Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Blizard Institute Core Pathology Pathology and Pharmacy Building Second Floor 80 Newark Street London E1 2ES Contact: Pauline Levey Tel: +44 (0) Testing performed at the above address only Website: Fixed and fresh excisional and incisional biopsies and surgical resection specimens DETAIL OF ACCREDITATION Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of Specimen Dissection SOPS [195 - Cut up [194 - Decalcification of Specimens [193 - Specimen Dissection] [322 Products of Conception [335 Dissection of Heart Ventricular Slice] Tissue processing and embedding using Thermo Shandon Excelsior Tissue Processors and Thermo Shandon embedding centre [83 - Processing of Specimens [213-Embedding of Specimens Assessment Manager: AB2 Page 1 of 10

2 Formalin Fixed Paraffin wax blocks of processed tissue. Fresh/frozen tissue and frozen sections Intraoperative examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of Routine morphological staining for the detection of basophilic and eosinophilic structures Microtomy using Thermo Shandon finesse rotary microtomes and RA Lamb sledge microtome [209-Cutting Paraffin Sections Using Thermo Shandon Cryostat FE Automated methods using Thermo Shandon Gemini Manual staining method Thermo Shandon Coverslipper [215-Frozen Section [30 Haematoxylin and Eosin for Frozen Sections] 32 - Haematoxylin and Eosin [629 Core Pathology Staining Manual] Assessment Manager: AB2 Page 2 of 10

3 Special stains for the detection of: Documented in-house procedures for manual and automated special stains [629 Core Pathology Staining Manual] Acid and neutral mucins Polysaccharides and fungi Polysaccharides including glycogen and fungi Acid Mucins Amyloid Nissl components and Helicobacter Melanin, argentaffin, chromaffin and some lipofuschin Haematopoietic cells Reticulin fibres Automated method using Thermo Shandon Gemini 582 ABPAS Automated method using Thermo Shandon Gemini 75 DPAS Automated method using Thermo Shandon Gemini 75 PAS 2 - Alcian Blue 12 - Congo Red 13 - Cresyl Fast Violet 18 Fontana 21 Giemsa 25 - Gordon and Sweet Assessment Manager: AB2 Page 3 of 10

4 Special stains for the detection of: Gram positive and negative bacteria Fungi, pneumocystis Basement membranes Myelin, lipofuscin and nissl granules Connective tissue Elastic fires and connective tissue Fibrin connective tissue Unsaturated hydrophobic lipids and phospholipids Haemosiderin Connective tissue Nissl granules and mast cells Documented in-house procedures for manual and automated special stains 26 Gram 28 Grocott 35 - Hexamine Silver 37 - Luxol Fast Blue Cresyl Fast Violet 39 - Masson Trichrome 43 - Millers Elastic 44 MSB 48 - Oil Red O 55 - Perls Prussian Blue 57 - Picro Sirius 66 - Toluidine Blue Assessment Manager: AB2 Page 4 of 10

5 Special stains for the detection of: Nuclei and connective tissue HBsAg, elastic and copper associated protein Calcium Mycobactrium leprae Mycobacterium spp including tuberculosis Immunohistochemistry to detect the following: Epithelial cells T-cells, B-cells in the mantle zone Follicular centre B-cells Epithelial cells Documented in-house procedures for manual and automated special stains 67 - Van Gieson 69 - Victoria Blue 70 - Von Kossa 71 - Wade Fite 74 - Ziehl Neelson Roche Ventana Benchmark Using the following antibodies [629 Core Pathology Staining Manual] AE1/3 Bcl-2 Bcl-6 Ber-EP4 Assessment Manager: AB2 Page 5 of 10

