4-YEAR DEGREE PROGRAMME I BIOCHEMISTRY YEAR I. A. Faculty & University Requirement. Compulsory Courses

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1 4-YEAR DEGREE PROGRAMME I BIOCHEMISTRY YEAR I A. Faculty & University Requirement Compulsory Courses Course Code Title Units FSC 101 Introductory Biology 3 FSC 102 Introductory Chemistry 3 FSC 103 Introductory Computer Science 3 FSC 104 Introductory Mathematics 3 FSC 105 Introductory Physics 3 GST 105 Use of English I 2 Total 17 B. Other Requirements for Biochemistry Majors Compulsory Course Course Code Title Units BCH 102/CHM 101 Introductory Chemistry II 4 BCH 103/CHM 102 Experimental Chemistry I 2 BCH 101/BTN 101 General Biology I 3 or BCH 106/ZLY 101 General Biology II 3 BCH 105/PHS 101 Introductory Physics II 2 or BCH 104/MAT 102 Calculus 2/3 GST 106 Use of English II 2 Total 13/14 C. Optional PHS 102 Introductory Physics III 3 PHS 103 Practical Physics 2 Course selection at this level 1. All students must register for all FSC courses in the first semester. 2. All Biochemistry majors must register for all compulsory courses for majors in the second semester in addition to any optional course to meet semester requirement.

2 YEAR II A. Compulsory for Biochemistry Majors BCH 201 Introductory Biochemistry (A) 2 BCH 205/CHM 203 Basic Organic Chemistry 4 BCH 206/CHM 204 Experimental Chemistry II (Physical & inorganic) 2 GAS 201 General African Studies 2 BCH 202 Introductory Biochemistry (B) 2 BCH 203/BIY 201 Cell Biology 3 BCH 204/CHM 202 Basic Physical Chemistry 4 BCH 207/CHM 205 Experimental Chemistry III (Organic) 2 GAS 202 General African Studies 2 Total 23 B. Optional CHM 201 Basic Inorganic Chemistry 4 PHS 202 Thermodynamics 2 ZLY 201 General Invertebrate Zoology 3 BIY 202/MIC 202 Introductory Development Biology 2 BIY 203 Introduction to the Biology of Micro organisms. 2 MAT 213 Pure Mathematics I 2 ZLY 202 Vertebrate Zoology 3 BIY 227 Plant Physiology 2 BIY 210 Biological Techniques 2 PHS 204 Modern Physics II 2 PHS 205 Oscillation & Waves 2 PHS 207 Optics 2 MIC 201 Introductory Microbiology 2 Courses can be selected from section B or other subject areas to meet the degree requirements. YEAR III A. Compulsory for Biochemistry Majors BCH 301 Chemistry of Macromolecules I (Protein & Nucleic acid). 2 BCH 302 Chemistry of macromolecules II 2 BCH 303 (Carbohydrates Lipid & Membrane) 2 BCH 307 Techniques in Biochemical Research 2 BCH 311/CHM 304 Organic reactions 3 BCH 312/CHM 306 Chemical Thermodynamics 2 BCH 313/CHM 314 Experimental Chemistry V 2 FSC 301 History & Philosophy of Science 2 Total 17

3 BCH 304 Intermediary Metabolism 3 BCH 305 Experimental Biochemistry II 2 BCH 306 Introductory Enzymology 2 BCH 309 Introductory Molecular Biology 2 FSC 302 Language (French/German) 2 Total 11 Elective: At least 4 units including MAT 317. MAT 317 Statistics for Biologists 3 BCH 308 Basic immunology 2 BCH 300 General Biochemistry (Non-Majors) 3 BCH 310 Biochemical Pharmacology 2 ZLY 307 Comparative Animal Physiology 4 BIY 302 Genetics 2 ZLY 310 Animal behaviour 2 OTHERS CHM 303 Methods of Chemical Analysis 2 CHM 305 Applied Spectroscopy 2 CHM 307 Chemical Kinetics 2 CHM 308 Electrochemistry 2 CHM 313 Expt. Chemistry IV (Physical Inorganic) 2 Courses can be selected from section B to meet the degree requirements. YEAR IV A. Compulsory for Biochemistry Majors BCH 401 Chemistry of Bio-polymer 3 BCH 402 Mechanism of Enzyme Action 3 BCH 403 Metabolism & Regulation 3 BCH 405 Experimental Biochemistry III 2 BCH 408 Seminars & Biochemical Literature 2 BCH 409 Bio membranes 2 BCH 404 Biochemical Genetics 2 BCH 420 Project 4 Total 19 B. Electives: At least 12 units selected from BCH 407 Nutritional Biochemistry 2 BCH 417 Biochemistry of Viruses 1

