DO NOW: What is a catalyst?
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1 AGENDA ABSENT Block Nov 5 th /6 th -Week 13 TOPIC: Enzymes OBJ : 1-4 DO NOW: What is a catalyst? Science of Life EXT: Enzyme Lab DUE DATE: DW: Lab HANDOUTS to PICK-UP: -6.2 mini lab TURN IN to ABS box: SEMINAR 2: BEFORE/AFTER SCHOOL: DW-Review section of notes EXIT: Fill in your level of understanding AND list 3 things you learned from this lab!
2 BLOCK BLOCK: 11 / 5/6 OBJECTIVE #_1-4 TOPIC: Enzymes DO NOW Q: What is an catalyst? A: substance that lowers the amount of activation energy reaction can occur more easily Daily Work Grade- Tuesday Exit Activity DW GRADE: 2 1 0
3 Today s Objectives 1- --IDENTIFY / DIAGRAM parts of an atom 2- --DIFFERENTIATE & RECOGNIZE relationships of atoms, elements, molecules, compounds 3- --UTILIZE the periodic table to identify atomic structures of elements (CHOPNcharacteristics) 4- --COMPARE / CONTRAST covalent bonds and ionic bonds
4 IONS: atom that TRANSFERS electron lose an electron positive charge gain an electron negative charge CATION (+)Element gives up e- in the outer shell -atom becomes a positively charged ion ANION (-)Element gains the e- in their outer shell -atom becomes a negatively charge ion
5 REVIEW / PRACTICE p + n
6 REVIEW / PRACTICE
7 Lab Time! What is an Enzyme? -A biological molecule (proteins) that act as catalysts and help complex reactions occur everywhere in life What is a catalyst? -A substance that speeds up a reaction -helps reaction occur at a rate that is useful to cells
8 6.2 ENZYME LAB Does SALIVA have an affect on CARBOHYDRATES? -carbohydrates (aka starches)
9 FUNCTION of Carbohydrates provide your body with energy. FOUND: breads, pastas, candy, sodas, fruits. 6.2 ENZYME LAB energy component of diet: -biological compound -contains carbon, hydrogen, and oxygen [1:2:1] -important source of food and energy USE: -broken down and use as energy [glucose] -STORED in the liver and muscles. -glycogen. -when liver and muscles cells cannot store anymore glycogen -it is turned into fat. -body needs a quick boost of energy -converts glycogen into energy.
10 ENZYME found in saliva AMYLASE 6.2 ENZYME LAB -name describes its function -speeds up the breakdown of AMYLOSE (part in starch) -breaks down the carbohydrate before going into stomach
11 6.2 ENZYME LAB Question: Does SALIVA have an affect on CARBOHYDRATES? BACKGROUND: -6.2 notes (focus: activation energy / catalysts / enzymes) -INDICATORS -benedict's solution -test for the presence sugar. -heated: dark yellow color change indicates presence sugar (+ test). -iodine indicator -tests for the presence of starch. -purple-black color (+ test).
12 Demonstration Listen and take notes as I walk you through this lab!!
13 7 PRE-LAB IV-INDEPENDENT VARIABLE: DV-DEPENDENT VARIABLE: CONSTANTS: (be specific) EXPERIMENTAL GROUP: CONTROL GROUP: 6.2 ENZYME LAB
14 6.2 ENZYME LAB 10 PICTURE PROCEDURE *steps 4-8 -detail every step with IMAGE/PICTURE -ONLY KEY words used -review hot water bath -WEAR goggles
15 8 DATA TABLE -COLOR & +/- -detailed observation 6.2 ENZYME LAB TEST TUBE TT #1 cracker INITIAL observations TEST STARCH after INDICATOR color (+/-) after HEATING color (+/-) NA cracker TT #2 saliva TT #3 chewed cracker -mushy, cream colored,thick SUGAR -blue (-) NEG -blue (-)NEG SUGAR SUGAR
16 13 CONCLUSION 1- Does the cracker in test tube #1 contain starch? YES NO EXPLAIN- 6.2 ENZYME LAB 2- Does the saliva in test tube #2 contain sugar? YES NO EXPLAIN- 3-Does the chewed cracker in test tube #3 contain sugar? YES EXPLAIN- NO 4-INDICATE: Why did the cracker test positive for sugar AFTER it was chewed? 5-PROPOSE: What you think the addition of heat (water bath) did to the reaction occurring in test tube #3-6-HYPOTHESIZE: Do you think the reaction in test tube #3 would have occurred regardless of the addition of heat? 7- DEFINE/ IDENTIFY the following in the lab. CATALYST- EX BIOLOGICAL CATALYST- EX
17 6.2 CLEAN-UP ENZYME LAB 10% deduction 1-turn off hot plate [leave 250 ml beaker on HOT PLATE] 2-wash ALL test tubes with soap/scrubber 3-place CLEAN test tubes UPSIDE down in test tube rack to dry 4-set-up lab station for next group
![TIME Time FRAME Frame 20[m]](/docs-images/90/104492574/images/18-0.jpg "PRE-LAB / PICTURE PROCEDURE check")
![20[m] TEST / COLLECT DATA 5[m]](/docs-images/90/104492574/images/18-3.jpg "CLEAN-UP 10[m] CONCLUSION")
18 TIME Time FRAME Frame 20[m] PRE-LAB / PICTURE PROCEDURE check with Mrs. Cohan when COMPLETED GATHER GOGGLES/ START hot water bath 20[m] TEST / COLLECT DATA 5[m] CLEAN-UP 10[m] CONCLUSION [complete statements] WORK ON: 6.2 Vocab
19 EXIT FILL-IN: level of understanding of today s objective EXIT: List 3 things you learned from this lab today.
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