6 Immunohistochemistry to detect the following: Endothelium and basement membrane showing remnant of C4d complement. Mesothelial cell lining Epithelial cells of the colon T-cell marker T-Helper cells T-cells, B-cells in the mantle zone Cytotoxic T-cells Immature B lymphocytes B-cell marker Mature B-cells and FDC Bcells RS cells Endothelial cells Endothelial marker Leukocyte common antigen N killer (NK) cells, NK like T-cells, neural cell adhesion molecule Macrophages Plasma cells Roche Ventana Benchmark Using the following antibodies C4d Calretinin CAM5.2 CD3 CD4 CD5 CD8 CD15 CD20CY CD21 CD23 CD30 CD31 CD34 Class II CD45 CD56 CD68 CD138 Assessment Manager: AB2 Page 6 of 10

7 Immunohistochemistry to detect the following: Intestinal epithelial Carcinoembryonic antigen Neuroendocrine Cytomegalovirus Mantle cell lymphoma Roche Ventana Benchmark Using the following antibodies CDX-2 CEA(P) Chromogranin A Cytomeglavirus (CMV) Cyclin D1 Squamous and myoepithelial Cytokeratin 5/6 Glandular, transitional epithelial Cytokeratin 7 Gastrointestinal epithelia Cytokeratin 20 Lymphatic endothelium. Intermediate filament GIST Epithelial membrane antigen Oestrogen receptor Helicobacter Human herpesvirus type 8 Melanosomes Herpes simplex virus Proliferation marker Melanocytes D2-40 (Podoplanin) Desmin DOG-1 EMA (Epithelial Membrane Antigen) ER Helicobacter HHV8 HMB45 (Melanosome) Herpes Simplex Virus Type I Ki67 Melan A Assessment Manager: AB2 Page 7 of 10

8 Immunohistochemistry to detect the following: 40kD epithelial cell adhesion molecule B-cells Neuroendocrine marker Cervical Ca Squamous and basal cell carcinoma s Tumour suppressor gene Basal cell marker Progesterone receptor Melanoma, neuroendocrine Mesenchymal marker, smooth muscle cells Simian virus 40 Neuroendocrine marker Lumen of thyroid follicles Thyroid transcription factor-1 Mesenchymal marker Wilms tumour gene product Roche Ventana Benchmark Using the following antibodies MOC 31 MUM-1 NSE P16 P40 P53 P63 PR S100 SMA SV40 Synaptophysin Thyroglobulin TTF-1 Vimentin WT-1 Assessment Manager: AB2 Page 8 of 10

9 Skin biopsies Immunohistochemistry to detect the following: Mast cells and GIST Mitochondria Broad range cytokeratin Diagnosis of bullous skin disorders, connective tissue disease (SLE, DM), or vasculitis by detection of deposition of immunoglobulins, complement proteins and fibrinogen: DAKO Autostainer Using the following antibodies [356-DAKO Autostainer Universal Staining System User Manual] [629 Core Pathology Staining Manual] CD117 Mitoc MNF116 Immunofluorescence using Ventana Benchmark system Thermo Shandon Cryostat FE Hamamatsu Slide Scanner [577-Slide scanning manual] [629 Core Pathology Staining Manual] Gamma chains Alpha chains Mu chains C3c fragment of complement Fibrinogen IgG fluorescein isothiocyanate IgA fluorescein isothiocyanate IgM fluorescein isothiocyanate C3c fluorescein isothiocyanate Fibrinogen fluorescein isothiocyanate Assessment Manager: AB2 Page 9 of 10

10 Slides prepared as above HUMAN BODY FLUIDS Stained Non-Gynae Cytology slides Morphological assessment and interpretation/ Cytological examination activities for the purposes of clinical Morphological assessment and interpretation/ Interpretive Microscopy using an assorted range of diagnostic brightfield microscopes [628 General Light Microscope Operation] [405- Reporting Protocol for Consultant Pathologists] [191 Reception of Specimens [390 Tissue Pathways for Exfoliative Cytology and Fine Needle Aspiration Cytology] END Assessment Manager: AB2 Page 10 of 10