Fusarium oxysporum. Fusarium oxysporum 31. GORDON and MARTYN, 1997 F. oxysporum BOOTH, F. oxysporum. 100 formae specialis

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1 Fusarium oxysporum 31 Fusarium oxysporum Fusarium oxysporum GORDON and MARTYN, 1997 F. oxysporum BOOTH, 1971F. oxysporum 100 formae specialis BOOTH, 1971 F. oxysporum Di PIETRO et al., 2003 F. oxysporum n 1 mrna F. oxysporum Di PIETRO and RONCERO, 1996 ; 1998 ; ARIE et al., 1998 ; HUERTAS GONZÁLEZ et al ; RUIZ ROLDÁN et al ; GARCÍA The Seeking of Pathogenicity Related Genes in Fusarium oxysporum. By Masato KAWABE and Tsutomu ARIE MACEIRA et al., 2000 ; 2001 ; RONCERO et al., 2000 ; Di PIETRO et al., 2001 a ; 2001 b ; 2003 ; GÓMEZ GÓMEZ et al., 2001 ; 2002 ; NAMIKI et al., 2001 ; INOUE et al., 2002 ; JAIN et al., 2002 ; 2003 ; CARACUEL et al., 2003 ; MADRID et al., 2003 ; OSPINA GIRALDO et al., 2003 ; KAWABE et al., 2004 ; 2006 ; 2008 ; DUYVESTEIJN et al., 2005 ; REP et al., 2005 ; IMAZAKI et al., 2007 ; MARTÍN URDÍROZ et al., 2008 ; YOSHIDA et al., 2008 ; JONKERS et al F. oxysporum 1 F. oxysporum REP et al., 2004 ; HOUTERMAN et al., 2008 ; 2009 F. oxysporum F. oxysporum I KAWABE et al., 2004 ; YOSHIDA et al., 2008 Restriction Enzyme Mediated Integration REMI 31

2 Fusarium oxysporum ARG1 chsv chsvb FCD1 fga1 fgb1 fmk1 FoSNF1 FOW1 FOW2 FPD1 FRP1 PacC pg1 pg5 pgx4 pl1 Prt1 sap1 SIX1 SIX3 SIX4 xyl3 xyl3 xyl4 xyl5 argininosuccinate lyase class V chitin synthase class VII chitin synthase cellobiose : quinon oxydoreductase G protein alpha subunit G protein beta subunit MAP kinase protein kinase mitochondrial carrier protein transcription regulator transmembrane protein F box protein ph signaling transcription factor endopolygalacturonase endopolygalacturonase exopolygalacturonase pectate lyase subtilase aspartic proteinase cysteine rich protein unknown unknown NAMIKI et al., 2001 MADRID et al., 2003 ; MARTÍN URDÍROZ et al., 2008 MARTÍN URDÍROZ et al., 2008 KAWABE et al., 2006 JAIN et al., 2002 JAIN et al., 2003 Di PIETRO et al., 2001 a OSPINA GARALDO et al., 2003 INOUE et al., 2002 IMAZAKI et al., 2007 KAWABE et al., 2004 DUYVESTEIJN et al., 2005 ; JONKERS et al., 2009 CARACUEL et al., 2003 ARIE et al., 1998 ; Di PIETRO and RONCERO, 1996 ; 1998 GARCÍA MACEIRA et al., 2001 GARCÍA MACEIRA et al., 2000 HUERTAS GONZÁLEZ et al Di PIETRO et al., 2001 b YOSHIDA et al., 2008 REP et al., 2005 HOUTERMAN et al., 2009 HOUTERMAN et al., 2008 RUIZ ROLDÁN et al., 1999 GÓMEZ GÓMEZ et al., 2002 ; RUIZ ROLDÁN et al., 1999 GÓMEZ GÓMEZ et al., 2002 GÓMEZ GÓMEZ et al., 2001 RIGGLE and KUMAMOTO, 1998 F. oxysporum f. sp. lycopersicirace a 1 B pcsn43 REMI 1,500 DNA A A TAIL PCRLIU and WHITTIER, A A TAIL PCR REMI 1 r120 REMI REMI pcsn43 REMI r120 pcsn43 F. oxysporum TAIL PCR 32

3 Fusarium oxysporum AA 3 2 a a AA AA a 1 r120 d 9 d 17 d 25 e 19 e 21 p FPD a 1 r120 d 9 d 17d 25 FPD1 e19e21 ectopic Turkey p 0.05KAWABE et al AA TAIL PCR r120 REMI 2 1 FPD1 reverse trans criptase PCRRT PCR rapid amplification of cdna endsrace FPD1 GenBank acc. no. AB110097FPD1 47 bp 350 bp 613 bp 321 aa FPD1 chloride conductance regulatory proteinicln FPD1 F. graminearum Magnaporthe oryzaeneurospora crassa F. oxysporum FPD1 FPD1 2KAWABE et al., 2004 FPD1 FPD1 FPD1 33

