Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Paediatric Histopathology Western Bank Sheffield S10 2TH United Kingdom Contact: Tel: +44 (0) Fax: +44 (0) Website: Additional site activity performed away from the laboratory Testing performed at the above address only Location details Activity On-site Mortuary and Body Store Storage of bodies Retrieval of tissue via post mortem Record keeping (body identification and audit trail) Assessment Manager: RBB Page 1 of 14

2 DETAIL OF ACCREDITATION HUMAN BODY TISSUE resection specimens diagnosis purpose of diagnosis Specimen Dissection SOP General specimen reception procedures. SOP Photography QPulse Asset module - HIS023 - HIS161 SOP Surgical samples - cut up SOP Handling fresh samples SOP Tumours & Lymph nodes (lymphomas) SOP Macro micro form SOP Gastrointestinal biopsy macro sheet SOP Surgical samples - cut up Processing Thermo Shandon Excelsior Tissue Processor & Leica ASP300S SOP Tissue Processing schedules SOP Tissue processor - General use Microtomy Leica Microtomes for sectioning blocks to produce 4um thick ribbons Assessment Manager: RBB Page 2 of 14

3 Unfixed Body fluids Unfixed Cell washings Unfixed Fine Needle Aspirates Cell slide preparations Cell block preparations purpose of Special stains to identify the following: Acid Mucins Mucins Amyloid Mast cells and white cells (granulocytes), of myeloid lineage Microtomy RA Lamb and Thermo Shandon Water baths set at 45⁰C for floating sections SOP Microtomy Non Gynae Cytology SOP Types of cytology specimens SOP Receipt of cytology and Surgical specimens SOP Cytospin preparation Haematoxylin and Eosin stain Automated and manual Leica AutoStainer XL automated H&E stainer Leica CV 5030 Coverslipper automated coverslipper SOP Haematoxylin & Eosin SOP Leica Autostainer XL & Coverslipper CV5030 Special staining Manual SOP Alcian Blue/PAS SOP Alcian Blue SOP A Alcian blue ph 1.0 SOP Congo Red SOP Chloroacetate Esterase - Leder's Assessment Manager: RBB Page 3 of 14

4 purpose of Special stains to identify the following: (cont d) Remove deposits of mineral Micro-organisms and Protozoa Elastic fibres Reticulin fibres Special staining Manual SOP EDTA SOP Giemsa SOP Elastic Stain (Modified Miller's) SOP Gordon and Sweet's Reticulin Gram +ve micro-organisms Gram +ve and -ve micro-organisms Argyrophil cells Fungi and Pneumocystis carinii Myelin Fibrin SOP Gram +ve SOP Gram Twort Method SOP Grimelius Silver Method SOP Grocott's Silver Technique SOP Grocott Methanamine Silver rapid Method SOP Luxol Fast Blue standard method SOP Luxol Fast Blue/Cresyl Fast Violet SOP MSB (Martius Yellow, Crystal Scarlet, Soluble Blue) Assessment Manager: RBB Page 4 of 14

5 purpose of Special stains to identify the following: (cont d) Melanin Connective tissues Hematopoietic cells Basement membranes Helicobacter Copper associated protein, elastic and Hepatitis B surface antigen (HBSAg) Glycogen, Basement membranes Fungi Glycogen Viral inclusions and keratin Astrocytes, Neuroglial cells and Fibres Removal of pigments Lipofucsin Special staining Manual SOP Masson's Fontana SOP b Masson's Trichrome Blue method SOP May Grunwald Giemsa SOP MST (Methanamine Silver Technique) Jones SOP Giemsa (Modified) Helicobacter SOP Shikata's (Modified Orcein) SOP PAS (Periodic Acid Schiff) SOP PAS - Diastase SOP Phloxine Tartrazine SOP PTAH for Glia SOP Pigments (Removal of) SOP Sudan Black Assessment Manager: RBB Page 5 of 14

6 purpose of Special stains to identify the following: (cont d) Special staining Manual Urine Mast cells SOP Toluidine Blue Metachromatic granules Calcium Mycobacterium leprae Spirochaetes and other non filamentous bacteria Acid and alcohol fast bacilli Nuclear stain Iron Melanin Connective tissues SOP Toluidine Blue for Metachromatic Dystrophy SOP Von Kossa SOP Wade Fite SOP Warthin Starry SOP Ziehl-Neelsen (ZN) SOP Weigert's Iron Haematoxylin SOP Perl's Prussian Blue SOP Masson Hamperl (Singh's Modification) SOP Picro Sirius Red Special staining (Non-Gynae Cytology) Manual Metachromatic leuko-dystrophy Glycogen SOP Toluidine blue for metachromatic leuko-dystrophy SOP PAS (Periodic Acid Schiff) Assessment Manager: RBB Page 6 of 14

7 purpose of Special stains to identify the following: (cont d) Routine staining for identification of basophilic and eosinophilic structures Fungi and Pneumocystis carinii Special staining (Non-Gynae Cytology) Manual SOP Haematoxylin & Eosin SOP Grocott's Silver Technique Immunohistochemistry Automated Dako Autostainer Link 48 automated stainer for use with a wide range of antibodies SOP Dako Autostainer Link 48 SOPs that cover Immunocytochemistry are to The main ones are: SOP Immunocytochemistry general procedures SOP Immunocytochemsitry reagents SOP Control material for Immunocytochemistry SOP UKNEQAS ICC SOP Criteria for the rejection of immunocytochemistry Assessment Manager: RBB Page 7 of 14

