa stain - blood smear

26 26 GOMORI SILVER IMPREGNATION Recommended method to show argyrophilic reticular fibers in connective tissue, especially to differentiate collagen fibers from connectivum. procedure time ~30 min. product validity 1 year (under recomended conditions) storage conditions C, protected from fire and sunlight, in glass dark bottle, tightly closed А Potassium permanganate solution С Oxalic acid solution E Ammoniacal silver solution G Sodium hyposulphite solution В Acid activating buffer D Ferric ammonium sulfate solution F Neutral formalin solution Reticular fibers and nervous cells Black Connective tissue Tobacco brown Collagen Gold yellow Gomori silver impregnation - kidney

27 27 GRAM Differentiation between Gram positive and Gram negative bacteria in different specimens. procedure time 5 min. storage conditions C, protected from fire and sunlight, tightly closed A Crystal Violet solution C Decolorising solution B Lugol s solution for Gram D Safranin O alcoholic solution Gram positive microorganisms Blue Gram negative microorganisms Red Gram staining - bacterial speciments

28 28 GROCOTT for fungi To identify fungi in tissue sections. The mucopolysaccharide components of the fungal cell wall are oxidized to release aldehyde groups. The aldehyde groups then react with the silver nitrate, reducing it to a metallic silver, rendering them visible. procedure time ~100 min. (~25 min. microwave oven modification) product validity 1,5 years (under recomended conditions) storage conditions 4 C, protected from fire and sunlight, tightly closed А Chromic acid solution С Methenamine solution E Borax water solution G Sodium thiosulfate solution В Sodium hydrogensulfite solution D Silver nitrate solution F Gold chloride water solution H Light Green solution Fungi Black Mucin Grey Background Green Grocott s method for fungi - gastric tissue

29 29 HAEMATOCOLOR (HEMATOCOLOR) Blood smears examination. procedure time 1 min. storage conditions C, protected from fire and sunlight, tightly closed A Methanol absolute C Haematocolor Solution II B Haematocolor Solution I D Haematocolor Phosphate buffer Lymphocytes Bluish Monocytes U shaped nucleus Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine granules) Platelets Purple Eritrocytes Purple-pinkish Haematocolor - blood smear

30 30 LEISHMAN STAIN Blood smears examination. procedure time 7 min. storage conditions C, protected from fire and sunlight, tightly closed A Leishman staining solution B Buffered water Lymphocytes Bluish Monocytes U shaped nucleus Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine granules) Platelets Pink-violet Eritrocytes Pink Leishman stain - blood smear

31 31 LEISHMAN-WRIGHT STAIN Blood smears examination. procedure time 7 min. storage conditions C, protected from fire and sunlight, tightly closed A Leishman-Wright staining solution B Buffered water Lymphocytes Bluish Monocytes U shaped nucleus Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine granules) Platelets Pink-violet Eritrocytes Pink Leishman-Wright stain - blood smear

32 32 MALLORY TRICHROME Staining solution for connective tissue. procedure time ~20 min. storage conditions C, protected from fire and sunlight, tightly closed А Acid fuchsin staining solution C Mallory s staining solution B Phosphomolybdic acid solution Nuclei Red Erythrocytes Orange red Muscle Red Collagen Blue Mallory trichrome - placenta

33 33 MASSON TRICHROME STANDARD Staining solution for histology. Collagen and muscle fiber stain differentially when treated sequentially with Acid Fuchsin, PTA/PMA and Aniline Blue. procedure time ~40 min (+/- pre-fixation time). storage conditions C, protected from fire and sunlight, tightly closed A- Weigert s Haematoxylin solution A C- Acid fuchsin staining solution E- Phosphotungstic acid solution G- Acetic acid solution B- Weigert s Haematoxylin solution B D- Phosphomolybdic acid solution F- Masson s aniline blue solution Nuclei Black Muscle fibers, cytoplasm, erythrocytes Red Collagen, mucus Blue Masson trichrome standard - uterus

34 34 MASSON TRICHROME GOLDNER Staining solution for histology. Collagen and muscle fiber stain differentially when treated sequentially with Acid Fuchsin, PTA and Light green. procedure time ~40 min. (+/- pre-fixation time). storage conditions C, protected from fire and sunlight, tightly closed A- Weigert s Haematoxylin solution A C- Acid fuchsin staining solution E- Light green staining solution B- Weigert s Haematoxylin solution B D- Phosphotungstic acid solution Nuclei Black Muscle fibers, cytoplasm, erythrocytes Red Collagen Greenish Masson trichrome Goldner - uterus