4 BCH 406 Biochemistry of hormones 2 BCH 410 Plant Biochemistry 2 BCH 411 Introduction to Biotechnology 2 BCH 412 Food Biochemistry 2 BCH 413 Advanced Immunology 2 BCH 414 Biochemistry of Parasites 2 BCH 415 Clinical Biochemistry 2 BCH 416 Biochemistry of selected organs & tissues 1 BCH 417 Biochemistry of viruses 1 Courses can be selected from section B or other subject areas to meet the degree requirement.

5 COURSE DESCRIPTIO 200 Level Courses BCH 201: Introduction Biochemistry I 2 Units Prerequisite: BCH 102, BTN 101 or ZLY 101. General considerations on the biological molecules of life (protein, carbohydrates, lipid, nucleic acid). General properties of enzymes as biological catalysts. Buffer and Buffer systems. Energy generation and storage in biological systems. (i) Harper, H.A. Review of Physiological Chemistry. (ii) Karlson P. Introduction to Modern Biochemistry. BCH 202: Introductory Biochemistry II 2 Units Prerequisite: BCH 201 Introduction of Metabolism and bioenergetics. Breakdown of biological molecules and their uses as fuels. Biological oxidation: Electron transport Chain, Phosphorylation, Physiological functions of hormones, Vitamins and cofactors, their importance in nutrition and metabolism. Unit and diversity in biochemistry. (i) Harper, H.A. Review of Physiological Chemistry. (ii) Karlson, P. Introduction to Modern Biochemistry (Academic Press). (iii) Conn & Stumpf. Outlines of Biochemistry. (Wiley & Sons). 300 Level Courses BCH 300: General Biochemistry ( on-majors) 3 Units General outlines of basic chemistry of biological Polymers, carbohydrates, proteins, lipids, nucleotides and polynucleotides. Elementary enzyme kinetics. Metabolism of lipids, amino acids and proteins. Metabolism of Carbohydrates, Glycolysis, TCA, etc Vitamins & cofactors. Special topics to include photosynthesis, hormones (plant & animal) detoxification mechanisms. (i) Conn & Stumpf. Outlines of Biochemistry. (ii) Sutie, J. W. Introduction to Biochemistry. (iii) Lehninger A. Principles of Biochemistry. BCH 301: Chemistry of Macromolecules 2 Units Prerequisite: BCH 201, BCH 202, CHM 203 General study of chemistry of proteins and nucleic acids. Functions of proteins, amino acid composition of proteins structure and properties. Classification of proteins based on solubility and structural organisation, determination of primary structure of proteins; N terminal and C terminal amino acids determination. Structure function-relationship of named proteins. Denaturation of proteins etc. Function of nucleic acids, evidence for