8 purpose of Immunocytochemistry for the identification of: Smooth Muscle/ myofibroblast Adenovirus Alpha1 antitrypsin genetic diseases/ various tumours Cytogenetic marker Some lymphomas Heptocellular carcinoma/ germ cell tumours Diagnostic utility lymphomas/ bone marrow biopsies Axonal damage Various carcinomas Smooth muscle Mesothelial marker, some tumours and Hirschsprungs Early hypoxic cellular damage Diagnostic utility lymphomas/ bone marrow biopsies Immunohistochemistry Automated And antibodies as appropriate:- Actin (Smooth muscle) Adenovirus Alpha1 Anti trypsin AE1&3 (Cytokeratin) ALK1 Protein Alpha Feto Protein BCL-2 BCL-6 Beta-APP Beta Catenin Caldesmon Calponin Calretinin C9 CD1a (010) Assessment Manager: RBB Page 8 of 14

9 purpose of Immunocytochemistry for the identification of: (cont d) Diagnostic utility lymphomas/ bone marrow biopsies (cont d) Immunohistochemistry Automated And antibodies as appropriate:- CD3 CD4 CD7 CD8 CD10 (Calla) CD15 CD20 (L26) CD21 (1F8) CD23 CD30 (Kil) Ber H2 CD44 CD45 (LCA) CD45RO (UCLH1) CD56 (NCAM) CD61 CD68 (PGM1) CD79a Assessment Manager: RBB Page 9 of 14

10 purpose of Immunocytochemistry for the identification of: (cont d) Diagnostic utility lymphomas/ bone marrow biopsies (cont d) Endothelial marker Stromal cells GIST, Haematopoietic progentitor, Mast cells Immunohistochemistry Automated And antibodies as appropriate:- CD99 CD31 CD34 (QB-End10) CD117 (C-KIT) Bone Biopsies/ Erythroid cells CD235a (Glycophorin A) Intestinal metaplasia/ Cancer colorectal origin Neuroendocrine differentiation tumours/ medullary carcinoma thyroid Identification primary site carcinomas Cytomegalovirus CDX2 Chromogranin A CK7 CMV Lymphatic endothelium D2-40 Smooth muscle marker Muscle biopsy Desmin D33 DYS1 DYS2 DYS3 Assessment Manager: RBB Page 10 of 14

11 purpose of Immunocytochemistry for the identification of: (cont d) Most normal and neoplastic epithelium/ some lymphomas Endothelial markers Dermal dendrocytes (skin/ soft tissue markers) Astrocytes, schwann cells and pheripheral nerve tumours Infantile haemangioma Trophoblast/ germ cell tumours Be cells, B lineage ALL, most nonhodgkins lymphomas Immunohistochemistry Automated And antibodies as appropriate:- EMA Factor VIII Factor XIIIa GFAP GLUT-1 HCG HLA-DR H. Pylori H. Pylori Herpes Epitheliod sarcoma, atypical teratois/ rhabdoid tumour Tumour cell proliferation Mast cells Muscle biopsies Neutrophils, Granulocytic sarcoma HSV INI-1 Ki67 Mast Cell Tryptase (MCT) Merosin (M-Chain) Myeloperoxidase Assessment Manager: RBB Page 11 of 14

12 purpose of Immunocytochemistry for the identification of: (cont d) Differentation hepatocellular carcinoma from cholangiocarcinoma Skeletal muscle differentiation Muscle biopsy Skeletal muscle differentiation Be cell lineage/ Lymphoma Neuroblastoma/ Small round cell tumour Neurofilament protein Neurosecretery cells Parvovirus Immunohistochemistry Automated And antibodies as appropriate:- MOC 31 Myo-D Myosin Slow Myogenin MUM-1 NB84 NFP NSE Parvovirus Neuroendocrine differentiation PGP 9.5 Trophoblastic tumours Muscle biopsy PLAP Sarcoglycan Alpha Sarcoglycan Beta Sarcoglycan Gamma Spectrin Assessment Manager: RBB Page 12 of 14

13 Fresh Human Tissue purpose of Immunocytochemistry for the identification of: (cont d) Muscle biopsy (cont d) Differential skin, soft tissue & neural lesions Neuroendocrine marker Differential leukaemia/ lymphomas Differential for a variety of benign lesions and tumours Wilm's tumour Examination of fresh frozen tissue to rapidly identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Routine staining for identification of basophilic and eosinophilic structures Connective tissue Glycogen, Basement membranes Fungi Immunohistochemistry Automated And antibodies as appropriate:- Myosin Slow Myosin Fast S100 Synaptophysin TdT Vimentin Wilm's Tumour (WT1) Leica CM1900 & Leica CM1850 used for sectioning frozen fresh tissue SOP Haematoxylin & Eosin SOP Van Gieson Frozen Section SOP Cell PAS Frozen Section SOP Cryotomy Assessment Manager: RBB Page 13 of 14

14 purpose of diagnosis Interpretive Microscopy Nikon Microscope Olympus Microscope SOP UKNEQAS Immunocytochemistry SOP UKNEQAS Cellular Pathology - General Pathology Module. SOP a - UKNEQAS Cellular pathology - Muscle Histochemistry. SOP Internal assessment of quality of Histological Preparations. SOP Writing Surgical reports. SOP Revised Reports. SOP Reporting frozen sections. SOP a - Muscular biopsy report. SOP Oncology reporting. SOP Neonatal/ Child post mortem report. SOP Coroners post mortem report. SOP Fetal/ Perinatal post mortem report. SOP Macroscopic description of singleton placenta. SOP Macroscopic description of placenta - twin SOP a - Placental histology comments END Assessment Manager: RBB Page 14 of 14