35 35 MAY GRUNWALD STAIN Supplementary stain for May Grunwald Giemsa blood smear examination. procedure time ~2 min. (+ Giemsa co-staining) storage conditions C, protected from fire and sunlight, tightly closed May - Grunwald Stain solution White blood cells Faintly shaped Erythrocytes Pinkish-red May Grunwald stain only May Grunwald - Giemsa May Grunwald stain - blood smear

36 36 MAY GRUNWALD - GIEMSA Blood smear examination and cytodiagnosis. procedure time ~10-35 min. storage conditions C, protected from fire and sunlight, tightly closed A Methanol absolute C Giemsa staining solution B May-Grunwald staining solution D Sorenson buffer ph7 Lymphocytes Light blue cytoplasm Monocytes U shaped nucleus Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (with dark violet to black granules) Platelets Violet Eritrocytes Reddish brown Blood parasites Red May Grunwald Giemsa - blood smear

37 37 METHENAMINE SILVER IMPREGNATION - P.A.S.M. Kit for histology (lamina basalae, argyrophil cells etc.). procedure time ~2 h. product validity 1 year (under recomended conditions) storage conditions 4 C, in dark glass bottle, protected from fire and sunlight, tightly closed А Periodic acid solution C Hexamethylene tetraamine solution E Gold chloride solution В Silver nitrate solution D Sodium tetraborate solution F Fixative Lamina basalae Black Nuclei and background Pinkish-red and green if contra-stained Positive control Skin, kydney P.A.S.M. - kidney

38 38 METHYLENE BLUE LOFFLER Alkaline stain for cell nuclei in smears from different origin. Pathogens (bacteria) examination. procedure time ~5 min. storage conditions C, protected from fire and sunlight, tightly closed Methylene Blue solution according Loffler Bacteria Blue Leucocytes nuclei Blue Epithelial cells cytoplasm Slightly blue Epithelial cells nuclei Blue A B Methylene Blue Loffler: A) mouth epithel; B)blood smear

39 39 NEW METHYLENE BLUE Blood smears examination for reticulocytes. Reticulocytes are juvenile red cells, which contain remnants of basophilic ribonucleoproteins. This material reacts with the dye to form a bluish black precipitate of granules or filaments. procedure time ~20 min. storage conditions C, protected from fire and sunlight, tightly closed New Methylene Blue water, isotonic solution, tilled with anticoagulant Reticulocytes Greenish to blue with dark blue ribonucleoprotein granules inside Erythrocytes Greenish to blue New Methylene Blue - blood smear

40 40 OIL RED O Lipid staining in frozen sections. procedure time ~40 min. storage conditions C, protected from fire and sunlight, tightly closed A - Propylene glycol absolute C - Propylene glycol 85% water solution B Staining solution Oil red O D Mayer s haematoxylin solution Nuclei Blue to black Lipids Red Oil red O - omentum cryostate section

41 41 ORCEIN for elastic fibers To demonstrate elastic fibers in tissue sections; recommended for vascular pathology. procedure time ~30 min storage conditions C, protected from fire and sunlight, tightly closed А Potassium permanganate solution С Oxalic acid solution Е Orcein solution according Shikata В Acid activation buffer D Incubation media F Differentiation solution Elastic fibers Purplish red-brown Background Almost colourless A B Orcein for elastic fibers: A) blood vessels; B) kidney

42 42 PAPANICOLAU Stain solution for cyto PAP smears. N.B! EA65 (Emmonya Biotech Ltd.) is an alternative for EA50 stain, providing more intensive cytoplasm staining! procedure time ~25 min storage conditions C, protected from fire and sunlight, tightly closed А Harris`s haematoxylin solution C EA50/65 polychromic stain solution В Orange G-6 stain solution Nuclei Orange-brown to bluish-black Cytoplasm (keratinized) Orange-pink Cytoplasm (basophilic) Blue-green (EA50) to turquoise (EA65) Cytoplasm (metaplasia cells) With both blue and pink Cytoplasm (eosinophilic) Pink Eritrocytes Red Microorganisms Blue-violet to blue-black A B Papanicolau for cyto PAP smears: A) EA 50; B)EA 65

43 43 PERIODIC ACID SCHIFF (P.A.S.) Examination of mucosubstances (mucopolysaccharides), lamina basalae, glycogen deposits in liver etc. in tissue sections. procedure time ~35 min. storage conditions C,in dark glass bottle, protected from fire and sunlight, tightly closed A Periodic acid solution C Mayer s haematoxylin solutionn B Schiff Reagent Glycogen, fungi, neutral mucosubstances, and some lamina basalae Magenta Nuclei Blue PAS reaction - gastric tissue (stomach)

