Supplemental Figure 1

Transcription

1 A1 A A5 A7 A9 A A4 A6 A8 A10 A1/A Gleason Score +=6 A/A4 Gleason Score +=6 Small glands with well defined Prominent feature of size variation lumen of various size infiltrating in to and distinct lumina favors Gleason adjacent prostate tissue pattern. Some tangentially characteristic of Gleason pattern sectioned glands appearing as small, (arrow). Glands are seen dispersed solid nests are noted in section A with in the connective tissue of (arrow) gland A5/A6 Gleason Score +=6 Distinct glandular units with moderate size lumina. Size variation is less marked. Invasion in to normal glandular Connective tissue is seen in section A5 (Arrow). A7/A8 Gleason Score 4+=7 Predominant pattern is type 4 as glands are angulated, fused and cells in ribbon pattern are also seen. Well formed glands of Pattern are also visible in section A8 (star). Fused glands, including the "hypernephromatoid" pattern, were defined by Gleason as pattern 4. A9/A10 Gleason Score4+4=8 Predominantly irregular cribriform and fused glands (pattern 4, Black arrow) admixed with very few well defined smaller glands of pattern (Yellow arrow). However large majority of sections has pattern 4 involving more than 99% area, score of 4+4 was given to these specimen. Supplemental Figure 1

2 B1 B B5 B7 B9 B B4 B6 B8 B10 B1/B Gleason Score +=6 prominent feature. Some tangentially sectioned glands appearing as small, solid nests are noted in section B1 (arrow) B/B4 Gleason Score +=5 Less variable shape, large sized and closely packed glands with well formed lumina are characteristic of Gleason pattern glands (star). Gleason pattern type glands seen in section B4 (40X) are separated from each other and relatively smaller in size compare to type glands in section B. However pattern was most predominant among all section to score +. B5/B6 Gleason Score +=6 with extension into adjacent non-neoplastic classification of gleason pattern. Variation in shape and size is a prominent feature. Blood Vessel (arrow). Although the cytoplasmic description is not the part of Gleason grading basophilic cytoplasm is very distinct here (Red Arrow). B7/B8 Gleason Score 5+5=10 Tumor cells are seen in solid sheets and cords without any organized lumina. Poorly differentiated cancer cells with out any glandular architecture is a typical Gleason pattern 5. Most predominant pattern of 5 gives gleason score of 5+5. B9/B10 Gleason Score +=5 Small glands are infiltrating in to the connective tissue of normal gland of Gleason type (arrow) mixed with large sized and closely packed glands of pattern are seen in section B10 gives score of +.

3 C1 C C5 C7 C9 C C4 C6 C8 C10 C1/C Gleason Score 5+5=10 Malignant cells in clusters without any attempt of gland formation. Cells are seen as cords or sheets. Predominant pattern in both sections is gleason 5 with score of 5+5. C/C4 Gleason Score +=5 Glands with variation in size and shape invading normal prostate tissue favoring classification of Gleason in section C(Arrow). Glands with lesser size variation and glands which are closely packed favors Gleason pattern (star). Predominant pattern in both the sections was however gleason resulting score of + C5/C6 Gleason Score +=6 prominent feature. Blood Vessel (arrow). Although the cytoplasmic description is not the part of Gleason grading basophilic cytoplasm is very distinct here (Red Arrow). C7/C8 Gleason Score +=5 Small glands are infiltrating in to the connective tissue of normal gland. Variation in size is very clear feature (arrow). Although pattern glands are most prominent; well formed large sized and closely packed glands of pattern are seen in section C8 (star) gives score of +. C9/C10 Gleason Score +=6 prominent feature. Cribriform glands of pattern are seen in section C10 (arrow)

4 D1 D D5 D7 D9 D D4 D6 D8 D10 D1/D Gleason Score 5+5=10 Malignant cells in clusters without any attempt of gland formation. Cells are seen as cords or sheets. Predominant pattern in both sections is gleason 5 with score of 5+5. Blood vessels are seen in section D1(arrow). D/D4 Gleason Score +=6 prominent feature (arrow) tangentially sectioned glands appearing as small, solid nests are noted in section D( Red Arrow) D5/D6 Gleason Score +=6 prominent feature. D7/D8 Gleason Score +=6 Prominent feature of size variation and distinct lumina favors Gleason pattern. Although an overlapping pattern towards gleason or 4 is very difficult to separate. Smaller glands with size variation of lumina favors pattern over pattern (star). Cribriform pattern can be confused with pattern 4 but well defined lumina seen in sectiond7 (arrow) favors pattern over 4. D9/D10 Gleason Score +=6 prominent feature. Fused, poorly formed and angulated glands of pattern 4(arrow) was second predominant feature favoring score +4