6 DNA as genetic material, primary structure of nucleic acids, structure of RNA & DNA. Properties of DNA in solution. Degradation of nucleic acids and sequencing of DNA. Texts: (i) Stryer, L. Biochemistry. (ii) Lehninger, A. Principles of Biochemistry. (iii) Wood, Wilson, Benbow & Wood. Biochemistry: A problem approach. (iv) McGilvery, R. W. Biochemical Concept. BCH: 302 Chemistry of Macromolecules II 2 Units Prerequisite: As for BCH 301 A general study of the Chemistry of Carbohydrates and lipids, including membrane lipids, including membrane lipids: classification and nomenclature of carbohydrates. Stereochemistry of carbohydrates, chemical reactions of carbohydrates, chemistry of polysaccharides. As for BCH 301 BCH 303: Experimental Biochemistry I 2 Units Prerequisite: As for BCH 302 and CHM 304 Buffer and buffer systems, ph indicators. Measuring techniques in Biochemistry cell fraction extraction, analysis and partial characterisation of macromolecules. Basic chromatographic techniques; approach for characterisation of macromolecules. BCH 304: Intermediary Metabolism 3 Units Prerequisite: BCH 302 Detailed metabolism of Carbohydrates and lipids, Gluconeogensis. Glyoxylate cycle. Hexosemono-phosphate shunt, fatty acid and triglyceride oxidation. Fatty acid and triglyceride synthesis, detailed treatment of the metabolism of amino acids degradation and biosynthesis; inborn errors of metabolism. Nucleoside, nucleotide and nucleic acid synthesis. One carbon metabolism, Transmethylation. Protoporphyrin Synthesis in animals and plants. Hormones and regulatory role in intermediary metabolism. As for BCH 301 BCH 305: Experimental Biochemistry II 2 Units Prerequisite: As for BCH 304 An introduction into methods of study of intermediary metabolism. Experimental approaches, synthesis of lipids, carbohydrate protein metabolism, lipid metabolism nucleic acid, metabolism, Electron transport and oxidative phosphorylation. Bruening, G. Biochemical Experiments. Criddel, R. BCH 306: Introduction to Enzymology 2 Units History of Enzymology, definition, enzyme as catalyst, general characteristics, nomenclature, nature of enzymes. Levels of structural organisation; active site, nature of

7 active site, enzyme kinetics, analytical enzymes - isolation, purification and characterisation of enzymes and measurement of enzyme activity - methods and principles. (i) Fersht. Enzyme structure & mechanism. (ii) Cornish-Bowden. Fundamentals of Enzyme Kinetics. (iii) Dawes, E.A. Quantitative Problem Biochem. BCH 307: Techniques in Biochemical Research 2 Units Prerequisite: CHM 205 Separation techniques in biochemistry. The Principles, procedures, and application of centrifugation, chromatographic separation and analytical techniques. The principles, instrumentation, and application of manometric, spectrofluorimetric spectrophotometric and radioisotope and analytical techniques. (i) Cooper, T.G. Tools of Biochemistry. John Wiley & Sons N.Y. (ii) Stock, R. & Rice, C.B.F. Chromatographic Methods. Chapman & Hall Ltd, London. BCH 308: Basic Immunology 2 Units Prerequisite: BCH 301, BCH 302 Basic immunobiology, Antigens, antibodies and their reactions. Classification and structural motifs of immunoglobulins. Antibody combining sites and structure function relationship complement system and fixation, organisation of immunoglobulin genes. Molecular basis of antibody diversity. Production, detection and uses of monoclonal antibodies. Concepts of clinical immunology; immune tolerance factors affecting immune response. Roitt: Essential Immunology (Blackwell Scientific publications). BCH 309: Introductory Molecular Biology 2 Units Prerequisite: BIY 201; BCH 301/BCH 302 A study of the structure and function of the genetic material in relation to genetic expression and control in prokaryotes and eukaryotes, including Cellular ultra structure: Chromosome structure and Gene expression: (i) Haggis, G.K. Introduction to Molecular Biology. (Longmans London). (ii) Hood, Wilson & Wood. Molecular Biology of Eukaryotic cells. Benjamin/Cummings Pub. Coy. BCH 310: Biochemical Pharmacology 2 Units Prerequisite: BCH 301, BCH 302 Introduction to pharmacological concepts. Emphasis on drug metabolism and drug interactions Ref: Mayer, Jawette & Goldher. Review of Medical Pharmacology.