44 44 RAPIHEM Blood smears, other cytological specimens and fine needle aspirations examination. Recommendable for fast blood smears examination! procedure time ~ 40 sec. storage conditions C, protected from fire and sunlight, tightly closed A Rapihem fixative С Rapihem solution II В Rapihem solution I D Rapihem washing solution - concentrate Cells Clear shaped morphology (nucleus, cytoplasm), due to specimen origin! Enabling interpretation of their methabolitic condition A B Rapihem : A) Fine needle aspiration; B) Blood smear

45 45 SUDAN III - alcohol solution Staining of triglycerides in frozen tissue sections. procedure time ~35 min. storage conditions C, protected from fire and sunlight, tightly closed A 50% Ethanol C Mayer s haematoxylin solution B Staining solution Sudan III (alcoholic solution) Nuclei Blue to black Lipids Orange Sudan III alcohol solution - omentum cryostate section

46 46 SUDAN III - Propylene Glycol Triglycerides staining in frozen sections. procedure time ~25 min. storage conditions C, protected from fire and sunlight, tightly closed A Staining solution Sudan III C - 50% Propylene glycol water solution B - 85% Propylene glycol water solution D Mayer s haematoxylin solution Nuclei Blue to black Lipids Orange Sudan III Propylene Glycol - omentum cryostate section

47 47 TOLUIDINE BLUE 1. To demonstrate mucopolysaccharides and other metachromatic substances in tissue sections. 2. To demonstrate mast cells (with histamine, heparin metachromatic granules) in tissue sections. procedure time ~6 min. storage conditions C, protected from fire and sunlight, tightly closed Toluidine blue solution Tissue structure Visible like in standard Haematoxylin/ Eosin histology staining, but in blue scale. Mast cells Violet metachromatic staining Positive control Skin A B Toluidine Blue staining: A) skin; B) uterus

48 48 VAN GIESON TRICHROME Staining of connective tissue. procedure time ~35 min. storage conditions C, protected from fire and sunlight, tightly closed A Weigert s Haematoxylin solution B C Van Gieson s picrofuchsin B Weigert s Haematoxylin solution B Nuclei Black Collagen fibers Red Muscle, cornified epithelium, erythrocytes Yellow A B Van Gieson trichrome staining: A) skin; B) placenta

49 49 WEIGERT for elastic fibers To demonstrate elastic fibers in tissue sections; recommended for vascular pathology. procedure time ~50 min. storage conditions C, protected from fire and sunlight, tightly closed А Periodic acid solution С Weigert s resorcin fuchsin soution Е Carmalum according to Mayer solution В - Alcoholic reagent for incubation box D Acid differentiation buffer Elastic fibers Purplish red-brown Nuclei Red Weigert for elastic fibers - kidney

50 50 WRIGHT S STAIN for cytology Fine needle aspirations and other cytological specimens examination procedure time ~2 min storage conditions C, protected from fire and sunlight, tightly closed A Methanol absolute С Concentrated Phosphate buffer рн 6,8 В Wrght s staining solution Cells Clear shaped morphology (nucleus, cytoplasm), due to specimen origin! Enabling interpretation of their methabolitic condition Erythocytes Pinkish-green Wright s Stain for cytology - fine needle aspiration

51 51 COLD ZIEHL-NEELSEN Microscopic examination of Mycobacteria. Higher concentration of basic fuchsin and phenol in Kinyoun s carbol fuchsin solution helps penetration of Mycobacteria s cell wall and removes the need for pre-heating the slides. procedure time ~45 min. storage conditions C, protected from fire and sunlight, tightly closed А Kinyoun s carbol fuchsin solution С Methylene blue (water) staining solution В Acid alcohol solution Tubercle bacilli Bright red Background Light blue Ziehl-Neelsen stain - sputum

52 4. Reagents I 52 Method Reagent Stability ALCIAN BLUE ph 1- PAS A Alcian blue staining solution рн 1 2 B Periodic acid solution 2 C Schiff Reagent 2 ALCIAN BLUE ph 2.5 PAS A Alcian blue staining solution рн 2,5 2 B Periodic acid solution 2 C Schiff Reagent 2 ALCIAN BLUE ph 2.5 A Alcian blue staining solution рн 2,5 2 B Nuclear Fast Red staining solution 2 ALCIAN BLUE ph 1 A Alcian blue staining solution рн 1 2 B Nuclear Fast Red staining solution 2 ALCIAN YELLOW А Periodic acid solution 2 TOLUIDINE BLUE В Sodium bisulfite solution 2 С Alcian yellow staining solution 2 D Toluidine blue staining solution 2 Е Sodium hydroxide solution 2 AZAN TRICHROMЕ А Azocarmine solution 2 В Aniline solution 2 C Differentiation solution 2