5 E1 E E5 E7 E9 E E4 E6 E8 E10 E1/E Gleason Score 5+5=10 Poorly differentiated malignant cells arranged in sheets and cords Very few poorly formed, fused and irregular glands of pattern 4 are also seen (arrow). As More than 99% pattern was gleason 5 resulting score of 5+5. Blood vessels ( Red arrow) E/E4 Gleason Score +=6 prominent feature (arrow) tangentially sectioned glands appearing as small, solid nests are noted in section E( Red Arrow) E5/E6 Gleason Score 4+4=8 Poorly formed, fused and irregular glands of pattern 4 are prominent feature of the specimen. As More than 99% pattern was gleason 4, resulting score is4+4. Typical cribriform pattern 4 is seen in section E5. E7/E8 Gleason pattern +=6 Mixture of large hyperplastic glands with smaller infiltrating glands of gleason pattern (arrow). As most predominant pattern was gleason Gleason score of +was given to the tissue specimen. E1/E Gleason Score 5+5=10 Poorly differentiated malignant cells arranged in sheets and cords. As More than 99% pattern was gleason 5 resulting score of 5+5. Blood vessels (arrow).

6 A Seq # b y D R T C N V T S H G I D G C D L L C C G R D Peptide K.ATRESAFVHAIASAGVAFAVTR.S K.CHGLSGSCEVK.T K.CHGLSGSCEVK.T R.CVFHWCCYVSCQECTR.V R.DLVYYEASPNFCEPNPETGSFGTR.D R.DRTCNVSSHGIDGCDLLCCGR.G R.DRTCNVSSHGIDGCDLLCCGR.G Supplemental Figure Spot# Scan# + MH+ MH XCorr dcn C (-0.0) E (+0.0) C (+0.) F (+0.5) E (+0.1) E (+1.0) D (+0.9) R.DRTCNVSSHGIDGCDLLCCGR.G EE (-0.1) R.EFADARENRPDAR.S AA (+0.) R.EFADARENRPDAR.S D (-1.0) R.EFADARENRPDAR.S E (-0.8) B SW:WNA_HUMAN wnt-a protein precursor. R.EKCR.C R.EKCR.C R.ENRPDARSAMNR.H A8 C6 D (+0.) (+0.4) (-0.4) * 0.0* MAPLGYFLLL CSLKQALGSY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT CK R.ENRPDARSAMNR.H R.ENRPDARSAMNR.H R.ESAFVHAIASAGVAFAVTR.S R.ESAFVHAIASAGVAFAVTR.S R.ESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSR.H R.ESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSR.H D10 E6 E10 C7 D4 C (-0.1) (+0.9) (+0.1) (+0.) C Coverage: AA 5.8% (186 / 5 residues) Mass 5.% (0580 / 965 Da) R.GWVETLRPR.Y R.GWVETLRPR.Y R.GWVETLRPR.Y R.GWVETLRPRYTYFK.V R.HQGSPGKGWK.W EE10 A8 F B7 B (-0.) (-0.) (-0.0) (-0.6) (-0.9) Relative Abundance K.QLRFCR.N R.SCAEGTAAICGCSSR.H R.TCNVSSHGIDGCDLLCCGR.G R.TCNVSSHGIDGCDLLCCGR.G R.TCNVSSHGIDGCDLLCCGR.G K.WGGCSEDIEFGGMVSR.E K.WGGCSEDIEFGGMVSR.E E9 E10 F E8 F AA7 B (+0.) (+0.6) (-0.0) (-0.) (-0.8) K.WGGCSEDIEFGGMVSREFADAR.E E (-0.9) R.WNCTTVHDSLAIFGPVLDK.A A (+0.5) Cancer Grades K.YDSASEMVVEK.H F (+0.) Control Low Medium High

7 A B Supplemental Figure C D