Lipid Absorption: Biochemical and Clinical Aspects

Transcription

1 Lipid Absorption: Biochemical and Clinical Aspects

2 Lipid Absorption: Biochemical and Clinical Aspects Proceedings of an International Conference held at Titisee, The Black Forest, Germany, May 1975 EDITED BY K. Rommel, University of Ulm and H. Goebell, University of Essen. Co-editor: R. Bohmer, University of Ulm MTP

3 Published by MTP Press Ltd St. Leonard's House Lancaster, England. Copyright 1976 MTP Press Ltd Softcover reprint of the hardcover 1st edition 1976 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior permission of the publishers. ISBN-13: e-isbn-13: DOl: /

4 Contents PART 1: PRINCIPLES OF LIPID ABSORPTION Fat digestion: the interaction of lipid digestion products with micellar bile acid solutions. A. F. Hofmann 3 2 The lipase-colipase system: P. Desnuelle 23 3 Properties of cell membranes and penetration by lipid molecules: D. Chapman 37 PART 2: NORMAL ASPECTS OF LIPID ABSORPTION 4 Uptake of fatty acid and monoglyceride: W. J. Simmonds 51 5 Phospholipid absorption: B. Borgstrom 65 6 Cholesterol absorption: N. Mcintyre 73 7 Triglyceride biosynthesis in the intestinal mucosa: J. M. Johnston 85 8 Chylomicron formation by the intestine: R. M. Glickman 99 9 Ultrastructural aspects of the intracellular assembly, transport and exocytosis of chylomicrons by rat intestinal absorptive cells: S. M. Sabesin 113 PART 3: ASPECTS OF LIPID ABSORPTION IN DISEASE STATES 10 Fat digestion and absorption in lipase and bile acid deficiency: J. R. Poley A-jJ-lipoproteinaemia: a model for studying the lipoprotein metabolism: G. M. Kostner 203 v

5 12 Fatty acid diarrhoea: relationships between diarrhoea and steatorrhoea: S. F. Phil/ips Aspects of lipid digestion and absorption in the ruminant: A. K. Lough Diagnosis of fat maldigestion and malabsorption: K. G. Wormsley 283 PART 4: SHORT COMMUNICATIONS 15 Effect of chemical modifications on the function of colipase: J. A. Barrowman, C. Erlanson and B. Borgstrom Are the lipases in human milk important for fat digestion in the newborn?: T. Olivecrona and O. Hernel/ Cholesterol absorption in patients with malabsorption: T. A. Miettinen Action of deoxycholate on the Semliki Forest virus membrane: A. Helenius and E. Fries Minimum protein requirements in patients with morbid obesity under the conditions of impaired lipid absorption due to jejunoileal bypass: J. Hasik, H. Hryniewiecki and T. Grala Some aspects of fatty acid absorption and metabolism by isolated epithelial cells from small intestine: G. Haag, H. Bierbach and A. W. Hol/dorf 335 General Discussion 343 vi

6 List of Participants BARROWMAN J. A. Memorial University of Newfoundland ST. JOHN'S Newfoundland AIC 5S7 BEISBARTH R. Firma Pfrimmer & Co 8520 ERLANGEN Postfach 91 BLOMSTRAND R. Karolinska Institutet. Department of Clinical Chemistry Huddinge University Hospital S HUDDINGE B6HMER R. Universitiit Ulm. Abteilung Klinische Chemie Neubau Ob. Eselsberg BORGSTR6M B. Inst. med. kemi 4 Department of Physiological Chemistry University of Lund Box 750 S LUND CASPARY W. Kliniken der Universitiit Gottingen Medizinische Klinik und Poliklinik 34 GOTTINGEN Humboldtallee 1 CHAPMAN D. Department of Chemistry The University of Sheffield SHEFFIELD S3 7HF England vii CLARK M. L. St. Bartholomew's Hospital LONDON EC1 England CLEMENT J. Dept. Clinical Physiology and Nutrition University of Dijon 2 Boulevard Gabriel 21 DIJON France DAWSON A. W. St. Bartholomew's Hospital LONDON EC1 England DESNUELLE P. Centre National de la Recherche Scientifique Centre de Biochemie et de Biologie Moleculaire 31. Chemin Joseph-Aiguier MARSEILLE Cedex 2 France DONNER J. University of Lund Department of Physiological Chemistry P.O. Box 750 S LUND 7 ECKNAUER R. Abteilung Klinische Chemie Universitiit Ulm, Ob. Eselsberg ERLANSON CH. Department of Physiological Chemistry University of Lund P.O. Box 750 S LUND 7