53 D Phosphomolybdic acid solution 2 E Staining solution 2 BRILLIANT CRESYL BLUE Brilliant Cresyl Blue physiological solution 2 BENNHOLD S CONGO RED A Congo red staining solution according 2 Benhold B Sodium hydroxide solution 2 C Mayer s haematoxylin 2 HIGHMAN S CONGO RED A- Congo red staining solution according 2 Highman B- Potassium hydroxide solution 2 CYTOMAX А Harris`s haematoxylin solution 2 B 2-Propanol Absolute 2 C Modified polychromic EA65 solution 2 D 80% 2-Propanol 2 EOSIN Eosin Y 1% water solution 2 ACQUEOUS SOLUTION 1% EOSIN Eosin Y 5% water solution 2 ACQUEOUS SOLUTION 5% EOSIN Eosin Y 0.5% alcoholic solution 2 ALCOHOLIC SOLUTION 0.5% EOSIN-PHLOXINE А Mayer s haematoxylin 2 ALCOHOLIC SOLUTION В Scott s solution (water) 2 С Eosin-Phloxine staining solution 2 GIEMSA STAIN Giemsa concentrate solution 2 GILL S I HAEMATOXYLIN Gill s 1 Haematoxylin solution 2 53

54 GILL S II HAEMATOXYLIN Gill s 2 Haematoxylin solution 2 GILL S III HAEMATOXYLIN Gill s 3 Haematoxylin solution 2 GOMORI SILVER А Potassium permanganate solution 2 IMPREGNATION В Acid activating buffer 2 С Oxalic acid solution 2 D Ferric ammonium sulfate solution (alum) 2 E Ammoniacal silver solution 1 F Neutral formalin solution 2 G Sodium hyposulphite solution 2 GRAM STAIN A Crystal Violet solution 2 B Lugol s solution for Gram 2 C Decolorising solution 2 D Safranin O alcoholic solution 2 GROCOTT S А Chromic acid solution 2 В Sodium hydrogensulfite solution 2 С Methenamine solution 2 D Silver nitrate solution 2 E Borax water solution 2 F Gold chloride water solution 2 G Sodium thiosulfate solution 2 H Light Green solution 2 HAEMATOCOLOR A Methanol absolute 2 B Haematocolor solution I 2 54

55 C Haematocolor solution II 2 D - Haematocolor Phosphate buffer 2 HARRIS S HAEMATOXYLIN Harris s haematoxylin solution 2 LEISHMAN STAIN A Leishman Staining solution 2 B Buffered water 2 LEISHMAN- WRIGHT STAIN A Leishman-Wright staining solution 2 B Buffered water 2 MALLORY TRICHROMЕ А Acid fuchsin staining solution 2 B Phosphomolybdic acid solution 2 C Mallory s staining solution 2 MASSON TRICHROME A- Weigert s Haematoxylin solution A 2 GOLDNER B- Weigert s Haematoxylin solution B 2 C- Acid fuchsin staining solution 2 D- Phosphotungstic acid solution 2 E- Light green staining solution 2 MASSON TRICHROME A- Weigert s Haematoxylin solution A 2 STANDARD B- Weigert s Haematoxylin solution B 2 C- Acid fuchsin staining solution 2 D- Phosphomolybdic acid solution 2 E- Phosphotungstic acid solution 2 F- Masson s aniline blue solution 2 G- Acetic acid solution 2 MAY GRÜNWALD - GIEMSA A Methanol absolute 2 55

56 56 B May-Grunwald staining solution 2 C Giemsa staining solution 2 D Sorenson (Sorensen) buffer ph7 2 MAYER S HAEMATOXYLIN Мayer s Haematoxylin solution 2 MAY - GRUNWALD STAIN May - Grunwald Stain solution 2 METHENAMINE А Periodic acid solution 2 SILVER IMPREGNATION В Silver nitrate solution 1 P.A.S.M C Hexamethylene tetraamine solution 1 D Sodium tetraborate solution 2 E Gold chloride solution 1 F Fixative 2 METHYLENE BLUE LӦFFLER Methylene Blue solution according Loffler NEW METHYLENE BLUE New Methylene Blue water, isotonic solution, tilled with anticoagulant OIL RED O A Propylene glycol absolute B Staining solution Oil red O C Propylene glycol 85% water solution D Mayer s haematoxylin solution ORCEIN А Potassium permanganate solution В Acid activation buffer С Oxalic acid solution D Incubation media Е Orcein solution according Shikata F Differentiation solution