4 II FPD1 HACKER and KAPER, 2000 ; BASSE et al., 2002 ; LIU et al., 2003FPD1 FPD1 KAWABE et al., 2006FPD1 FCD1 RT PCR RACE FCD1 acc. no. AB FCD1 46 bp 615 bp 1,017 bp 544 aa FCD1 CDH F. oxysporum FCD1 CDH CDH 3 c CDH CBQ CBQ c 3RAÍCES et al., 2002FCD1 CDH CBQ CBQ CDH FCD1 CBQ KAWABE et al., 2006 CDH Sclerotinia sclerotiorum BAMINGER et al., 2001 FCD1 F. oxysporum FCD1 MM CDH CBQ CDH FCD1 CDH CBQ FCD1 4FCD1 CBQ KAWABE et al., 2008 FCD1 CDH e e CBQ 3 CDHCBQ CDH CBQ KAWABE et al

5 Fusarium oxysporum 35 Units/100l CDHCBQ CDH A a a 1 d 10 p FCD a 1 d 10 FCD1 1M DCIP c 1 1U Turkey p 0.05KAWABE et al.2008 CDH CBQ FCD1 F. oxysporum Di PIETRO and RONCERO, 1996 ; 1998 ; ARIE et al., 1998 ; RUIZ ROLDÁN et al., 1999 ; GARCÍA MACEIRA et al., 2000 ; 2001 ; Di PIETRO et al., 2001 b ; 2003 ; GÓMEZ GÓMEZ et al., 2001 ; 2002 F. oxysporum CDH FCD1 F. oxysporum FPD1 FCD1 F. oxysporum B b C b 1ARIE, T. et al.1998: Ann. Phytopatholo. Soc. Jpn. 64 : BAMINGER, U. et al.2001: Appl. Environment. Microbiol. 67 : BASSE, C. W. et al.2002: Mol. Microbiol. 43 : BOOTH, C.1971: The genus Fusarium. Surrey : Commonwealth Mycological Institute. 5CARACUEL, Z. et al.2003: Mol. Microbiol. 48 : Di PIETRO, A. and M. I. G. RONCERO1996: Phytopathology 86 : : Mol. Plant Microb. Interact. 11 : et al.2001 a: Mol. Microbiol. 39 : et al.2001 b: Mol. Plant Microb. Interact. 14 : et al.2003: Mol. Plant Pahol. 4 : DUYVESTEIJN, R. G. E. et al.2005: Mol. Microbiol. 57 : GARCÍA MACEIRA, F. I. et al.2000: Mol. Plant Microbe Interact. 13 : et al.2001: Appl. Environ. Microbiol. 67 : GÓMEZ GÓMEZ, E. et al.2001: Curr. Genet. 40 : et al.2002: Fungal. Genet. Biol. 35 : GORDON, T. R. and R. D. MARTYN1997: Annu. Rev. Phytopathol. 35 : HACKER, J. and J. B. KAPER2000: Annu. Rev. Microbiol. 54 : HOUTERMAN, P. M. et al.2008: PLoS. Pathog. 4 : e et al.2009: Plant. J. 58 : HUERTAS GONZÁLEZ, M. D. et al.1999: Curr. Genet. 35 : IMAZAKI, I. et al.2007: Mol. Microbiol. 63 : INOUE, I. et al.2002: Plant Cell. 14 : JAIN, S. et al.2002: Curr. Genet. 41 : et al.2003: Curr. Genet. 43 : JONKERS, W. et al.2009: Mol. Plant Microbe Interact. 22 : KAWABE, M. et al.2004: J. Gen. Plant. Pathol. 70 : et al.2006: Gene 382 : et al.2008: Soil Microorganisms 62 : LIU, Y. G. and R. F. WHITTIER1995: Genomics 25 : LIU, X. et al.2003: Curr. Genet. 44 : MADRID, M. P. et al.2003: Mol. Microbiol. 47 : MARTÍN URDÍROZ, M. et al.2008: Eukaryot. Cell 7 : NAMIKI, F. et al.2001: Mol. Plant Microbe Interact. 14 : OSPINA GIRALDO, M. D. et al.2003: Curr. Genet. 44 : RAÍCES, M. et al.2002: Biochim. Biophys. Acta : REP, M. et al.2004: Mol. Microbiol. 53 : et al.2005: Mol. Plant microbe Interact. 18 : RIGGLE, P. J. and C. A. KUMAMOTO1998: Curr. Opin. Microbiol. 1 : RONCERO, M. I. G. et al.2000: Rev. Iberoam. Micol. 17 : S47 S53. 40RUIZ ROLDÁN, M. C. et al.1999: Mol. Gen. Genet. 261 : YOSHIDA, T. et al.2008: J. Pestic. Sci. 33 :