7 LIST OF PARTICIPANTS EWE K. Universitiitskliniken Mainz I. Med. Klinik und Poliklinik 65 MAINZ Langenbeckstr. 1 FORTH W. Ruhr-Universitiit Bochum Lehrstuhl fur Pharmakologie und Toxikologie 463 BOCHUM-QUERENBUR~ 1m Lottental GANGELT A. Aligemeines Krankenhaus der Stadt Wien Extraordinariat fur mediz. Computerwissenschaft und fur Gastroenterologie A 1097 WIEN Garnisongasse 13 Austria GLICKMAN R. M. Gastrointestinal Unit Massachusetts General Hospital BOSTON, Massachusetts GOEBELL H. Medizinische Universitiitsklinik Abt. fur Gastroenterologie 43 ESSEN Hufelandstr. 56 GRIMMEL K. Zentrum fur Innere Medizin der Universitiit Wurzburg 87 WURZBURG Josef-Schneider-Str. 2 HAAG G. Mediziniache Universitiitsklinik 78 FREIBURG Hugstetter StraBe 55 HAUTON J. Groupe de Recherches sur Ie transport des lipides 46 Chemin de la Gaye MARSEILLE France HELENIUS A. Europiiisches Laboratorium fur Molekularbiologie 69 HEIDELBERG Postfach HOFMANN A. F. Mayo Foundation, Gastroenterology Unit Saint Mary's Hospital ROCHESTER, Minnesota HOLLDORF A. W. Ruhr-Universitiit Bochum, Institut fur Physiologische Chemie Lehrstuhl II 463 BOCHUM-QUERRENBURG Universitiitsstr. 150, Gebiiude MA 4/141 HOLTERMOLLER K. H. Klinikum der Johann-Gutenberg U niversitiit I. Med. Klinik und Poliklinik 65 MAINZ Langenbeckstr. 1 HOTZ J. Zentrum fur Innere Medizin Sektion Gastroenterologie Universitiit Ulm Steinhovelstr. 9 JOHNSTON J. M. The University of Texas Health Science Center at Dallas Department of Biochemistry 5323 Harry Hines Blvd DALLAS, Texas HASIK J. Institute of Internal Medicine Medical Academy of Gastroenterology AI. Przybyszewskiego 49 POZNAN Poland viii KASPER H. Medizinische Universitiitsklinik Wurzburg Luitpolgkrankenhaus 87 WURZBURG Josef-Schneider-Str. 2

8 LIST OF PARTICIPANTS KOSTNER G. Institut fur Med. Biochemie der Universitat Graz A 8010 GRAZ U niversitatsplatz 2 Austria LOESCHKE K. II. Med. Klinik 8 MUNCHEN Ziemssenstr. 1 LOUGH A. K. Lipid Biochemistry Department The Rowett Research Institute Greenburg Road Bucksburn ABERDEEN AB2 9SB Scotland LUNDGREN O. Department of Physiology Goteborgs U.fliversitet S GOTEBORG 33 Medicinaregatan 11 MCINTYRE N. Medical Unit The Royal Free Hospital LONDON NW3 England MERKLE P. Abteilung fur Chirurgie Universitat Ulm Steinhovelstr. 9 MIETTINEN T. Second Department of Medicine University Central Hospital HELSINKI 29 Finland OLiVECRONA T. Div. Physiological Chemistry Universitet Dept. of Chemistry S UMEA RICKW. I. Med. Universitatsklinik 4 DUSSELDORF Moorenstr. 5 ix ROMMEL K. Abteilung fur Klinische Chemie Universitat Ulm Steinhovelstr. 9 RUMMEL W. Universitat des Saarlandes Institut fur Pharmakologie und Toxikologie 665 HOMBURG-SAAR PHILLIPS S. F. Mayo Foundation Gastroenterology Unit Saint Mary's Hospital ROCHESTER, Minnesota POLEY J. R. Department fur Kinderheilkunde des Zentrums fur Innere Medizin und Kinderheilkunde Universitat Ulm Prittwitzstr. 43 SABESIN S. M. Division of Gastroenterology Department of Medicine University of Tennessee MEMPHIS, TN SARDA L. Institut de Chimie Biologique Universite D'Aix-Marseilie I Place Victor-Hugo MARSEILLE Cedex 3 France SARLES H. Institut National de la Sante et de la Recherche Medicale 46 Chemin de la Gaye MARSEILLE France SCHEDL H. Department of Internal Medicine University Hospitals and Clinics IOWA CITY, Iowa SHMERLING H. Kinderspital Zurich Un iversitats-kinderklinik 8032 ZURICH Steinwiesstr. 75 Switzerland

9 LIST OF PARTICIPANTS SEUBERT W. Physiologisch-chemisches Institut der Georg~August-Universitat G6ttingen 34 GOTTINGEN Humboldtallee 7 SIMMONDS W. J. Department of Physiology The University of Western Australia NEDLANDS Western Australia SLEISENGER M. H. University of California 1120 HSW Department of Med. San Francisco Medical Center SAN FRANCISCO. Cal WEPLER R. Abteilung Klinische Chemie Universitat Ulm Steinh6velstr. 9 WINGATE D. L. The London Hospital Medical College Dept. of Physiology LONDON E1 England WORMS LEY K. G. Consultant Gastroenterologist Division of Medicine Ninewells Hospital DUNDEE DD2 1 US Scotland x

10 Foreword In May 1975 more than 50 scientists - research workers and clinicians - from 12 countries spent three days at Titisee in the Black Forest discussing problems relating to lipid absorption and ways of promoting closer liaison in the field. In this book all the papers presented at this symposium are published in full, together with the ensuing discussions. We should all like to thank Dr Falk for his generous sponsorship of this meeting which enabled us to work in such a charming atmosphere. We should also like to thank the authors and the other participants for the rapid preparation and correction of their manuscripts, and the publishers for their cooperation and assistance in preparing this book. We believe it represents a wide-ranging survey of existing knowledge of lipid absorption which will, we trust, provide a real stimulus for further research and new ideas in the study of this subject. K. Rommel H. Goebell R. Bohmer xi