57 PAPANICOLAU PERIODOC ACID SCHIFF (PAS) RAPIHEM SUDAN III PROPYLENE GLYCOL SUDAN III ALCOHOL SOLUTION TOLUIDINE BLUE STAIN VAN GIESON TRICHROME STAIN WEIGERT for elastic fibers А Harris`s haematoxylin solution В Orange G-6 stain solution C EA50 polychromic stain solution C EA65 polychromic stain solution A Periodic acid solution B Schiff Reagent C Mayer s haematoxylin solution A Rapihem fixative В Rapihem solution I С Rapihem solution II D Rapihem washing solution - concentrate A Staining solution Sudan III B 85% Propylene glycol water solution C - 50% Propylene glycol water solution D Mayer s haematoxylin solution A 50% Ethanol B Staining solution Sudan III (alcoholic solution) C Mayer s haematoxylin solution Toluidine blue solution A Weigert s Haematoxylin solution A B Weigert s Haematoxylin solution B C Van Gieson s picrofuchsin. А Periodic acid solution 57

58 WEIGERT S HAEMATOXYLIN WRIGHT S STAIN for cytology COLD ZIEHL-NEELSEN В - Alcoholic reagent for incubation box С Weigert's resorcin fuchsin D Acid differentiation buffer Е Carmalum according to Mayer A Weigert s Haematoxylin solution A B Weigert s Haematoxylin solution B A Methanol absolute В Wrght s staining solution С Concentrated Phosphate buffer рн 6,8 A - Kinyoun s carbol-fuchsin solution B - Acetic alcohol solution C - Methylene Blue solution 58

59 4. Reagents II Products Code Size Description Acid ethanol Shikata E 125ml Differentiating agent E 250ml E 500ml Acid ethanol Weigert C 125ml Differentiating agent C 250ml C 500ml C 1l Hidrocloric Acid (1M) ml Differenting agent ml ml l Kovach s indole reagent ml For identification of indole ml ml l Orcein Acetic ml For chromosomal staining 59

60 Oxalic Acid ml Decolorant ml ml l Phosphate buffer ph ml ml ml l Phosphate buffer ph D 125ml D 250ml D 500ml D 1l Picric Acid aqueous sol. 1.2% ml For fixatives mixtures ml ml l Picric Acid alcoholic sol ml For erythrocytes in trichrome staining ml ml l Picrofuchsin ml For cytoplasm, muscle and collagen 60

61 Potassium Methabisulfite 0.5% ml Differentiating agent ml ml l Sodium Thiosulphate 5% ml Decolorant after treatment with lugol ml solution ml l Sulphuric Acid 0.5% ml For oxidation with permanganate ml ml 61

62 6. Decalcifying solutions Product Supplementary Code Description DEK HL DEK-HL decalcifying solution For fibrillar and Acid solution for DEK-HL decalcifying solution lamellar bone structure Ammonium solution for DEK-HL decalcifying solution DEK HN DEK-HN decalcifying solution For bone tissue Sodium solution for DEK-HN decalcifying solution DEK MF DEK-MF decalcifying solution Universal 7. Fixatives for histology Products Size Code Description Bouin 500ml Picric acid/formalin 1000ml fixative 2500ml Saccomano 25ml Fixative for cytology 1000ml speciments 62

63 8. Formalins Products Size Code Description Formaline 38-40% 1L L Formaline 30% w/v 5L Formalin 10% solution ph buffered 1L L L Solvents Products Size Code Description Bio Clear 1L L Prohyol 95 1L L Prohyol absolute 1L L

64 10. Mounting and embed media Products Size Code Description Paraffin wax histology C 1kg Lepka 100ml Eurokit 100ml Canada balsam 100ml

65 11. Legend Skin corrosion, categories 1A, 1B, 1C Serious eye damage, category 1 Acute toxicity (oral, dermal, inhalation), category 4 Skin irritation, categories 2, 3 Eye irritation, category 2A Skin sensitization, category 1 Specific target organ toxicity following single exposure, category 3 o Respiratory tract irritation o Narcotic effects Acute toxicity (oral, dermal, inhalation), categories 1, 2, 3 Flammable gases, category 1 Flammable aerosols, categories 1, 2 Flammable liquids, categories 1, 2, 3 Flammable solids, categories 1, 2 65

66