8 BCH 401: Chemistry of Biological Polymers 2 Units Prerequisite: BCH 301, BCH 302 A comprehensive study of the structure and physico-chemical properties in solution of bio molecules. Amino acid structure and properties, primary structure of protein conformation and methods of analysis. Protein structure - function relationship. Review of Nucleic acid structure, nucleoproteins and nucleic acid isolation properties and conformation, polynucleotide sequencing, Polysaccharide and lipid structure, properties and analysis. Structural features of protoglycans, peptidoglycans, lipo-proteins and lipopolysaccharides. Membrane structure and integrity. (i) Lehninger, A. L. Principles of Biochemistry. (ii) Stryer, A.L. Biochemistry. (iii) Baker, R. Organic chemistry of biological compounds. BCH 402: Special Topics in Enzymology 2 Units Prerequisite: BCH 306 Enzyme structure and properties, structure of enzymes, enzyme assemblages, enzyme kinetics, principles of catalysis, multienzyme systems, allosterism, enzyme inhibition. Regulation of enzyme activity; chemical modification of enzyme activity, enzyme reaction mechanism. (i) Fersht. Enzyme Structure & Mechanism (ii) Cornish-Bowden. Fundamentals of Enzyme Kinetics. (iii) Dawes, E.A. Quantitative Problem in Biochemistry. BCH 403: Advanced Intermediary Metabolism and Regulation 3 Units Prerequisite: BCH 304 Advanced studies in degradation and synthesis of biological compounds. Carbohydrate degradation and synthesis and regulatory processes. Lipid, protein, purines and pyrimidine degradation and synthesis and regulatory process. Endocrine system and mechanism of hormone control of metabolism. (i) Larner, J. Intermediary metabolism. (ii) Stryer. Biochemistry. (iii) Lehninger, A.L. Principles of Biochemistry. BCH 404: Biochemical Genetics 2 Units Prerequisite: BCH 309 A more comprehensive study of the pathway of biological information transfer processes comprising detailed analysis of replication, transcription and translation. Mechanism of infection by virus. Recombinant - DNA and gene cloning, extra - chromosomal factors, plasmids, episomes. (i) Lehninger, A.L. Principles of Biochemistry. (ii) Stent & Cleender. Molecular Genetics. (iii) Woods, R.A. Biochemical Genetics. Outlines Studies in Biology.

9 (iv) Stryer, L. Biochemistry. (v) Watson, J.D., Hopkins, N.H., Roberts, J.H., Steitz, J.A. and Weiner, A.M. Molecular Biology of the gene. BCH 405: Experimental Biochemistry III 2 Units Prerequisite: Any two from BCH 303, BCH 305, BCH 307 Sets of experiments in Biochemistry to supplement BCH 401 BCH 402 & BCH 403. The course is designed to test student's ability in the use of biochemical literature and their ability to do simple experiments and interpret the resulting data (18 hours/week). BCH 406: Biochemistry of Hormones 2 Units Prerequisite: BCH 403 Evolution of hormone action, classes of hormones, intracellular mediators of hormone, hormone receptors, binding activity and response. Kinetics of binding, mode of action of hormones, camp as second messenger, role of calcium and other ions. Amino acid derived hormones, steroid hormones and polypeptide hormones. Hormone agonists, antagonists and partial agonists. (i) Lehninger, A.L. Principles of Biochemistry. (ii) Bhagavan, N.V. Biochemistry: A comprehensive Review. BCH 407: utritional Biochemistry 2 Units Prerequisites: BCH 301, 302 and BCH 304 Body composition, nutrients required by man and factors affecting the requirements. Foodstuff and their energy values and protein quality, food preservation and processing, food habits and intakes. Digestion, absorption, metabolism and function of carbohydrates, lipid, proteins and amino acids in man. Consequences of excess and inadequate calorie intake, protein-energy malnutrition. The vitamins, chemistry, metabolism and function, the minerals, metabolism and function. Animal and microbial nutrition. Feed formulation, food toxicants and detoxification mechanisms. (i) Stryer, L. Biochemistry. (ii) Pike, R.L. and Brown, M.L. utrition, "An integrated approach". (iii) Freedland and Briggs. A biochemical approach in utrition. BCH 408: Seminars & Biochemical Literature 2 Units Prerequisite: Any five from BCH 301 to BCH 309 Course to cover reading assignments to students which are expected to be completed during 3-6 hours library studies and later presented as short Seminars. One lecture weekly on growth and development of Biochemistry emphasizing major breakthrough in Biochemical research. BCH 409: Bio membranes 2 Units Prerequisite: BCH 302 Definition, list of functions and membrane types; membrane composition: - Lipid

10 structure, properties and formation of the bilayer; protein and carbohydrates. Membrane asymmetry and movements, diffusion, rotation and fluidity. Isolation and identification, electron microscopy and marker enzyme assays. Introduction to receptor function: - antigenicity of membrane components. Cell membranes and toxins, transport processes, mode of action of polymyxin and ionophores antibiotics. Introduction to neurotransmission. (i) Chairbone and Weissman. Biology of cell membrane. (ii) Fox, C.F. & Keith, A. Membrane molecular biology. BCH 410: Plant Biochemistry 2 Units Prerequisite: BCH 301, BCH 304 General description of sub cellular components of plant cell and methods of fractionation; Structure of primary cell wall. Complex carbohydrates of plants. Chemistry and metabolism. Autotrophy (Photosynthesis) Selected Topics in lipid metabolism. Aspects of nitrogen metabolism in plant. Mineral metabolism. Plant hormones. Bonner, J. & Verner, J.E. (Ed). Plant Biochemistry. BCH 411: Introduction to Biotechnology 2 Units Prerequisite: BCH 309 Introduction: Biochemistry of microbial growth, energetics and Kinetics of microbial growth. Down stream processing in Biotechnology and general instrumentation. Fermentation of Food related products. Microbial gums and other polymers. Enzyme production: immobilised enzymes and applications. Pollution control, genetic engineering; application in Biotechnology. Wisseman, A. (Ed). Principle of Biotechnology. BCH 412: Food Biochemistry 2 Units Prerequisites: BCH 301/302; BCH 304 Course covers biochemical changes in food - meat, fish, and fruits. Enzymatic browning and bio deterioration of food products - vegetable and oils, milk & milk products, cereal formulas and food additives. (i) Bell, Smith & Paterson. Textbook of Physiology and Biochemistry (9th edition - Livingstone). (ii) Smith, B. Textbook of Chemistry. (Avis Publication, 1975). BCH 413: Advanced Immunology 2 Units Prerequisite: BCH 302 BCH 304, BCH 308 This course covers the fundamentals of clinical immunology and mechanisms of immunopathology. Tissue reactions of injury, allergic disease and cancer immunology. Alexander, J.W. and Rover, A. Good. Fundamentals of Clinical Immunology. BCH 414: Biochemistry of Parasites 2 Units Prerequisite: BCH 301, BCH 302, BCH 304 Metabolism of bio molecules in parasites contrasted with that of the host, Host/parasite

11 interaction, and parasite nutrient needs. Biochemical mechanisms of drug action against parasitism. Defence mechanism in parasitism. Bio membranes of parasites. (i) W.E. Gutheridge, W.E. & Coombs, G.H.Q. Biochemistry of parasitic protozoa. (ii) Michael, F. Modern Genetic concepts and Techniques in the study of parasites. (iii) Hoelzi Wallach, D.F. The membrane pathobiology of Tropical diseases. BCH 415: Clinical Biochemistry 2 Units Prerequisite: BCH 303, BCH 305 Course covers the theoretical biochemical basis of laboratory tests of clinical significance in diagnosis and management of diseases. Curtius, B.S. & Roth Marc. Clinical Biochemistry & Principles methods, Vol. II. Walter De Gruyter, BCH 416: Biochemistry of Selected Organs and Tissues 1 Unit Prerequisite: BCH 301, BCH 302 Intracellular organisation, structure and functions of specialised tissues - liver, kidney muscles, adipose tissue, elastin, collagen, brain, Functional aspects of neural biochemistry membrane potential and ion transport. Neurotransmitters and biogenic amines in the brain. Constitution and function of blood, lymph and other fluids. Biochemistry of vision. BCH 417: Biochemistry of Viruses 1 Unit Prerequisite: BCH 301, BCH 309 Survey of structural properties of different classes of viruses. Viral multiplication mechanisms. Control of viral replication. Interferon. Styer. Biochemistry. BCH 420: Project 4 Units Prerequisites: Any two from BCH 303, BCH 305, BCH 307, Literature survey and planned laboratory work to be written up as a project under the supervision of a member of staff.